Chinese Journal of Natural Medicines最新文献

{"title":"Luteolin ameliorates ulcerative colitis in mice via reducing the depletion of NCR+ILC3 through Notch signaling pathway","authors":"Xueqian XIE ,&nbsp;Pengcheng LI ,&nbsp;Meng ZHAO ,&nbsp;Bo XU ,&nbsp;Guixing ZHANG ,&nbsp;Qing WANG ,&nbsp;Chen NI ,&nbsp;Xia LUO ,&nbsp;Lian ZHOU","doi":"10.1016/S1875-5364(24)60568-6","DOIUrl":"10.1016/S1875-5364(24)60568-6","url":null,"abstract":"<div><div>The disorder of group 3 innate lymphoid cells (ILC3) subgroup, such as the predominance of NCR^-ILC3 but the depletion of NCR⁺ILC3, is unfavorable to damaged intestinal barrier repair, which leads to the prolongations and obstinacy of ulcerative colitis (UC). Our previous studies had shown that luteolin promoted NCR⁻ILC3 differentitating into NCR⁺ILC3 to improving the depletion of NCR⁺ILC3 in UC mice, while the mechanism is unclear. This article aimed to explore the underlying mechanism of luteolin enhancing the proportion NCR⁺ILC3. UC mice model was established with 2% DSS and Notch signaling was blocked, then luteolin was used to intervene. The results showed that the effect of luteolin on ameliorating disease symptoms in UC mice, including inhibiting the weight loss, reducing the pathological damage of colon mucosa, <em>etc.</em>, was diminished with blocking Notch signaling pathway. In addition, luteolin increased the proportion of NCR⁺ILC3, NCR⁺MNK3 and IL-22⁺ILC3, decreased intestinal permeability, promoted mucin secretion, and promoted ZO-1 and Occludin expression, the above effect of luteolin was neutralized by Notch inhibitor LY-411575. Luteolin activated the abnormally blocked Notch signaling pathway in UC mice. And molecular docking predicted the affinity of luteolin for RBPJ to be −7.5 kcal·mol⁻¹ in mouse, respectively; the affinity of luteolin for Notch1 and RBPJ was respectively scored to be −6.4 kcal·mol⁻¹ and −7.7 kcal·mol⁻¹ homo sapiens. These results proved that luteolin is positive for enhancing the proportion of NCR⁺ILC3 <em>via</em> Notch signaling, and it provides a basis for targeting NCR⁺ILC3 for restoring intestinal barrier function to alleviating ulcerative colitis.</div></div>","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":"22 11","pages":"Pages 991-1002"},"PeriodicalIF":4.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142586880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The biologically and ecologically important natural products from the Chinese sea hare Bursatella leachii: structures, stereochemistry and beyond","authors":"Xinyuan ZHANG ,&nbsp;Mingzhi SU ,&nbsp;Mingxin ZHU ,&nbsp;Sha CHEN ,&nbsp;Zhen GAO ,&nbsp;Yuewei GUO ,&nbsp;Xuwen LI","doi":"10.1016/S1875-5364(24)60611-4","DOIUrl":"10.1016/S1875-5364(24)60611-4","url":null,"abstract":"<div><div>A novel amide alkaloid, bursatamide A (<strong>1</strong>), featuring an unprecedented propyl-hexahydronaphthalene carbon framework, was isolated from the infrequently studied sea hare <em>Bursatella leachi</em>, alongside a new 3-phenoxypropanenitrile alkaloid, bursatellin B (<strong>2</strong>), and twelve known compounds. The structures of <strong>1</strong> and <strong>2</strong> were elucidated through comprehensive spectroscopic data analyses, while their relative and absolute configurations (ACs) were established through total synthesis and a series of quantum chemical calculations, including calculated electronic circular dichroism (ECD) spectra, optical rotatory dispersion (ORD) methods, and DP4+ probability analyses. Bursatamide A (<strong>1</strong>) demonstrated inhibitory effects against the human pathogenic bacteria <em>Listeria monocytogenes</em> and <em>Vibrio cholerae. Erythro</em>-bursatellin B (<strong>21</strong>), a diastereoisomer of <strong>2</strong>, exhibited notable antibacterial activity against the fish pathogenic bacterium Streptococcus parauberis FP KSP28, with an MIC₉₀ value of 0.0472 μg·mL⁻¹.</div></div>","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":"22 11","pages":"Pages 1030-1039"},"PeriodicalIF":4.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142586889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multioxidized polyketides from an endophytic Penicillium sp. YUD17006 associated with Gastrodia elata","authors":"Hongtao LI ,&nbsp;Ruining YANG ,&nbsp;Fei XIE ,&nbsp;Tianpeng XIE ,&nbsp;Linhuan TANG ,&nbsp;Hao ZHOU ,&nbsp;Zhongtao DING","doi":"10.1016/S1875-5364(24)60724-7","DOIUrl":"10.1016/S1875-5364(24)60724-7","url":null,"abstract":"<div><div>Three novel, highly oxygenated polyketides, multioketides A−C (<strong>1</strong>−<strong>3</strong>), and three previously described multioxidized aromatic polyketides (<strong>4−6</strong>), were isolated from an endophytic <em>Penicillium</em> sp. YUD17006 associated with <em>Gastrodia elata</em>. Their chemical structures were elucidated using extensive spectroscopic data, electronic circular dichroism calculations, and single X-ray diffraction analysis. All metabolites were characterized by a typical <em>α</em>,<em>β</em>-unsaturated ketone fragment and exhibited a high degree of oxidation. Multioketides A and B were identified as a pair of epimers featuring a rare dihydroisobenzofuranone core. Multioketide C possessed a novel 5/6/6/6 heterotetracyclic chemical architecture with unusual 1,4-dioxin functionalities. Plausible biosynthetic pathways for <strong>1−6</strong> were proposed. Additionally, compound <strong>3</strong> demonstrated weak inhibitory activities against both acetylcholinesterase and protein tyrosine phosphatase 1B.</div></div>","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":"22 11","pages":"Pages 1057-1064"},"PeriodicalIF":4.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142586875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Talaketides A−G, linear polyketides with prostate cancer cytotoxic activity from the mangrove sediment-derived fungus Talaromyces sp. SCSIO 41027","authors":"Chunmei CHEN ,&nbsp;Xueni WANG ,&nbsp;Wenxuan FANG ,&nbsp;Jiaqi LIANG ,&nbsp;Jian CAI ,&nbsp;Dehua YANG ,&nbsp;Xiaowei LUO ,&nbsp;Chenghai GAO ,&nbsp;Xiangxi YI ,&nbsp;Yonghong LIU ,&nbsp;Xuefeng ZHOU","doi":"10.1016/S1875-5364(24)60659-X","DOIUrl":"10.1016/S1875-5364(24)60659-X","url":null,"abstract":"<div><div>Seven novel linear polyketides, talaketides A−G (<strong>1</strong>−<strong>7</strong>), were isolated from the rice media cultures of the mangrove sediment-derived fungus <em>Talaromyces</em> sp. SCSIO 41027. Among these, talaketides A−E (<strong>1</strong>−<strong>5</strong>) represented unprecedented unsaturated linear polyketides with an epoxy ring structure. The structures, including absolute configurations of these compounds, were elucidated through detailed analyses of nuclear magnetic resonance (NMR) and high-resolution mass spectrometry (HR-MS) data, as well as electronic custom distributors (ECD) calculations. In the cytotoxicity screening against prostate cancer cell lines, talaketide E (<strong>5</strong>) demonstrated a dose-dependent inhibitory effect on prostate cancer PC-3 cell lines, with an IC₅₀ value of 14.44 μmol·L⁻¹ . Moreover, compound <strong>5</strong> significantly inhibited the cloning formation of PC-3 cell lines and arrested the cell cycle in S-phase, ultimately inducing apoptosis. These findings indicate that compound <strong>5</strong> may serve as a promising lead compound for the development of a potential treatment for prostate cancer.</div></div>","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":"22 11","pages":"Pages 1047-1056"},"PeriodicalIF":4.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142586888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in intelligent mass spectrometry data processing technology for in vivo analysis of natural medicines","authors":"Simian CHEN ,&nbsp;Binxin DAI ,&nbsp;Dandan ZHANG ,&nbsp;Yuexin YANG ,&nbsp;Hairong ZHANG ,&nbsp;Junyu ZHANG ,&nbsp;Di LU ,&nbsp;Caisheng WU","doi":"10.1016/S1875-5364(24)60687-4","DOIUrl":"10.1016/S1875-5364(24)60687-4","url":null,"abstract":"<div><div>Natural medicines (NMs) are crucial for treating human diseases. Efficiently characterizing their bioactive components <em>in vivo</em> has been a key focus and challenge in NM research. High-performance liquid chromatography-high-resolution mass spectrometry (HPLC-HRMS) systems offer high sensitivity, resolution, and precision for conducting <em>in vivo</em> analysis of NMs. However, due to the complexity of NMs, conventional data acquisition, mining, and processing techniques often fail to meet the practical needs of <em>in vivo</em> NM analysis. Over the past two decades, intelligent spectral data-processing techniques based on various principles and algorithms have been developed and applied for <em>in vivo</em> NM analysis. Consequently, improvements have been achieved in the overall analytical performance by relying on these techniques without the need to change the instrument hardware. These improvements include enhanced instrument analysis sensitivity, expanded compound analysis coverage, intelligent identification, and characterization of nontargeted <em>in vivo</em> compounds, providing powerful technical means for studying the <em>in vivo</em> metabolism of NMs and screening for pharmacologically active components. This review summarizes the research progress on <em>in vivo</em> analysis strategies for NMs using intelligent MS data processing techniques reported over the past two decades. It discusses differences in compound structures, variations among biological samples, and the application of artificial intelligence (AI) neural network algorithms. Additionally, the review offers insights into the potential of <em>in vivo</em> tracking of NMs, including the screening of bioactive components and the identification of pharmacokinetic markers. The aim is to provide a reference for the integration and development of new technologies and strategies for future <em>in vivo</em> analysis of NMs.</div></div>","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":"22 10","pages":"Pages 900-913"},"PeriodicalIF":4.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chiral resolution of furofuran lignans and their derivatives from the stems of Dendrobium 'Sonia'","authors":"Kaimei QIU ,&nbsp;Hao QIU ,&nbsp;Yanqiao XIE ,&nbsp;Siyu ZHANG ,&nbsp;Qian ZHANG ,&nbsp;Zhengtao WANG ,&nbsp;Zhuzhen HAN ,&nbsp;Li YANG","doi":"10.1016/S1875-5364(24)60725-9","DOIUrl":"10.1016/S1875-5364(24)60725-9","url":null,"abstract":"<div><div>Five new furofuran lignans and their derivatives, (−)-glaberide I 4-<em>O</em>-<em>β</em>-D-glucopyranoside (<strong>1a</strong>), (+)-glaberide I 4-<em>O</em>-<em>β</em>-D-glucopyranoside (<strong>1b</strong>), (+)-glaberide I 7'-ethoxy-4-<em>O</em>-<em>β</em>-D-glucopyranoside (<strong>2a</strong>), (−)-glaberide I 7'-ethoxy-4-<em>O</em>-<em>β</em>-D-glucopyranoside (<strong>2b</strong>), and (−)-isoeucommin A (<strong>3b</strong>), along with fifteen known analogs were isolated from the stems of <em>Dendrobium</em> 'Sonia'. These compounds were classified into ten pairs of enantiomers or diastereoisomers <em>via</em> chiral resolution, and their structures were determined based on extensive spectroscopic data. Their absolute configurations were determined by hydrolysis, comparison of experimental and calculated electronic circular dichroism (ECD) data, and single-crystal X-ray diffraction analysis. The isolates were evaluated for their ability to inhibit nitric oxide (NO) production in RAW264.7 cells. Among them, syringaresinol (<strong>5</strong>) exhibited prominent inhibition activity, with an IC₅₀ value of 28.4 ± 3.0 μmol·L⁻¹, and there was a slight difference between <strong>5a</strong>, <strong>5b</strong> and the racemic mixture <strong>5</strong>.</div></div>","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":"22 10","pages":"Pages 937-944"},"PeriodicalIF":4.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly oxygenated clerodane furanoditerpenoids from the leaves and twigs of Croton yunnanensis","authors":"Didi WANG ,&nbsp;Fang MEI ,&nbsp;Jinchun NIE ,&nbsp;Zhenwei LI ,&nbsp;Daidi ZHANG ,&nbsp;Dean GUO ,&nbsp;Wei LI","doi":"10.1016/S1875-5364(24)60722-3","DOIUrl":"10.1016/S1875-5364(24)60722-3","url":null,"abstract":"<div><div>The phytochemical investigation of the leaves and twigs of <em>Croton yunnanensis</em> resulted in the isolation of eight new clerodane furanoditerpenoids, named croyunfuranoids A−H (<strong>1</strong>−<strong>8</strong>), along with three known analogs (<strong>9</strong>−<strong>11</strong>). The structures of these compounds were elucidated using spectroscopic analyses, and their absolute configurations were determined through a combination of electronic circular dichroism (ECD) calculations and single-crystal X-ray diffraction. Notably, Croyunfuranoid D (<strong>4</strong>) is identified as a rare 18,19-<em>dinor</em>-clerodane diterpenoid. Additionally, the structure of a previously reported diterpenoid, crotonyunnan B, was revised. All isolated compounds were evaluated for their inhibitory activities on nitric oxide (NO) production in LPS-induced RAW 264.7 macrophages. Among them, compounds <strong>5</strong> and <strong>6</strong> demonstrated significant inhibitory effects, with IC₅₀ values of 20.33 ± 2.31 and 22.80 ± 1.31 μmol·L⁻¹, respectively.</div></div>","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":"22 10","pages":"Pages 945-954"},"PeriodicalIF":4.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insight into targeting the DNA damage response in the treatment of glioblastoma","authors":"Tengfei ZHEN ,&nbsp;Tianyu SUN ,&nbsp;Baichen XIONG ,&nbsp;Hui LIU ,&nbsp;Lei WANG ,&nbsp;Yao CHEN ,&nbsp;Haopeng SUN","doi":"10.1016/S1875-5364(24)60694-1","DOIUrl":"10.1016/S1875-5364(24)60694-1","url":null,"abstract":"<div><div>Glioblastoma (GBM) is the most common invasive malignant tumor in human brain tumors, representing the most severe grade of gliomas. Despite existing therapeutic approaches, patient prognosis remains dismal, necessitating the exploration of novel strategies to enhance treatment efficacy and extend survival. Due to the restrictive nature of the blood-brain barrier (BBB), small-molecule inhibitors are prioritized in the treatment of central nervous system tumors. Among these, DNA damage response (DDR) inhibitors have garnered significant attention due to their potent therapeutic potential across various malignancies. This review provides a detailed analysis of DDR pathways as therapeutic targets in GBM, summarizes recent advancements, therapeutic strategies, and ongoing clinical trials, and offers perspectives on future directions in this rapidly evolving field. The goal is to present a comprehensive outlook on the potential of DDR inhibitors in improving GBM management and outcomes.</div></div>","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":"22 10","pages":"Pages 869-886"},"PeriodicalIF":4.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting TLR4 and regulating the Keap1/Nrf2 pathway with andrographolide to suppress inflammation and ferroptosis in LPS-induced acute lung injury","authors":"Yichen LI ,&nbsp;Liting HUANG ,&nbsp;Jilang LI ,&nbsp;Siyuan LI ,&nbsp;Jianzhen LV ,&nbsp;Guoyue ZHONG ,&nbsp;Ming GAO ,&nbsp;Shilin YANG ,&nbsp;Shan HAN ,&nbsp;Wenhui HAO","doi":"10.1016/S1875-5364(24)60727-2","DOIUrl":"10.1016/S1875-5364(24)60727-2","url":null,"abstract":"<div><div>Acute lung injury (ALI) is a severe inflammatory condition with a high mortality rate, often precipitated by sepsis. The pathophysiology of ALI involves complex mechanisms, including inflammation, oxidative stress, and ferroptosis, a novel form of regulated cell death. This study explores the therapeutic potential of andrographolide (AG), a bioactive compound derived from <em>Andrographis</em>, in mitigating Lipopolysaccharide (LPS)-induced inflammation and ferroptosis. Our research employed <em>in vitro</em> experiments with RAW264.7 macrophage cells and <em>in vivo</em> studies using a murine model of LPS-induced ALI. The results indicate that AG significantly suppresses the production of pro-inflammatory cytokines and inhibits ferroptosis in LPS-stimulated RAW264.7 cells. <em>In vivo</em>, AG treatment markedly reduces lung edema, decreases inflammatory cell infiltration, and mitigates ferroptosis in lung tissues of LPS-induced ALI mice. These protective effects are mediated <em>via</em> the modulation of the Toll-like receptor 4 (TLR4)/Kelch-like ECH-associated protein 1(Keap1)/Nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway. Molecular docking simulations identified the binding sites of AG on the TLR4 protein (Kd value: −33.5 kcal·mol⁻¹), and these interactions were further corroborated by Cellular Thermal Shift Assay (CETSA) and SPR assays. Collectively, our findings demonstrate that AG exerts potent anti-inflammatory and anti-ferroptosis effects in LPS-induced ALI by targeting TLR4 and modulating the Keap1/Nrf2 pathway. This study underscores AG’s potential as a therapeutic agent for ALI and provides new insights into its underlying mechanisms of action.</div></div>","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":"22 10","pages":"Pages 914-928"},"PeriodicalIF":4.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Four new diarylheptanoids and two new terpenoids from the fruits of Alpinia oxyphylla and their anti-inflammatory activities","authors":"Jie DONG ,&nbsp;Haibo LI ,&nbsp;Mi ZHOU ,&nbsp;Xinsheng YAO ,&nbsp;Jianliang GENG ,&nbsp;Yang YU","doi":"10.1016/S1875-5364(24)60723-5","DOIUrl":"10.1016/S1875-5364(24)60723-5","url":null,"abstract":"<div><div>Four previously unreported diarylheptanoids (<strong>1a</strong>/<strong>1b</strong>−<strong>2a</strong>/<strong>2b</strong>), one undescribed sesquiterpenoid (<strong>8</strong>), one new diterpenoid (<strong>12</strong>), and twelve known analogs were isolated from the fruits of <em>Alpinia oxyphylla</em>. The structural elucidation of these compounds was achieved through a comprehensive analysis of spectroscopic data, single-crystal X-ray diffraction, electronic circular dichroism (ECD), and modified Mosher’s method. Enantiomeric mixtures (<strong>1a</strong>/<strong>1b</strong>, <strong>2a</strong>/<strong>2b</strong>, <strong>3a</strong>/<strong>3b</strong>, <strong>4a</strong>/<strong>4b</strong>, and <strong>5a</strong>/<strong>5b</strong>) were separated on a chiral column using acetonitrile-water mixtures as eluents. Among them, compounds <strong>3a</strong>/<strong>3b</strong> and <strong>4a</strong>/<strong>4b</strong> were isolated as optically pure enantiomers in the initial chiral separation. Furthermore, most of the isolates were evaluated for their inhibitory effects against the production of nitric oxide (NO) and interleukin-6 (IL-6) in lipopolysaccharide (LPS)-induced RAW264.7 macrophages. Interestingly, <strong>2</strong> and <strong>4</strong> showed significant inhibitory activities against NO production with IC₅₀ values of 33.65 and 9.88 μmol·L⁻¹ (hydrocortisone: IC₅₀ 34.26 μmol·L⁻¹), respectively. Additionally, they also partially reduced the secretion of IL-6.</div></div>","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":"22 10","pages":"Pages 929-936"},"PeriodicalIF":4.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}