Cellular immunology最新文献_第9页

The effects of age, origin, and biological sex on rodent mast cell (BMMC and MC/9) and basophil (RBL-2H3) phenotype and function 年龄、来源和生物性别对鼠类肥大细胞(BMMC和MC/9)和嗜碱性粒细胞(RBL-2H3)表型和功能的影响

IF 4.3 4区医学

Cellular immunology Pub Date : 2023-09-01 DOI: 10.1016/j.cellimm.2023.104751

Ashley Wagner , Syed Benazir Alam , Marianna Kulka

{"title":"The effects of age, origin, and biological sex on rodent mast cell (BMMC and MC/9) and basophil (RBL-2H3) phenotype and function","authors":"Ashley Wagner , Syed Benazir Alam , Marianna Kulka","doi":"10.1016/j.cellimm.2023.104751","DOIUrl":"10.1016/j.cellimm.2023.104751","url":null,"abstract":"<div><p>Mast cells initiate allergic inflammatory immune responses and play a role in disease by releasing various inflammatory and immunomodulatory mediators. Several mast cell-lines and primary cultured cells have been used as mast cell models with inconsistent results among research groups. Bone marrow-derived mast cells (BMMC) cultured from mouse bone marrow progenitor cells are often used as a representative model of mucosal mast cell behaviour, however their reported phenotype is variable due to inconsistent culture protocols. RBL-2H3 is a rat basophilic histamine-releasing cell line that has some characteristics of both mast cells and basophils but is not a true representation of either cell type. The murine mast cell line MC/9 is an IL-3-dependent mucosal mast cell model but has limited mast cell characteristics. In this study, we have compared the response of BMMC (derived from C57BL/6 male or female mice), two sources of RBL-2H3 (purchased directly from ATCC and a lab curated culture), and MC/9 (ATCC) at several critical stages to some common stimuli (IgE/Ag, A23187) and analyzed mast cell morphology, expression level of common mast cell surface markers (CD117 and FcεRI), protease expression, and function (growth kinetics, viability, ROS production, degranulation, cytokine release and FcεRI signaling). The objective of this study was to provide insight into the effects of culture conditions, biological sex, and age of the cells on variability among reported phenotypes and, to determine optimal conditions for activation of these cells. Our data show that factors that are often overlooked such as source, age and biological sex of mast cells play an integral role in phenotypic outcomes and may account for the reported variability in their function.</p></div>","PeriodicalId":9795,"journal":{"name":"Cellular immunology","volume":"391 ","pages":"Article 104751"},"PeriodicalIF":4.3,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10623908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Crosstalk between peripheral immunity and central nervous system in Alzheimer’s disease 阿尔茨海默病中外周免疫与中枢神经系统的串扰

IF 4.3 4区医学

Cellular immunology Pub Date : 2023-09-01 DOI: 10.1016/j.cellimm.2023.104743

Hanchen Yang , Qi Qin , Meng Wang , Yunsi Yin , Ruiyang Li , Yi Tang

引用次数: 0

Piperine suppresses inflammatory fibroblast-like synoviocytes derived from rheumatoid arthritis patients Via NF-κB inhibition 胡椒碱通过抑制NF-κB抑制类风湿关节炎患者炎性成纤维细胞样滑膜细胞

IF 4.3 4区医学

Cellular immunology Pub Date : 2023-09-01 DOI: 10.1016/j.cellimm.2023.104752

Qoyama Noel Baito, Halmat M. Jaafar, Talar Ahmad Merza Mohammad

{"title":"Piperine suppresses inflammatory fibroblast-like synoviocytes derived from rheumatoid arthritis patients Via NF-κB inhibition","authors":"Qoyama Noel Baito, Halmat M. Jaafar, Talar Ahmad Merza Mohammad","doi":"10.1016/j.cellimm.2023.104752","DOIUrl":"10.1016/j.cellimm.2023.104752","url":null,"abstract":"<div><p>Rheumatoid Arthritis (RA) is a common autoimmune disease recognized by hyperplasia of synoviocytes and chronic joint inflammation. Activation of fibroblast-like synoviocytes (FLSs) is one of the main features of RA which can trigger inflammation leading to articular cartilage and joint destruction. Aberrant activation of NF-κB signaling cascade was found to be responsible for the high proliferation and defective apoptosis of FLSs and subsequent inflammation in RA. Piperine is a principal constituent of piper species frequently used as antitumor and anti-inflammatory natural compound. In this study we aimed to assess the anti-inflammatory effect of piperine on RA-FLS through NF-κB inhibition.</p><p>FLSs were isolated from 68 RA patients and 30 healthy controls and were exposed to piperine. The main assays were MTT assay, flow cytometric analysis, PI staining, reverse transcription-PCR (RT-PCR), and ELISA.</p><p>Results showed that piperine can induce the apoptosis and reduce the proliferation of RA-FLSs in vitro. Moreover, piperine directly reduced the phosphorylation of NF-kB and the expression of NF-κB target genes related to RA-FLSs proliferation (c-Myc and Cycline D1), apoptosis inhibition (Bcl2 and Bcl-xl) and inflammation (COX2, IL-1β, TNF-α,IL-6, CCL5 and CXCL10) while increasing the expression of apoptosis related ones (Bax) in vitro. Piperine also reduced the protein levels of cytokines and chemokines secreted by FLSs as a result of NF-κB inhibition.</p><p>In conclusion, our results provide evidence for the anti-inflammatory capacity of piperine through inhibition of NF-κB pathway in FLSs proposing this compound as a suitable alternative for chemical treatment of RA.</p></div>","PeriodicalId":9795,"journal":{"name":"Cellular immunology","volume":"391 ","pages":"Article 104752"},"PeriodicalIF":4.3,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10314434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phloretin suppresses intestinal inflammation and maintained epithelial tight junction integrity by modulating cytokines secretion in in vitro model of gut inflammation 在体外肠道炎症模型中，根皮素通过调节细胞因子分泌抑制肠道炎症，维持上皮紧密连接的完整性

IF 4.3 4区医学

Cellular immunology Pub Date : 2023-09-01 DOI: 10.1016/j.cellimm.2023.104754

Smita Kapoor , Yogendra S. Padwad

{"title":"Phloretin suppresses intestinal inflammation and maintained epithelial tight junction integrity by modulating cytokines secretion in in vitro model of gut inflammation","authors":"Smita Kapoor , Yogendra S. Padwad","doi":"10.1016/j.cellimm.2023.104754","DOIUrl":"10.1016/j.cellimm.2023.104754","url":null,"abstract":"<div><p>Ulcerative colitis is a type of inflammatory bowel disease which in long run can lead to colorectal cancer (CRC). Chronic inflammation can be a key factor for the occurrence of CRC thus mitigating an inflammation can be a preventive strategy for the occurrence of CRC. In this study we have explored the anti-inflammatory potential of phloretin, in <em>in vitro</em> gut inflammation model, developed by co-culture of Caco2 (intestinal epithelial) cells and RAW264.7 macrophages (immune cells). Phloretin is a dihydrochalcone present in apple, pear and strawberries. An anti-inflammatory effect of phloretin in reducing LPS induced inflammation and maintenance of transepithelial electric resistance (TEER) in Caco2 cells was examined. Paracellular permeability assay was performed using Lucifer yellow dye to evaluate the effect of phloretin in inhibiting gut leakiness caused by inflammatory mediators secreted by activated macrophages. Phloretin attenuated LPS induced nitric oxide levels, oxidative stress, depolarization of mitochondrial membrane potential in Caco2 cells as evidenced by reduction in reactive oxygen species (ROS), and enhancement of MMP, and decrease in inflammatory cytokines IL8, TNFα, IL1β and IL6. It exhibited anti-inflammatory activity by inhibiting the expression of NFκB, iNOS and Cox2. Phloretin maintained the epithelial integrity by regulating the expression of tight junction proteins ZO1, occludin, Claudin1 and JAM. Phloretin reduced LPS induced levels of Cox2 along with the reduction in Src expression which further regulated an expression of tight junction protein occludin. Phloretin in combination to sodium pyruvate exhibited potential anti-inflammatory activity via targeting NFkB signaling. Our findings paved a way to position phloretin as nutraceutical in preventing the occurrence of colitis and culmination of disease into colitis associated colorectal cancer.</p></div>","PeriodicalId":9795,"journal":{"name":"Cellular immunology","volume":"391 ","pages":"Article 104754"},"PeriodicalIF":4.3,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10313942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A mAb to SIRPα downregulates the priming of naive CD4 + T cell in Primary immune thrombocytopenia 对SIRPα的单抗下调原发性免疫性血小板减少症患者初始CD4 + T细胞的启动

IF 4.3 4区医学

Cellular immunology Pub Date : 2023-09-01 DOI: 10.1016/j.cellimm.2023.104757

Dongmei Xie, Zhihui Feng, Wen Yang, Yacan Wang, Renxia Li, Shiqi Zhang, Zeping Zhou

{"title":"A mAb to SIRPα downregulates the priming of naive CD4 + T cell in Primary immune thrombocytopenia","authors":"Dongmei Xie, Zhihui Feng, Wen Yang, Yacan Wang, Renxia Li, Shiqi Zhang, Zeping Zhou","doi":"10.1016/j.cellimm.2023.104757","DOIUrl":"10.1016/j.cellimm.2023.104757","url":null,"abstract":"<div><p>SIRPα is a transmembrane protein that binds the protein tyrosine phosphatases SHP-1 and SHP-2 through its cytoplasmic region and is abundantly expressed on monocytes, dendritic cells, and macrophages. Studies recently showed that SIRPα is essential for priming of CD4 + T cells by DCs and for development of Th17 cell-mediated autoimmune diseases. We have now further evaluated the importance of SIRPα and that of its ligand CD47 in primary immune thrombocytopenia (ITP). In this study, we show that there was a low expression state of SIRPα on the surface of monocytes. Treatment of cells culture from ITP patients with a mAb to SIRPα that blocks the binding of SIRPα to CD47 downregulated the ITP response. The abilities of monocytes from ITP patients to stimulate an allogenic MLR were reduced. The proliferation of, and production of IL-2, by CD4 + T cells from ITP patients were inhibited, the Treg cell numbers and the production of IL-10 pairs were upregulated, and the production of TGF-β not was inhibited, by a mAb to SIRPα. Moreover, a mAb to SIRPα, the expression of HLA-DR and CD86 were markedly inhibited and the expression of CD80 was slightly upregulated, on the surface of CD14 + monocytes from ITP patients as compared with healthy subjects. However, blockade of SIRPα increased the secretion of TLR-dependent cytokines TNF-α, IL-6 and IL-1β by PBMCs, which may be considered as a reserve in response to danger signals. These results suggest that SIRPα on monocytes is essential for the priming of naive T cells and the development of ITP. Therefore, SIRPα is a potential therapeutic target for ITP and other autoimmune diseases.</p></div>","PeriodicalId":9795,"journal":{"name":"Cellular immunology","volume":"391 ","pages":"Article 104757"},"PeriodicalIF":4.3,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10251235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential role for oral tolerance in gene therapy 口腔耐受在基因治疗中的潜在作用。

IF 4.3 4区医学

Cellular immunology Pub Date : 2023-09-01 DOI: 10.1016/j.cellimm.2023.104742

John S.S. Butterfield , Xin Li , Sreevani Arisa , Kwang-Chul Kwon , Henry Daniell , Roland W. Herzog

引用次数: 2

Tandem CAR-T cells targeting MUC1 and PSCA combined with anti-PD-1 antibody exhibit potent preclinical activity against non-small cell lung cancer 靶向MUC1和PSCA的串联CAR-T细胞结合抗pd -1抗体对非小细胞肺癌表现出强大的临床前活性

IF 4.3 4区医学

Cellular immunology Pub Date : 2023-09-01 DOI: 10.1016/j.cellimm.2023.104760

Aying Wang , Tangfeng Lv , Yong Song

引用次数: 0

HIF-1α regulates the expression of the non-conventional isoform of the cd5 gene in T cells under the hypoxic condition: A potential mechanism for CD5neg/low phenotype of infiltrating cells in solid tumors HIF-1α在低氧条件下调节T细胞中cd5基因非常规亚型的表达：实体瘤浸润细胞CD5neg/low表型的潜在机制

IF 4.3 4区医学

Cellular immunology Pub Date : 2023-09-01 DOI: 10.1016/j.cellimm.2023.104755

Smita Kumari, Srishti Sahu, Bharat Singh, Swarnima Gupta, Amit Kumar Kureel, Ankit Srivastava, Deeksha Rikhari, Sameer Srivastava, Ambak Kumar Rai

{"title":"HIF-1α regulates the expression of the non-conventional isoform of the cd5 gene in T cells under the hypoxic condition: A potential mechanism for CD5neg/low phenotype of infiltrating cells in solid tumors","authors":"Smita Kumari, Srishti Sahu, Bharat Singh, Swarnima Gupta, Amit Kumar Kureel, Ankit Srivastava, Deeksha Rikhari, Sameer Srivastava, Ambak Kumar Rai","doi":"10.1016/j.cellimm.2023.104755","DOIUrl":"https://doi.org/10.1016/j.cellimm.2023.104755","url":null,"abstract":"<div><p>CD5, a <em>T</em>-cell receptor (TCR) negative regulator, is reduced on the surface of CD8+ lymphocytes in the tumor microenvironment (TME). Reduced surface CD5 expression (sCD5) occurs due to the preferential transcription of HERV-E derived exon E1B, i.e., anon-conventional formofthe cd5gene instead of its conventional exon E1A. A tumor employs several mechanisms to evade anti-tumor response, and hypoxia is one such mechanism that prevails in the TME and modulates the infiltrated T lymphocytes. We identified hypoxia response elements (HREs) upstream of E1B. We showed binding of HIF-1α onto these HREs and increased E1B mRNA expression in hypoxic T cells. This results in decreased sCD5 expression and increased cytoplasmic accumulation in T cells. We also validated our study in a solid tumor, i.e., colorectal cancer (CRC) patient samples. This hypoxia-driven mechanism reduces the surface CD5 expression on infiltrated <em>T</em>-cells in solid tumors.</p></div>","PeriodicalId":9795,"journal":{"name":"Cellular immunology","volume":"391 ","pages":"Article 104755"},"PeriodicalIF":4.3,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49729664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autoimmune susceptible HLA class II motifs facilitate the presentation of modified neoepitopes to potentially autoreactive T cells 自身免疫易感的HLA II类基序促进修饰的新表位向潜在的自身反应性T细胞呈递

IF 4.3 4区医学

Cellular immunology Pub Date : 2023-08-01 DOI: 10.1016/j.cellimm.2023.104729

Antonis K. Moustakas , Hai Nguyen , Eddie A. James , George K. Papadopoulos

{"title":"Autoimmune susceptible HLA class II motifs facilitate the presentation of modified neoepitopes to potentially autoreactive T cells","authors":"Antonis K. Moustakas , Hai Nguyen , Eddie A. James , George K. Papadopoulos","doi":"10.1016/j.cellimm.2023.104729","DOIUrl":"10.1016/j.cellimm.2023.104729","url":null,"abstract":"<div><p>Rheumatoid arthritis (RA), multiple sclerosis (MS), type 1 diabetes (T1D), and celiac disease (CD), are strongly associated with susceptible HLA class II haplotypes. The peptide-binding pockets of these molecules are polymorphic, thus each HLA class II protein presents a distinct set of peptides to CD4<sup>+</sup> T cells. Peptide diversity is increased through post-translational modifications, generating non-templated sequences that enhance HLA binding and/or T cell recognition. The high-risk HLA-DR alleles that confer susceptibility to RA are notable for their ability to accommodate citrulline, promoting responses to citrullinated self-antigens. Likewise, HLA-DQ alleles associated with T1D and CD favor the binding of deamidated peptides. In this review, we discuss structural features that promote modified self-epitope presentation, provide evidence supporting the relevance of T cell recognition of such antigens in disease processes, and make a case that interrupting the pathways that generate such epitopes and reprogramming neoepitope-specific T cells are key strategies for effective therapeutic intervention.</p></div>","PeriodicalId":9795,"journal":{"name":"Cellular immunology","volume":"390 ","pages":"Article 104729"},"PeriodicalIF":4.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9796590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Chronic IFNα treatment induces leukopoiesis, increased plasma succinate and immune cell metabolic rewiring 慢性IFNα治疗诱导白细胞生成，血浆琥珀酸增加和免疫细胞代谢重接线

IF 4.3 4区医学

Cellular immunology Pub Date : 2023-08-01 DOI: 10.1016/j.cellimm.2023.104741

Anjali S. Yennemadi , Gráinne Jameson , Mary Glass , Carolina De Pasquale , Joseph Keane , Massimiliano Bianchi , Gina Leisching

{"title":"Chronic IFNα treatment induces leukopoiesis, increased plasma succinate and immune cell metabolic rewiring","authors":"Anjali S. Yennemadi , Gráinne Jameson , Mary Glass , Carolina De Pasquale , Joseph Keane , Massimiliano Bianchi , Gina Leisching","doi":"10.1016/j.cellimm.2023.104741","DOIUrl":"10.1016/j.cellimm.2023.104741","url":null,"abstract":"<div><p>Although clinically effective, the actions of IFNα, either produced endogenously or by therapeutic delivery, remain poorly understood. Emblematic of this research gap is the disparate array of notable side effects that occur in susceptible individuals, such as neuropsychiatric consequences, autoimmune phenomena, and infectious complications. We hypothesised that these complications are driven at least in part by dysregulated cellular metabolism. Male Wistar rats were treated with either 170,000 IU/kg human recombinant IFNα-2a or BSA/saline (0.9% NaCl) three times per week for three weeks. Bone marrow (BM) immune cells were isolated from the excised femurs for glycolytic rate and mitochondrial function assessment using Agilent Seahorse Technology. Frequencies of immune cell populations were assessed by flow cytometry to determine whether leukopoietic changes had occurred in both blood and BM. Plasma levels of lactate and succinate were also determined. BMDMs were metabolically assessed as above, as well as their metabolic response to an antigenic stimulus (iH37Rv). We observed that BM immune cells from IFN-treated rats exhibit a hypermetabolic state (increased basal OCR/GlycoPER) with decreased mitochondrial metabolic respiration and increased non-mitochondrial OCR. Flow cytometry results indicated an increase in immature granulocytes (RP1- SSChi CD45lo) only in the blood, together with increased succinate levels in the plasma. BMDMs from IFN-treated rats retained the hypermetabolic phenotype after differentiation and failed to induce a step-up in glycolysis and mitochondrial respiration after bacterial stimulation. This work provides the first evidence of the effects of IFNα treatment in inducing hypermetabolic immune features that are associated with markers of inflammation, leukopoiesis, and defective responses to bacterial stimulation.</p></div>","PeriodicalId":9795,"journal":{"name":"Cellular immunology","volume":"390 ","pages":"Article 104741"},"PeriodicalIF":4.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9800267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0