{"title":"微粒体(Na+ + K+)激活的atp酶的阳离子积累","authors":"J.S. Charnock, L.J. Opit, J.R. Casely-Smith","doi":"10.1016/0926-6585(66)90072-0","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. Possible cation accumulation by microsomal (Na<sup>+</sup> + K<sup>+</sup>)-activated, ouabain-sensitive ATPase was examined by an electron microscopical method.</p></span></li><li><span>2.</span><span><p>2. Densitometry measurements of membrane opacity were performed on the electron micrograph plates obtained after incubation of the ATPase preparation under a variety of experimental conditions.</p></span></li><li><span>3.</span><span><p>3. To aid visualization of possible cation accumulation, the more electron opaque Cs<sup>+</sup> was substituted for K<sup>+</sup> in the incubation system of many experiments.</p></span></li><li><span>4.</span><span><p>4. Increase in membrane density of the microsomal ATPase was observed in the presence of sodium and caesium only when ATP hydrolysis was inhibited by ouabain.</p></span></li><li><span>5.</span><span><p>5. This phenomenon was not observed when a ouabain-insensitive mitochondrial ATPase system was examined.</p></span></li><li><span>6.</span><span><p>6. This increase in density is interpreted as selective Cs<sup>+</sup> accumulation by the microsomal enzyme membrane fragments.</p></span></li><li><span>7.</span><span><p>7. The significance of these results is discussed in terms of reaction mechanism for (Na<sup>+</sup> + K<sup>+</sup>)-activated ATPase.</p></span></li></ul></div>","PeriodicalId":100158,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","volume":"126 2","pages":"Pages 350-360"},"PeriodicalIF":0.0000,"publicationDate":"1966-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6585(66)90072-0","citationCount":"2","resultStr":"{\"title\":\"Cation accumulation by microsomal (Na+ + K+)-activated ATPase\",\"authors\":\"J.S. Charnock, L.J. Opit, J.R. Casely-Smith\",\"doi\":\"10.1016/0926-6585(66)90072-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. Possible cation accumulation by microsomal (Na<sup>+</sup> + K<sup>+</sup>)-activated, ouabain-sensitive ATPase was examined by an electron microscopical method.</p></span></li><li><span>2.</span><span><p>2. Densitometry measurements of membrane opacity were performed on the electron micrograph plates obtained after incubation of the ATPase preparation under a variety of experimental conditions.</p></span></li><li><span>3.</span><span><p>3. To aid visualization of possible cation accumulation, the more electron opaque Cs<sup>+</sup> was substituted for K<sup>+</sup> in the incubation system of many experiments.</p></span></li><li><span>4.</span><span><p>4. Increase in membrane density of the microsomal ATPase was observed in the presence of sodium and caesium only when ATP hydrolysis was inhibited by ouabain.</p></span></li><li><span>5.</span><span><p>5. This phenomenon was not observed when a ouabain-insensitive mitochondrial ATPase system was examined.</p></span></li><li><span>6.</span><span><p>6. This increase in density is interpreted as selective Cs<sup>+</sup> accumulation by the microsomal enzyme membrane fragments.</p></span></li><li><span>7.</span><span><p>7. The significance of these results is discussed in terms of reaction mechanism for (Na<sup>+</sup> + K<sup>+</sup>)-activated ATPase.</p></span></li></ul></div>\",\"PeriodicalId\":100158,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis\",\"volume\":\"126 2\",\"pages\":\"Pages 350-360\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1966-10-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6585(66)90072-0\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926658566900720\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926658566900720","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Cation accumulation by microsomal (Na+ + K+)-activated ATPase
1.
1. Possible cation accumulation by microsomal (Na+ + K+)-activated, ouabain-sensitive ATPase was examined by an electron microscopical method.
2.
2. Densitometry measurements of membrane opacity were performed on the electron micrograph plates obtained after incubation of the ATPase preparation under a variety of experimental conditions.
3.
3. To aid visualization of possible cation accumulation, the more electron opaque Cs+ was substituted for K+ in the incubation system of many experiments.
4.
4. Increase in membrane density of the microsomal ATPase was observed in the presence of sodium and caesium only when ATP hydrolysis was inhibited by ouabain.
5.
5. This phenomenon was not observed when a ouabain-insensitive mitochondrial ATPase system was examined.
6.
6. This increase in density is interpreted as selective Cs+ accumulation by the microsomal enzyme membrane fragments.
7.
7. The significance of these results is discussed in terms of reaction mechanism for (Na+ + K+)-activated ATPase.
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