Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis最新文献_第5页

Effect of diquat (1,1′-ethylene-2,2′-dpyridylium dibromide) on the formation and photoreactions of chromatophores from Rhodospirillum rubrum diquat(1,1′-乙烯-2,2′-二溴二吡啶)对红红螺旋藻色素团形成及光反应的影响

Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-10-10 DOI: 10.1016/0926-6585(66)90058-6

Tsuneo Kaneshiro , Gunter Zweig

{"title":"Effect of diquat (1,1′-ethylene-2,2′-dpyridylium dibromide) on the formation and photoreactions of chromatophores from Rhodospirillum rubrum","authors":"Tsuneo Kaneshiro , Gunter Zweig","doi":"10.1016/0926-6585(66)90058-6","DOIUrl":"https://doi.org/10.1016/0926-6585(66)90058-6","url":null,"abstract":"<div>Analysis of chromatophore suspensions fractionated from Rhodospirillum rubrum indicated that the diphenylamine did not interfere with the cellular synthesis of bacteriochloropyll (absorption at 775 mμ in acetone-methanol). Diquat, on the other hand, interfered with the synthesis of both bacteriochlorophyll within chromatophore suspensions and the detectable Rhodospirillum heme protein of the supernatant (absorption at 435 mμ). While photoreduction of O2 was dependent on substrates such as malate or succinate, photophosphorylation by chromatophore suspensions was mostly independent of these substrates under aerobic conditions. Under anaerobic conditions, ascorbate plus 2,6-dichlorophenolindophenol caused an “ovver-reduced” environment in which photophosphorylation was inhibited but was restored by diquat. The photophosphorylation altered by reduced 2,6-dichlorophenolindophenol and phenazine methosulfate seemed to be related to the presence of atmospheric O2; the stimulation by phenazine methosulfate was dependent on both siccinate and O2;</div>","PeriodicalId":100158,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","volume":"126 2","pages":"Pages 225-233"},"PeriodicalIF":0.0,"publicationDate":"1966-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6585(66)90058-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91674954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Ultracentrifugal behavior of transferrin in the presence of some anions 转铁蛋白在阴离子存在下的超离心行为

Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-10-10 DOI: 10.1016/0926-6585(66)90065-3

Anatoly Bezkorovainy

引用次数: 3

On the occurrence of a contractile protein in spinach chloroplasts 一种收缩蛋白在菠菜叶绿体中的发生

Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-10-10 DOI: 10.1016/0926-6585(66)90077-X

Joyce A. Young, Lester Packer

引用次数: 5

Temperature and photosynthesis 1. Some effects of temperature on carbon dioxide fixation by isolated chloroplasts 温度与光合作用温度对离体叶绿体固定二氧化碳的影响

Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-10-10 DOI: 10.1016/0926-6585(66)90056-2

C.W. Baldry, C. Bucke, D.A. Walker

{"title":"Temperature and photosynthesis 1. Some effects of temperature on carbon dioxide fixation by isolated chloroplasts","authors":"C.W. Baldry, C. Bucke, D.A. Walker","doi":"10.1016/0926-6585(66)90056-2","DOIUrl":"https://doi.org/10.1016/0926-6585(66)90056-2","url":null,"abstract":"<div><ul><li>1.1. Isolated pea chloroplasts were used to determine effects of temperature on photosynthetic CO2 fixation in saturating light and high CO2.</li><li>2.2. Increases in temperature between 5° and 30° increased the maximum rate and shortened an initial induction period. The maximum rate was not an exponential function of temperature.</li><li>3.3. Above 20°, values for the Q10 were less than 2. Below 15° they were greater than 2 and increased progressively, with decreasing temperature, to values as high as 9.</li><li>4.4. The results, obtained with a purely photosynthetic system, support earlier work with intact organisms which led some investigators to conclude that photosynthesis did not obey the Arrhenius law. It is suggested that this may be a consequence of the cyclic nature of the reactions involved.</li></ul></div>","PeriodicalId":100158,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","volume":"126 2","pages":"Pages 207-213"},"PeriodicalIF":0.0,"publicationDate":"1966-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6585(66)90056-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91681144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

Studies on a barley mutant lacking chlorophyl b. I. Photochemical activity of isolated chloroplasts 大麦缺叶绿素突变体的研究。ⅰ。离体叶绿体的光化学活性

Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-10-10 DOI: 10.1016/0926-6585(66)90054-9

N.K. Boardman, H.K. Highkin

{"title":"Studies on a barley mutant lacking chlorophyl b. I. Photochemical activity of isolated chloroplasts","authors":"N.K. Boardman, H.K. Highkin","doi":"10.1016/0926-6585(66)90054-9","DOIUrl":"https://doi.org/10.1016/0926-6585(66)90054-9","url":null,"abstract":"<div><ul><li>1.1. Chloroplasts were isolated from the leaves of a barley mutant which lacked chlorophyll b and compared with the chloroplasts from the normal strain which had the normal content of chlorophyll a and chlorophyll b.</li><li>2.2. Hill-reaction measurements were made with either 2,3′,6-trichlorophenol-indophenol, ferricyanide or NADP+ as oxidant. At a light intensity of 40 000 lux, the mutant chloroplasts were more active per mg of total chlorophyll than the normal chloroplasts, but only slightly more active per mg of chlorophyll a. Normal chloroplasts were saturated at a light intensity of about 30 000 lux whereas the mutant chloroplasts required an intensity of nearly 60 000 lux for saturation. At low light intensities (< 15 000 lux) the mutant chloroplasts were less active than the normal chloroplasts. The mutant chloroplasts were inactivated at a greater rate if allowed to stand at 0° and they were more susceptible to inhibition by 1-(p-chlorophenyl)-3,3′-dimethylurea.</li><li>3.3. Microscopic examination under phase contrast showed no significant differences in the appearance of isolated chloroplasts from the normal and mutant plants.The lack of chlorophyll b in the mutant was confirmed by absorption and spectrofluorimetric measurements at the temperature of liquid nitrogen. The spectrofluorimetric method was capable of detecting one molecule of chlorophyll b in the presence of 1000 molecules of chlorophyll a.</li><li>4.4. The results are discussed in relation to the function of chlorophyll b in photosynthesis.</li></ul></div>","PeriodicalId":100158,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","volume":"126 2","pages":"Pages 189-199"},"PeriodicalIF":0.0,"publicationDate":"1966-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6585(66)90054-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91681145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 105

Measurements of reduced pyridine nucleotides in a single neuron 单个神经元中还原吡啶核苷酸的测量

Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-10-10 DOI: 10.1016/0926-6585(66)90073-2

C.A. Terzuolo, B. Chance, E. Handelman, L. Rossini, P. Schmelzer

引用次数: 25

Hydrogen-deuterium exchange in heavy meromyosin 重质肌球蛋白中的氢-氘交换

Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-10-10 DOI: 10.1016/0926-6585(66)90075-6

Lars Willumsen

引用次数: 19

Cation accumulation by microsomal (Na+ + K+)-activated ATPase 微粒体(Na+ + K+)激活的atp酶的阳离子积累

Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-10-10 DOI: 10.1016/0926-6585(66)90072-0

J.S. Charnock, L.J. Opit, J.R. Casely-Smith

{"title":"Cation accumulation by microsomal (Na+ + K+)-activated ATPase","authors":"J.S. Charnock, L.J. Opit, J.R. Casely-Smith","doi":"10.1016/0926-6585(66)90072-0","DOIUrl":"10.1016/0926-6585(66)90072-0","url":null,"abstract":"<div><ul><li>1.1. Possible cation accumulation by microsomal (Na+ + K+)-activated, ouabain-sensitive ATPase was examined by an electron microscopical method.</li><li>2.2. Densitometry measurements of membrane opacity were performed on the electron micrograph plates obtained after incubation of the ATPase preparation under a variety of experimental conditions.</li><li>3.3. To aid visualization of possible cation accumulation, the more electron opaque Cs+ was substituted for K+ in the incubation system of many experiments.</li><li>4.4. Increase in membrane density of the microsomal ATPase was observed in the presence of sodium and caesium only when ATP hydrolysis was inhibited by ouabain.</li><li>5.5. This phenomenon was not observed when a ouabain-insensitive mitochondrial ATPase system was examined.</li><li>6.6. This increase in density is interpreted as selective Cs+ accumulation by the microsomal enzyme membrane fragments.</li><li>7.7. The significance of these results is discussed in terms of reaction mechanism for (Na+ + K+)-activated ATPase.</li></ul></div>","PeriodicalId":100158,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","volume":"126 2","pages":"Pages 350-360"},"PeriodicalIF":0.0,"publicationDate":"1966-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6585(66)90072-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15336349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The optical and magnetic properties of copper in Chenopodium album plastocyanin Chenopodium质体青素中铜的光学和磁性能

Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-10-10 DOI: 10.1016/0926-6585(66)90063-X

W.E. Blumberg, J. Peisach

引用次数: 51

Photoreduction of quinones by isolated spinach chloroplasts 菠菜叶绿体光还原醌类

Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-10-10 DOI: 10.1016/0926-6585(66)90055-0

Duck H. Cho, L. Parks , Gunter Zweig

引用次数: 9