Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis最新文献

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Tryptophan residues in native and reoxidized muramidase: Luminescence properties 天然和再氧化酶中的色氨酸残基:发光性质
Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-07-13 DOI: 10.1016/0926-6585(66)90307-4
Jorge E. Churchich
{"title":"Tryptophan residues in native and reoxidized muramidase: Luminescence properties","authors":"Jorge E. Churchich","doi":"10.1016/0926-6585(66)90307-4","DOIUrl":"10.1016/0926-6585(66)90307-4","url":null,"abstract":"<div><p>The luminiscence properties of tryptophan chromophores in muramidase are affected by changes in enzyme conformation.</p><p>Fluorimetric measurements indicated that the emission spectrum of native muramidase (<em>λ</em><sub>max.</sub> = 337 m<em>μ</em>) was shifted toward longer wavelengths (<em>λ</em><sub>max.</sub> = 350 m<em>μ</em>) as a result of structural changes produced by disruption and carboxymethylation of disulfide bonds. When the disulfide bonds were reformed at pH 8, the emission spectra of reoxidized muramidase resemble that of native muramidase with regard to both general structure and band position.</p><p>The phosphorescence spectra of the muramidase species revealed the predominant contribution of tryptophan chromophores to the long-lived emission, and this observation was substantiated by decay-time measurements.</p><p>The <em>τ</em><sub>p</sub> values of native and reoxidized muramidases (<em>τ</em><sub>p</sub> = 1−1.9 sec) were remarkably shorter than the <em>τ</em><sub>p</sub> value of reduced carboxymethylated muramidase (<em>τ</em><sub>p</sub> = 4.1 sec). The possible significance of these phosphorescence and fluorescence studies are discussed.</p></div>","PeriodicalId":100158,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","volume":"120 3","pages":"Pages 406-412"},"PeriodicalIF":0.0,"publicationDate":"1966-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6585(66)90307-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17038032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 27
Low-temperature action spectra for transformations of photoperiodic pigments 光周期颜料转化的低温作用光谱
Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-07-13 DOI: 10.1016/0926-6585(66)90312-8
C.J.P. Spruit
{"title":"Low-temperature action spectra for transformations of photoperiodic pigments","authors":"C.J.P. Spruit","doi":"10.1016/0926-6585(66)90312-8","DOIUrl":"10.1016/0926-6585(66)90312-8","url":null,"abstract":"","PeriodicalId":100158,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","volume":"120 3","pages":"Pages 454-456"},"PeriodicalIF":0.0,"publicationDate":"1966-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6585(66)90312-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17038036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
The stability of the two-bonded collagen triple helix 二键胶原蛋白三螺旋结构的稳定性
Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-07-13 DOI: 10.1016/0926-6585(66)90313-X
G.N. Ramachandran, C.M. Venkatachalam
{"title":"The stability of the two-bonded collagen triple helix","authors":"G.N. Ramachandran,&nbsp;C.M. Venkatachalam","doi":"10.1016/0926-6585(66)90313-X","DOIUrl":"10.1016/0926-6585(66)90313-X","url":null,"abstract":"","PeriodicalId":100158,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","volume":"120 3","pages":"Pages 457-458"},"PeriodicalIF":0.0,"publicationDate":"1966-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6585(66)90313-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17038037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Chloroplast reactions with dipyridyl salts 叶绿体与二吡啶盐的反应
Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-07-13 DOI: 10.1016/0926-6585(66)90300-1
Clanton C. Black Jr.
{"title":"Chloroplast reactions with dipyridyl salts","authors":"Clanton C. Black Jr.","doi":"10.1016/0926-6585(66)90300-1","DOIUrl":"10.1016/0926-6585(66)90300-1","url":null,"abstract":"<div><p>Illuminated spinach chloroplasts reduce quarternary dipyridyl salts with oxidation-reduction potentials ranging from −342 to −656 mV. The accumulation of reduced dipyridyl was dependent upon the oxidation-reduction potential of each dipyridyl, ranging from 100% with the 1,1′-ethylene-2,2′-dipyridylium dibromide (<em>E</em><sub>0</sub>′ = −342 mV) to 3% with the 1,1′-trimethylene-2,2′-dipyridylium-4,4′-methyl dibromide (<em>E</em><sub>0</sub>′ = −656 mV). The data suggest that the oxidation-reduction potential of a natural photosynthetic reductant(s) may be near −521 mV. Photosynthetic phosphorylation occurs concurrent with reduction under both argon and air. In the presence of oxygen the reduced dipyridyls are oxidized and H<sub>2</sub>O<sub>2</sub> is produced. The dipyridyls could be substituted for spinach ferredoxin in a dark reduction of cytochrome <em>c</em> in the presence of NADPH and NADP<sup>+</sup> reductase (EC 1.6.99.4). The polagraphic half-wave oxidation-reduction potential of the dipyridyls is reported.</p></div>","PeriodicalId":100158,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","volume":"120 3","pages":"Pages 332-340"},"PeriodicalIF":0.0,"publicationDate":"1966-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6585(66)90300-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15484980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 73
The relationship between the transport of glucose and cations across cell membranes in isolated tissues II. Effects of K+-free medium, ouabain and insulin upon the fate of glucose in rat diaphragm 离体组织中葡萄糖和阳离子跨细胞膜转运的关系[j]。无K+培养基、乌沙因和胰岛素对大鼠膈肌葡萄糖命运的影响
Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-07-13 DOI: 10.1016/0926-6585(66)90303-7
Torben Clausen
{"title":"The relationship between the transport of glucose and cations across cell membranes in isolated tissues II. Effects of K+-free medium, ouabain and insulin upon the fate of glucose in rat diaphragm","authors":"Torben Clausen","doi":"10.1016/0926-6585(66)90303-7","DOIUrl":"10.1016/0926-6585(66)90303-7","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. Incubation of isolated rat hemidiaphragm in a K<sup>+</sup>-free bicarbonate buffer has been shown to induce stimulation of glycogen synthesis and inhibition of lactate production, without change in glucose uptake.</p></span></li><li><span>2.</span><span><p>2. Essentially similar changes were produced when ouabain was added to bicarbonate buffer with a normal K<sup>+</sup> content.</p></span></li><li><span>3.</span><span><p>3. These metabolic changes were therefore considered as secondary to a decrease in active cation transport.</p></span></li><li><span>4.</span><span><p>4. Insulin (0.1 I.U./ml) was shown to stimulate glycogen synthesis, lactate production and glucose uptake.</p></span></li><li><span>5.</span><span><p>5. In the presence of insulin (0.1 I.U./ml), the addition of ouabain gave a further stimulation of glycogen synthesis. This was accompanied by a decrease in lactate production and a slight depression of the glucose uptake.</p></span></li><li><span>6.</span><span><p>6. Furthermore, it was shown that ouabain in concentrations down to 1.4·10<sup>−6</sup> M induced an inhibition of CO<sub>2</sub> production from glucose.</p></span></li></ul></div>","PeriodicalId":100158,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","volume":"120 3","pages":"Pages 361-368"},"PeriodicalIF":0.0,"publicationDate":"1966-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6585(66)90303-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17039000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 75
The formation of unpaired electrons on sulphur atoms in irradiated dry proteins as studied by electron spin resonance 用电子自旋共振研究辐照干蛋白中硫原子上不成对电子的形成
Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-07-13 DOI: 10.1016/0926-6585(66)90308-6
M.G. Ormerod, B.B. Singh
{"title":"The formation of unpaired electrons on sulphur atoms in irradiated dry proteins as studied by electron spin resonance","authors":"M.G. Ormerod,&nbsp;B.B. Singh","doi":"10.1016/0926-6585(66)90308-6","DOIUrl":"10.1016/0926-6585(66)90308-6","url":null,"abstract":"<div><p>From a study of proteins containing cystine residues, it is concluded that during high-energy irradiation electrons are trapped on the cystine residues. The resultant cystine anion reacts to give the -CH<sub>2</sub>S<sup>•</sup> radical previously observed by other workers. These reactions are affected by freeze-drying the proteins from solutions of different pH's and can be prevented by additives which scavenge electrons. If the protein contains cysteine residues, -CH<sub>2</sub>S<sup>•</sup> radicals are formed also by the migration of radicals on to the sulphydryl group of the cysteine. These results have been supported by a study of the radical reactions in irradiated oxidised and reduced glutathione.</p></div>","PeriodicalId":100158,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","volume":"120 3","pages":"Pages 413-426"},"PeriodicalIF":0.0,"publicationDate":"1966-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6585(66)90308-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15398640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
Amino acid transport by the small intestine of the rat on the counterflow phenomenon as a cause of the accelerating effect of leucine on the transintestinal transport of diamino acids 氨基酸在大鼠小肠转运中的逆流现象是亮氨酸对二氨基酸跨肠转运的加速作用的一个原因
Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-06-08 DOI: 10.1016/0926-6585(66)90348-7
B.G. Munck
{"title":"Amino acid transport by the small intestine of the rat on the counterflow phenomenon as a cause of the accelerating effect of leucine on the transintestinal transport of diamino acids","authors":"B.G. Munck","doi":"10.1016/0926-6585(66)90348-7","DOIUrl":"10.1016/0926-6585(66)90348-7","url":null,"abstract":"<div><p>The accelerating effect of leucine on the transintestinal transport of lysine has been studied.</p><p>The relationship of the transport of lysine to that of leucine was such as would be expected if the accelerating effect of leucine was a result of the combined function of the transport mechanisms of the neutral and the diamino acids. Of the two transport mechanisms that of the neutral amino acids created and maintained, on the serosal side of the transport mechanisms, a concentration of leucine sufficient to exert a counterflow effect on the transport of lysine by the transport mechanism of the diamino acids.</p><p>The transintestinal transport of arginine and ornithine was accelerated by leucine to the same extent as that of lysine.</p></div>","PeriodicalId":100158,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","volume":"120 2","pages":"Pages 282-291"},"PeriodicalIF":0.0,"publicationDate":"1966-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6585(66)90348-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17032587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 33
A nuclear magnetic resonance study and proton exchange of poly-l-alanine 聚丙氨酸的核磁共振研究及质子交换
Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-06-08 DOI: 10.1016/0926-6585(66)90351-7
R.E. Glick, L. Mandelkern, W.E. Stewart
{"title":"A nuclear magnetic resonance study and proton exchange of poly-l-alanine","authors":"R.E. Glick,&nbsp;L. Mandelkern,&nbsp;W.E. Stewart","doi":"10.1016/0926-6585(66)90351-7","DOIUrl":"10.1016/0926-6585(66)90351-7","url":null,"abstract":"","PeriodicalId":100158,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","volume":"120 2","pages":"Pages 302-304"},"PeriodicalIF":0.0,"publicationDate":"1966-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6585(66)90351-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17032590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
The fluorescence emission spectra of chlorophyll a forms from euglena 叶绿素a的荧光发射光谱形成于绿藻
Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-06-08 DOI: 10.1016/0926-6585(66)90352-9
Jeanette S. Brown
{"title":"The fluorescence emission spectra of chlorophyll a forms from euglena","authors":"Jeanette S. Brown","doi":"10.1016/0926-6585(66)90352-9","DOIUrl":"10.1016/0926-6585(66)90352-9","url":null,"abstract":"","PeriodicalId":100158,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","volume":"120 2","pages":"Pages 305-307"},"PeriodicalIF":0.0,"publicationDate":"1966-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6585(66)90352-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17032591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
The metabolism of glycine and glycollate by pea leaves in relation to photosynthesis 豌豆叶片对甘氨酸和甘氨酸的代谢与光合作用的关系
Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-06-08 DOI: 10.1016/0926-6585(66)90346-3
B.J. Miflin , A.F.H. Marker, C.P. Whittingham
{"title":"The metabolism of glycine and glycollate by pea leaves in relation to photosynthesis","authors":"B.J. Miflin ,&nbsp;A.F.H. Marker,&nbsp;C.P. Whittingham","doi":"10.1016/0926-6585(66)90346-3","DOIUrl":"10.1016/0926-6585(66)90346-3","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. The metabolism of <sup>14</sup>C-labelled glycollate and glycine both to sucrose and to a polyglucan <em>via</em> serine has been demonstrated in excised pea leaves. The effect of light, DCMU, isonicotinyl hydrazide and the partial pressure of CO<sub>2</sub> on the pathway has been studied.</p></span></li><li><span>2.</span><span><p>2. The metabolism of [<sup>14</sup>C]glycine and serine formed during a preceding period of photosynthesis has been followed during a subsequent period of photosynthesis in <sup>12</sup>CO<sub>2</sub>. The radioactivity in the glycine and serine declined whilst that in sucrose rose, suggesting that in photosynthesis some sucrose is formed <em>via</em> glycine and serine. The effect of changes in partial pressure of carbon dioxide indicates that the photosynthetic pathway may be spatially separated from that which metabolises exogenous glycine and glycollate.</p></span></li></ul></div>","PeriodicalId":100158,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","volume":"120 2","pages":"Pages 266-273"},"PeriodicalIF":0.0,"publicationDate":"1966-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6585(66)90346-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17032586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 39
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