Cell structure and function最新文献_第4页

Reconstitution of functional tight junctions with individual claudin subtypes in epithelial cells. 用上皮细胞中的单个 claudin 亚型重建功能性紧密连接。

IF 1.5 4区生物学

Cell structure and function Pub Date : 2023-01-20 Epub Date: 2022-12-09 DOI: 10.1247/csf.22068

Mikio Furuse, Daiki Nakatsu, Wendy Hempstock, Shiori Sugioka, Noriko Ishizuka, Kyoko Furuse, Taichi Sugawara, Yugo Fukazawa, Hisayoshi Hayashi

{"title":"Reconstitution of functional tight junctions with individual claudin subtypes in epithelial cells.","authors":"Mikio Furuse, Daiki Nakatsu, Wendy Hempstock, Shiori Sugioka, Noriko Ishizuka, Kyoko Furuse, Taichi Sugawara, Yugo Fukazawa, Hisayoshi Hayashi","doi":"10.1247/csf.22068","DOIUrl":"10.1247/csf.22068","url":null,"abstract":"The claudin family of membrane proteins is responsible for the backbone structure and function of tight junctions (TJs), which regulate the paracellular permeability of epithelia. It is thought that each claudin subtype has its own unique function and the combination of expressed subtypes determines the permeability property of each epithelium. However, many issues remain unsolved in regard to claudin functions, including the detailed functional differences between claudin subtypes and the effect of the combinations of specific claudin subtypes on the structure and function of TJs. To address these issues, it would be useful to have a way of reconstituting TJs containing only the claudin subtype(s) of interest in epithelial cells. In this study, we attempted to reconstitute TJs of individual claudin subtypes in TJ-deficient MDCK cells, designated as claudin quinKO cells, which were previously established from MDCK II cells by deleting the genes of claudin-1, -2, -3, -4, and -7. Exogenous expression of each of claudin-1, -2, -3, -4, and -7 in claudin quinKO cells resulted in the reconstitution of functional TJs. These TJs did not contain claudin-12 and -16, which are endogenously expressed in claudin quinKO cells. Furthermore, overexpression of neither claudin-12 nor claudin-16 resulted in the reconstitution of TJs, demonstrating the existence of claudin subtypes lacking TJ-forming activity in epithelial cells. Exogenous expression of the channel-forming claudin-2, -10a, -10b, and -15 reconstituted TJs with reported paracellular channel properties, demonstrating that these claudin subtypes form paracellular channels by themselves without interaction with other subtypes. Thus, the reconstitution of TJs in claudin quinKO cells is advantageous for further investigation of claudin functions.Key words: tight junction, claudin, paracellular permeability, epithelial barrier.","PeriodicalId":9927,"journal":{"name":"Cell structure and function","volume":"48 1","pages":"1-17"},"PeriodicalIF":1.5,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10721951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9138393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Role of CRP2-MRTF interaction in functions of myofibroblasts. CRP2-MRTF 相互作用在肌成纤维细胞功能中的作用

IF 1.5 4区生物学

Cell structure and function Pub Date : 2023-01-01 DOI: 10.1247/csf.23004

Ken'ichiro Hayashi, Shinri Horoiwa, Kotaro Mori, Hiroshi Miyata, Reuben Jacob Labios, Tsuyoshi Morita, Yuka Kobayashi, Chiemi Yamashiro, Fumiaki Higashijima, Takuya Yoshimoto, Kazuhiro Kimura, Yoshiaki Nakagawa

{"title":"Role of CRP2-MRTF interaction in functions of myofibroblasts.","authors":"Ken'ichiro Hayashi, Shinri Horoiwa, Kotaro Mori, Hiroshi Miyata, Reuben Jacob Labios, Tsuyoshi Morita, Yuka Kobayashi, Chiemi Yamashiro, Fumiaki Higashijima, Takuya Yoshimoto, Kazuhiro Kimura, Yoshiaki Nakagawa","doi":"10.1247/csf.23004","DOIUrl":"10.1247/csf.23004","url":null,"abstract":"Inflammatory response induces phenotypic modulation of fibroblasts into myofibroblasts. Although transforming growth factor-βs (TGF-βs) evoke such transition, the details of the mechanism are still unknown. Here, we report that a LIM domain protein, cysteine-and glycine-rich protein 2 (CSRP2 [CRP2]) plays a vital role in the functional expression profile in myofibroblasts and cancer-associated fibroblasts (CAFs). Knock-down of CRP2 severely inhibits the expression of smooth muscle cell (SMC) genes, cell motility, and CAF-mediated collective invasion of epidermoid carcinoma. We elucidate the following molecular bases: CRP2 directly binds to myocardin-related transcription factors (MRTF-A/B [MRTFs]) and serum response factor (SRF) and stabilizes the MRTF/SRF/CArG-box complex to activate SMC gene expression. Furthermore, a three-dimensional structural analysis of CRP2 identifies the amino acids required for the CRP2-MRTF-A interaction. Polar amino acids in the C-terminal half (serine-152, glutamate-154, serine-155, threonine-156, threonine-157, and threonine-159 in human CRP2) are responsible for direct binding to MRTF-A. On the other hand, hydrophobic amino acids outside the consensus sequence of the LIM domain (tryptophan-139, phenylalanine-144, leucine-153, and leucine-158 in human CRP2) play a role in stabilizing the unique structure of the LIM domain.Key words: CRP2, 3D structure, myocardin-related transcription factor, myofibroblast, cancer-associated fibroblasts.","PeriodicalId":9927,"journal":{"name":"Cell structure and function","volume":"48 1","pages":"83-98"},"PeriodicalIF":1.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10721955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9837874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Significance of the p38MAPK-CRP2 axis in myofibroblastic phenotypic transition. p38MAPK-CRP2轴在肌成纤维细胞表型转变中的意义。

IF 2 4区生物学

Cell structure and function Pub Date : 2023-01-01 DOI: 10.1247/csf.23060

Ken'ichiro Hayashi, Reuben Jacob Labios, Tsuyoshi Morita, Atsushige Ashimori, Ren Aoki, Masanori Mikuni, Kazuhiro Kimura

{"title":"Significance of the p38MAPK-CRP2 axis in myofibroblastic phenotypic transition.","authors":"Ken'ichiro Hayashi, Reuben Jacob Labios, Tsuyoshi Morita, Atsushige Ashimori, Ren Aoki, Masanori Mikuni, Kazuhiro Kimura","doi":"10.1247/csf.23060","DOIUrl":"10.1247/csf.23060","url":null,"abstract":"We have recently demonstrated that a LIM domain protein, cysteine and glycine-rich protein 2 (CSRP2 [CRP2]), plays a vital role in the functional expression of myofibroblasts and cancer-associated fibroblasts. CRP2 binds directly to myocardin-related transcription factors (MRTF [MRTF-A or MRTF-B]) and serum response factor (SRF) to stabilize the MRTF/SRF/CArG-box complex, leading to the expression of smooth muscle cell (SMC) genes such as α-smooth muscle actin (α-SMA) and collagens. These are the marker genes for myofibroblasts. Here, we show that the adhesion of cultured human skin fibroblasts (HSFs) to collagen reduces the myofibroblastic features. HSF adhesion to collagen suppresses the expression of CRP2 and CSRP2-binding protein (CSRP2BP [CRP2BP]) and reduces the expression of SMC genes. Although CRP2BP is known as an epigenetic factor, we find that CRP2BP also acts as an adaptor protein to enhance the function of CRP2 mentioned above. This CRP2BP function does not depend on its histone acetyltransferase activity. We also addressed the molecular mechanism of the reduced myofibroblastic features of HSFs on collagen. HSF adhesion to collagen inhibits the p38MAPK-mediated pathway, and reducing the p38MAPK activity decreases the expression of CRP2 and SMC genes. Thus, the activation of p38MAPK is critical for the myofibroblastic features. We also show evidence that CRP2 plays a role in the myofibroblastic transition of retinal pigment epithelial cells (RPEs). Like HSFs, such phenotypic modulation of RPEs depends on the p38MAPK pathway.Key words: CRP2, p38MAPK, MRTF, myofibroblasts, retinal pigment epithelial cells.","PeriodicalId":9927,"journal":{"name":"Cell structure and function","volume":"48 2","pages":"199-210"},"PeriodicalIF":2.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11496777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71410928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative 3D correlative light and electron microscopy of organelle association during autophagy. 自噬过程中细胞器关联的定量三维关联光镜和电子显微镜。

IF 1.5 4区生物学

Cell structure and function Pub Date : 2022-12-22 Epub Date: 2022-11-22 DOI: 10.1247/csf.22071

Satoru Takahashi, Chieko Saito, Ikuko Koyama-Honda, Noboru Mizushima

{"title":"Quantitative 3D correlative light and electron microscopy of organelle association during autophagy.","authors":"Satoru Takahashi, Chieko Saito, Ikuko Koyama-Honda, Noboru Mizushima","doi":"10.1247/csf.22071","DOIUrl":"10.1247/csf.22071","url":null,"abstract":"In macroautophagy, disk-shaped double-membrane structures called phagophores elongate to form cup-shaped structures, becoming autophagosomes upon closure. These autophagosomes then fuse with lysosomes to become autolysosomes and degrade engulfed material. Autophagosome formation is reported to involve other organelles, including the endoplasmic reticulum (ER) and mitochondria. Organelles are also taken up by autophagosomes as autophagy cargos. However, few studies have performed systematic spatiotemporal analysis of inter-organelle relationships during macroautophagy. Here, we investigated the organelles in contact with phagophores, autophagosomes, and autolysosomes by using three-dimensional correlative light and electron microscopy with array tomography in cells starved 30 min. As previously reported, all phagophores associate with the ER. The surface area of phagophores in contact with the ER decreases gradually as they mature into autophagosomes and autolysosomes. However, the ER still associates with 92% of autophagosomes and 79% of autolysosomes, suggesting that most autophagosomes remain on the ER after closure and even when they fuse with lysosomes. In addition, we found that phagophores form frequently near other autophagic structures, suggesting the presence of potential hot spots for autophagosome formation. We also analyzed the contents of phagophores and autophagosomes and found that the ER is the most frequently engulfed organelle (detected in 65% of total phagophores and autophagosomes). These quantitative three-dimensional ultrastructural data provide insights into autophagosome-organelle relationships during macroautophagy.Key words: 3D-CLEM, autophagosome, electron microscopy, endoplasmic reticulum, lysosome.","PeriodicalId":9927,"journal":{"name":"Cell structure and function","volume":"47 2","pages":"89-99"},"PeriodicalIF":1.5,"publicationDate":"2022-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511054/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10767577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

PRL stimulates mitotic errors by suppressing kinetochore-localized activation of AMPK during mitosis. 在有丝分裂过程中，PRL 通过抑制着丝点定位的 AMPK 激活来刺激有丝分裂错误。

IF 1.5 4区生物学

Cell structure and function Pub Date : 2022-12-15 Epub Date: 2022-11-05 DOI: 10.1247/csf.22034

Kajung Ryu, Atsushi Yoshida, Yosuke Funato, Daisuke Yamazaki, Hiroaki Miki

{"title":"PRL stimulates mitotic errors by suppressing kinetochore-localized activation of AMPK during mitosis.","authors":"Kajung Ryu, Atsushi Yoshida, Yosuke Funato, Daisuke Yamazaki, Hiroaki Miki","doi":"10.1247/csf.22034","DOIUrl":"10.1247/csf.22034","url":null,"abstract":"Phosphatase of regenerating liver (PRL) is frequently overexpressed in various malignant cancers and is known to be a driver of malignancy. Here, we demonstrated that PRL overexpression causes mitotic errors that accompany spindle misorientation and aneuploidy, which are intimately associated with cancer progression. Mechanistic analyses of this phenomenon revealed dysregulation of the energy sensor kinase, AMP-activated protein kinase (AMPK), in PRL-induced mitotic errors. Specifically, immunofluorescence analysis showed that levels of phosphorylated AMPK (P-AMPK), an activated form of AMPK, at the kinetochore were reduced by PRL expression. Moreover, artificial activation of AMPK using chemical activators, such as A769662 and AICAR, in PRL-expressing cells restored P-AMPK signals at the kinetochore and normalized spindle orientation. Collectively, these results indicate the crucial importance of the activation of kinetochore-localized AMPK in the normal progression of mitosis, which is specifically perturbed by PRL overexpression.Key words: cancer, AMPK, PRL, kinetochore, mitotic errors.","PeriodicalId":9927,"journal":{"name":"Cell structure and function","volume":"47 2","pages":"75-87"},"PeriodicalIF":1.5,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10343344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activity patterns and burrowing ecology of the giant pouched rat (Cricetomys emini) in Tshuapa Province, D. R. Congo. 刚果（金）茨华帕省大袋鼠（Cricetomys emini）的活动模式和穴居生态学。

IF 0.8 4区生物学

Cell structure and function Pub Date : 2022-07-13 DOI: 10.1515/mammalia-2021-0197

Lem's N Kalemba, Clint N Morgan, Yoshinori J Nakazawa, Matthew R Mauldin, Jean M Malekani, Jeffrey B Doty

引用次数: 0

A method for the generation of pseudovirus particles bearing SARS coronavirus spike protein in high yields 一种高产产生带有SARS冠状病毒刺突蛋白的假病毒颗粒的方法

IF 1.5 4区生物学

Cell structure and function Pub Date : 2022-06-25 DOI: 10.1247/csf.21047

Yoichiro Fujioka, Sayaka Kashiwagi, Aiko Yoshida, Aya O. Satoh, Mari Fujioka, Maho Amano, Yohei Yamauchi, Yusuke Ohba

{"title":"A method for the generation of pseudovirus particles bearing SARS coronavirus spike protein in high yields","authors":"Yoichiro Fujioka, Sayaka Kashiwagi, Aiko Yoshida, Aya O. Satoh, Mari Fujioka, Maho Amano, Yohei Yamauchi, Yusuke Ohba","doi":"10.1247/csf.21047","DOIUrl":"https://doi.org/10.1247/csf.21047","url":null,"abstract":"The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has threatened human health and the global economy. Development of additional vaccines and therapeutics is urgently required, but such development with live virus must be conducted with biosafety level 3 confinement. Pseudotyped viruses have been widely adopted for studies of virus entry and pharmaceutical development to overcome this restriction. Here we describe a modified protocol to generate vesicular stomatitis virus (VSV) pseudotyped with SARS-CoV or SARS-CoV-2 spike protein in high yield. We found that a large proportion of pseudovirions produced with the conventional transient expression system lacked coronavirus spike protein at their surface as a result of inhibition of parental VSV infection by overexpression of this protein. Establishment of stable cell lines with an optimal expression level of coronavirus spike protein allowed the efficient production of progeny pseudoviruses decorated with spike protein. This improved VSV pseudovirus production method should facilitate studies of coronavirus entry and development of antiviral agents.","PeriodicalId":9927,"journal":{"name":"Cell structure and function","volume":"76 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138524174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Munc13b stimulus-dependently accumulates on granuphilin-mediated, docked granules prior to fusion. Munc13b刺激依赖性地在融合前积聚在颗粒蛋白介导的停靠颗粒上。

IF 1.5 4区生物学

Cell structure and function Pub Date : 2022-05-21 Epub Date: 2022-04-06 DOI: 10.1247/csf.22005

Kouichi Mizuno, Tetsuro Izumi

{"title":"Munc13b stimulus-dependently accumulates on granuphilin-mediated, docked granules prior to fusion.","authors":"Kouichi Mizuno, Tetsuro Izumi","doi":"10.1247/csf.22005","DOIUrl":"10.1247/csf.22005","url":null,"abstract":"The Rab27 effector granuphilin plays an indispensable role in stable docking of secretory granules to the plasma membrane by interacting with the complex of Munc18-1 and the fusion-incompetent, closed form of syntaxins-1~3. Although this process prevents spontaneous granule exocytosis, those docked granules actively fuse in parallel with other undocked granules after stimulation. Therefore, it is postulated that the closed form of syntaxins must be converted into the fusion-competent open form in a stimulus-dependent manner. Although Munc13 family proteins are generally thought to prime docked vesicles by facilitating conformational change in syntaxins, it is unknown which isoform acts in granuphilin-mediated, docked granule exocytosis. In the present study, we show that, although both Munc13a and Munc13b are expressed in mouse pancreatic islets and their beta-cell line MIN6, the silencing of Munc13b, but not that of Munc13a, severely affects glucose-induced insulin secretion. Furthermore, Munc13b accumulates on a subset of granules beneath the plasma membrane just prior to fusion during stimulation, whereas Munc13a is translocated to the plasma membrane where granules do not exist. When fluorescently labeled granuphilin was introduced to discriminate between molecularly docked granules and other undocked granules in living cells, Munc13b downregulation was observed to preferentially decrease the fusion of granuphilin-positive granules immobilized to the plasma membrane. These findings suggest that Munc13b promotes insulin exocytosis by clustering on molecularly docked granules in a stimulus-dependent manner.Key words: docking, insulin, live cell imaging, priming, TIRF microscopy.","PeriodicalId":9927,"journal":{"name":"Cell structure and function","volume":"1 1","pages":"31-41"},"PeriodicalIF":1.5,"publicationDate":"2022-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511056/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45709465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Specific association of TBK1 with the trans-Golgi network following STING stimulation. STING 刺激后，TBK1 与跨高尔基体网络的特异性关联。

IF 1.5 4区生物学

Cell structure and function Pub Date : 2022-03-08 Epub Date: 2022-02-05 DOI: 10.1247/csf.21080

Haruka Kemmoku, Yoshihiko Kuchitsu, Kojiro Mukai, Tomohiko Taguchi

{"title":"Specific association of TBK1 with the trans-Golgi network following STING stimulation.","authors":"Haruka Kemmoku, Yoshihiko Kuchitsu, Kojiro Mukai, Tomohiko Taguchi","doi":"10.1247/csf.21080","DOIUrl":"10.1247/csf.21080","url":null,"abstract":"Stimulator of interferon genes (STING) is essential for the type I interferon response induced by microbial DNA or self-DNA leaked from mitochondria/nuclei. In response to the emergence of such DNAs in the cytosol, STING relocates from the endoplasmic reticulum (ER) to the Golgi, and activates TANK-binding kinase 1 (TBK1), a cytosolic kinase essential for the activation of STING-dependent downstream signalling. To understand at which subcellular compartments TBK1 becomes associated with STING, we generated cells stably expressing fluorescent protein-tagged STING (mNeonGreen-STING) and TBK1 (TBK1-mScarletI). We found that after STING stimulation, TBK1 became associated with the trans-Golgi network (TGN), not the other parts of the Golgi. STING variants that constitutively induce the type I interferon response have been identified in patients with autoinflammatory diseases named \"STING-associated vasculopathy with onset in infancy (SAVI)\". Even in cells expressing these constitutively active STING variants, TBK1 was found to be associated with TGN, not the other parts of the Golgi. These results suggest that TGN acts as a specific platform where STING associates with and activates TBK1.Key words: the Golgi, membrane traffic, innate immunity, STING.","PeriodicalId":9927,"journal":{"name":"Cell structure and function","volume":"47 1","pages":"19-30"},"PeriodicalIF":1.5,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39893250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Ubiquitin-like 3 as a new protein-sorting factor for small extracellular vesicles. 类泛素 3 是一种新的细胞外小囊泡蛋白质分类因子。

IF 1.5 4区生物学

Cell structure and function Pub Date : 2022-01-01 DOI: 10.1247/csf.21078

Yusuke Takanashi, Tomoaki Kahyo, Sae Kamamoto, Hengsen Zhang, Bin Chen, Yashuang Ping, Kiyomichi Mizuno, Akikazu Kawase, Kei Koizumi, Masanori Satou, Kazuhito Funai, Norihiko Shiiya, Mitsutoshi Setou

{"title":"Ubiquitin-like 3 as a new protein-sorting factor for small extracellular vesicles.","authors":"Yusuke Takanashi, Tomoaki Kahyo, Sae Kamamoto, Hengsen Zhang, Bin Chen, Yashuang Ping, Kiyomichi Mizuno, Akikazu Kawase, Kei Koizumi, Masanori Satou, Kazuhito Funai, Norihiko Shiiya, Mitsutoshi Setou","doi":"10.1247/csf.21078","DOIUrl":"10.1247/csf.21078","url":null,"abstract":"Ubiquitin-like 3 (UBL3) is a well-conserved ubiquitin-like protein (UBL) in eukaryotes and regulates the ubiquitin cascade, but the significant roles of UBL3 in cellular processes remained unknown. Recently, UBL3 was elucidated to be a post-translational modification factor that promotes protein sorting to small extracellular vesicles (sEVs). Proteins sorted into sEVs have been studied as etiologies of sEV-related diseases. Also, there have been attempts to construct drug delivery systems (DDSs) by loading proteins into sEVs. In this review, we introduce the new concept that UBL3 has a critical role in the protein-sorting system and compare structure conservation between UBL3 and other UBLs from an evolutionary perspective. We conclude with future perspectives for the utility of UBL3 in sEV-related diseases and DDS.Key words: UBL3, small extracellular vesicles, protein sorting, ubiquitin-like protein, post-translational modification.","PeriodicalId":9927,"journal":{"name":"Cell structure and function","volume":"47 1","pages":"1-18"},"PeriodicalIF":1.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511055/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39658267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2