Chemistry Central Journal最新文献

{"title":"Antioxidant activity of polyphenolic compounds isolated from ethyl-acetate fraction of Acacia hydaspica R. Parker.","authors":"Tayyaba Afsar,&nbsp;Suhail Razak,&nbsp;Maria Shabbir,&nbsp;Muhammad Rashid Khan","doi":"10.1186/s13065-018-0373-x","DOIUrl":"https://doi.org/10.1186/s13065-018-0373-x","url":null,"abstract":"<p><strong>Background: </strong>Acacia hydaspica belongs to family leguminosae possess antioxidant, anti-inflammatory and anticancer activities. During our search for antioxidant compounds from A. hydaspica, we carried out bioassay guided fractionation and obtained antioxidant compounds with free radical scavenging activity.</p><p><strong>Materials and methods: </strong>The polyphenol compounds in the plant extract of A. hydaspica were isolated by combination of different chromatographic techniques involving vacuum liquid chromatography and medium pressure liquid chromatography. The structural heterogeneity of isolated compounds was characterized by high pressure liquid chromatography, MS-ESI and NMR spectroscopic analyses. The antioxidant potential of isolated compounds has been investigated by 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide scavenging potential, hydroxyl radical scavenging potential, ferric reducing/antioxidant power (FRAP) model systems and total antioxidant capacity measurement.</p><p><strong>Results: </strong>The isolated compounds show the predominance of signals representative of 7-O-galloyl catechins, catechins and methyl gallate. Flash chromatographic separation gives 750 mg of 7-O galloyl catechin, 400 mg of catechin and 150 mg of methyl gallate from 4 g loaded fraction on ISCO. Results revealed that C1 was the most potent compound against DPPH (EC₅₀ 1.60 ± 0.035 µM), nitric oxide radical (EC₅₀ 6 ± 0.346 µM), showed highest antioxidant index (1.710 ± 0.04) and FRAP [649.5 ± 1.5 µM Fe(II)/g] potency at 12.5 µM dose compared to C2, C3 and standard reference, whereas C3 showed lower EC₅₀ values (4.33 ± 0.618 µM) in OH radical scavenging assay.</p><p><strong>Conclusion: </strong>Present research reports for the first time the antioxidant activity of polyphenolic compounds of A. hydaspica. Result showed good resolution and separation from other constituents of extract and method was found to be simple and precise. The isolation of catechin from this new species could provide a varied opportunity to obtain large quantities of catechin and catechin isomers beside from green tea. Free radical scavenging properties of isolated catechin isomers from A. hydaspica merit further investigations for consumption of this plant in oxidative stress related disorders.</p>","PeriodicalId":9842,"journal":{"name":"Chemistry Central Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s13065-018-0373-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35769452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of flutamide and two major metabolites using HPLC-DAD and HPTLC methods.","authors":"Nada S Abdelwahab, Heba A H Elshemy, Nehal F Farid","doi":"10.1186/s13065-018-0372-y","DOIUrl":"10.1186/s13065-018-0372-y","url":null,"abstract":"<p><p>Flutamide is a potential antineoplastic drug classified as an anti-androgen. It is a therapy for men with advanced prostate cancer, administered orally after which it undergoes extensively first pass metabolism in the liver with the production of several metabolites. These metabolites are predominantly excreted in urine. One of the important metabolites in plasma is 4-nitro-3-(trifluoromethyl)phenylamine (Flu-1), while the main metabolite in urine is 2-amino-5-nitro-4-(trifluoromethyl)phenol (Flu-3). In this work the two metabolites, Flu-1 and Flu-3, have been synthesized, and then structural confirmation has been carried out by HNMR analysis. Efforts were exerted to develop chromatographic methods for resolving Flutamide and its metabolites with the use of acceptable solvents without affecting the efficiency of the methods. The drug along with its metabolites were quantitatively analyzed in pure form, human urine, and plasma samples using two chromatographic methods, HPTLC and HPLC-DAD methods. FDA guidelines for bio-analytical method validation were followed and USP recommendations were used for analytical method validation. Interference from excipients has been tested by application of the methods to pharmaceutical tablets. No significant difference was found between the proposed methods and the official one when they were statistically compared at p value of 0.05%.</p>","PeriodicalId":9842,"journal":{"name":"Chemistry Central Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35768314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening for cytotoxic chemical constituents from Justicia procumbens by HPLC-DAD-ESI-MS and NMR.","authors":"Bo Liu,&nbsp;Yanfang Yang,&nbsp;Hongbin Liu,&nbsp;Zhoutao Xie,&nbsp;Qun Li,&nbsp;Meng Deng,&nbsp;Fangping Li,&nbsp;Jingling Peng,&nbsp;Hezhen Wu","doi":"10.1186/s13065-018-0371-z","DOIUrl":"https://doi.org/10.1186/s13065-018-0371-z","url":null,"abstract":"<p><strong>Background: </strong>The Acanthaceae family is an important source of therapeutic drugs and ethno medicines. There are many famous medicinal plants from this family, such as Andrographis paniculata, Baphicacanthus cusia, and Dicliptera chinensis. Justicia procumbens (J. procumbens) is widely distributed in tropical and sub-tropical of the world. It has long been used in traditional Chinese medicine for cancer. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed the ethyl acetate extract of J. procumbens had a cytotoxic activity. Therefore, qualitative and quantitative analysis of the chemical constituents in the ethyl acetate extract was important for understanding its pharmacological mechanism.</p><p><strong>Results: </strong>A high-performance liquid chromatography with diode array detection coupled to electrospray ionisation quadrupole time-of-flight tandem mass spectrometry procedure was established. Eleven dibenzylbutanes and four arylnaphthalenes were confirmed by HPLC-DAD-ESI-QTOF-MS analysis. A novel dibenzylbutane (5-methoxy-4,4'-di-O-methylsecolariciresinol-9'-monoacetate) and seven isomers of arylnaphthalene were isolated and characterized by NMR and QTOF-MS. Compounds 1, 2, and 13 were detected for the first time. The content of six lignans were determinated in the ethyl acetate extract.</p><p><strong>Conclusions: </strong>This study showed that the cytotoxic activity assay of J. procumbens could be mainly attributed to the constituents of lignans. The bioactivity of the ethyl acetate extract and determined compounds support the traditional use of this plant in cancer. These chemical constituents may be developed as novel therapeutics.</p>","PeriodicalId":9842,"journal":{"name":"Chemistry Central Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s13065-018-0371-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35769439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutagenicity, cytotoxic and antioxidant activities of Ricinus communis different parts.","authors":"Mazhar Abbas, Abid Ali, Muhammad Arshad, Asia Atta, Zahed Mehmood, Imtiaz Mahmood Tahir, Munawar Iqbal","doi":"10.1186/s13065-018-0370-0","DOIUrl":"10.1186/s13065-018-0370-0","url":null,"abstract":"<p><p>Ricinus communis (castor plant) is a potent medicinal plant, which is commonly used in the treatment of various ailments. The present study was conducted to appraise the cytotoxicity and mutagenicity of R. communis along with antioxidant and antimicrobial activities. Cytotoxicity was evaluated by hemolytic and brine shrimp assays, whereas Ames test (TA98 and TA100) was used for mutagenicity evaluation. Plant different parts were extracted in methanol by shaking, sonication and Soxhlet extraction methods. The R. communis methanolic extracts showed promising antioxidant activity evaluated as through total phenolic contents (TPC), total flavonoid content (TFC), DPPH free radical inhibition, reducing power and inhibition of linoleic acid oxidation. R. communis seeds, stem, leaves, fruit and root methanolic extracts showed mild to moderate cytotoxicity against red blood cells (RBCs) of human and bovine. Brine shrimp lethality also revealed the cytotoxic nature of extracts with LC₅₀ in the range of 0.22-3.70 (µg/mL) (shaking), 1.59-60.92 (µg/mL) (sonication) and 0.72-33.60 (µg/mL) (Soxhlet), whereas LC₉₀ values were in the range of 345.42-1695.81, 660.50-14,794.40 and 641.62-15,047.80 µg/mL for shaking, sonication and Soxhlet extraction methods, respectively. R. communis methanolic extracts revealed mild mutagenicity against TA98 (range 1975 ± 67 to 2628 ± 79 revertant colonies) and TA100 (range 2773 ± 92 to 3461 ± 147 revertant colonies) strains and these values were 3267 ± 278 and 4720 ± 346 revertant colonies in case of TA98 and TA100 positive controls, respectively. R. communis methanolic extracts prevented the H₂O₂ and UV to Plasmid pBR³²² DNA oxidative damage. Results revealed that R. communis is a potential source of bioactive compounds and in future studies the bioactive compounds will be identified by advanced spectroscopic techniques.</p>","PeriodicalId":9842,"journal":{"name":"Chemistry Central Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5775190/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35750764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of water quality and human risk assessment due to heavy metals in groundwater around Muledane area of Vhembe District, Limpopo Province, South Africa.","authors":"Joshua Nosa Edokpayi,&nbsp;Abimbola Motunrayo Enitan,&nbsp;Ntwanano Mutileni,&nbsp;John Ogony Odiyo","doi":"10.1186/s13065-017-0369-y","DOIUrl":"https://doi.org/10.1186/s13065-017-0369-y","url":null,"abstract":"<p><p>Groundwater is considered as good alternative to potable water because of its low turbidity and perceived low contamination. The study assessed the physio-chemical and heavy metals concentrations in eight randomly selected boreholes water at Muledane village in Limpopo Province of South Africa and the results were compared with South African National standard permissible limit. The impacts of heavy metals on human health was further determined by performing quantitative risk assessment through ingestion and dermal adsorption of heavy metals separately for adults and children in order to estimate the magnitude of heavy metals in the borehole samples. Parameters such as turbidity, nitrate, iron, manganese and chromium in some investigated boreholes did not comply with standard limits sets for domestic water use. Multivariate analyses using principal component analysis and hierarchical cluster analysis revealed natural and anthropogenic activities as sources of heavy metal contamination in the borehole water samples. The calculated non-carcinogenic effects using hazard quotient toxicity potential, cumulative hazard index and chronic daily intake of groundwater through ingestion and dermal adsorption pathways were less than a unity, which showed that consumption of the water could pose little or no significant health risk. However, maximum estimated values for an individual exceeded the risk limit of 10^-6 and 10^-4 with the highest estimated carcinogenic exposure risk (CR_ing) for Cr and Pb in the groundwater. This could pose potential health risk to both adults and children in the investigated area. Therefore, precaution needs to be taken to avoid potential CR_ing of people in Muledane area especially, children using the borehole water.</p>","PeriodicalId":9842,"journal":{"name":"Chemistry Central Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s13065-017-0369-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35730834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, anti-microbial activity, cytotoxicity of some novel substituted (5-(3-(1H-benzo[d]imidazol-2-yl)-4-hydroxybenzyl)benzofuran-2-yl)(phenyl)methanone analogs.","authors":"Bhookya Shankar,&nbsp;Pochampally Jalapathi,&nbsp;Balabadra Saikrishna,&nbsp;Shaym Perugu,&nbsp;Vijjulatha Manga","doi":"10.1186/s13065-017-0364-3","DOIUrl":"https://doi.org/10.1186/s13065-017-0364-3","url":null,"abstract":"<p><strong>Background: </strong>There is a dire need for the discovery and development of new antimicrobial agents after several experiments for a better resistance of microorganisms towards antimicrobial agents become a serious health problem for a few years in the past. As benzimidazole possess various types of biological activities, it has been synthesized, in the present study, a new series of (5-(3-(1H-benzo[d]imidazol-2-yl)-4-hydroxybenzyl)benzofuran-2-yl)(phenyl)methanone analogs by using the condensation and screened for its in vitro antimicrobial activity and cytotoxicity.</p><p><strong>Results: </strong>The synthesized (5-(3-(1H-benzo[d]imidazol-2-yl)-4-hydroxybenzyl) benzofuran-2-yl)(phenyl)methanone analogs were confirmed by IR, ¹H and ¹³C-NMR, MS spectra and HRMS spectral data. The synthesized compounds were evaluated for their in vitro antimicrobial potential against Gram-positive (Bacillus subtilis, Bacillus megaterium, Staph aureus and Streptococcus pyogenes), Gram-negative (Escherichia coli, Proteus vulgaris, Proteus mirabilis and Enterobacter aerogenes) bacterial and fungal (Aspergillus niger, Candida albicans, Fusarium oxysporum, Fusarium solani) strains by disc diffusion method and the minimum inhibitory concentration (MIC) in which it has been recorded in microgram per milliliter in comparison to the reference drugs, ciprofloxacin (antibacterial) and nystatin (antifungal). Further, the cytotoxicity (IC₅₀ value) has also been assessed on human cervical (HeLa), Supt1 cancer cell lines by using MTT assay.</p><p><strong>Conclusions: </strong>The following screened compounds (4d), (4f), (4g), (4k), (4l), (4o) and (4u) were found to be the best active against all the tested bacterial and fungal strains among all the demonstrated compounds of biological study. The MIC determination was also carried out against bacteria and fungi, the compounds (4f) and (4u) are found to be exhibited excellent potent against bacteria and fungi respectively. The compounds (4f) and (4u) were shown non-toxic in nature after screened for cytotoxicity against the cancer cell lines of human cervical (HeLa) and Supt1. Additionally, structure and antibacterial activity relationship were also further supported by in silico molecular docking studies of the active compounds against DNA topoisomerase.</p>","PeriodicalId":9842,"journal":{"name":"Chemistry Central Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s13065-017-0364-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35724160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel pyridine-based Pd(II)-complex for efficient Suzuki coupling of aryl halides under microwaves irradiation in water.","authors":"Ismail I Althagafi,&nbsp;Mohamed R Shaaban,&nbsp;Aisha Y Al-Dawood,&nbsp;Ahmad M Farag","doi":"10.1186/s13065-017-0320-2","DOIUrl":"https://doi.org/10.1186/s13065-017-0320-2","url":null,"abstract":"<p><p>Suzuki C-C cross-coupling of aryl halides with aryl boronic acids using new phosphene-free palladium complexes as precatalysts was investigated. A pyridine-based Pd(II)-complex was used in open air under thermal as well as microwave irradiation conditions using water as an eco-friendly green solvent.</p>","PeriodicalId":9842,"journal":{"name":"Chemistry Central Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s13065-017-0320-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35507673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formation of amides: one-pot condensation of carboxylic acids and amines mediated by TiCl₄.","authors":"Antonella Leggio,&nbsp;Jessica Bagalà,&nbsp;Emilia Lucia Belsito,&nbsp;Alessandra Comandè,&nbsp;Marianna Greco,&nbsp;Angelo Liguori","doi":"10.1186/s13065-017-0318-9","DOIUrl":"https://doi.org/10.1186/s13065-017-0318-9","url":null,"abstract":"<p><p>A general procedure for the synthesis of amides via the direct condensation of carboxylic acids and amines in the presence of TiCl₄ is reported. The amidation reaction was performed in pyridine at 85 °C with a wide range of substrates providing the corresponding amide products in moderate to excellent yields and high purity. The reaction proceeds with low yields when both the carboxylic acid and the amine are sterically hindered. The process takes place with nearly complete preservation of the stereochemical integrity of chiral substrates.</p>","PeriodicalId":9842,"journal":{"name":"Chemistry Central Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s13065-017-0318-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35507674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The development of an effective synthetic route of lesinurad (RDEA594).","authors":"Qing Meng,&nbsp;Tong Zhao,&nbsp;Dongwei Kang,&nbsp;Boshi Huang,&nbsp;Peng Zhan,&nbsp;Xinyong Liu","doi":"10.1186/s13065-017-0316-y","DOIUrl":"https://doi.org/10.1186/s13065-017-0316-y","url":null,"abstract":"<p><strong>Background: </strong>Lesinurad is a novel selective uric acid salt transport protein 1 (URAT1) inhibitor which is approved in the USA for the treatment of gout. However, there are some shortcomings among the reported synthetic routes, such as expensive materials, environmental pollution and poor yield.</p><p><strong>Results: </strong>In this study, an efficient, practical and environmentally-friendly synthetic route of lesinurad is reported. The main advantages of this route include inexpensive starting materials, mild conditions and acceptable overall yield (38.8%).</p><p><strong>Conclusion: </strong>Generally, this procedure is reasonable, reliable and suitable for industrial production. Graphical abstract The improved synthetic procedure of lesinurad (I).</p>","PeriodicalId":9842,"journal":{"name":"Chemistry Central Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s13065-017-0316-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35561064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, characterization, anti-ulcer action and molecular docking evaluation of novel benzimidazole-pyrazole hybrids.","authors":"Abida Noor, Neelum Gul Qazi, Humaira Nadeem, Arif-Ullah Khan, Rehan Zafar Paracha, Fawad Ali, Adil Saeed","doi":"10.1186/s13065-017-0314-0","DOIUrl":"10.1186/s13065-017-0314-0","url":null,"abstract":"<p><p>A series of six novel benzimidazole-pyrazole hybrid molecules was synthesized and characterized using elemental analysis (CHN) and spectroscopic methods (¹HNMR, FT-IR). All the synthesized compounds were evaluated for their in vivo anti ulcerogenic activity using Albino rats (weighing 180-220 g). The interactions between the compounds and active site residues of H⁺/K⁺ ATPase were investigated by molecular docking studies using autodock vina 4.0. SCH28080 was used to validate the docking results. Also the drug likeliness of these compounds was predicted using Molinspiration server in light of Lipinski's rule of five. All the six synthesized compounds exhibited higher anti-ulcer activity as compared to omeprazole. These novel hybrid compounds showed comparable anti-ulcer potential of 72-83% at dose level of 500 µg/kg, whereas omeprazole showed 83% anti-ulcer activity at dose level of 30 mg/kg. The results clearly indicate that these novel benzimidazole-pyrazole hybrids can present a new class of potential anti ulcer agents and can serve as new anti-ulcer drugs after further investigation. Graphical abstract An overveiw of synthesis, in silico and in vivo antiulcer screening of benzimidazole pyrazole hybrids.</p>","PeriodicalId":9842,"journal":{"name":"Chemistry Central Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s13065-017-0314-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35507672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}