VAMP5 and distinct sets of cognate Q-SNAREs mediate exosome release.

IF 2 4区 生物学 Q4 CELL BIOLOGY
Cell structure and function Pub Date : 2023-10-27 Epub Date: 2023-09-14 DOI:10.1247/csf.23067
Takahide Matsui, Yuriko Sakamaki, Shu Hiragi, Mitsunori Fukuda
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引用次数: 0

Abstract

Small extracellular vesicles (sEVs) are largely classified into two types, plasma-membrane derived sEVs and endomembrane-derived sEVs. The latter type (referred to as exosomes herein) is originated from late endosomes or multivesicular bodies (MVBs). In order to release exosomes extracellularly, MVBs must fuse with the plasma membrane, not with lysosomes. In contrast to the mechanism responsible for MVB-lysosome fusion, the mechanism underlying the MVB-plasma membrane fusion is poorly understood. Here, we systematically analyze soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family proteins and identify VAMP5 as an MVB-localized SNARE protein required for exosome release. Depletion of VAMP5 in HeLa cells impairs exosome release. Mechanistically, VAMP5 mediates exosome release by interacting with SNAP47 and plasma membrane SNARE Syntaxin 1 (STX1) or STX4 to release exosomes. VAMP5 is also found to mediate asymmetric exosome release from polarized Madin-Darby canine kidney (MDCK) epithelial cells through interaction with the distinct sets of Q-SNAREs, suggesting that VAMP5 is a general exosome regulator in both polarized cells and non-polarized cells.Key words: exosome, small extracellular vesicle (sEV), multivesicular body, SNARE, VAMP5.

VAMP5和不同的同源Q-SNARE组介导外泌体释放。
细胞外小泡(sEV)主要分为两种类型,质膜衍生的sEV和内膜衍生的sVs。后一种类型(本文称为外泌体)起源于晚期内泌体或多泡体(MVBs)。为了在细胞外释放外泌体,MVBs必须与质膜融合,而不是与溶酶体融合。与MVB溶酶体融合的机制相反,MVB质膜融合的机制尚不清楚。在这里,我们系统地分析了可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体(SNARE)家族蛋白,并将VAMP5鉴定为外泌体释放所需的MVB定位SNARE蛋白。HeLa细胞中VAMP5的耗竭损害了外泌体的释放。从机制上讲,VAMP5通过与SNAP47和质膜SNARE突触融合蛋白1(STX1)或STX4相互作用来介导外泌体释放。VAMP5还被发现通过与不同组的Q-SNARE相互作用介导极化Madin-Darby犬肾(MDCK)上皮细胞的不对称外泌体释放,这表明VAMP5在极化细胞和非极化细胞中都是一种通用的外泌体调节因子。关键词:外泌体,细胞外小泡(sEV),多泡体,SNARE,VAMP5。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cell structure and function
Cell structure and function 生物-细胞生物学
CiteScore
2.50
自引率
0.00%
发文量
6
审稿时长
>12 weeks
期刊介绍: Cell Structure and Function is a fully peer-reviewed, fully Open Access journal. As the official English-language journal of the Japan Society for Cell Biology, it is published continuously online and biannually in print. Cell Structure and Function publishes important, original contributions in all areas of molecular and cell biology. The journal welcomes the submission of manuscripts on research areas such as the cell nucleus, chromosomes, and gene expression; the cytoskeleton and cell motility; cell adhesion and the extracellular matrix; cell growth, differentiation and death; signal transduction; the protein life cycle; membrane traffic; and organelles.
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