IF 5.1 2区医学

Glia Pub Date : 2025-10-01 Epub Date: 2025-07-17 DOI: 10.1002/glia.70050

Caela C Long, Lindsay K Festa, Melanie Cruz-Berrios, Teshawn D Johnson, Claire H Mitchell, Kelly L Jordan-Sciutto, Judith B Grinspan

{"title":"Acidic Nanoparticles Prevent HIV Pre-Exposure Prophylaxis (PrEP)-Induced Oligodendrocyte Impairments by Restoring Lysosomal pH in Adolescent Models.","authors":"Caela C Long, Lindsay K Festa, Melanie Cruz-Berrios, Teshawn D Johnson, Claire H Mitchell, Kelly L Jordan-Sciutto, Judith B Grinspan","doi":"10.1002/glia.70050","DOIUrl":"10.1002/glia.70050","url":null,"abstract":"A disproportionate percentage of adolescents are diagnosed with human immunodeficiency virus (HIV) in the United States each year. Preexposure prophylaxis (PrEP), an antiretroviral regimen, is effective at preventing the transmission of HIV to adolescents at substantial risk for acquiring HIV. However, other select antiretrovirals have been shown to cause white matter deficits in experimental models. Adolescents taking PrEP are uniquely vulnerable to myelin impairments as the adolescent brain undergoes high rates of myelination. Here, we report that PrEP significantly reduced oligodendrocyte maturation in adolescent rats. Furthermore, cultures of primary rat oligodendrocyte progenitors treated with PrEP showed inhibited oligodendrocyte differentiation through deacidification of lysosomes resulting in lysosomal accumulation of myelin proteins. Acidic nanoparticle co-administration with PrEP prevented PrEP-induced oligodendrocyte maturation impairments both in vivo and in vitro. These studies suggest uninfected adolescents are vulnerable to PrEP-induced oligodendrocyte impairments and identify maintenance of lysosome pH as a critical factor in antiretroviral design.","PeriodicalId":174,"journal":{"name":"Glia","volume":" ","pages":"1967-1988"},"PeriodicalIF":5.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12334864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of bridging therapies in clinical trials leading to FDA approval of CAR-T cell therapies. 临床试验中桥接疗法的特性导致FDA批准CAR-T细胞疗法。

IF 4.7 2区医学

International Journal of Cancer Pub Date : 2025-10-01 Epub Date: 2025-05-20 DOI: 10.1002/ijc.35473

Victoria Kaestner, Alyson Haslam, Vinay Prasad

{"title":"Characterization of bridging therapies in clinical trials leading to FDA approval of CAR-T cell therapies.","authors":"Victoria Kaestner, Alyson Haslam, Vinay Prasad","doi":"10.1002/ijc.35473","DOIUrl":"10.1002/ijc.35473","url":null,"abstract":"During the time of chimeric antigen receptor T-cell (CAR-T) manufacturing, bridging therapy is often used to control disease. Because it often involves systemic treatment, the bridging therapies can induce responses and/or adverse events. We sought to assess bridging therapies used in CAR-T trials in a cross-sectional study. We reviewed FDA drug labels and peer-reviewed registration trial reports (including supplemental data) to evaluate the characteristics of bridging therapy used in trials testing CAR-T therapies. We looked at which bridging therapies were used, whether multiple therapies were combined, the response rates, and the reported adverse events associated with bridging therapy. Of the 11 studies testing CAR-T therapies, 10 reported the bridging therapies that were used in the study. Of those that reported the types of bridging therapies (n = 10), the most commonly used bridging therapy was dexamethasone (10/10, 100%), rituximab (6/10, 60%), gemcitabine (5/10, 50%), and etoposide (5/10, 50%). Of the trials, one of 11 (9%) clearly reported whether patients had responses to bridging therapy, six of 11 (55%) vaguely reported responses, and four of 11 (36%) trials did not report or mention any response information regarding bridging therapy. Although patients are often refractory to first-line therapies, which share considerable overlap with bridging therapies, these therapies may induce responses. Despite this possibility, the reporting of bridging therapy combinations and their subsequent response rates and adverse event rates are highly variable. These findings highlight the need for greater transparency in the reporting of bridging therapy to more reliably assess the efficacy of CAR-T therapies.","PeriodicalId":180,"journal":{"name":"International Journal of Cancer","volume":" ","pages":"1376-1385"},"PeriodicalIF":4.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SUR1-TRPM4 Regulates Aquaporin-4 Subcellular Localization by Astrocytic Endfeet Calcium Signals Following Status Epilepticus. SUR1-TRPM4通过星形细胞终足钙信号调控水通道蛋白-4亚细胞定位。

IF 5.1 2区医学

Glia Pub Date : 2025-10-01 Epub Date: 2025-07-09 DOI: 10.1002/glia.70056

Tingting Yang, Zhenzhou Lin, Mingjia Yu, Yongchuan Li, Jiancong Chen, Yuanchi Liu, Kaibin Huang, Suyue Pan

{"title":"SUR1-TRPM4 Regulates Aquaporin-4 Subcellular Localization by Astrocytic Endfeet Calcium Signals Following Status Epilepticus.","authors":"Tingting Yang, Zhenzhou Lin, Mingjia Yu, Yongchuan Li, Jiancong Chen, Yuanchi Liu, Kaibin Huang, Suyue Pan","doi":"10.1002/glia.70056","DOIUrl":"10.1002/glia.70056","url":null,"abstract":"Status epilepticus (SE) is a severe condition that results in uncontrollable cerebral edema and cognitive dysfunction. Recent studies suggest that the localization of aquaporin-4 (AQP4) in astrocytic endfeet plays a crucial role in regulating blood-brain water transport and cell volume control, particularly along perivascular pathways. However, the signaling mechanisms underlying AQP4 localization remain poorly understood. In this study, we utilized the genetically encoded fluorescent calcium (Ca2+) indicator GCaMp6f to investigate Ca2+ signals in astrocytic somata, processes, and endfeet during SE induction and observed enhanced Ca2+ signals in both the somata and perivascular endfeet of astrocytes. We employed genetic knockout of TRPM4 (Trpm4 -/- ) and glibenclamide treatment to explore the role of sulfonylurea receptor 1 transient receptor potential melastatin-4 (SUR1-TRPM4) channel in these Ca2+ responses. Both approaches significantly suppressed the Ca2+ signals in the astrocytic endfeet and reduced perivascular expression of the Ca2+-related signaling pathway sensor calmodulin (CaM). Furthermore, we found that AQP4 localization was no longer confined to the domains of astrocytic endfeet following SE. Inhibition of SUR1-TRPM4 through pharmacological blockade or gene deletion restored the subcellular localization of AQP4, reduced cerebral edema, and improved cognitive outcomes post-SE. Our findings suggest that SUR1-TRPM4 plays a pivotal role in regulating astrocytic Ca2+ signals and mediating the aberrant expression and subcellular localization of astrocytic AQP4 along perivascular pathways. Together, these findings demonstrate a novel molecular mechanism underscoring SUR1-TRPM4 therapy in the treatment of SE characterized by dysregulated Ca2+ signaling in astrocytic endfeet.","PeriodicalId":174,"journal":{"name":"Glia","volume":" ","pages":"2057-2076"},"PeriodicalIF":5.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Biology, Microclimate, and Geology of a Distinctive Ecosystem Within the Sandstone of Hyper-Arid Timna Valley, Israel 以色列极度干旱的提姆纳河谷砂岩中独特生态系统的生物学、小气候和地质学

IF 2.7 4区生物学

Environmental Microbiology Reports Pub Date : 2025-09-15 DOI: 10.1111/1758-2229.70188

Irit Nir, Rachel Armoza-Zvuloni, Hana Barak, Asunción De los Ríos, Christopher P. McKay, Ariel Kushmaro

{"title":"The Biology, Microclimate, and Geology of a Distinctive Ecosystem Within the Sandstone of Hyper-Arid Timna Valley, Israel","authors":"Irit Nir, Rachel Armoza-Zvuloni, Hana Barak, Asunción De los Ríos, Christopher P. McKay, Ariel Kushmaro","doi":"10.1111/1758-2229.70188","DOIUrl":"https://doi.org/10.1111/1758-2229.70188","url":null,"abstract":"Microbial endolithic communities in the sandstone rocks of the southern Negev Desert, particularly in Timna Park, were initially discovered by Imre Friedmann and Roseli Ocampo-Friedmann in their pioneering study about 50 years ago. Nonetheless, this harsh microecosystem, dominated by cyanobacterial taxa, raises questions about the adaptive mechanisms that enable the survival of these microorganisms. The present study provides comprehensive data, including extensive precipitation records for the Timna Valley, and multi-year microclimatic data from a colonised site. It includes examinations of rock structure, as well as microscopic and metagenomic analysis. Our findings point to a distinct bacterial endolithic population dominated by the cyanobacterial genus Chroococcidiopsis. Although the taxa are well known, we show here how their exclusive persistence is driven by the sandstone's fine porosity and thermal properties, combined with rare, low-volume precipitation. This highly selective microenvironment highlights how specific rock and climate interactions can filter microbial diversity in hyper-arid deserts. Additionally, it demonstrates an adaptation strategy based on both short-term and decadal-scale dormancy. Thus, it offers new insights for the survival of these unique ecosystems and provides valuable perspectives for astrobiology and the search for evidence of microbial life on Mars.","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70188","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamics of soil organic carbon, nitrogen, phosphorus, and aggregates stability under different grassland management types in the central Rift Valley, Ethiopia 埃塞俄比亚中部大裂谷不同草地管理方式下土壤有机碳、氮、磷和团聚体稳定性动态

IF 1.5

Agrosystems, Geosciences & Environment Pub Date : 2025-09-15 DOI: 10.1002/agg2.70195

Wakshuma Y. Mergo, Gianni Bellocchi

{"title":"Dynamics of soil organic carbon, nitrogen, phosphorus, and aggregates stability under different grassland management types in the central Rift Valley, Ethiopia","authors":"Wakshuma Y. Mergo, Gianni Bellocchi","doi":"10.1002/agg2.70195","DOIUrl":"https://doi.org/10.1002/agg2.70195","url":null,"abstract":"Grassland management significantly influences soil organic carbon (SOC) and nutrient fluxes. This study investigated the effects of five distinct grassland management types on SOC, nitrogen (N), and phosphorus (P) in Ethiopia's Central Rift Valley. We collected soil samples from random grazing natural grasslands (random grazed); periodically mowed and grazed natural grasslands (mowed-grazed); cultivated, fertilized, and mowed grasslands (cultivated-mowed); periodically mowed area closure natural grasslands (mowed-closed); and protected native forest grasslands (protected forest). We analyzed SOC, labile and stable C fractions, total N, total P, and soil aggregate indices using standard methods, also calculating the C management index and stability ratio. Our findings reveal significant variations (p < 0.01) in SOC fractions across management types. Protected forest grasslands exhibited the highest labile C (1.41%), while random grazed grasslands showed the lowest (0.39%). For stable C, mowed-closed grasslands had the highest (0.92%) and random grazed the lowest (0.23%). Total N ranged from 1.18% in protected forest and cultivated-mowed to 0.04% in random grazed, with total P highest in cultivated-mowed soils. SOC, total N, and total P levels also varied significantly (p < 0.05) with soil depth. The highest total SOC was observed in protected forest, followed by mowed-closed, cultivated-mowed, mowed-grazed, and random grazed. Overall, grassland management practices that minimize soil disturbance, such as protected forest and mowed-closed, proved most effective in enhancing SOC storage and total N content. This study underscores the critical importance of implementing sustainable grassland management to maximize C sequestration in the region.","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"8 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70195","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inside Cover Picture 内页图片

IF 5.5 1区化学

Chinese Journal of Chemistry Pub Date : 2025-09-15 DOI: 10.1002/cjoc.70304

引用次数: 0

Whole-Genomic Characterization of Two Different PRRSV-1 Strains Isolated From a Single Pig 从一头猪分离的两种不同的PRRSV-1株的全基因组特征

IF 3 2区农林科学

Transboundary and Emerging Diseases Pub Date : 2025-09-15 DOI: 10.1155/tbed/8260067

Bangjun Gong, Jiahao Shi, Hu Xu, Chao Li, Lirun Xiang, Zhenyang Guo, Jinhao Li, Siyu Zhang, Zixuan Feng, Haonan Kang, Xueli Zhang, Ziyu Song, Qian Wang, Jinmei Peng, Guohui Zhou, Chaoliang Leng, Kuan Zhao, Yan-Dong Tang, Huairan Liu, Tong-Qing An, Xuehui Cai, Zhi-Jun Tian, Hongliang Zhang

{"title":"Whole-Genomic Characterization of Two Different PRRSV-1 Strains Isolated From a Single Pig","authors":"Bangjun Gong, Jiahao Shi, Hu Xu, Chao Li, Lirun Xiang, Zhenyang Guo, Jinhao Li, Siyu Zhang, Zixuan Feng, Haonan Kang, Xueli Zhang, Ziyu Song, Qian Wang, Jinmei Peng, Guohui Zhou, Chaoliang Leng, Kuan Zhao, Yan-Dong Tang, Huairan Liu, Tong-Qing An, Xuehui Cai, Zhi-Jun Tian, Hongliang Zhang","doi":"10.1155/tbed/8260067","DOIUrl":"https://doi.org/10.1155/tbed/8260067","url":null,"abstract":"Recent studies have demonstrated that porcine reproductive and respiratory syndrome virus 1 (PRRSV-1) strains isolated from different farms in China have significant genomic heterogeneity, whereas strains from the same farm present a high degree of genomic similarity. In this study, primary alveolar macrophages (PAMs) and high-throughput sequencing technology were used to successfully isolate and characterize two different PRRSV-1 strains (designated TZJ3556-1 and TZJ3556-2) in a single pig from a farm in Shandong Province with a PRRSV-1 outbreak. Phylogenetic analysis based on ORF5 and complete genome sequences revealed that both TZJ3556-1 and TZJ3556-2 belong to the BJEU06-1-like subgroup. Nucleotide (nt) sequence alignment revealed that the pairwise genomic similarity between TZJ3556-1 and TZJ3556-2 was only 86.03%. Additionally, both strains exhibited the characteristic 5 (4+1) discontinuous amino acid (aa) deletion in the Nsp2 region. Recombination analysis revealed that TZJ3556-1 is one of the parental strains for the recombinant strain TZJ3556-2, contributing Nsp1β, partial Nsp2, partial ORF4, and ORF5-3’UTR genes. This study reports, for the first time, coinfection with multiple PRRSV-1 strains in a single pig and the emergence of an intrasubgroup recombinant strain within the BJEU06-1-like subgroup. The genetic diversity of PRRSV-1 strains in China is likely to become increasingly complex, and these strains may have overcome physical barriers between farms. The emergence of complex and diverse PRRSV-1 strains poses a serious challenge for the prevention and control of PRRS in China.","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2025 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/8260067","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145057757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation of CD8⁺ T Cells in the Human Ex Vivo Lung Tumor Microenvironment Using Anti-CD3/CD28 and Nivolumab Anti-CD3/CD28和Nivolumab在人离体肺肿瘤微环境中激活CD8 + T细胞

IF 3.7 3区医学

European Journal of Immunology Pub Date : 2025-09-15 DOI: 10.1002/eji.70060

Tonia Bargmann, Sebastian Konzok, Renato Liguori, Maximilian Fuchs, Charline Sommer, Dirk Schaudien, Charlotte Schob, Stephan Halle, Christopher Werlein, Patrick Zardo, Lavinia Neubert, Danny Jonigk, Hans-Gerd Fieguth, Fulvia Ferrazzi, Katherina Sewald, Susann Dehmel, Armin Braun

{"title":"Activation of CD8⁺ T Cells in the Human Ex Vivo Lung Tumor Microenvironment Using Anti-CD3/CD28 and Nivolumab","authors":"Tonia Bargmann, Sebastian Konzok, Renato Liguori, Maximilian Fuchs, Charline Sommer, Dirk Schaudien, Charlotte Schob, Stephan Halle, Christopher Werlein, Patrick Zardo, Lavinia Neubert, Danny Jonigk, Hans-Gerd Fieguth, Fulvia Ferrazzi, Katherina Sewald, Susann Dehmel, Armin Braun","doi":"10.1002/eji.70060","DOIUrl":"https://doi.org/10.1002/eji.70060","url":null,"abstract":"Despite advancements in immunotherapies, the diversity of the tumor microenvironment remains a challenge for cancer treatment. To elucidate microenvironment-specific differences in antitumor responses, we established patient-derived ex vivo tumor-lung slices. We analyzed immune activation profiles after treatment with anti-CD3/CD28 and the checkpoint inhibitor Nivolumab. Lung slices from non-tumor, tumor-adjacent, tumor-border, and tumor-central tissue were generated and assessed for viability, cell composition, and immune competence via flow cytometry, soluble factor secretion, and bulk RNA-sequencing. The tumor-border contained the highest number of immune cells (8.3-fold vs. non-tumor), secreted tumor markers (S100 and CA15-3), and exhibited high levels of inflammatory mediators (IFNγ, IL-6, and IL-2). Treatment with anti-CD3/CD28 increased the frequency of CD137+/CD8+ T cells and induced cytokine responses dominated by IFNγ, IL-2, and Granzyme B. While both non-tumor and tumor-border tissue responded to anti-CD3/CD28, the intensities of immune responses were highly varied. Notably, treatment with Nivolumab induced an inflammatory response primarily in the tumor-border evidenced by IFNγ, IL-2, and Perforin secretion alongside increased expression of CD107a on CD8+ T cells, in a donor-dependent manner. Taken together, these data demonstrate how tumor-border tissue slices can be utilized to study T cell responses in the context of the patient-specific tumor microenvironment.","PeriodicalId":165,"journal":{"name":"European Journal of Immunology","volume":"55 9","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/eji.70060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient Expression and Purification of Recombinant Mouse Dimeric IgA 重组小鼠二聚体IgA的高效表达和纯化

IF 3.7 3区医学

European Journal of Immunology Pub Date : 2025-09-15 DOI: 10.1002/eji.70055

Antonia Geisse, Tao Zhang, Jonathan Schreiber, Kristina Markova, Sophie Burkhalter, Hedda Wardemann, Andrew J. Macpherson, Tim Rollenske

引用次数: 0

Exploring the Activities of Alginate-Carrageenan@AgNPs Hydrogels Supported by Molecular Docking Studies for Potential Antimicrobial and Antioxidant Agents 通过分子对接研究探索Alginate-Carrageenan@AgNPs水凝胶在潜在抗菌剂和抗氧化剂中的活性

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-09-15 DOI: 10.1002/biot.70117

Ghada E. Dawwam, Ahmed M. Khalil, Ahmed A. El-Rashedy, Doaa M. Azmy, Samir Kamel

{"title":"Exploring the Activities of Alginate-Carrageenan@AgNPs Hydrogels Supported by Molecular Docking Studies for Potential Antimicrobial and Antioxidant Agents","authors":"Ghada E. Dawwam, Ahmed M. Khalil, Ahmed A. El-Rashedy, Doaa M. Azmy, Samir Kamel","doi":"10.1002/biot.70117","DOIUrl":"https://doi.org/10.1002/biot.70117","url":null,"abstract":"<div>\u0000 \u0000 Alginates and kappa-carrageenan (KC) are natural anionic biopolymers widely utilized in food, pharmaceutical, and cosmetic industries for their anticoagulant, immunomodulatory, and antiviral properties. However, unmodified sodium alginate/KC (SA/KC) hydrogels often lack sufficient antimicrobial and antioxidant efficacy, limiting their functionality in advanced biomedical and packaging applications. To overcome these limitations, we designed a multifunctional hydrogel incorporating silver nanoparticles (AgNPs) into an ascorbic acid-crosslinked SA/KC matrix. Comprehensive physicochemical, morphological, antibacterial, antioxidant, and cytocompatibility assessments were conducted. The 2% AgNPs composite exhibited pronounced antimicrobial activity against Gram-positive (L. monocytogenes, S. aureus), Gram-negative (E. coli, Salmonella sp.), and fungal strain (Candida albicans). The 2% AgNPs hydrogel was cytocompatible with normal Vero cells (IC50 = 599.9 ± 3.47 µg/mL), while the 8% AgNPs variant demonstrated potent antioxidant capacity (IC50 of 21.77 µg/mL and 33.3 µg/mL via DPPH and ABTS assays, respectively). Molecular dynamics simulations confirmed strong interactions between hydrogel components and key binding site residues, ensuring composite stability. These results support the potential of AgNPs-enhanced SA/KC hydrogels for antimicrobial wound dressings, bioactive drug delivery matrices, and antioxidant-active food packaging materials.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 9","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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