Cereal Research Communications最新文献

{"title":"Development of novel gluten-free bread based on fermented pseudo-cereals: effect on nutritional quality and textural properties","authors":"Zoulikha Tizemmour, Manel Mechmeche, Nesrine Messadi, Moktar Hamdi, Faten Kachouri","doi":"10.1007/s42976-024-00563-x","DOIUrl":"https://doi.org/10.1007/s42976-024-00563-x","url":null,"abstract":"<p>The purpose of this research was to determine the impact of an optimized gluten-free bakery flour (OGFBF) on technological quality, nutritional profile, essential elemental composition, total polyphenol content (TPC), antioxidant activity (DPPH), and the sensory evaluation of gluten-free bread. The findings show that the dough made from optimized gluten-free bakery flour that has been fermented with baker’s yeast results in good leavening dough (4.5 cm) as well as the production of various metabolites such as protein (7.41 g/100 g DM), total phenolic content (316.66 mg GAE/g DM), and antioxidant activity (2.6 mg/ml). The highest specific volume (2.77 cm³/g), the lowest hardness (433 g), and, of course, the taste of the bread are observed in the gluten-free OGFBF bread. During the sensory evaluation, the sample made from optimized gluten-free bakery flour (OGFBF bread) received higher scores compared with the reference sample (CGFF bread). The data obtained from this study could be helpful in the development of novel high-quality gluten-free bread for celiac patients.</p>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":"1 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142201501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre-storage treatments for suppressing of aflatoxins production in wheat grains","authors":"Mokhtar Mohamed Abdel-Kader, M. I. M. Ibrahim, Mohamed Saied Ali Khalil, Nehal Samy El-Mougy, Nadia Gamel El-Gamal","doi":"10.1007/s42976-024-00560-0","DOIUrl":"https://doi.org/10.1007/s42976-024-00560-0","url":null,"abstract":"<p>Wheat grains were collected from various locations in six governorates of Egypt and subjected to isolation trials. The isolated fungi were identified as <i>Aspergillus</i> spp. followed by <i>Alternaria</i> spp., <i>Fusarium</i> spp., <i>Rhizopus</i> spp. and other unidentified fungi, in that respective order. All isolated <i>A</i>. <i>flavus</i> strains (16 isolates) were screened for their ability to produce aflatoxins (AFs) on synthetic medium under long (365 nm wavelength) UV irradiation to determine their mycotoxin production capabilities. Data revealed that seven toxigenic isolates of <i>A. flavus</i> exhibited high fluorescent color. A preliminary test using TLC technique detected high production of aflatoxins by <i>A</i>. <i>flavus</i> isolates 2, 3, 4 and 6. The concentrations of AFs produced by positive <i>A. flavus</i> strains were determined using high-performance liquid chromatography (HPLC), to confirm high production of AFs by the 4 isolates. The <i>A</i>. <i>flavus</i> strain with the highest AFs production was selected for further studies. In laboratory conditions, the inhibitory effects of various organic acids, salts and essential oils were evaluated against the linear growth of <i>A. flavus</i>. Results indicated complete inhibition (100%) of fungal growth at 1.0% concentrations of malic acid, potassium sorbate, thyme and carnation oils. The production of aflatoxins in stored wheat grains treated with different concentrations of essential oils, organic acids and salts was studied over 45-day period. Untreated stored wheat grains showed high concentrations of AFG₁, AFG₂, AFB₁ and AFB₂ produced by <i>A. flavus</i> compared to grains treated with essential oils (thyme and carnation), malic acid and potassium sorbate. It was observed that all types of produced AFs gradually decreased with increasing concentrations of oil, acid or salt reaching their minimum levels at the highest concentration used (8%). The data demonstrated that the lowest aflatoxin production was recorded in grains treated with 8% potassium sorbate.</p>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":"69 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142201484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of Marrubium alysson and Torilis arvensis natural and nano extracts on priming of wheat seeds in response to drought","authors":"Dalia Youssef El-Berawey, Eman Mohammad Mustafa Eldebawy","doi":"10.1007/s42976-024-00539-x","DOIUrl":"https://doi.org/10.1007/s42976-024-00539-x","url":null,"abstract":"<p>Agriculture and climate change are inextricably linked in various aspects. Droughts have become more frequent as a result of climate change, having a significant impact on crop productivity. As a result, the current study investigated the effect of seed priming with natural plant extract and biosynthesized nano plant extract as an environmentally friendly tool for mitigating the drought effect on wheat as an economic crop. The study investigates the biosynthesis of Ag-nano particles from extracts of <i>Marrubium alysson</i> and <i>Torilis arvensis</i>. The UV–Vis spectrophotometer was used to characterize the biosynthesized AgNPs. Wheat grains were primed with <i>Marrubium alysson</i> and <i>Torilis arvensis</i>, along with their nanoextracts, and grown in different water regimes (100%, 60% and 40% field capacity), as well as hydropriming. Leaves were collected to determine the photosynthetic pigments, phenolics, flavonoids, CAT, GPX, H₂O₂, MDA, soluble sugars, and soluble proteins. In comparison with hydropriming seeds, the study discovered that natural and nano extracts significantly increased the CAT and GPX, as well as soluble proteins. Phenolics, flavonoids, soluble sugars, H₂O₂, and MDA content all decreased significantly, but pigment content remained unchanged. The study believed that priming wheat with natural and nano extracts, improved drought tolerance through the use of their metabolites, which included soluble sugars, phenolics, and flavonoids, accumulating in other metabolites like lignin, starch, and flavolignan to increase plant tolerance and reduce oxidative damage. Furthermore, nano extracts of <i>Torilis arvensis</i> and <i>Marrubium alysson</i> may be more effective than plant extracts since they separate from each other in PCA analysis.</p>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":"79 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141940519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of pulsed electric field on viability of Fusarium micromycetes","authors":"Jana Palicova, Jana Chrpova, Anna Tobolkova, Jaroslava Ovesna, Milena Stranska","doi":"10.1007/s42976-024-00561-z","DOIUrl":"https://doi.org/10.1007/s42976-024-00561-z","url":null,"abstract":"<p><i>Fusarium</i> species are one of the most studied fungal pathogens of cereals. They cause <i>Fusarium</i> head blight or stem base diseases that are risky not only in terms of yield losses but also due to mycotoxins production. Several strategies are used to prevent <i>Fusarium</i> colonisation of plants, including fungicide treatment. However, according to the EU Biodiversity Strategy for 2030, the use of fungicides needs to be reduced, and therefore, alternative seed treatments should be sought. One possible method is the use of a pulsed electric field (PEF) that could kill or remove the fungi from the grains surface. The sensitivity of fungi to PEF treatment was first investigated. Spore suspensions of four <i>Fusarium</i> species (<i>F. culmorum</i>, <i>F. graminearum</i>, <i>F. poae</i>, <i>F. sporotrichioides</i>) were treated in a continuous PEF system (OMNIPEF; VITAVE, Czech Republic), and spore viability was subsequently assessed on an artificial medium. Different species of the genus <i>Fusarium</i> showed statistically significant differences in their response to PEF. As the frequency increased from 450 to 900 Hz, the number of viable spores gradually decreased for all <i>Fusarium</i> species tested. <i>Fusarium poae</i> responded already at the lowest frequency of 450 Hz with a strong reduction in spore viability (only 9.4% of spores survived). At the same frequency, 15.3% of <i>F. graminearum</i> spores, 28.9% of <i>F. sporotrichioides</i> spores, and 53.4% of <i>F. culmorum</i> spores remained viable.</p>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":"71 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141887304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seed priming and subsequent inoculation of drought-tolerant plant growth-promoting rhizobacteria Bacillus tropicus KH90 improves rice (Oryza sativa L.) plant health under drought and non-drought conditions","authors":"Abu Barkat Md Gulzar, Pranab Behari Mazumder","doi":"10.1007/s42976-024-00559-7","DOIUrl":"https://doi.org/10.1007/s42976-024-00559-7","url":null,"abstract":"<p>Drought stress adversely affects productivity by altering plant health. Plant growth-promoting rhizobacteria (PGPR) can boost plant health and induce plant tolerance against various unfavorable conditions. This study aimed to screen drought-tolerant rhizobacteria with potent multi-PGP traits from previously isolated strains and evaluate the isolates’ efficacy on the enhancement of rice seed growth parameters under drought and non-drought conditions. Five isolates <i>(KK21, MK66, BH73, BH74, and KH90)</i> were found to be drought-tolerant with multi-PGP traits under both drought and non-drought conditions. The isolates also showed efficient oxidative stress mitigation features like proline accumulation, SA-production, and antioxidant (PPO, PO, and CAT) activities. The FTIR research revealed the presence of several functional groups in KH90-produced EPS under stressed and non-stressed circumstances. Using 16S rRNA gene sequencing, the drought-tolerant isolates were identified as <i>Bacillus cereus</i> KK21, <i>Lysinibacillus fusiformis</i> MK66, <i>Bacillus luti</i> BH73, <i>Bacillus aerius</i> BH74, and <i>Bacillus tropicus</i> KH90. Primed seeds performed better than unprimed seeds in terms of germination percentage, seedling height, and vigor index when grown in both drought and non-drought conditions. However, further plant inoculation with KH90 increased leaf relative water, photosynthetic pigments, and proline content and decreased electrolyte leakage and malondialdehyde contents in rice leaves under both drought and non-drought conditions. The results showed that drought-tolerant <i>Bacillus tropicus</i> KH90 with multi-PGP-traits is able to improve rice plant health in both normal and drought-stressed conditions by reducing the physiological damage caused by the stress. This gave credence to the idea that this strain may be used to manage drought stress.</p>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":"32 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141882565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microalgae–bacteria interaction: a catalyst to improve maize (Zea mays L.) growth and soil fertility","authors":"Wogene Solomon, Lamnganbi Mutum, Tibor Janda, Zoltan Molnar","doi":"10.1007/s42976-024-00558-8","DOIUrl":"https://doi.org/10.1007/s42976-024-00558-8","url":null,"abstract":"<p>Biofertilisers harbouring living organisms hold allure due to their prospective favourable influence on plant growth, coupled with a diminished environmental footprint and cost-effectiveness in contrast to conventional mineral fertilisers. The purpose of the present study was to evaluate the capacity of a specific microalga (MACC-612, <i>Nostoc linckia</i>) biomass and plant growth-promoting bacteria (PGPB) separately and together to improve crop growth and promote soil health. The research used a factorial design within a completely randomised block framework, featuring four replications for three consecutive years across different fields. The experiment utilised three levels of microalga (control, 0.3 g/L of <i>N. linckia</i>, MACC-612, and 1 g/L of <i>N. linckia</i>, MACC-612) and three levels of bacterial strains (control, <i>Azospirillum lipoferum</i> and <i>Pseudomonas fluorescens</i>). The result demonstrated that the use of <i>N. linckia</i> and PGPB separately or jointly as soil treatment resulted in a substantial improvement in chlorophyll, plant biomass, soil humus, and nitrogen, depending on the environmental conditions of the years. The combined use of <i>N. linckia</i> and PGPB results in an improvement in dry leaf weight by 35.6–107.3% at 50 days after sowing (DAS) and 29.6–49.8% at 65 DAS, compared to the control group. Furthermore, the studies show that the synergistic application of <i>N. linckia</i> at 0.3 g/L, in conjunction with <i>A. lipoferum</i>, significantly improved total nitrogen and (NO₃⁻ + NO₂⁻)-nitrogen, registering increases of 20.7–40% and 27.1–59.2%, respectively, during the study period. The most effective synergistic combination was identified through the application of 0.3 g/L of <i>N. linckia</i> along with <i>A. lipoferum</i>. Hence, application of biofertilisers through synergistic combinations of two or more microorganisms, such as microalgae and bacteria, holds promise in improving crop chlorophyll, growth, and soil nitrogen.</p>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":"165 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141870805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of two-rowed and six-rowed barley genotypes: impacts of water stress and nitrogen fertilizer on yield and stress responses","authors":"Siavash Bardehji, Mehrdad Mahlooji, Sara Zare, Mehmet Zeki Kocak, Bunyamin Yıldırım","doi":"10.1007/s42976-024-00555-x","DOIUrl":"https://doi.org/10.1007/s42976-024-00555-x","url":null,"abstract":"<p>Water stress is a critical abiotic stress factor with profound implications for agricultural productivity and global food security. This research explores the complex relationships between water stress and the application of nitrogen fertilizer in two-row and six-row barley genotypes with the aim of comprehending their performance and mechanisms for responding to stress. The study was performed over two years, employing a randomized complete block design with varying irrigation levels, nitrogen treatments, and barley genotypes. The results showed significant differences in various agro-morphological, physiological and biochemical traits between the two-row and six-row barley genotypes under different conditions. Water stress led to significant decreases in grain yield, shoot dry weight, leaf area index, and relative water content across all genotypes. It also caused an increase in electrolyte leakage, malondialdehyde content, and hydrogen peroxide levels, indicative of cellular membrane damage and oxidative stress. However, antioxidant enzyme activities such as glutathione peroxidase, ascorbate peroxidase, and catalase, along with DPPH radical scavenging activity, escalated as a defense response. Furthermore, nitrogen fertilizer application generally enhanced grain yield and shoot dry weight while decreasing oxidative stress indicators. However, under water stress conditions, nitrogen application exacerbated the negative effects of water stress, leading to reduced crop yield and stress resistance. As a result, this study emphasizes the critical role of genotype selection and proper use of nitrogen fertilizer application in optimizing the performance of two-row and six-row barley crops under different humidity conditions.</p>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":"81 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141870941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the potential of wild progenitors to enhance water deficit stress tolerance in maize","authors":"Gagandeep Kaur, Surinder Sandhu, Harjot Kaur, Nida Yousuf, Gagandeep Singh","doi":"10.1007/s42976-024-00554-y","DOIUrl":"https://doi.org/10.1007/s42976-024-00554-y","url":null,"abstract":"<p>The global challenge of water deficit stress (WDS) in maize (<i>Zea mays</i> L.) production, particularly in rainfed agriculture, necessitates a holistic understanding of root traits’ role in responding to this stress. While previous research has primarily focused on molecular mechanisms, acknowledging the significance of root architectural traits is essential. This study explores the development of the BC₂F₁ generation by crossing <i>Zea diploperennis</i> (donor) with maize inbred line LM13 (recurrent parent) to transfer water deficit tolerance attributes to maize. Efforts to develop backcross generations with <i>Z</i>. <i>nicaraguensis</i> were unsuccessful. The study investigates the response of wild maize relatives (<i>Z</i>. <i>diploperennis</i> and <i>Z</i>. <i>nicaraguensis</i>) and maize inbred line LM13 to WDS induced by osmolytes. Root trait modifications were analyzed using a root scanner, including parameters such as root projection area, length, tips, forks, segments, average root diameter, total root volume, number of metaxylem vessels, and xylem diameter. BC₂F₁ individuals, <i>Z</i>. <i>diploperennis</i>, and <i>Z</i>. <i>nicaraguensis</i> exhibited increased root projection area, total root length, and total root volume under WDS, while LM13 showed a decrease in most parameters. Scanning electron microscopy revealed increased metaxylem number in <i>Z</i>. <i>diploperennis</i> and BC₂F₁ under WDS, indicating drought tolerance. <i>Z</i>. <i>nicaraguensis</i> also displayed WDS tolerance. This study highlights the potential of wild maize relatives in conferring WDS tolerance and the feasibility of introgressing their root plasticity traits into cultivated maize for enhanced climatic resilience.</p>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":"34 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141777928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic analysis of quality protein maize (QPM): a review","authors":"Rajendra Vidadala, Vinay Kumar, Sanghamitra Rout, Poulami Sil, Vinay Teja, Mehdi Rahimi","doi":"10.1007/s42976-024-00552-0","DOIUrl":"https://doi.org/10.1007/s42976-024-00552-0","url":null,"abstract":"<p>Quality protein maize (QPM) is rich in lysine and tryptophan. QPM has notable variations in protein composition, critical amino acid profiles, and grain production potential. These variations significantly affect the nutritional value of our crops and the safety of the global food supply. For instance, QPM varieties can contain 60–100% more lysine and tryptophan than conventional maize. This increase in essential amino acids improves growth rates and health outcomes in populations relying on maize as a staple food. As we examine broad sense heritability estimates for these fundamental characteristics, it becomes clear that genetics significantly influences protein content. This means we can substantially increase the amount of protein in QPM through selective breeding. The essential amino acid composition, a defining feature of QPM, exhibits inheritable characteristics but with some fascinating complexities that call for more investigation. As a result of the complex trade-offs shown by the relationship between grain yield and these nutritional characteristics, we should consider comprehensive breeding approaches that consider several objectives. Genetic advance is an essential indicator of the efficiency of selection, and it gives us cause for hope. Within a relatively short period of generations, we may significantly improve the protein composition and the necessary amino acids. However, the intricate relationship between grain productivity and nutritional characteristics highlights the significance of comprehensive breeding programs that carefully maintain equilibrium between these objectives.</p><p>In conclusion, this study provides helpful direction for upcoming research and breeding projects by synthesizing the state of the art about the genetic foundation of QPM. Understanding the dynamics of variability, heredity, and genetic advance with grain production, essential amino acid content, and protein content is vital to maximizing QPM's potential to address global food security.</p>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":"68 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141778116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection and characterisation of DNA aptamer targeting immunogenic peptide sequence of high molecular weight glutenin (HMW-GS) of gluten","authors":"Jon Jyoti Kalita, Pragya Sharma, Utpal Bora","doi":"10.1007/s42976-024-00556-w","DOIUrl":"https://doi.org/10.1007/s42976-024-00556-w","url":null,"abstract":"<p>Immunogenic peptide epitopes that cause celiac disease are present in both the gliadin and glutenin fractions of the gluten protein found in cereals like wheat, rye and barley. Aptamers as a promising alternative to antibodies were developed only against the gliadin fraction and its constituent immunogenic peptide. However, for accurate assessment of the immunogenic peptides in food products through aptamer-based detection methods, there is a scope for developing aptamers against celiac disease epitopes in glutenin fractions. Here we report the development of aptamer against the immunogenic peptide sequence GQGQQGYYPTSPQQ of high molecular weight glutenin having celiac disease epitope. The aptamer was selected using magnetic bead-based SELEX (Mag-SELEX) method and characterised by ITC and circular dichroism. ITC experiment reveals that the dissociation constant (<i>K</i>_d) of selected aptamer Apt_J91P for primary binding site is 2.26 μM, and for secondary binding site, it is 4.385 mM in aptamer binding buffer. The binding mechanism of the aptamer with target was found to be enthalpy and entropy driven. Circular dichroism experiments show that aptamer forms stem and loop secondary structure. The limit of detection (LOD) of aptamer calculated by direct-ELAA method was found to be 16.0875 µM. We conclude that aptamer Apt_J91P can be successfully used for detecting celiac disease epitopes in glutenin and further improvement/modification would help in the development of sensitive aptasensors.</p>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":"1 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141777927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}