Advances in Sample Preparation最新文献

{"title":"High-throughput analytical methods employing microextraction techniques: Towards fast analyses lasting a few seconds","authors":"Eduardo Carasek,&nbsp;Rafael Scur,&nbsp;Gabrieli Bernardi","doi":"10.1016/j.sampre.2023.100095","DOIUrl":"https://doi.org/10.1016/j.sampre.2023.100095","url":null,"abstract":"<div><p>Green Analytical Chemistry (GAC) principles have influenced the development of analytical methods with minimal sample handling and operations, in contrast to conventional techniques, which are often laborious and time-consuming. Since the introduction of microextraction techniques in the 1990s, various approaches, configurations, and sorptive phases have been proposed to replace Solid Phase Extraction (SPE) and Liquid-Liquid Extraction (LLE), covering a wide range of matrices and analytes, focused on chromatography and mass spectrometry instrumentation. The main features of microextraction techniques are simplicity, low solvent consumption, and minimum residue generation. The demand for fast results and the large number of samples, along with advances in analytical instrumentation, have led to the coupling of microextraction techniques with automation and/or the development of technologies for multiple extractions at the same time, known as parallel extractions. As a result of its popularity in medical and pharmaceutical sciences, the 96-well plate has been successfully adapted for Solid Phase Microextraction (SPME) and Liquid Phase Microextraction (LPME), significantly reducing the time required to process a large number of samples. This review presents some of the basic principles of microextraction techniques, and it contextualizes and compares analytical methods published in the period of 2018 to early 2023 in the microextraction context for high-throughput analyses.</p></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"8 ","pages":"Article 100095"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49750824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of high-pH fractionation and cotton-HILIC enrichment approaches for analyzing N-glycopeptides","authors":"Nabil Tahhan ,&nbsp;Sena Aksoy ,&nbsp;Haci Mehmet Kayili ,&nbsp;Bekir Salih","doi":"10.1016/j.sampre.2023.100098","DOIUrl":"https://doi.org/10.1016/j.sampre.2023.100098","url":null,"abstract":"<div><p>Glycosylation, a prevalent post-translational modification, plays a significant role in diverse biological processes and exhibits associations with various diseases, including cancer. Among the different types of glycosylation, <em>N-</em>glycosylation is particularly prominent. Glycosylation analysis also plays a crucial role in understanding the efficacy of protein biopharmaceuticals, as the majority of these drugs are glycoproteins. The precise detection and characterization of <em>N-</em>glycosylation require a series of enrichment steps. On the other hand, peptide fragmentation methods constitute a crucial step in reducing sample complexity. Moreover, enrichment strategies coupled with mass spectrometry are essential for the analysis and identification of <em>N-</em>glycopeptides. This research aims to enhance the detection methods for <em>N-</em>glycopeptides and <em>N-</em>glycoproteins by employing strategies that involve peptide fractionation and glycopeptide enrichment. This study targeted to assess and compare the efficacy of an integration method and a direct enrichment approach in terms of identifying glycopeptides from the glycoproteome of human plasma. The results demonstrate that the integration method detects 212 <em>N-</em>glycopeptides and 88 <em>N-</em>glycoproteins, while Cotton-HILIC direct enrichment detects 88 <em>N-</em>glycopeptides and 41 <em>N-</em>glycoproteins. In conclusion, the employment of Cotton-HILIC enrichment with high pH fractionation exhibits greater qualitative abilities in characterizing the structures and functions of glycopeptides.</p></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"8 ","pages":"Article 100098"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772582023000487/pdfft?md5=317f642a6f6fef3c90ba327cea2fc9bb&pid=1-s2.0-S2772582023000487-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92066683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient analysis of polycyclic aromatic hydrocarbons by dispersive-µ-solid-phase extraction using silica-based nanostructured sorbent phases coupled to gas chromatography-mass spectrometry","authors":"Camila Scheid ,&nbsp;Wendell Mello ,&nbsp;Silvio Buchner ,&nbsp;Edilson Valmir Benvenutti ,&nbsp;Monique Deon ,&nbsp;Josias Merib","doi":"10.1016/j.sampre.2023.100070","DOIUrl":"https://doi.org/10.1016/j.sampre.2023.100070","url":null,"abstract":"<div><p>In this study, SBA-15 mesoporous silica has been investigated as sorbent phase for the extraction and determination of 10 polycyclic aromatic hydrocarbons (PAHs) in environmental water samples for the first time. The extraction step was performed through a dispersive-µ-solid-phase extraction (D-µ-SPE) followed by gas chromatography coupled to mass spectrometry (GC-MS). In particular, the surface of SBA-15 was chemically bonded to different functional groups (aminopropyl, phenyl, octyl and octadecyl), and the extraction efficiency of each sorbent phase was investigated. The sorbent phase was synthesized and characterized by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), N₂ sorption analysis (Brunauer–Emmett–Teller), and thermogravimetric analysis (TGA). The optimized extraction conditions consisted of using 2 mg of the sorbent SBA-15/C8, 2 mL of sample, 2 min of vortex, 4 min of stirring in orbital shaker and 1 min of centrifugation; the desorption step was performed with 25 µL of ACN:acetone (50:50 v/v), vortex for 2 min, 8 min of stirring in orbital shaker and 3 min of centrifugation. The proposed method was validated, with determination coefficients higher than 0.99 for all analytes; LODs and LOQs ranged from 0.15 to 3.03 μg L⁻¹ and from 0.5 to 10.0 μg L⁻¹, respectively; intraday precision ranged from 2.6 to 12.1%; interday precision varied from 4.5 to 25.3%; and accuracy ranged from 92.0 to 112.3 %. This methodology was successfully applied for the analysis of eight groundwater samples from monitoring wells of gas stations in São Paulo. Moreover, according to AGREEprep metrics, the proposed method can be considered sustainable according to the concepts of green analytical chemistry.</p></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"7 ","pages":"Article 100070"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49750627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metal organic frameworks as advanced materials for the paper-based extraction of synthetic cannabinoids in oral fluids","authors":"Jens van der Hulst,&nbsp;Héctor Martínez-Pérez-Cejuela,&nbsp;José Manuel Herrero-Martínez,&nbsp;Sergio Armenta","doi":"10.1016/j.sampre.2023.100077","DOIUrl":"https://doi.org/10.1016/j.sampre.2023.100077","url":null,"abstract":"<div><p>In this manuscript, a paper-based device modified with metal-organic frameworks was applied for the extraction of synthetic cannabinoid receptor agonists (SCRAs) in oral fluids, taking profit of the several interaction mechanisms between MOF-analytes, followed by their quantification by LC-fluorescence detection. The resulting composite, named as NH₂-UiO-66@paper, was characterized by different techniques such as infrared, scanning electron microscopy, among others. The extraction procedure was optimized by using a full factorial design after an appropriate selection of the significant variables. Once the method was optimized, the analytical parameters were studied including liner range (LOQ-400 µg L⁻¹), limit of detection (0.7 to 2.8 µg L⁻¹) and intra- and inter-batch precision (&lt;8 and &lt;20%, expressed as RSD, respectively). Next, the accuracy of the method was assessed at three different concentration levels (25, 75 and 100 µg L⁻¹), obtaining values comprised from 64 to 98%. Finally, the method was applied for the analysis of oral fluids (n=25) (15 positives and 10 negative samples) and sensitivity, specificity and accuracy (efficiency) of the methodology were calculated obtaining 80, 100 and 88%, respectively.</p></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"7 ","pages":"Article 100077"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49750186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial activity and antibiotic-modifying action of carvacrol against multidrug-resistant bacteria","authors":"Ana Raquel Pereira da Silva ,&nbsp;Maria do Socorro Costa ,&nbsp;Nara Juliana Santos Araújo ,&nbsp;Thiago Sampaio de Freitas ,&nbsp;Antônia Thassya Lucas dos Santos ,&nbsp;Sheila Alves Gonçalves ,&nbsp;Viviane Bezerra da Silva ,&nbsp;Jacqueline Cosmo Andrade-Pinheiro ,&nbsp;Catarina Martins Tahim ,&nbsp;Elaine Cristina Pereira Lucetti ,&nbsp;Henrique Douglas Melo Coutinho","doi":"10.1016/j.sampre.2023.100072","DOIUrl":"https://doi.org/10.1016/j.sampre.2023.100072","url":null,"abstract":"<div><p>Bacterial resistance is a serious health problem, which is mostly caused by the inappropriate use of antibiotics. The emergence of strains resistant to these drugs results in difficulties in the treatment of bacterial infections. Therefore, it has been a trend to investigate new alternatives against microbial resistance such as the use of natural products and their major compounds. The monoterpene carvacrol which is mostly extracted from essential oils has been pointed as a promisor. In the search for better results in terms of pharmaceutical applications of associations and complexes, β-cyclodextrin has been promising when complexed with isolated compounds. This study aimed to evaluate the antibacterial activity of the compound carvacrol, pure and complexed with β-cyclodextrin, against the strains of <em>Staphylococcus aureus, Pseudomonas aeruginosa</em>, and <em>Escherichia coli</em>. The antibacterial activity assay was performed using the broth microdilution method, determining the minimum inhibitory concentration (MIC), and from the subinhibitory concentration value (MIC/8) was evaluated the modifier activity of the antibiotic action. The reading of the tests was carried out using sodium resazurin and expressed by geometric mean. Carvacrol showed significant results against standard bacteria: <em>S. aureus</em> with a MIC of 81 μg/mL, and <em>P. aeruginosa</em> with a MIC of 161 μg/mL. Also, a MIC of 128 μg/mL for the multidrug-resistant strain of <em>P. aeruginosa</em>. Regarding the modifier effect on antibiotic action when associated with gentamicin, complexed carvacrol showed synergism against <em>E. coli</em>, with a reduction in the MIC from 25 μg/mL to 5 μg/mL. Carvacrol demonstrated relevant clinical antibacterial activity, for both gram-positive and gram-negative bacteria, and a synergistic effect when associated with gentamicin. Therefore, these results showed to be promising in fighting bacterial resistance.</p></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"7 ","pages":"Article 100072"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49750571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-line gas purging exploiting a novel converging flow-on paper optosensing device for the determination of total ammonia-nitrogen in water samples","authors":"Ali Sahragard ,&nbsp;Waleed Alahmad ,&nbsp;Thidarat Samkumpim ,&nbsp;Manuel Miró ,&nbsp;Pakorn Varanusupakul","doi":"10.1016/j.sampre.2023.100076","DOIUrl":"https://doi.org/10.1016/j.sampre.2023.100076","url":null,"abstract":"<div><p>A novel, inexpensive, and sensitive microanalytical system for the determination of total ammonia-nitrogen (ammonia-N) in water was developed. The system was designed to detect ammonia using an in-line purging system coupled with a newly designed gas converging flow cell incorporating in-line paper-based colorimetric detection. The gas converging flow cell served as a gas chamber, a paper-based device holder, and a color detection cell. The paper-based device was impregnated with butterfly pea extract (a natural acid-base indicator), the color of which changed on the basis of the generated ammonia gas. Analytical parameters such as concentration of natural indicator and purging time were studied and optimized. The color image could be captured by an integrated endoscope camera or smartphone and analyzed by ImageJ software in RGB mode. Under optimal conditions, two linear dynamic ranges were observed. By using 1:2 (v/v) diluted 2% (w/v) butterfly pea extract in Milli-Q water, a low linear concentration range of 0.05–0.9 mg L⁻¹ with a limit of detection (LOD) of 0.02 mg L⁻¹ and limit of quantification (LOQ) of 0.05 mg L⁻¹ was achieved. By using a non-diluted 2% (w/v) butterfly pea extract, a high linear concentration range of 0.5–4 mg L⁻¹ with an LOD of 0.1 mg L⁻¹ and LOQ of 0.3 mg L⁻¹ was obtained. Finally, the optimized method was leveraged to determine ammonia-N in various water samples from shrimp farming.</p></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"7 ","pages":"Article 100076"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49750835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and modification of MIL-101-NH2-based metal organic frameworks for preparation of SPME fibers using sol-gel methodology","authors":"Hasan Javanmardi ,&nbsp;Mahsa Doosti ,&nbsp;Alireza Abbasi ,&nbsp;Habib Bagheri","doi":"10.1016/j.sampre.2023.100075","DOIUrl":"https://doi.org/10.1016/j.sampre.2023.100075","url":null,"abstract":"<div><p>Implementation of metal-organic frameworks (MOFs) in the separation science is attractive for many researchers, nowadays. In this study, three MOFs were synthesized and employed as extractive coating fibers. The MIL-101-NH₂-based coatings were implemented in immersed solid phase microextraction (ISPME) of organophosphorus pesticides (OPPs) from real-life samples. The three different kinds of fiber coatings included MIL-101-NH₂ and its acyl and ethyl modified structures as particles and 3-aminopropyl triethoxysilane (APTES) sol-gel precursor as binder. Each single MOF was coated on a stainless steel substrate <em>via</em> sol-gel technique. The uniform and tunable pore structure, high surface area, and capability to functionalization of MIL-101-NH₂, make it susceptible for isolation of a wide range of analytes from small to large molecules. Extraction performance of the synthesized fiber coatings with different functionalities was examined on some model OPPs, followed by gas chromatography-mass spectrometry (GC–MS). The highest extraction efficiency was achieved by the acyl-modified (MIL-101-NH<img>COCH₃) fiber coating. The effects of various parameters affecting the extraction and desorption efficiency such as desorption time, desorption temperature, ionic strength, stirring rate, extraction time and temperature for the selected fiber were investigated and optimized. All the synthesized fiber coatings showed sufficient affinity while the acyl modified extractive phase was found to be very sensitive for determination of OPPs with reliable figures of merit such as acceptable linear dynamic ranges (1–1000 &amp; 5–1000 ng.kg⁻¹, here two linear dynamic ranges (LDR) are reported due to the variation of LDR ranges from one analyte to another), low limits of detection (0.2–1 ng.kg⁻¹), suitable intra–day (RSD% &lt;13%) and inter–day relative standard deviation (RSD% &lt;15%).</p></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"7 ","pages":"Article 100075"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49750838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality by design approach with design of experiment for sample preparation techniques","authors":"Saniye Özcan ,&nbsp;Serkan Levent ,&nbsp;Nafiz Öncü Can","doi":"10.1016/j.sampre.2023.100079","DOIUrl":"https://doi.org/10.1016/j.sampre.2023.100079","url":null,"abstract":"<div><p>The quality by design (QbD) based approach has begun to be embedded in various method development. Studies show that with the design of experiment tool, the analysis process has become more efficient, faster, and easier. The analysis has two components: Method development and sample preparation. Sample preparation can be at least as difficult as method development, and sometimes even more difficult. Therefore, in addition to the new techniques and materials developed in sample preparation, the QbD approach can significantly reduce the workload of the analysts and provide analysis with high accuracy and precision. So far, very limited studies have been come across in the literature about the QbD approach. The review in question has been prepared to encourage and guide researchers on this subject, and will greatly support this deficiency.</p></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"7 ","pages":"Article 100079"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49750191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supramolecular solvents for multi-target and matrix-independent sample preparation","authors":"Ana Ballesteros-Gómez,&nbsp;Soledad Rubio","doi":"10.1016/j.sampre.2023.100085","DOIUrl":"https://doi.org/10.1016/j.sampre.2023.100085","url":null,"abstract":"<div><p>The development of sample treatments based on supramolecular solvents (SUPRAS) has rapidly increased during the last decade. The reasons behind this interest are, among others, the need for more sustainable solvents, but above all, the demand for more efficient and fit-for-purpose sample treatments that face the current challenges confronted by analytical laboratories and some cutting-edge research areas. One of the most outstanding features of SUPRAS is their huge potential for designing ad-hoc sample treatments that solve specific analytical and operational issues. In this article, this potential is exemplified with the achievements obtained in two demanding areas: the screening/quantification of multiclass compounds and the development of matrix-independent analytical methods. We also highlight the need for development of knowledge-based tailoring of solvents that provide fit-for-purpose sample treatments in areas where current methodologies are not applicable or need better performance.</p></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"7 ","pages":"Article 100085"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49750746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green sample preparation 2023","authors":"Francisco Pena-Pereira ,&nbsp;Verónica Pino ,&nbsp;Marek Tobiszewski ,&nbsp;Lorena Vidal","doi":"10.1016/j.sampre.2023.100087","DOIUrl":"https://doi.org/10.1016/j.sampre.2023.100087","url":null,"abstract":"","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"7 ","pages":"Article 100087"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49759347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}