{"title":"Nondestructive presymptomatic detection of blue-fluorescing substances in tomato leaves infected with <i>Ralstonia solanacearum</i> using a polyvinyl alcohol hydrogel.","authors":"Shigeyuki Iwasa, Yuso Kobara, Katsumi Maeda, Arina Asano, Tatsuya Shimanuki, Kuniaki Nagamine","doi":"10.1039/d5ay00579e","DOIUrl":"https://doi.org/10.1039/d5ay00579e","url":null,"abstract":"<p><p>This study proposes a nondestructive technique for the presymptomatic detection of pathogenic infections of plants, aiming to effectively prevent and control plant diseases in agriculture. The present and previous studies indicated that an increase in blue-fluorescing substances, including chlorogenic acid, in tomato leaves is a promising biomarker of infection with the pathogenic soil bacterium <i>Ralstonia solanacearum.</i> A soft and adhesive polyvinyl alcohol (PVA) hydrogel conformably adhered to the hydrophobic surface of the tomato leaf with a complex topography, mediated by non-volatile glycerol, enabling effective extraction of blue-fluorescing substances in a nondestructive manner. The fluorescence intensity of the PVA hydrogel increased a few days before the appearance of visible symptoms of bacterial wilt. This technique is expected to become a fundamental technology for the early detection of plant diseases.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Synergistic peptide-organic matrix enhances mineralization of biomimetic scaffolds for bone regeneration.","authors":"Yawen Huang, Ziqi Zhao, Yu Yang, Ruiqi Mao, Dongxuan Li, Fengxiong Luo, Kefeng Wang, Yujiang Fan, Xingdong Zhang","doi":"10.1039/d5mh00969c","DOIUrl":"https://doi.org/10.1039/d5mh00969c","url":null,"abstract":"<p><p>Biomimetic mineralized composites engineered <i>via</i> organic matrix templating show promise for bone repair but suffer from poor mineralization and imbalanced mechanical-biological performance. This study synergistically regulated biomolecules and organic matrix properties to enhance <i>in situ</i> mineralization, thereby improving mechanical strength and osteogenic potential. A nucleation-domain containing peptide (HGRGEAFDY) screened through molecular dynamics simulation was integrated into the gelatin matrix to prepare biomimetic materials with enhanced mineralization performance. The influence of peptide and organic matrix properties on mineralization capacity and saturation of <i>in situ</i> mineralization (SIM) was investigated. Results demonstrated that peptides with characteristic nucleation domains can boost mineralization by providing more nucleation sites and strengthening organic-inorganic interactions. Meanwhile, matrix compactness negatively correlated with mineralization capacity and SIM. Combined modulation of peptide nucleation ability and matrix compactness can enhance the SIM of the matrix, increasing the amount of minerals while improving mechanical properties. The biomimetic composites/scaffolds with mineralization enhancement by peptide-organic matrix regulation were evidenced to promote cell proliferation and osteogenic differentiation, and <i>in vivo</i> bone regeneration by upregulating BMP2 gene expression. This study provides valuable insights into the design of biomimetic mineralized materials and offers strategies for developing bone repair scaffolds with improved mechanical and biological performance.</p>","PeriodicalId":87,"journal":{"name":"Materials Horizons","volume":" ","pages":""},"PeriodicalIF":12.2,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Thermosensitive liposomal nanomedicine-functionalized photothermal composite scaffolds for light-guided cancer therapy.","authors":"Xiaohan Liu, Huajian Chen, Man Wang, Tianjiao Zeng, Toru Yoshitomi, Naoki Kawazoe, Yingnan Yang, Guoping Chen","doi":"10.1039/d5mh00888c","DOIUrl":"https://doi.org/10.1039/d5mh00888c","url":null,"abstract":"<p><p>In breast cancer treatment, the elimination of residual cancer cells in a sustainable and controllable manner to prevent recurrence remains a critical challenge in postoperative adjuvant therapy. In this study, a novel implantable <i>in situ</i> therapeutic composite scaffold platform (ALA@lipo/Au/Gel/PGA) was developed based on a porous scaffold composed of biocompatible gelatin and polyglutamic acid (PGA). This platform incorporated the photothermal agent Au nanorods (AuNRs) and thermosensitive liposomes encapsulated with the photosensitizer precursor 5-aminolevulinic acid (ALA). Due to the satisfactory photothermal conversion effect of the ALA@lipo/Au/Gel/PGA composite scaffold, the use of near-infrared (NIR) light not only ablated the cancer cells in the scaffold through photothermal therapy (PTT) but also induced the accelerated release of encapsulated ALA from the thermosensitive liposomes. After uptake, ALA could generate cytotoxic reactive oxygen species to increase tumour cell elimination efficiency <i>via</i> photodynamic therapy (PDT). Both <i>in vitro</i> and <i>in vivo</i> experiments demonstrated the synergistic anticancer effects of the composite scaffold. These results highlight the potential of this phototherapy-induced composite scaffold as a new synergistic treatment method for breast cancer.</p>","PeriodicalId":87,"journal":{"name":"Materials Horizons","volume":" ","pages":""},"PeriodicalIF":12.2,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Distinct impact of polystyrene microplastics on six species of common pathogenic and probiotic bacteria and their boosting support to <i>Vibrio cholerae</i> proliferation.","authors":"Tingting Gu, Yue Liu, Yongjie Wang, Huajun Zheng, Lanming Chen","doi":"10.1039/d5em00219b","DOIUrl":"https://doi.org/10.1039/d5em00219b","url":null,"abstract":"<p><p>Along with the widespread application of plastics, the release of polystyrene microplastics (PS-MPs) into the environment raises concerns about the risks of PS-MPs to human health. Hence, in this study, we investigated for the first time the impact of PS-MPs with different diameters on six species of common pathogenic and probiotic bacteria and further deciphered molecular mechanisms underlying the boosting support of PS-MPs for <i>Vibrio cholerae</i> proliferation. The results revealed that PS-MPs (100 nm to 5 μm, 0.1-100 mg L<sup>-1</sup>) promoted the reproduction of <i>V. cholerae</i>, but blocked the growth of <i>Vibrio parahaemolyticus</i>, <i>Klebsiella pneumoniae</i>, <i>Klebsiella oxytoca</i>, <i>Staphylococcus aureus</i>, and <i>Lactobacillus plantarum</i>, showing different size- and concentration-dependent action modes. The PS-MPs (100 nm to 5 μm, 100 mg L<sup>-1</sup>) significantly reduced the negative charge on the cell surface of <i>V. cholerae</i> and consequently increased the bacterial cell surface hydrophobicity and membrane fluidity, biofilm formation and self-aggregation capacity (<i>p</i> < 0.05). Comparative transcriptome analysis uncovered significantly up-regulated metabolic pathways in <i>V. cholerae</i> after exposure to the PS-MPs (100 nm, 100 mg L<sup>-1</sup>) for 8 h, leading to enhanced chemotaxis, flagellar motility, biofilm formation, energy metabolism and antioxidant defense. <i>In vitro</i> cell model experiments showed that the pre-exposure significantly increased the adhesion and invasion percentages of <i>V. cholerae</i> on human Caco-2 cells (<i>p</i> < 0.05). Overall, the results of this study not only fill the gaps in the interaction between the PS-MPs and common bacteria, but also highlight the amplified hazard of co-pollution by PS-MPs and <i>V. cholerae</i>, a leading waterborne pathogen worldwide.</p>","PeriodicalId":74,"journal":{"name":"Environmental Science: Processes & Impacts","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soft MatterPub Date : 2025-07-24DOI: 10.1039/d5sm00576k
Amrutha S V, Oliver Steinbock
{"title":"Co-motion of catalytic tubes and host droplets on superhydrophobic surfaces.","authors":"Amrutha S V, Oliver Steinbock","doi":"10.1039/d5sm00576k","DOIUrl":"https://doi.org/10.1039/d5sm00576k","url":null,"abstract":"<p><p>The ability to convert chemical energy into directed motion is a defining feature of living systems and a central goal in the design of synthetic active matter. Here, we report a self-propelling system in which a millimeter-sized tube is confined within a hydrogen peroxide droplet on a superhydrophobic surface. The tubes are synthesized <i>via</i> chemical garden self-assembly and catalyze the decomposition of the peroxide to water and oxygen gas. The resulting droplet-tube cell exhibits diverse dynamic behaviors, including propeller-like spinning, orbital rotation, and long-range translational jumps, driven by the asymmetric growth and bursting of internally generated gas bubbles. These motions are sensitive to hydrogen peroxide concentration, which governs both the internal gas production rate and the system's active lifetime. This system offers a simple yet versatile platform for exploring confined catalysis, emergent motility, and the design of soft, fuel-containing active materials.</p>","PeriodicalId":103,"journal":{"name":"Soft Matter","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Recent advances in the synthesis of multiple helicenes.","authors":"Zhen Chen, Wei-Chen Guo, Chuan-Feng Chen","doi":"10.1039/d5ob00966a","DOIUrl":"https://doi.org/10.1039/d5ob00966a","url":null,"abstract":"<p><p>Multiple helicenes, as helically twisted polycyclic frameworks with extended π-conjugated systems, have attracted growing attention due to their unique topologies, tunable chiroptical properties, and potential applications in chiral materials and organic electronics. In this review, we provide a systematic overview of the recent advances in the field of multiple helicenes and propose a concise classification strategy that classifies them into linear, cyclic, and cross-linked types. Based on this classification, we further summarize and highlight a range of efficient synthetic strategies that facilitate the construction of multiple helicenes. We hope this review will provide the design inspiration of multiple helicenes and further be helpful for the development of helicene chemistry.</p>","PeriodicalId":96,"journal":{"name":"Organic & Biomolecular Chemistry","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yangyang Hu, Chunlei Yu, Ya'nan Ran, Miao Yu, Jingwen Sun, Huiying Liu, Jiahui Zhang, Haijun Wang, Lei Liu
{"title":"Electrochemical [3 + 2] cycloaddition of anilines and enamine ketones: construction of 3-benzoylindole derivatives.","authors":"Yangyang Hu, Chunlei Yu, Ya'nan Ran, Miao Yu, Jingwen Sun, Huiying Liu, Jiahui Zhang, Haijun Wang, Lei Liu","doi":"10.1039/d5ob00831j","DOIUrl":"https://doi.org/10.1039/d5ob00831j","url":null,"abstract":"<p><p>This article describes an eco-friendly and atom-economical electrochemical protocol for the intermolecular [3 + 2] cycloaddition to synthesize 3-benzoylindole derivatives in synthetically viable yields. The oxidative cycloaddition proceeds under transition-metal-free and strong-oxidant-free conditions, generating H<sub>2</sub> and (Me)<sub>2</sub>NH as the byproducts, thereby aligning with green chemistry principles. Furthermore, the methodology exhibits exceptional synthetic versatility through successful late-stage diversification and compatibility with structurally diverse substrates. Preliminary cytotoxic evaluation <i>via</i> CCK-8 assays against two human carcinoma cell lines revealed potent bioactivity profiles, establishing a foundation for systematic exploration of indole-based pharmacophores. This protocol offers a sustainable alternative to conventional synthetic strategies while expanding the functionalization landscape of heteroaromatic systems.</p>","PeriodicalId":96,"journal":{"name":"Organic & Biomolecular Chemistry","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
RSC AdvancesPub Date : 2025-07-24DOI: 10.1039/D5RA00390C
Aiying Song, Rong Liu, Xinghe He and Linlin Wei
{"title":"Fabrication of robust polyimide-coated solid phase microextraction fibers for efficient extraction of fifteen polycyclic aromatic hydrocarbons from environmental water†","authors":"Aiying Song, Rong Liu, Xinghe He and Linlin Wei","doi":"10.1039/D5RA00390C","DOIUrl":"https://doi.org/10.1039/D5RA00390C","url":null,"abstract":"<p >A novel supporting material was developed by pulling melted glass onto stainless steel, providing a mechanically robust and chemically stable substrate. A polyimide-coated fiber for solid-phase microextraction (SPME) was then fabricated using a simple dipping method. The strong interactions between the polyimide coating and analytes, attributed to abundant unshared electron pairs in the p orbitals of O and N atoms, as well as π electrons in the C<img>O bond and benzene ring, contributed to the high extraction efficiency for sixteen polycyclic aromatic hydrocarbons. The developed polyimide-coated fiber-based SPME method, coupled with gas chromatography-flame ionization detection, exhibited low limits of detection (0.11–1.23 μg L<small><sup>−1</sup></small>) and a wide linear range (1–500 μg L<small><sup>−1</sup></small>) for polycyclic aromatic hydrocarbons in water samples. The method demonstrated excellent repeatability, with single-fiber repeatability ranging from 2.9% to 6.9% and fiber-to-fiber reproducibility between 3.9% and 11.5%. The recoveries of the analytes were between 82.7% and 107.2%. Additionally, the fiber exhibited outstanding durability, maintaining its extraction performance after more than 150 uses. The developed method was successfully applied for the determination of polycyclic aromatic hydrocarbons in river, pond, and sewage water samples, highlighting its potential as a sensitive, reliable, and cost-effective tool for environmental analysis.</p>","PeriodicalId":102,"journal":{"name":"RSC Advances","volume":" 15","pages":" 11826-11834"},"PeriodicalIF":3.9,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ra/d5ra00390c?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}