{"title":"离体肌原纤维的体积变化","authors":"R.J. Baskin","doi":"10.1016/0926-6577(64)90095-6","DOIUrl":null,"url":null,"abstract":"<div><p>Volume changes in glycerol-extracted myofibrils were studied. The effect of pH, ion content and ATP concentration on the change in volume was determined. A volume change greater than that attributed to ATP dephosphorylation was observed. The magnitude of the volume change of the entire system was 10<sup>−3</sup> cm<sup>3</sup> whereas that due to dephosphorylation was 10<sup>−6</sup> cm<sup>3</sup>. The ratio of ATPase activity in a volume decrease to that in a volume increase was found to be approx. 10:1. The total amount of volume decrease measured approx. 0.028 cm<sup>3</sup>/g of muscle with a fresh myo fibril preparation. The volume increase which was observed upon the addition of large amounts of ATP amounted to approx. 0.036 cm<sup>3</sup>/g of muscle. The relationship between the volume changes and molecular reorganization of the myofibril proteins was discussed. It was concluded that either a large configurational change occurs in the myofibril proteins during an ATP-induced contraction or relaxation, or that the volume changes are related to the dissociation and association of actin and myosin filaments</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":"88 3","pages":"Pages 517-527"},"PeriodicalIF":0.0000,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90095-6","citationCount":"5","resultStr":"{\"title\":\"Volume changes in isolated myofibrils\",\"authors\":\"R.J. Baskin\",\"doi\":\"10.1016/0926-6577(64)90095-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Volume changes in glycerol-extracted myofibrils were studied. The effect of pH, ion content and ATP concentration on the change in volume was determined. A volume change greater than that attributed to ATP dephosphorylation was observed. The magnitude of the volume change of the entire system was 10<sup>−3</sup> cm<sup>3</sup> whereas that due to dephosphorylation was 10<sup>−6</sup> cm<sup>3</sup>. The ratio of ATPase activity in a volume decrease to that in a volume increase was found to be approx. 10:1. The total amount of volume decrease measured approx. 0.028 cm<sup>3</sup>/g of muscle with a fresh myo fibril preparation. The volume increase which was observed upon the addition of large amounts of ATP amounted to approx. 0.036 cm<sup>3</sup>/g of muscle. The relationship between the volume changes and molecular reorganization of the myofibril proteins was discussed. It was concluded that either a large configurational change occurs in the myofibril proteins during an ATP-induced contraction or relaxation, or that the volume changes are related to the dissociation and association of actin and myosin filaments</p></div>\",\"PeriodicalId\":100169,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects\",\"volume\":\"88 3\",\"pages\":\"Pages 517-527\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1964-11-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6577(64)90095-6\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926657764900956\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926657764900956","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Volume changes in glycerol-extracted myofibrils were studied. The effect of pH, ion content and ATP concentration on the change in volume was determined. A volume change greater than that attributed to ATP dephosphorylation was observed. The magnitude of the volume change of the entire system was 10−3 cm3 whereas that due to dephosphorylation was 10−6 cm3. The ratio of ATPase activity in a volume decrease to that in a volume increase was found to be approx. 10:1. The total amount of volume decrease measured approx. 0.028 cm3/g of muscle with a fresh myo fibril preparation. The volume increase which was observed upon the addition of large amounts of ATP amounted to approx. 0.036 cm3/g of muscle. The relationship between the volume changes and molecular reorganization of the myofibril proteins was discussed. It was concluded that either a large configurational change occurs in the myofibril proteins during an ATP-induced contraction or relaxation, or that the volume changes are related to the dissociation and association of actin and myosin filaments
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