Advanced Agrochem最新文献_第7页

Aptamers for mycotoxin recognition in food: Recent advances and future considerations 食品中霉菌毒素识别的适体:最新进展和未来考虑

Advanced Agrochem Pub Date : 2023-09-01 DOI: 10.1016/j.aac.2023.06.002

Peng Fang, Hao Qu, Yu Mao, Lei Zheng

引用次数: 1

DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stages 蜡样芽孢杆菌不同生长阶段DNA适体的选择与鉴定

Advanced Agrochem Pub Date : 2023-09-01 DOI: 10.1016/j.aac.2023.07.005

Nuo Duan , Mingyue Ye , Minghui Lu , Xiaowan Chen , Shijia Wu

{"title":"DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stages","authors":"Nuo Duan , Mingyue Ye , Minghui Lu , Xiaowan Chen , Shijia Wu","doi":"10.1016/j.aac.2023.07.005","DOIUrl":"https://doi.org/10.1016/j.aac.2023.07.005","url":null,"abstract":"<div><p>The physiology (membrane integrity, intracellular esterase and redox activity) of individual pathogenic bacteria is various in different growing phase, which has a profound significance for food safety testing and control. In this work, the aptamers against <em>B. cereus</em> in different growing phase was firstly selected based on whole bacterium-SELEX screening approach. Out of 32 candidate aptamers screened, aptamer B15 and B16 showed relatively high binding affinity with an apparent dissociation constant (Kd value) of 16.13 ± 4.98 nM and 20.67 ± 5.23 nM, respectively. A label-free impedance based aptasensor was further fabricated based on the screened aptamer, immobilized onto the GCE/Au surface. With the addition of <em>B. cereus</em>, the electron transfer process was prevented and exhibited a significant decrease in the electron-transfer resistance. Under optimal conditions, the fabricated aptasensor can achieve high sensitive detection of <em>B. cereus</em> as low as 10 cfu/mL. A satisfactory recovery of 90.5%–105.3% was achieved for the <em>B. cereus</em> detection in spiked milk samples, exhibiting its prospect in the sensitive detection of <em>B. cereus</em> in daily life.</p></div>","PeriodicalId":100027,"journal":{"name":"Advanced Agrochem","volume":"2 3","pages":"Pages 284-290"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Functional nucleic acid circuitry as a universal detector for pathogens 功能性核酸电路作为病原体的通用检测器

Advanced Agrochem Pub Date : 2023-09-01 DOI: 10.1016/j.aac.2023.06.005

Chunxu Yu , Rujian Zhao , Yidan Tang , Siyan Zhou , Xin Zhao , Baiyang Lu , Fanzheng Meng , Bingling Li

{"title":"Functional nucleic acid circuitry as a universal detector for pathogens","authors":"Chunxu Yu , Rujian Zhao , Yidan Tang , Siyan Zhou , Xin Zhao , Baiyang Lu , Fanzheng Meng , Bingling Li","doi":"10.1016/j.aac.2023.06.005","DOIUrl":"https://doi.org/10.1016/j.aac.2023.06.005","url":null,"abstract":"<div><p>While the pathogen nucleic acid diagnostic technology has made tremendous progresses, several challenges, including long development cycles and limited specificity still exist, especially in the context of isothermal amplification techniques. To enhance the detection accuracy, here a functional strand displacement catalytic hairpin assembly circuit, which can perform at high-temperature (HT-CHA), was developed as the downstream of the loop mediated isothermal nucleic acid amplification (LAMP). The addition of HT-CHA not only ensures the specificity but also amplifies the detection signal. Taking African swine fever (ASF) gene as the target, the input of HT-CHA was designed according to the ASF gene LAMP amplicons. This LAMP-HTCHA can detect as low as 2 copies/μL targeting genes with high signal-to-noise ratio. Through importing a three-way junction (3WJ) transducer, the HT-CHA well-developed for ASF gene can be directly adapted to detect the LAMP amplicons of other pathogen genes, such as Mycoplasma pneumoniae (MP) gene. The time-consuming and high-risk process to redesign HT-CHA components can be further avoided, making the method even general and useful for a plenty of other targets. Finally, the accurate detection of MP gene in alveolar lavage fluid samples confirmed the high potential of the LAMP and HT-CHA combination in clinical applications, providing a promising strategy to develop point-of-care diagnostics at constant temperatures.</p></div>","PeriodicalId":100027,"journal":{"name":"Advanced Agrochem","volume":"2 3","pages":"Pages 291-296"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Functional nucleic acid-based biosensors for virus detection 用于病毒检测的功能性核酸生物传感器

Advanced Agrochem Pub Date : 2023-09-01 DOI: 10.1016/j.aac.2023.07.006

Zijie Zhang , Bal Ram Adhikari , Payel Sen , Leyla Soleymani , Yingfu Li

{"title":"Functional nucleic acid-based biosensors for virus detection","authors":"Zijie Zhang , Bal Ram Adhikari , Payel Sen , Leyla Soleymani , Yingfu Li","doi":"10.1016/j.aac.2023.07.006","DOIUrl":"https://doi.org/10.1016/j.aac.2023.07.006","url":null,"abstract":"<div><p>A growing number of viruses pose significant threats to human health, impact agricultural production and compromise food safety. Minimizing the impact of viral infections demands for effective technologies that can deliver sensitive viral detection under various settings. Functional nucleic acids (FNAs) – natural or synthetic nucleic acid molecules with catalytic and/or binding properties – have been increasingly explored as key components for setting up diagnostic tests for viral detection. FNA-based viral biosensors offer distinct advantages, such as the ability to achieve sensitive viral detection in complex biological samples, ease of use, scalability, and cost-effectiveness. This mini-review will be dedicated to appraising progress made in recent years in FNA-based biosensors for diagnosing diverse human and animal viruses. We will first describe virus structures and potential biomarkers that can be utilized as the targets for FNA probe engineering. This is followed by reviewing the latest advances in two classes of FNAs - DNAzyme and DNA aptamers, with focuses on DNAzyme- and DNA aptamer-based biosensors that employ diverse signal transduction mechanisms to achieve sensitive viral detections. Finally, we will discuss current challenges in the field and suggest future research directions that can address these challenges.</p></div>","PeriodicalId":100027,"journal":{"name":"Advanced Agrochem","volume":"2 3","pages":"Pages 246-257"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Dynamic DNA nanomachines for amplification imaging of diseased cells based on stimuli-responsive mechanism 基于刺激反应机制的病变细胞扩增成像的动态DNA纳米机

Advanced Agrochem Pub Date : 2023-09-01 DOI: 10.1016/j.aac.2023.06.001

Jingting Wu, Wenqing Lin, Zai-Sheng Wu

{"title":"Dynamic DNA nanomachines for amplification imaging of diseased cells based on stimuli-responsive mechanism","authors":"Jingting Wu, Wenqing Lin, Zai-Sheng Wu","doi":"10.1016/j.aac.2023.06.001","DOIUrl":"https://doi.org/10.1016/j.aac.2023.06.001","url":null,"abstract":"<div><p>As a basic functional unit, living cell with sophisticated structures play an indispensable role in life activities. Since the abnormality of important molecules inside cells is closely related to diseases, the dynamic analysis and spatio-temporal monitoring of specific molecules in living cells can provide precious information for the diagnosis and treatment of diseases. More recently, DNA has not only been recognized as the carrier of genetic information, but has also used as a robust building block for the assembly of multitudinous nanoscale structures due to the intrinsic advantages of high programmability of classic Watson–Crick base-pairing rule. Intensive study promotes the rapid progress of nanotechnology in various fields, such as bioimaging, diagnosis, and therapeutics. Among numerous well-defined DNA nanomaterials, DNA nanomachines have been widely exploited in cell imaging owing to their desirable ability to achieve high-resolution temporal and spatial images in response to endogenous or exogenous stimuli. In brief, elaborate DNA nanomachines can undergo structural changes upon the stimuli of target analytes or environmental factors, resulting in rapid increase or reduction of output signals and thereby indirectly reflecting the expression level of targets. DNA nanomachines with high sensitivity and specificity contribute to the recognition of diseased tissues. In this review, we introduce the basic assembly modules of DNA nanomachines and summarize the recent advances in dynamic DNA nanomachines for diseased-cell imaging. Finally, the current challenges and future directions of DNA nanomachines for bioimaging are discussed.</p></div>","PeriodicalId":100027,"journal":{"name":"Advanced Agrochem","volume":"2 3","pages":"Pages 202-212"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A novel fluorescent structure-switching aptasensor for the sensitive detection of acrylamide based on AuNPs-assisted separation of ssDNA 一种基于aunps辅助分离ssDNA的新型丙烯酰胺荧光开关适配体传感器

Advanced Agrochem Pub Date : 2023-09-01 DOI: 10.1016/j.aac.2023.07.001

Ningyi Xu , Xinyue Ma , Yue Cao , Huan Wang , Han Wu , Hongru Zheng , Chuanyu Yang , Ruiqi Zou , Jiayi Liu , Chunyan Sun , Yuan Yuan

{"title":"A novel fluorescent structure-switching aptasensor for the sensitive detection of acrylamide based on AuNPs-assisted separation of ssDNA","authors":"Ningyi Xu , Xinyue Ma , Yue Cao , Huan Wang , Han Wu , Hongru Zheng , Chuanyu Yang , Ruiqi Zou , Jiayi Liu , Chunyan Sun , Yuan Yuan","doi":"10.1016/j.aac.2023.07.001","DOIUrl":"https://doi.org/10.1016/j.aac.2023.07.001","url":null,"abstract":"<div><p>Fluorescent aptasensor was developed for acrylamide (AAm) detection by utilizing the adsorption effect of Au nanoparticles (AuNPs) and fluorescence properties of SYBR Green I (SGI) towards double-stranded DNA (dsDNA). Compared to the binding of aptamer with AAm, the higher affinity of aptamer with cDNA may facilitate a structure switching from the aptamer/AAm complex to aptamer/cDNA dsDNA. The free aptamers were adsorbed onto AuNPs and separated by centrifugation. Subsequently, SGI was introduced as a fluorescent reporter for quantitative detection. Compared to conventional AuNPs-based colorimetric detection, the sensitivity of this strategy was improved by 3.18-fold in the range of 0.005–50 mg/L with a low detection limit of 4.68 μg/L. The method has been successfully applied to analyze fried twists and biscuits. Notably, it is a low-cost and general method that provides guidance for the development of rapid screening technology in the field.</p></div>","PeriodicalId":100027,"journal":{"name":"Advanced Agrochem","volume":"2 3","pages":"Pages 276-283"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Colorimetric detection of aflatoxins B1 and M1 using aptamers and gold and silver nanoparticles 利用适体和金、银纳米颗粒比色法检测黄曲霉毒素B1和M1

Advanced Agrochem Pub Date : 2023-09-01 DOI: 10.1016/j.aac.2023.07.003

Fiona Ebanks , Hadi Nasrallah , Timothy M. Garant , Erin M. McConnell , Maria C. DeRosa

{"title":"Colorimetric detection of aflatoxins B1 and M1 using aptamers and gold and silver nanoparticles","authors":"Fiona Ebanks , Hadi Nasrallah , Timothy M. Garant , Erin M. McConnell , Maria C. DeRosa","doi":"10.1016/j.aac.2023.07.003","DOIUrl":"https://doi.org/10.1016/j.aac.2023.07.003","url":null,"abstract":"<div><p>Mycotoxins are small molecules produced by fungi that contaminate crops and cause notable health effects for humans, owing to their inherent toxicity. Aflatoxins are among the most potent mycotoxins, with aflatoxin B1 and M1 being the most concerning. Due to their negative impact on human health and agro-economics, developing cost-effective, rapid, highly sensitive and specific detection tools is urgently needed. Nucleic acid–based synthetic receptors, aptamers, have been successfully selected for aflatoxin with high binding affinity and selectivity, and have been incorporated into a wide array of sensor platforms. By exploiting the optical properties of metallic nanoparticles, aptasensors have been developed to achieve low-cost, rapid, and sensitive tests to detect contaminated foods. Herein, we describe the use of functional nucleic acids, specifically DNA aptamers with metallic nanoparticles such as gold and silver for detecting aflatoxin B1 and M1. This review highlights various aptamer-nanoparticle assay types designed for colorimetric aflatoxin detection (i.e., solution and paper-based) along with their associated detection limits, as well as their strengths and areas for further development.</p></div>","PeriodicalId":100027,"journal":{"name":"Advanced Agrochem","volume":"2 3","pages":"Pages 221-230"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Competitive aptamer switch for modulating ligand binding affinity 调节配体结合亲和力的竞争性适配体开关

Advanced Agrochem Pub Date : 2023-09-01 DOI: 10.1016/j.aac.2023.06.004

Derek Puyat , Sung Won Oh , Shiming Liu , Jinglin Fu Ph.D

{"title":"Competitive aptamer switch for modulating ligand binding affinity","authors":"Derek Puyat , Sung Won Oh , Shiming Liu , Jinglin Fu Ph.D","doi":"10.1016/j.aac.2023.06.004","DOIUrl":"https://doi.org/10.1016/j.aac.2023.06.004","url":null,"abstract":"<div><p>Aptamers are short, single-stranded DNA or RNA molecules that selectively bind to a target molecule. Aptamer-complement duplex (ACD) is often used to design molecular switches capable of producing a detectable signal or triggering a structural change upon aptamer binding to a target. However, such aptamer switch generally faces an increased dissociation constant (K<sub>d</sub>) due to the energy barrier of the complementary duplex. We reported a competitive hybridization mechanism to modulate the binding affinity of an ACD to a target adenosine. Using the computation-guided design, we calculated the aptamer folding energy for the duplex length from 11-nt to 15-nt, and experimentally measured increased apparent K<sub>d</sub> values resulted from these extended duplexes. Using a set of strands to compete with the ACD hybridization, it reduced the aptamer folding energy to facilitate aptamer switches with decreased apparent K<sub>d</sub> values ranging from over 400 μM without a competing strand to ∼30 μM with a competing strand. This competitive aptamer switch was also found sensitive to single-nucleotide mutations of a competing strand. Our work provides an approach to modulate the binding affinity and the sensitivity of aptamer-complement duplexes, which could be useful in the nucleic acids-based sensing and nanomedicine.</p></div>","PeriodicalId":100027,"journal":{"name":"Advanced Agrochem","volume":"2 3","pages":"Pages 264-268"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Research progress of DNA aptamer-based silver ions detection 基于DNA适配体的银离子检测研究进展

Advanced Agrochem Pub Date : 2023-09-01 DOI: 10.1016/j.aac.2023.06.006

Yushi Xie , Min Yang , Longjiao Zhu , Xiaoyu Yue , Baiqiang Zhai , Wentao Xu

引用次数: 1

Application of photopharmacology in agrochemicals 光药理学在农药中的应用

Advanced Agrochem Pub Date : 2023-06-01 DOI: 10.1016/j.aac.2023.04.002

Wen Fu , Zhong Li , Xusheng Shao

{"title":"Application of photopharmacology in agrochemicals","authors":"Wen Fu , Zhong Li , Xusheng Shao","doi":"10.1016/j.aac.2023.04.002","DOIUrl":"https://doi.org/10.1016/j.aac.2023.04.002","url":null,"abstract":"<div><p>Photopharmacology is a novel technology in drug design that aims at solving poor drug selectivity. This technology is currently in the proof-of-concept phase and relies on the photoactivation or inactivation of photochromic ligands (PCLs) to regulate biological functions and living organisms. As potential molecular tools in future agriculture 4.0, the photochromic pesticides are effective in optical control of receptors, ion channels, living behaviors, and enzymes, which displays an innovative way of pesticide discovery. In this review, we highlight the progresses of the photoisomerized insecticides and fungicides. By integrating photoswitches, such as azobenzenes and diarylethenes, into pesticide molecules, several PCLs were developed for optical regulation of important insect or fungi targets in vivo, including GABARs, RyRs, nAChRs, SURs, sodium channels, GluCls, and SDH. The results are highly significant in revealing interactions of agrochemicals with their targets, biological functions, and living behaviors, and provide powerful toolkits in understanding ligand-receptor interactions. Moreover, there is a growing demand for a diverse range of PCLs, particularly those that are sensitive to red and NIR light or sunlight, for actual field use in agriculture. We envision that this particular mode of pesticide discovery is of great promise for overcoming the challenges posed by the improper use of agrochemicals.</p></div>","PeriodicalId":100027,"journal":{"name":"Advanced Agrochem","volume":"2 2","pages":"Pages 142-153"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5