Chinese Journal of Natural Medicines最新文献

{"title":"Notoginsenoside Ft1 inhibits colorectal cancer growth by increasing CD8+ T cell proportion in tumor-bearing mice through the USP9X signaling pathway","authors":"Yutao FENG ,&nbsp;Yuan LI ,&nbsp;Fen MA ,&nbsp;Enjiang WU ,&nbsp;Zewei CHENG ,&nbsp;Shiling ZHOU ,&nbsp;Zhengtao WANG ,&nbsp;Li YANG ,&nbsp;Xun SUN ,&nbsp;Jiwei ZHANG","doi":"10.1016/S1875-5364(24)60623-0","DOIUrl":"https://doi.org/10.1016/S1875-5364(24)60623-0","url":null,"abstract":"<div><p>The management of colorectal cancer (CRC) poses a significant challenge, necessitating the development of innovative and effective therapeutics. Our research has shown that notoginsenoside Ft1 (Ng-Ft1), a small molecule, markedly inhibits subcutaneous tumor formation in CRC and enhances the proportion of CD8⁺ T cells in tumor-bearing mice, thus restraining tumor growth. Investigation into the mechanism revealed that Ng-Ft1 selectively targets the deubiquitination enzyme USP9X, undermining its role in shielding <em>β</em>-catenin. This leads to a reduction in the expression of downstream effectors in the Wnt signaling pathway. These findings indicate that Ng-Ft1 could be a promising small-molecule treatment for CRC, working by blocking tumor progression <em>via</em> the Wnt signaling pathway and augmenting CD8⁺ T cell prevalence within the tumor environment.</p></div>","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140631281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carrimycin ameliorates lipopolysaccharide and cecal ligation and puncture-induced sepsis in mice","authors":"Junzhong LAI ,&nbsp;Jiadi LIANG ,&nbsp;Kunsen CHEN ,&nbsp;Biyun GUAN ,&nbsp;Zhirong CHEN ,&nbsp;Linqin CHEN ,&nbsp;Jiqiang FAN ,&nbsp;Yong ZHANG ,&nbsp;Qiumei LI ,&nbsp;Jingqian SU ,&nbsp;Qi CHEN ,&nbsp;Jizhen LIN","doi":"10.1016/S1875-5364(24)60600-X","DOIUrl":"https://doi.org/10.1016/S1875-5364(24)60600-X","url":null,"abstract":"<div><p>Carrimycin (CA), sanctioned by China's National Medical Products Administration (NMPA) in 2019 for treating acute bronchitis and sinusitis, has recently been observed to exhibit multifaceted biological activities, encompassing anti-inflammatory, antiviral, and anti-tumor properties. Despite these applications, its efficacy in sepsis treatment remains unexplored. This study introduces a novel function of CA, demonstrating its capacity to mitigate sepsis induced by lipopolysaccharide (LPS) and cecal ligation and puncture (CLP) in mice models. Our research employed <em>in vitro</em> assays, real-time quantitative polymerase chain reaction (RT-qPCR), and RNA-seq analysis to establish that CA significantly reduces the levels of pro-inflammatory cytokines, namely tumor necrosis factor-alpha (TNF-α), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6), in response to LPS stimulation. Additionally, Western blotting and immunofluorescence assays revealed that CA impedes Nuclear Factor Kappa B (NF-<em>κ</em>B) activation in LPS-stimulated RAW264.7 cells. Complementing these findings, <em>in vivo</em> experiments demonstrated that CA effectively alleviates LPS- and CLP-triggered organ inflammation in C57BL/6 mice. Further insights were gained through 16S sequencing, highlighting CA's pivotal role in enhancing gut microbiota diversity and modulating metabolic pathways, particularly by augmenting the production of short-chain fatty acids in mice subjected to CLP. Notably, a comparative analysis revealed that CA's anti-inflammatory efficacy surpasses that of equivalent doses of aspirin (ASP) and TIENAM. Collectively, these findings suggest that CA exhibits significant therapeutic potential in sepsis treatment. This discovery provides a foundational theoretical basis for the clinical application of CA in sepsis management.</p></div>","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140309713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and microbial transformation of tea sapogenin from seed pomace of Camellia oleifera with anti-inflammatory effects","authors":"Pingping SHEN ,&nbsp;Xuewa JIANG ,&nbsp;Jingling ZHANG ,&nbsp;Jiayi WANG ,&nbsp;Richa Raj ,&nbsp;Guolong LI ,&nbsp;Haixia GE ,&nbsp;Weiwei WANG ,&nbsp;Boyang YU ,&nbsp;Jian ZHANG","doi":"10.1016/S1875-5364(24)60598-4","DOIUrl":"https://doi.org/10.1016/S1875-5364(24)60598-4","url":null,"abstract":"<div><p>In the current study, tea saponin, identified as the primary bioactive constituent in seed pomace of <em>Camellia oleifera</em> Abel., was meticulously extracted and hydrolyzed to yield five known sapogenins: 16-<em>O</em>-tiglogycamelliagnin B (<strong>a</strong>), camelliagnin A (<strong>b</strong>), 16-<em>O</em>-angeloybarringtogenol C (<strong>c</strong>), theasapogenol E (<strong>d</strong>), theasapogenol F (<strong>e</strong>). Subsequent biotransformation of compound <strong>a</strong> facilitated the isolation of six novel metabolites (<strong>a1</strong>−<strong>a6</strong>). The anti-inflammatory potential of these compounds was assessed using pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns molecules (DAMPs)-mediated cellular inflammation models. Notably, compounds <strong>b</strong> and <strong>a2</strong> demonstrated significant inhibitory effects on both lipopolysaccharide (LPS) and high-mobility group box 1 (HMGB1)-induced inflammation, surpassing the efficacy of the standard anti-inflammatory agent, carbenoxolone. Conversely, compounds <strong>d</strong>, <strong>a3</strong>, and <strong>a6</strong> selectivity targeted endogenous HMGB1-induced inflammation, showcasing a pronounced specificity. These results underscore the therapeutic promise of <em>C. oleifera</em> seed pomace-derived compounds as potent agents for the management of inflammatory diseases triggered by infections and tissue damage.</p></div>","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140309084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three rare anti-inflammatory sesquiterpene lactones from Magnolia grandiflora","authors":"Shuangyu XU ,&nbsp;Feng ZHANG ,&nbsp;Linlan TAO ,&nbsp;Yangming JIANG ,&nbsp;Tao HUANG ,&nbsp;Yanan LI ,&nbsp;Zhanxing HU ,&nbsp;Jue YANG ,&nbsp;Xiaojiang HAO ,&nbsp;Chunmao YUAN","doi":"10.1016/S1875-5364(24)60601-1","DOIUrl":"https://doi.org/10.1016/S1875-5364(24)60601-1","url":null,"abstract":"<div><p>Four new sesquiterpene lactones (SLs) (<strong>1</strong>–<strong>4</strong>), along with a biosynthetically related SL (<strong>5</strong>), have been isolated from the leaves of <em>Magnolia grandiflora</em>. Magrandate A (<strong>1</strong>) is notable as the first C18 homogemarane type SL, featuring a unique 1,7-dioxaspiro[4.4]nonan-6-one core. Compounds <strong>2</strong> and <strong>3</strong>, representing the first instances of chlorine-substituted gemarane-type SL analogs in natural products, were also identified. The structures of these isolates were elucidated through a combination of spectroscopic data analysis, electronic circular dichroism calculations, and X-ray single-crystal diffraction analysis. All isolates demonstrated anti-inflammatory activity in lipopolysaccharide-stimulated RAW264.7 cells. Notably, <strong>3</strong>–<strong>5</strong> showed a significant inhibitory effect on nitric oxide production, with IC₅₀ values ranging from 0.79 to 4.73 μmol·L⁻¹. Additionally, <strong>4</strong> and <strong>5</strong> exhibited moderate cytotoxic activities against three cancer cell lines, with IC₅₀ values between 3.09 and 11.23 μmol·L⁻¹.</p></div>","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140309082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethanol extract of Cyathulae Radix inhibits osteoclast differentiation and bone loss","authors":"Liying SHI ,&nbsp;Liuyi REN ,&nbsp;Jinping LI ,&nbsp;Xin LIU ,&nbsp;Jingjing LU ,&nbsp;Lujuan JIA ,&nbsp;Baoping XIE ,&nbsp;Siyuan TANG ,&nbsp;Wei LIU ,&nbsp;Jie ZHANG","doi":"10.1016/S1875-5364(24)60596-0","DOIUrl":"https://doi.org/10.1016/S1875-5364(24)60596-0","url":null,"abstract":"<div><p>Cyathulae Radix, a traditional Chinese medicine and a common vegetable, boasts a history spanning millennia. It enhances bone density, boosts metabolism, and effectively alleviates osteoporosis-induced pain. Despite its historical use, the molecular mechanisms behind Cyathulae Radix's impact on osteoporosis remain unexplored. In this study, we investigated the effects and mechanisms of Cyathulae Radix ethanol extract (CEE) in inhibiting osteoporosis and osteoclastogenesis. Eight-week-old female mice underwent ovariectomy and were treated with CEE for eight weeks. Micro-computed tomography (micro-CT) assessed histomorphometric parameters, bone tissue staining observed distal femur histomorphology, and three-point bending tests evaluated tibia mechanical properties. Enzyme-linked immunosorbent assay (ELISA) measured serum estradiol (E₂), receptor activator for nuclear factor B ligand (RANKL), and osteoprotegerin (OPG) levels. Osteoclastogenesis-related markers were analyzed <em>via</em> Western blotting (WB) and quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, CEE effects on RANKL-induced osteoclast formation and bone resorption were investigated in vitro using tartrate-resistant acid phosphatase (TRAP) staining, qRT-PCR, and WB assay. Compared with the ovariectomy (OVX) group, CEE treatment enhanced trabecular bone density, maximal load-bearing capacity, and various histomorphometric parameters. Serum E₂ and OPG levels significantly increased, while Receptor activator of nuclear factor-<em>κ</em>B (RANK) decreased in the CEE group. CEE downregulated matrix metallopeptidase 9 (MMP-9), Cathepsin K (CTSK), and TRAP gene and protein expression. In bone marrow macrophages (BMMs), CEE reduced mature osteoclasts, bone resorption pit areas, and MMP-9, CTSK, and TRAP expression during osteoclast differentiation. Compared with DMSO treatment, CEE markedly inhibited RANK, TNF receptor associated factor 6 (TRAF6), Proto-oncogene c-Fos (c-Fos), Nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) expressions, and Extracellular regulated protein kinases (ERK), c-Jun N-terminal kinase (JNK), NF-kappa B-p65 (p65) phosphorylation in osteoclasts. In conclusion, CEE significantly inhibits OVX-induced osteoporosis and RANKL-induced osteoclastogenesis, potentially through modulating the Estrogen Receptor (ER)/RANK/NFATc1 signaling pathway.</p></div>","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140309711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An inulin-type fructan CP-A from Codonopsis pilosula attenuates experimental colitis in mice by promoting autophagy-mediated inactivation of NLRP3 inflammasome","authors":"Jiangtao ZHOU ,&nbsp;Jun WANG ,&nbsp;Jiajing WANG ,&nbsp;Deyun LI,&nbsp;Jing HOU,&nbsp;Jiankuan LI,&nbsp;Yun'e BAI,&nbsp;Jianping GAO","doi":"10.1016/S1875-5364(24)60556-X","DOIUrl":"https://doi.org/10.1016/S1875-5364(24)60556-X","url":null,"abstract":"<div><p>Inulin-type fructan CP-A, a predominant polysaccharide in <em>Codonopsis pilosula</em>, demonstrates regulatory effects on immune activity and anti-inflammation. The efficacy of CP-A in treating ulcerative colitis (UC) is, however, not well-established. This study employed an <em>in vitro</em> lipopolysaccharide (LPS)-induced colonic epithelial cell model (NCM460) and an <em>in vivo</em> dextran sulfate sodium (DSS)-induced colitis mouse model to explore CP-A's protective effects against experimental colitis and its underlying mechanisms. We monitored the clinical symptoms in mice using various parameters: body weight, disease activity index (DAI), colon length, spleen weight, and histopathological scores. Additionally, molecular markers were assessed through enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qRT-PCR), immunofluorescence (IF), immunohistochemistry (IHC), and Western blotting assays. Results showed that CP-A significantly reduced reactive oxygen species (ROS), tumor necrosis factor-alpha (TNF-α), and interleukins (IL-6, IL-1β, IL-18) in LPS-induced cells while increasing IL-4 and IL-10 levels and enhancing the expression of Claudin-1, ZO-1, and occludin proteins in NCM460 cells. Correspondingly, <em>in vivo</em> findings revealed that CP-A administration markedly improved DAI, reduced colon shortening, and decreased the production of myeloperoxidase (MPO), malondialdehyde (MDA), ROS, IL-1β, IL-18, and NOD-like receptor protein 3 (NLRP3) inflammasome-associated genes/proteins in UC mice. CP-A treatment also elevated glutathione (GSH) and superoxide dismutase (SOD) levels, stimulated autophagy (LC3B, P62, Beclin-1, and ATG5), and reinforced Claudin-1 and ZO-1 expression, thereby aiding in intestinal epithelial barrier repair in colitis mice. Notably, the inhibition of autophagy <em>via</em> chloroquine (CQ) diminished CP-A's protective impact against colitis <em>in vivo</em>. These findings elucidate that CP-A's therapeutic effect on experimental colitis possibly involves mitigating intestinal inflammation through autophagy-mediated NLRP3 inflammasome inactivation. Consequently, inulin-type fructan CP-A emerges as a promising drug candidate for UC treatment.</p></div>","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140309067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yanggan Jiangmei Formula alleviates hepatic inflammation and lipid accumulation in non-alcoholic steatohepatitis by inhibiting the NF-κB/NLRP3 signaling pathway","authors":"Yuanyuan WU ,&nbsp;Jingwen ZHOU ,&nbsp;Xinchen ZUO ,&nbsp;Yufeng KUANG ,&nbsp;Lixia SUN ,&nbsp;Xiaolong ZHANG","doi":"10.1016/S1875-5364(24)60595-9","DOIUrl":"https://doi.org/10.1016/S1875-5364(24)60595-9","url":null,"abstract":"<div><p>The role of NF-<em>κ</em>B and the NLRP3 inflammasome in the chronic inflammatory microenvironment of non-alcoholic steatohepatitis (NASH) has been posited as crucial. The Yanggan Jiangmei Formula (YGJMF) has shown promise in ameliorating hepatic steatosis in NASH patients, yet its pharmacological mechanisms remain largely unexplored. This study was conducted to investigate the efficacy of YGJMF in NASH and to elucidate its pharmacological underpinnings. To simulate NASH both <em>in vivo</em> and <em>in vitro</em>, high-fat-diet (HFD) rats and HepG2 cells stimulated with free fatty acids (FFAs) were utilized. The severity of liver injury and lipid deposition was assessed using serum indicators, histopathological staining, micro-magnetic resonance imaging (MRI), and the liver-to-muscle signal intensity ratio (SIR_L/M). Furthermore, a combination of enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), immunofluorescence, real-time quantitative polymerase chain reaction (RT-qPCR), and Western blotting analyses was employed to investigate the NF-<em>κ</em>B/NLRP3 signaling pathway and associated cytokine levels. The results from liver pathology, MRI assessments, and biochemical tests in rat models demonstrated YGJMF's significant effectiveness in reducing liver damage and lipid accumulation. Additionally, YGJMF markedly reduced hepatocyte inflammation by downregulating inflammatory cytokines in both liver tissue and serum. Furthermore, YGJMF was found to disrupt NF-<em>κ</em>B activation, consequently inhibiting the assembly of the NLRP3 inflammasome in both the <em>in vitro</em> and <em>in vivo</em> models. The preliminary findings of this study suggest that YGJMF may alleviate hepatic steatosis and inhibit the NF-<em>κ</em>B/NLRP3 signaling pathway, thereby exerting anti-inflammatory effects in NASH.</p></div>","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140309712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbonic anhydrase-like enzymes in the formation of Lycopodium alkaloid","authors":"Huiwen DONG,&nbsp;Huiming GE","doi":"10.1016/S1875-5364(24)60579-0","DOIUrl":"https://doi.org/10.1016/S1875-5364(24)60579-0","url":null,"abstract":"","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140309746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress in approved drugs from natural product resources","authors":"Zhongwen LUO,&nbsp;Fucheng YIN,&nbsp;Xiaobing WANG,&nbsp;Lingyi KONG","doi":"10.1016/S1875-5364(24)60582-0","DOIUrl":"https://doi.org/10.1016/S1875-5364(24)60582-0","url":null,"abstract":"<div><p>Natural products (NPs) have consistently played a pivotal role in pharmaceutical research, exerting profound impacts on the treatment of human diseases. A significant proportion of approved molecular entity drugs are either directly derived from NPs or indirectly through modifications of NPs. This review presents an overview of NP drugs recently approved in China, the United States, and other countries, spanning various disease categories, including cancers, cardiovascular and cerebrovascular diseases, central nervous system disorders, and infectious diseases. The article provides a succinct introduction to the origin, activity, development process, approval details, and mechanism of action of these NP drugs.</p></div>","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140309710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyparillums A and B: polycyclic polyprenylated acylphloroglucinols from Hypericum patulum","authors":"Yulin DUAN ,&nbsp;Zhengyi SHI ,&nbsp;Fei SONG ,&nbsp;Zhangrong HOU ,&nbsp;Xiaosheng TAN ,&nbsp;Yeting ZHANG ,&nbsp;Xincai HAO ,&nbsp;Gang CHEN ,&nbsp;Changxing QI ,&nbsp;Yonghui ZHANG","doi":"10.1016/S1875-5364(24)60599-6","DOIUrl":"https://doi.org/10.1016/S1875-5364(24)60599-6","url":null,"abstract":"<div><p>Hyparillums A (<strong>1</strong>) and B (<strong>2</strong>), two previously unidentified polycyclic polyprenylated acylphloroglucinols (PPAPs) with intricate architectures, were isolated from <em>Hypericum patulum</em> Thunb<em>.</em> Hyparillum A was the first PPAP with eight-carbon rings based on an unprecedented 6/6/5/6/6/5/6/4 octocyclic system featuring a rare heptacyclo[10.8.1.1^1,10.0^3,8.0^8,21.0^12,19.0^14,17]docosane core. In contrast, hyparillum B featured a novel heptacyclic architecture (6/6/5/6/6/5/5) based on a hexacyclo[9.6.1.1^1,9.0^3,7.0^7,18.0^11,16]nonadecane motif. Furthermore, hyparillums A and B demonstrated promising inhibitory effects on the proliferation of murine splenocytes stimulated by anti-CD3/anti-CD28 monoclonal antibodies and lipopolysaccharide, exhibiting half-maximal inhibitory concentration (IC₅₀) values ranging from 6.13 ± 0.86 to 12.69 ± 1.31 μmol·L⁻¹.</p></div>","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140309083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}