Proteomic analysis of fatty liver induced by starvation of medaka fish larvae.

IF 2 4区 生物学 Q4 CELL BIOLOGY
Cell structure and function Pub Date : 2023-07-19 Epub Date: 2023-06-28 DOI:10.1247/csf.23014
Tomoyo Ikeda, Tokiro Ishikawa, Satoshi Ninagawa, Tetsuya Okada, Masaya Ono, Kazutoshi Mori
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引用次数: 0

Abstract

When medaka fish (Oryzias latipes) larvae are grown in the absence of exogenous nutrition, the liver becomes dark and positive to Oil Red O staining from 7 days post-hatch (dph). We determined the mechanism of this starvation-induced development of fatty liver by proteomic analysis using livers obtained from larvae grown in the presence or absence of 2% glucose at 5 dph. Results showed that changes in the expression levels of enzymes involved in glycolysis or the tricarboxylic acid cycle were modest, whereas the expression levels of enzymes involved in amino acid catabolism or β-oxidation of fatty acids were significantly elevated, suggesting that they become major energy sources under starvation conditions. Expression levels of enzymes for the uptake and β-oxidation of fatty acids as well as synthesis of triacylglycerol were elevated, whereas those for the synthesis of cholesterol as well as export of cholesterol and triacylglycerol were decreased under starvation conditions, which explains the accumulation of triacylglycerol in the liver. Our results provide the basis for future research to understand how gene malfunction(s) affects the development of fatty liver, which can lead to nonalcoholic steatohepatitis and then to liver cirrhosis.Key words: amino acid catabolism, β-oxidation, triacylglycerol, cholesterol, export.

青鳉幼鱼饥饿诱发脂肪肝的蛋白质组分析
当青鱼(Oryzias latipes)幼体在缺乏外源营养的情况下生长时,从孵化后 7 天(dph)开始,肝脏颜色变深,油红 O 染色呈阳性。我们利用在有或没有 2% 葡萄糖的条件下生长的幼虫的肝脏,通过蛋白质组分析确定了饥饿诱导脂肪肝形成的机制。结果表明,参与糖酵解或三羧酸循环的酶的表达水平变化不大,而参与氨基酸分解代谢或脂肪酸β-氧化的酶的表达水平显著升高,这表明它们在饥饿条件下成为主要的能量来源。在饥饿条件下,脂肪酸的摄取和β-氧化以及三酰甘油合成酶的表达水平升高,而胆固醇的合成以及胆固醇和三酰甘油的输出酶的表达水平降低,这解释了三酰甘油在肝脏中积累的原因。我们的研究结果为今后了解基因功能失调如何影响脂肪肝的发展,进而导致非酒精性脂肪性肝炎和肝硬化的研究提供了依据。
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来源期刊
Cell structure and function
Cell structure and function 生物-细胞生物学
CiteScore
2.50
自引率
0.00%
发文量
6
审稿时长
>12 weeks
期刊介绍: Cell Structure and Function is a fully peer-reviewed, fully Open Access journal. As the official English-language journal of the Japan Society for Cell Biology, it is published continuously online and biannually in print. Cell Structure and Function publishes important, original contributions in all areas of molecular and cell biology. The journal welcomes the submission of manuscripts on research areas such as the cell nucleus, chromosomes, and gene expression; the cytoskeleton and cell motility; cell adhesion and the extracellular matrix; cell growth, differentiation and death; signal transduction; the protein life cycle; membrane traffic; and organelles.
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