转铁蛋白在阴离子存在下的超离心行为

Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-10-10 DOI:10.1016/0926-6585(66)90065-3

Anatoly Bezkorovainy

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引用次数: 3

摘要

在BrO3−、IO3−、SCN−、Cl−、Br−、I−和NO3−存在的情况下，混合的人血浆中的转铁蛋白可逆聚集，使体重平均沉降系数从5.50 S到6.83 S，而在7%浓度下，正常的s20w为5.10 S。这种聚集在转铁蛋白等电点附近最为明显，即pH值为5.0时，这种聚集与阴离子有关，而与蛋白质浓度无关。在任何pH值下，醋酸盐、甲酸盐、氯乙酸盐、氟化物、羧酸盐和硫酸盐均未观察到聚集现象。

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Ultracentrifugal behavior of transferrin in the presence of some anions

Transferrin of pooled human plasma has been found to aggregate reversibly in the presence of BrO₃⁻, IO₃⁻, SCN⁻, Cl⁻, Br⁻, I⁻, and NO₃⁻, giving weight-average sedimentation coefficients of from 5·50 S to 6·83 S compared to a normal s_20,w of 5·10 S at 0·7% concentration. The aggregation was most pronounced in the vicinity of the isoelectric points of transferrin, i.e. at pH 5·0, and was dependent on the anion, but not on the protein concentration. No aggregation was observed in the presence of acetate, formate, chloroacetate, fluoride, cacodylate and sulfate at any pH value.

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Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis

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