Cell structure and function最新文献

The expression of the formin Fhod3 in mouse tongue striated muscle. 形蛋白 Fhod3 在小鼠舌横纹肌中的表达。

IF 2 4区生物学

Cell structure and function Pub Date : 2024-11-07 Epub Date: 2024-10-10 DOI: 10.1247/csf.24044

Hikaru Nakagawa, Yohko Kage, Ayako Miura, Hikmawan Wahyu Sulistomo, Sho Matsuyama, Yoshihiro Yamashita, Ryu Takeya

{"title":"The expression of the formin Fhod3 in mouse tongue striated muscle.","authors":"Hikaru Nakagawa, Yohko Kage, Ayako Miura, Hikmawan Wahyu Sulistomo, Sho Matsuyama, Yoshihiro Yamashita, Ryu Takeya","doi":"10.1247/csf.24044","DOIUrl":"10.1247/csf.24044","url":null,"abstract":"The sarcomere is the contractile unit of striated muscle and is composed of actin and myosin filaments. There is increasing evidence to support that actin assembly mediated by Fhod3, a member of the formin family of proteins, is critical for sarcomere formation and maintenance in cardiac muscle. Fhod3, which is abundantly expressed in the heart, localizes to the center of sarcomeres and contributes to the regulation of the cardiac function, as evidenced by the fact that mutations in Fhod3 cause cardiomyopathy. However, the role of Fhod3 in skeletal muscle, another type of striated muscle, is unclear. We herein show that Fhod3 is expressed in the tongue at both mRNA and protein levels, although in smaller amounts than in the heart. To determine the physiological role of Fhod3 expressed in the tongue, we generated embryos lacking Fhod3 in the tongue. The tongue tissue of the Fhod3-depleted embryos did not show any significant structural defects, suggesting that Fhod3 is dispensable for normal development of the mouse tongue. Unexpectedly, the immunostaining analysis revealed the absence of specific sarcomeric signals for Fhod3 in the wild-type tongue when compared to the Fhod3-depleted tongue as a negative control, despite the use of antibodies that had previously been validated by immunostaining of heart tissues. Taken together, although Fhod3 protein is expressed at a significant level in the tongue, Fhod3 in the tongue does not appear to exhibit the same sarcomeric pattern as observed in the heart, suggesting a different role for Fhod3 in the tongue muscles.Key words: actin, formin, sarcomere, striated muscle.","PeriodicalId":9927,"journal":{"name":"Cell structure and function","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142388446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-wide arrangement of Golgi/RE units depends on the microtubule organization. 高尔基体/RE 单元在整个细胞内的排列取决于微管组织。

IF 2 4区生物学

Cell structure and function Pub Date : 2024-11-07 Epub Date: 2024-10-03 DOI: 10.1247/csf.24055

Tatsuya Tago, Syara Fujii, Shogo Sasaki, Maki Shirae-Kurabayashi, Naoaki Sakamoto, Takashi Yamamoto, Makoto Maeda, Tatsuya Ueki, Takunori Satoh, Akiko K Satoh

{"title":"Cell-wide arrangement of Golgi/RE units depends on the microtubule organization.","authors":"Tatsuya Tago, Syara Fujii, Shogo Sasaki, Maki Shirae-Kurabayashi, Naoaki Sakamoto, Takashi Yamamoto, Makoto Maeda, Tatsuya Ueki, Takunori Satoh, Akiko K Satoh","doi":"10.1247/csf.24055","DOIUrl":"10.1247/csf.24055","url":null,"abstract":"We have previously shown that Golgi stacks and recycling endosomes (REs) exist as Golgi/RE units in sea urchin embryos. In this study, we showed that Golgi/RE units were scattered throughout the cytoplasm at early developmental stages but gathered to form a \"Golgi ring\" surrounding the centric REs at the blastula stage. This change in the cell-wide arrangement of Golgi/RE units coincided with a dramatic change in microtubule organization from a randomly oriented cortical pattern to radial arrays under the apical plasma membrane. A single gigantic Golgi apparatus surrounding centric RE is clearly associated with the center of the radial microtubule arrays. Furthermore, we found that in some animal species belonging to different clades, Golgi stacks lack lateral connections but are likely centralized by microtubule motors. These results suggest that Golgi centralization depends on the organization of the microtubule array in addition to the lateral linking between Golgi stacks.Key words: Golgi stack, recycling endosome, Golgi-ribbon, microtubule, cilium, sea urchin, ascidian.","PeriodicalId":9927,"journal":{"name":"Cell structure and function","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142368182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular alterations associated with pathophysiology in liver-specific ZO-1 and ZO-2 knockout mice. 与肝特异性 ZO-1 和 ZO-2 基因敲除小鼠病理生理学相关的分子变化

IF 2 4区生物学

Cell structure and function Pub Date : 2024-10-26 Epub Date: 2024-09-26 DOI: 10.1247/csf.24046

Masahiko Itoh, Kenji Watanabe, Yoichi Mizukami, Hiroyuki Sugimoto

{"title":"Molecular alterations associated with pathophysiology in liver-specific ZO-1 and ZO-2 knockout mice.","authors":"Masahiko Itoh, Kenji Watanabe, Yoichi Mizukami, Hiroyuki Sugimoto","doi":"10.1247/csf.24046","DOIUrl":"10.1247/csf.24046","url":null,"abstract":"The liver is a complex organ with a highly organized structure in which tight junctions (TJs) play an important role in maintaining their function by regulating barrier properties and cellular polarity. Dysfunction of TJs is associated with liver diseases, including progressive familial intrahepatic cholestasis (PFIC). In this study, we investigated the molecular alterations in a liver-specific ZO-1 and ZO-2 double-knockout (DKO) mouse model, which exhibits features resembling those of PFIC4 patients with mutations in the ZO-2 gene. RNA-seq analysis revealed the upregulation of genes involved in the oxidative stress response, xenobiotic metabolism, and cholesterol metabolism in DKO livers. Conversely, the expression of genes regulated by HNF4α was lower in DKO livers than in the wild-type controls. Furthermore, age-associated analysis elucidated the timing and progression of these pathway changes as well as alterations in molecules related to TJs and apical polarity. Our research uncovered previously unknown implications of ZO-1 and ZO-2 in liver physiology and provides new insights into the molecular pathogenesis of PFIC4 and other tight junction-related liver diseases. These findings contribute to a better understanding of the complex mechanisms underlying liver function and dysfunction and may lead to the development of novel therapeutic strategies for liver diseases associated with tight junction impairment.Key words: tight junctions, ZO-1/ZO-2 knockout mouse, liver, transcriptome analysis, molecular pathological progression.","PeriodicalId":9927,"journal":{"name":"Cell structure and function","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142342647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visualization of ER-to-Golgi trafficking of procollagen X. 胶原蛋白 X 从 ER 到高尔基体运输的可视化。

IF 2 4区生物学

Cell structure and function Pub Date : 2024-10-02 Epub Date: 2024-09-06 DOI: 10.1247/csf.24024

Yuan Ximin, Hitoshi Hashimoto, Ikuo Wada, Nobuko Hosokawa

{"title":"Visualization of ER-to-Golgi trafficking of procollagen X.","authors":"Yuan Ximin, Hitoshi Hashimoto, Ikuo Wada, Nobuko Hosokawa","doi":"10.1247/csf.24024","DOIUrl":"10.1247/csf.24024","url":null,"abstract":"Collagen is the most abundant protein in the extracellular matrix of animals, and 28 types of collagen have been reported in humans. We previously analyzed the endoplasmic reticulum (ER)-to-Golgi transport of fibril-forming type III collagen (Hirata et al., 2022) and network-forming type IV collagen (Matsui et al., 2020), both of which have long collagenous triple-helical regions. To understand the ER-to-Golgi trafficking of various types of collagens, we analyzed the transport of short-chain type X collagen in this study. We fused cysteine-free GFP to the N-telopeptide region of procollagen X (GFP-COL10A1), as employed in our previous analysis of procollagens III and IV, and analyzed its transport by live-cell imaging. Procollagen X was transported to the Golgi apparatus via vesicular and tubular carriers containing ERGIC53 and RAB1B, similar to those used for procollagen III. Carriers containing procollagen X probably used the same transport processes as those containing conventional cargoes such as α1-antitrypsin. SAR1, TANGO1, SLY1/SCFD1, and BET3/TRAPPC3 were required for trafficking of procollagen X, which are different from the factors required for trafficking of procollagens III (SAR1, TANGO1, and CUL3) and IV (SAR1 and SLY1/SCFD1). These findings reveal that accommodation of various types of collagens with different shapes into carriers may require fine-tuning of the ER-to-Golgi transport machinery.Key words: collagen, GFP-procollagen X, ER-to-Golgi trafficking, export from ER, TANGO1.","PeriodicalId":9927,"journal":{"name":"Cell structure and function","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep learning-based segmentation of subcellular organelles in high-resolution phase-contrast images. 基于深度学习的高分辨率相位对比图像中亚细胞器的分割。

IF 2 4区生物学

Cell structure and function Pub Date : 2024-08-30 Epub Date: 2024-07-31 DOI: 10.1247/csf.24036

Kentaro Shimasaki, Yuko Okemoto-Nakamura, Kyoko Saito, Masayoshi Fukasawa, Kaoru Katoh, Kentaro Hanada

引用次数: 0

Deciphering the sub-Golgi localization of glycosyltransferases via 3D super-resolution imaging. 通过三维超分辨率成像解密糖基转移酶的亚高尔基定位。

IF 2 4区生物学

Cell structure and function Pub Date : 2024-08-27 Epub Date: 2024-08-22 DOI: 10.1247/csf.24008

Hirokazu Yagi, Seigo Tateo, Taiki Saito, Yusaku Ohta, Emiko Nishi, Saemi Obitsu, Tatsuya Suzuki, Supaphorn Seetaha, Charles Hellec, Akihiko Nakano, Takuro Tojima, Koichi Kato

{"title":"Deciphering the sub-Golgi localization of glycosyltransferases via 3D super-resolution imaging.","authors":"Hirokazu Yagi, Seigo Tateo, Taiki Saito, Yusaku Ohta, Emiko Nishi, Saemi Obitsu, Tatsuya Suzuki, Supaphorn Seetaha, Charles Hellec, Akihiko Nakano, Takuro Tojima, Koichi Kato","doi":"10.1247/csf.24008","DOIUrl":"10.1247/csf.24008","url":null,"abstract":"The Golgi apparatus, a crucial organelle involved in protein processing, including glycosylation, exhibits complex sub-structures, i.e., cis-, medial, and trans-cisternae. This study investigated the distribution of glycosyltransferases within the Golgi apparatus of mammalian cells via 3D super-resolution imaging. Focusing on human glycosyltransferases involved in N-glycan modification, we found that even enzymes presumed to coexist in the same Golgi compartment exhibit nuanced variations in localization. By artificially making their N-terminal regions [composed of a cytoplasmic, transmembrane, and stem segment (CTS)] identical, it was possible to enhance the degree of their colocalization, suggesting the decisive role of this region in determining the sub-Golgi localization of enzymes. Ultimately, this study reveals the molecular codes within CTS regions as key determinants of glycosyltransferase localization, providing insights into precise control over the positioning of glycosyltransferases, and consequently, the interactions between glycosyltransferases and substrate glycoproteins as cargoes in the secretory pathway. This study advances our understanding of Golgi organization and opens avenues for programming the glycosylation of proteins for clinical applications.Key words: Golgi apparatus, glycosyltransferase, 3D super-resolution imaging, N-glycosylation.","PeriodicalId":9927,"journal":{"name":"Cell structure and function","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reconstitution of nuclear envelope subdomain formation on mitotic chromosomes in semi-intact cells 半贴壁细胞有丝分裂染色体上核膜亚域形成的重建

IF 1.5 4区生物学

Cell structure and function Pub Date : 2024-06-04 DOI: 10.1247/csf.24003

Tomoko Funakoshi, Naoko Imamoto

{"title":"Reconstitution of nuclear envelope subdomain formation on mitotic chromosomes in semi-intact cells","authors":"Tomoko Funakoshi, Naoko Imamoto","doi":"10.1247/csf.24003","DOIUrl":"https://doi.org/10.1247/csf.24003","url":null,"abstract":"In metazoans, the nuclear envelope (NE) disassembles during the prophase and reassembles around segregated chromatids during the telophase. The process of NE formation has been extensively studied using live-cell imaging. At the early step of NE reassembly in human cells, specific pattern-like localization of inner nuclear membrane (INM) proteins, connected to the nuclear pore complex (NPC), was observed in the so-called “core” region and “noncore” region on telophase chromosomes, which corresponded to the “pore-free” region and the “pore-rich” region, respectively, in the early G1 interphase nucleus. We refer to these phenomena as NE subdomain formation. To biochemically investigate this process, we aimed to develop an in vitro NE reconstitution system using digitonin-permeabilized semi-intact mitotic human cells coexpressing two INM proteins, emerin and lamin B receptor, which were labeled with fluorescent proteins. The targeting and accumulation of INM proteins to chromosomes before and after anaphase onset in semi-intact cells were observed using time-lapse imaging. Our in vitro NE reconstitution system recapitulated the formation of the NE subdomain, as in living cells, although chromosome segregation and cytokinesis were not observed. This in vitro NE reconstitution required the addition of a mitotic cytosolic fraction supplemented with a cyclin-dependent kinase inhibitor and energy sources. The cytoplasmic soluble factor(s) dependency of INM protein targeting differed among the segregation states of chromosomes. Furthermore, the NE reconstituted on segregated chromosomes exhibited active nucleocytoplasmic transport competency. These results indicate that the chromosome status changes after anaphase onset for recruiting NPC components.","PeriodicalId":9927,"journal":{"name":"Cell structure and function","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141257791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A high-resolution phase-contrast microscopy system for label-free imaging in living cells 用于活细胞无标记成像的高分辨率相位对比显微系统

IF 1.5 4区生物学

Cell structure and function Pub Date : 2024-05-25 DOI: 10.1247/csf.24018

Kentaro Shimasaki, Yuko Okemoto-Nakamura, Saito Kyoko, Masayoshi Fukasawa, Kaoru Katoh, Kentaro Hanada

引用次数: 0

In vivo evidence for homeostatic regulation of ribosomal protein levels in Drosophila. 果蝇核糖体蛋白水平同态调节的体内证据

IF 2 4区生物学

Cell structure and function Pub Date : 2024-02-16 Epub Date: 2024-01-11 DOI: 10.1247/csf.23088

Daiki Kitamura, Kiichiro Taniguchi, Mai Nakamura, Tatsushi Igaki

{"title":"In vivo evidence for homeostatic regulation of ribosomal protein levels in Drosophila.","authors":"Daiki Kitamura, Kiichiro Taniguchi, Mai Nakamura, Tatsushi Igaki","doi":"10.1247/csf.23088","DOIUrl":"10.1247/csf.23088","url":null,"abstract":"The ribosome is a molecular machine essential for protein synthesis, which is composed of approximately 80 different ribosomal proteins (Rps). Studies in yeast and cell culture systems have revealed that the intracellular level of Rps is finely regulated by negative feedback mechanisms or ubiquitin-proteasome system, which prevents over- or under-abundance of Rps in the cell. However, in vivo evidence for the homeostatic regulation of intracellular Rp levels has been poor. Here, using Drosophila genetics, we show that intracellular Rp levels are regulated by proteasomal degradation of excess Rps that are not incorporated into the ribosome. By establishing an EGFP-fused Rp gene system that can monitor endogenously expressed Rp levels, we found that endogenously expressed EGFP-RpS20 or -RpL5 is eliminated from the cell when RpS20 or RpL5 is exogenously expressed. Notably, the level of endogenously expressed Hsp83, a housekeeping gene, was not affected by exogenous expression of Hsp83, suggesting that the strict negative regulation of excess protein is specific for intracellular Rps. Further analyses revealed that the maintenance of cellular Rp levels is not regulated at the transcriptional level but by proteasomal degradation of excess free Rps as a protein quality control mechanism. Our observations provide not only the in vivo evidence for the homeostatic regulation of Rp levels but also a novel genetic strategy to study in vivo regulation of intracellular Rp levels and its role in tissue homeostasis via cell competition.Key words: ribosomal protein, proteasomal degradation, Drosophila.","PeriodicalId":9927,"journal":{"name":"Cell structure and function","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11496781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139416479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coronin-1 promotes directional cell rearrangement in Drosophila wing epithelium. 冠状蛋白-1促进果蝇翅膀上皮细胞定向重排。

IF 2 4区生物学

Cell structure and function Pub Date : 2023-12-20 Epub Date: 2023-11-30 DOI: 10.1247/csf.23049

Keisuke Ikawa, Souta Hiro, Shu Kondo, Shizue Ohsawa, Kaoru Sugimura

{"title":"Coronin-1 promotes directional cell rearrangement in Drosophila wing epithelium.","authors":"Keisuke Ikawa, Souta Hiro, Shu Kondo, Shizue Ohsawa, Kaoru Sugimura","doi":"10.1247/csf.23049","DOIUrl":"10.1247/csf.23049","url":null,"abstract":"Directional cell rearrangement is a critical process underlying correct tissue deformation during morphogenesis. Although the involvement of F-actin regulation in cell rearrangement has been established, the role and regulation of actin binding proteins (ABPs) in this process are not well understood. In this study, we investigated the function of Coronin-1, a WD-repeat actin-binding protein, in controlling directional cell rearrangement in the Drosophila pupal wing. Transgenic flies expressing Coronin-1-EGFP were generated using CRISPR-Cas9. We observed that Coronin-1 localizes at the reconnecting junction during cell rearrangement, which is dependent on actin interacting protein 1 (AIP1) and cofilin, actin disassemblers and known regulators of wing cell rearrangement. Loss of Coronin-1 function reduces cell rearrangement directionality and hexagonal cell fraction. These results suggest that Coronin-1 promotes directional cell rearrangement via its interaction with AIP1 and cofilin, highlighting the role of ABPs in the complex process of morphogenesis.Key words: morphogenesis, cell rearrangement, actin binding proteins (ABPs).","PeriodicalId":9927,"journal":{"name":"Cell structure and function","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11496784/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138458219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0