Cation accumulation by microsomal (Na+ + K+)-activated ATPase

Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-10-10 DOI:10.1016/0926-6585(66)90072-0

J.S. Charnock, L.J. Opit, J.R. Casely-Smith

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引用次数: 2

Abstract

1.
1. Possible cation accumulation by microsomal (Na⁺ + K⁺)-activated, ouabain-sensitive ATPase was examined by an electron microscopical method.
2.
2. Densitometry measurements of membrane opacity were performed on the electron micrograph plates obtained after incubation of the ATPase preparation under a variety of experimental conditions.
3.
3. To aid visualization of possible cation accumulation, the more electron opaque Cs⁺ was substituted for K⁺ in the incubation system of many experiments.
4.
4. Increase in membrane density of the microsomal ATPase was observed in the presence of sodium and caesium only when ATP hydrolysis was inhibited by ouabain.
5.
5. This phenomenon was not observed when a ouabain-insensitive mitochondrial ATPase system was examined.
6.
6. This increase in density is interpreted as selective Cs⁺ accumulation by the microsomal enzyme membrane fragments.
7.
7. The significance of these results is discussed in terms of reaction mechanism for (Na⁺ + K⁺)-activated ATPase.

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微粒体(Na+ + K+)激活的atp酶的阳离子积累

1.1. 用电镜方法检测了微粒体(Na+ + K+)激活的对瓦阿拜因敏感的atp酶可能的阳离子积累。在各种实验条件下，对atp酶制剂孵育后获得的电子显微镜板进行膜不透明度的密度测量。为了帮助可视化可能的阳离子积累，在许多实验的培养体系中，用电子不透明的Cs+代替K+。在钠和铯存在的情况下，只有当ATP水解被瓦阿因抑制时，微粒体ATP酶的膜密度才会增加。6.6.当检测瓦巴因不敏感的线粒体atp酶系统时，没有观察到这种现象。这种密度的增加被解释为微粒体酶膜碎片选择性的Cs+积累。从(Na+ + K+)活化atp酶的反应机制方面讨论了这些结果的意义。

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Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis

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