埃利希腹水癌细胞表面及细胞质膜的研究

Donald F. Hoelzl Wallach, Donna Ullrey
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引用次数: 35

摘要

从埃利希腹水癌微粒体分离的膜片段含有ATP磷酸水解,在Na+存在的情况下,它受到K+的强烈刺激。酶系统对Na+和Mg2+有绝对的需求,但一些一价阳离子可以模拟K+。Ca2+、ADP和瓦巴因均有抑制作用。这种酶被认为与质膜碎片有关。提出了酶活性与反应物浓度之间的定量关系,并讨论了全细胞内离子转运与碱阳离子敏感的ATP磷酸水解酶之间的可能关系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Studies on the surface and cytoplasmic membranes of Ehrlich ascites carcinoma cells

A membrane fraction isolated from Ehrlich ascites carcinoma microsomes contians an ATP phosphohydrolas which is strongly stimulated by K+ in the presence of Na+. The enzyme system has an absolute requirement for Na+ and Mg2+, but a number of monovalent cations can simulate K+. Ca2+, ADP and ouabain are inhibitory. The enzyme is believed to be associated with plasma membrane fragments.

The quantitative relationships between enzyme activity and reactant concentration is presented and the probable relationship between ion transport in the whole cell and the alkali-cation-sensitive ATP phosphohydrolase is discussed.

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