Mutagenicity, cytotoxic and antioxidant activities of Ricinus communis different parts.

Q1 Chemistry
Mazhar Abbas, Abid Ali, Muhammad Arshad, Asia Atta, Zahed Mehmood, Imtiaz Mahmood Tahir, Munawar Iqbal
{"title":"Mutagenicity, cytotoxic and antioxidant activities of Ricinus communis different parts.","authors":"Mazhar Abbas, Abid Ali, Muhammad Arshad, Asia Atta, Zahed Mehmood, Imtiaz Mahmood Tahir, Munawar Iqbal","doi":"10.1186/s13065-018-0370-0","DOIUrl":null,"url":null,"abstract":"<p><p>Ricinus communis (castor plant) is a potent medicinal plant, which is commonly used in the treatment of various ailments. The present study was conducted to appraise the cytotoxicity and mutagenicity of R. communis along with antioxidant and antimicrobial activities. Cytotoxicity was evaluated by hemolytic and brine shrimp assays, whereas Ames test (TA98 and TA100) was used for mutagenicity evaluation. Plant different parts were extracted in methanol by shaking, sonication and Soxhlet extraction methods. The R. communis methanolic extracts showed promising antioxidant activity evaluated as through total phenolic contents (TPC), total flavonoid content (TFC), DPPH free radical inhibition, reducing power and inhibition of linoleic acid oxidation. R. communis seeds, stem, leaves, fruit and root methanolic extracts showed mild to moderate cytotoxicity against red blood cells (RBCs) of human and bovine. Brine shrimp lethality also revealed the cytotoxic nature of extracts with LC<sub>50</sub> in the range of 0.22-3.70 (µg/mL) (shaking), 1.59-60.92 (µg/mL) (sonication) and 0.72-33.60 (µg/mL) (Soxhlet), whereas LC<sub>90</sub> values were in the range of 345.42-1695.81, 660.50-14,794.40 and 641.62-15,047.80 µg/mL for shaking, sonication and Soxhlet extraction methods, respectively. R. communis methanolic extracts revealed mild mutagenicity against TA98 (range 1975 ± 67 to 2628 ± 79 revertant colonies) and TA100 (range 2773 ± 92 to 3461 ± 147 revertant colonies) strains and these values were 3267 ± 278 and 4720 ± 346 revertant colonies in case of TA98 and TA100 positive controls, respectively. R. communis methanolic extracts prevented the H<sub>2</sub>O<sub>2</sub> and UV to Plasmid pBR<sup>322</sup> DNA oxidative damage. Results revealed that R. communis is a potential source of bioactive compounds and in future studies the bioactive compounds will be identified by advanced spectroscopic techniques.</p>","PeriodicalId":9842,"journal":{"name":"Chemistry Central Journal","volume":"12 1","pages":"3"},"PeriodicalIF":0.0000,"publicationDate":"2018-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5775190/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemistry Central Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s13065-018-0370-0","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Chemistry","Score":null,"Total":0}
引用次数: 0

Abstract

Ricinus communis (castor plant) is a potent medicinal plant, which is commonly used in the treatment of various ailments. The present study was conducted to appraise the cytotoxicity and mutagenicity of R. communis along with antioxidant and antimicrobial activities. Cytotoxicity was evaluated by hemolytic and brine shrimp assays, whereas Ames test (TA98 and TA100) was used for mutagenicity evaluation. Plant different parts were extracted in methanol by shaking, sonication and Soxhlet extraction methods. The R. communis methanolic extracts showed promising antioxidant activity evaluated as through total phenolic contents (TPC), total flavonoid content (TFC), DPPH free radical inhibition, reducing power and inhibition of linoleic acid oxidation. R. communis seeds, stem, leaves, fruit and root methanolic extracts showed mild to moderate cytotoxicity against red blood cells (RBCs) of human and bovine. Brine shrimp lethality also revealed the cytotoxic nature of extracts with LC50 in the range of 0.22-3.70 (µg/mL) (shaking), 1.59-60.92 (µg/mL) (sonication) and 0.72-33.60 (µg/mL) (Soxhlet), whereas LC90 values were in the range of 345.42-1695.81, 660.50-14,794.40 and 641.62-15,047.80 µg/mL for shaking, sonication and Soxhlet extraction methods, respectively. R. communis methanolic extracts revealed mild mutagenicity against TA98 (range 1975 ± 67 to 2628 ± 79 revertant colonies) and TA100 (range 2773 ± 92 to 3461 ± 147 revertant colonies) strains and these values were 3267 ± 278 and 4720 ± 346 revertant colonies in case of TA98 and TA100 positive controls, respectively. R. communis methanolic extracts prevented the H2O2 and UV to Plasmid pBR322 DNA oxidative damage. Results revealed that R. communis is a potential source of bioactive compounds and in future studies the bioactive compounds will be identified by advanced spectroscopic techniques.

Abstract Image

Abstract Image

蓖麻不同部位的突变性、细胞毒性和抗氧化活性。
蓖麻(蓖麻属植物)是一种有效的药用植物,常用于治疗各种疾病。本研究旨在评估蓖麻的细胞毒性和诱变性以及抗氧化和抗菌活性。细胞毒性通过溶血和盐水虾试验进行评估,致突变性评估则采用阿姆斯试验(TA98 和 TA100)。采用振荡、超声和索氏提取法在甲醇中提取植物的不同部分。通过总酚含量(TPC)、总黄酮含量(TFC)、DPPH 自由基抑制率、还原力和亚油酸氧化抑制率的评估,R. communis 的甲醇提取物显示出良好的抗氧化活性。R.communis种子、茎、叶、果实和根的甲醇提取物对人和牛的红细胞(RBC)具有轻度至中度的细胞毒性。盐水虾致死率也显示了提取物的细胞毒性,半数致死浓度范围为 0.22-3.70(微克/毫升)(振荡)、1.59-60.92(微克/毫升)(超声)和 0.72-33.60(微克/毫升)(振荡)。振荡、超声和索氏提取法的 LC90 值范围分别为 345.42-1695.81、660.50-14,794.40 和 641.62-15,047.80 µg/mL。R.communis甲醇提取物对TA98(变异菌落数范围为1975 ± 67至2628 ± 79)和TA100(变异菌落数范围为2773 ± 92至3461 ± 147)菌株具有轻微的诱变性,TA98和TA100阳性对照的变异菌落数分别为3267 ± 278和4720 ± 346。R.communis甲醇提取物可防止 H2O2 和紫外线对质粒 pBR322 DNA 的氧化损伤。研究结果表明,R. communis 是一种潜在的生物活性化合物来源,在今后的研究中,将通过先进的光谱技术对生物活性化合物进行鉴定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Chemistry Central Journal
Chemistry Central Journal 化学-化学综合
CiteScore
4.40
自引率
0.00%
发文量
0
审稿时长
3.5 months
期刊介绍: BMC Chemistry is an open access, peer reviewed journal that considers all articles in the broad field of chemistry, including research on fundamental concepts, new developments and the application of chemical sciences to broad range of research fields, industry, and other disciplines. It provides an inclusive platform for the dissemination and discussion of chemistry to aid the advancement of all areas of research. Sections: -Analytical Chemistry -Organic Chemistry -Environmental and Energy Chemistry -Agricultural and Food Chemistry -Inorganic Chemistry -Medicinal Chemistry -Physical Chemistry -Materials and Macromolecular Chemistry -Green and Sustainable Chemistry
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信