Potassium transport in Neurospora

Carolyn W. Slayman , E.L. Tatum
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引用次数: 135

Abstract

  • 1.

    1. Wild-type Neurospora crassa, growing logarithmically in minimal medium, contains 180 ± 16 mM intracellular K+ and 14 ± 2 mM Na+. It requires K+ but not Na+ for growth. Over a wide range of extracellular K+ concentrations (0.3 to 100 mM), the cells maintain a constant, high level of intracellular K+ (180 mM) and rapid growth occurs. Below 0.3 mM, the internal K+ concentration falls and growth is inhibited.

  • 2.

    2. When cells are harvested from logarithmic growth and resuspended in distilled water or in 1 mM sodium azide, they show no significant loss of internal K+ for at least 1 h. Exposure to the polyene antibiotic nystatin (30 μg/ml) or the decapeptide antibiotic tyrocidine (20 μg/ml) causes most of the internal K+ to be lost within 2 min. Both antibiotics are thought to attack the plasma membrane of sensitive organisms.

  • 3.

    3. Neurospora exchanges its intracellular K+ quite rapidly for extracellular K+ or Rb+, but only slowly for Na+.

  • 4.

    4. These experiments were undertaken to provide a backgroun for microelectrode studies with Neurospora and for the isolation of a K+-transport mutant.

钾在神经孢子虫中的转运
1.1. 野生型粗神经孢子菌在最小培养基中呈对数生长,细胞内K+含量为180±16 mM, Na+含量为14±2 mM。它需要K+而不需要Na+来生长。在细胞外K+浓度的大范围内(0.3至100 mM),细胞保持恒定的高水平细胞内K+ (180 mM),并发生快速生长。在0.3 mM以下,内部K+浓度下降,生长受到抑制。当细胞从对数生长中收获并在蒸汽水或1mm叠氮化钠中重悬时,它们在至少1小时内没有显示出内部K+的显著损失。暴露于多烯抗生素制霉菌素(30 μg/ml)或十肽抗生素酪胺(20 μg/ml)会导致大部分内部K+在2分钟内损失。这两种抗生素被认为会攻击敏感生物体的质膜。神经孢子菌胞内K+与胞外K+或Rb+的交换速度较快,而Na+的交换速度较慢。这些实验是为神经孢子菌的微电极研究和K+转运突变体的分离提供了背景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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