Calcified Tissue International最新文献_第2页

Impaired Remodeling of Tight Junctions Associated with Loss of Enamel Rod Decussation in Tgfbr2^G357W/+ Mice. Tgfbr2G357W/+小鼠紧密连接重构受损与牙釉质棒连接缺失相关

IF 3.2 3区医学

Calcified Tissue International Pub Date : 2026-04-30 DOI: 10.1007/s00223-026-01541-7

Olivier Duverger, Valentina Baena, Zulfeqhar A Syed, Janice S Lee

{"title":"Impaired Remodeling of Tight Junctions Associated with Loss of Enamel Rod Decussation in Tgfbr2G357W/+ Mice.","authors":"Olivier Duverger, Valentina Baena, Zulfeqhar A Syed, Janice S Lee","doi":"10.1007/s00223-026-01541-7","DOIUrl":"10.1007/s00223-026-01541-7","url":null,"abstract":"During enamel secretion, ameloblasts follow a complex coordinated movement that results in the enamel rods being arranged in a crisscross pattern that contributes significantly to its exceptional mechanical properties. We previously determined that mutations in the gene encoding TGF-β receptor 2 (TGFR-2) in humans (TGFBR2) and mice (Tgfbr2) result in the formation of fully mineralized enamel that exhibits impaired decussation of enamel rods. Using Tgfbr2G357W/+ mice, we demonstrated that this phenotype was due to disrupted coordinated movement of ameloblasts during enamel secretion. In this study, we used focused ion beam scanning electron microscopy (FIB-SEM) to look more closely at the area of the apical terminal web (ATW) where the cells are connected via tight junctions. We show that ameloblasts from wild-type littermates exhibit an abrupt shift in orientation at the ATW while Tgfbr2G357W/+ mice show no signs of such shift. Moreover, ZO-1 distribution at the ATW was abnormal in Tgfbr2G357W/+ mice with reduced accumulation at the ATW and presence of clusters of the protein at the basal side. These results suggest that abrupt orientation shift at the ATW via dynamic remodeling of tight junctions is essential for rows of ameloblasts to deposit enamel rods in opposite directions.","PeriodicalId":9601,"journal":{"name":"Calcified Tissue International","volume":"117 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13132921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147763305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amelogenin Phosphorylation Affects Protein-Protein Interactions In Vivo. 淀粉原蛋白磷酸化影响体内蛋白-蛋白相互作用。

IF 3.2 3区医学

Calcified Tissue International Pub Date : 2026-04-29 DOI: 10.1007/s00223-026-01530-w

Brent Vasquez, Ai Thu Bui, Henry C Margolis, Elia Beniash

引用次数: 0

Early Life Microplastic Exposure Impairs Mouse Incisor Enamel Formation. 早期接触微塑料损害小鼠门牙牙釉质形成。

IF 3.2 3区医学

Calcified Tissue International Pub Date : 2026-04-28 DOI: 10.1007/s00223-026-01535-5

Yiting Li, Jiahui Zheng, Sihan Yu, Qingquan Shi, Mian Wan, Liwei Zheng

引用次数: 0

Sex-Specific Associations of Radiographic Knee Osteoarthritis and Pain with Distal Tibia Bone Microarchitecture: the Study of Muscle, Mobility and Aging (SOMMA). 膝关节骨性关节炎和疼痛与胫骨远端骨微结构的性别特异性相关性：肌肉、活动和衰老的研究（SOMMA）。

IF 3.2 3区医学

Calcified Tissue International Pub Date : 2026-04-27 DOI: 10.1007/s00223-026-01531-9

Tong Yu, Andy Kin On Wong, Nina Z Heilmann, Kerri S Freeland, Bradley C Nindl, Kristen J Koltun, Andrew J Burghardt, Julie M Hughes, Peggy M Cawthon, Jane A Cauley, Elsa S Strotmeyer, Nancy E Lane

引用次数: 0

Serine-16 Phosphorylation, C-Terminal Truncation, and Ion-Specific Interactions Coordinate Amelogenin Nanoribbon Formation. 丝氨酸-16磷酸化，c端截断和离子特异性相互作用协调淀粉原纳米带的形成。

IF 3.2 3区医学

Calcified Tissue International Pub Date : 2026-04-27 DOI: 10.1007/s00223-026-01505-x

Emerson Tavares de Sousa, Johan Svensson Bonde, Kevin Lu, Yushi Bai, Stefan Habelitz

引用次数: 0

Micro-Raman Spectroscopy Reveals Compositional Alterations in Molar Incisor Hypomineralization and Enamel Adjacent to Demarcated Opacities. 微拉曼光谱揭示了臼齿低矿化和牙釉质附近有界浊斑的成分变化。

IF 3.2 3区医学

Calcified Tissue International Pub Date : 2026-04-27 DOI: 10.1007/s00223-026-01517-7

Flaureta Rexhaj, Furqan A Shah, Ted Lundgren

引用次数: 0

Mechanosensitive Piezo1 Channels in Enamel Cells. 釉质细胞中机械敏感的Piezo1通道。

IF 3.2 3区医学

Calcified Tissue International Pub Date : 2026-04-27 DOI: 10.1007/s00223-026-01534-6

Guilherme H Souza Bomfim, Anna Zou, Fabio A Echeverry, Ai Thu Bui, Edisa de Oliveira Sousa, Bruno Luis Graciliano Silva, Lukasz Witek, William A Coetzee, Rodrigo S Lacruz

{"title":"Mechanosensitive Piezo1 Channels in Enamel Cells.","authors":"Guilherme H Souza Bomfim, Anna Zou, Fabio A Echeverry, Ai Thu Bui, Edisa de Oliveira Sousa, Bruno Luis Graciliano Silva, Lukasz Witek, William A Coetzee, Rodrigo S Lacruz","doi":"10.1007/s00223-026-01534-6","DOIUrl":"https://doi.org/10.1007/s00223-026-01534-6","url":null,"abstract":"Ameloblasts are specialized epithelial cells that form enamel during the secretory and maturation stages, the latter involving an increase in Ca2+ transport to mineralize the enamel crystals. During enamel formation, ameloblasts travel several microns while secreting a matrix and are surrounded by several cell layers in the confined space of the enamel organ. Presumably, ameloblasts are subjected to mechanical stimuli e.g. pressure, stretch. Mechanosensitive (MS) or stretch-gated channels are expressed in the membranes of many cells including mineralizing cells. The opening of MS channels occurs in response to physical stimuli and results in the influx of ions. Piezo1 is a non-selective class of MS channel permeable to Ca2+ and hence it may contribute to Ca2+ homeostasis in ameloblasts. Here we show that secretory and maturation stage ameloblasts express similar protein levels of Piezo1. Cultured rat primary secretory and maturation stage ameloblasts showed stretch-activated currents by patch-clamp. Ameloblasts loaded with the cytosolic Ca2+ indicator Fura-2 were also stimulated with the Piezo1-selective activator Yoda1. We show that ameloblasts are sensitive to Piezo1 stimulation which evoked an increase in cytosolic Ca2+. This effect was inhibited by Piezo1 blockers. Mechanical analysis of the incisors of Piezo1 cKO mice showed no alterations in hardness or elastic modulus relative to littermate control mice. Our work provides the first evidence that Piezo1 channels are functional in both ameloblast stages and their activation leads to an elevation in cytosolic Ca2+, however, Piezo1 does not appear to be essential for enamel mineralization.","PeriodicalId":9601,"journal":{"name":"Calcified Tissue International","volume":"117 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147763298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clarifying the Distinction Between Pregnancy- and Lactation-Associated Osteoporosis and Transient Osteoporosis of the Hip. 澄清妊娠和哺乳期相关骨质疏松症和髋关节短暂性骨质疏松症的区别。

IF 3.2 3区医学

Calcified Tissue International Pub Date : 2026-04-27 DOI: 10.1007/s00223-026-01533-7

Şansın Tüzün

引用次数: 0

Region- and Compartment-Specific Elevation of Bone Mass in Mice Following Tsc1 Deletion in 8-kb Dmp1-Cre-Expressing Cells. 在8kb dmp1 - cre表达细胞中Tsc1缺失后小鼠骨量的区域和室特异性升高

IF 3.2 3区医学

Calcified Tissue International Pub Date : 2026-04-27 DOI: 10.1007/s00223-026-01532-8

Iya Ghassib, Anusha Inti, Nushaba Hossain, Thomas Kim, Danielle Moon, Lu Han, Rawan Mohsen, Honghao Zhang, Nicholas Auyeung, Yuji Mishina, Daniela Mendonça, Darnell Kaigler, Teresita Bellido, Fei Liu

引用次数: 0

Ano1 Antisense Therapy Improves Enamel Properties in Molars but not Amelogenesis in a Murine Model of Cystic Fibrosis. Ano1反义治疗可改善小鼠囊性纤维化模型臼齿的牙釉质特性，但不能改善成釉形成。

IF 3.2 3区医学

Calcified Tissue International Pub Date : 2026-04-27 DOI: 10.1007/s00223-026-01500-2

Dinne Nedjar, Arnaud Vanden Bossche, Christie Mitri, The Nghia Nguyen, Florian Hermans, Coralie Torrens, Nicolas Roubier, Elsa Vennat, David Montero, Lotfi Slimani, Hubert Marotte, Catherine Chaussain, Olivier Tabary, Françoise Tilotta

{"title":"Ano1 Antisense Therapy Improves Enamel Properties in Molars but not Amelogenesis in a Murine Model of Cystic Fibrosis.","authors":"Dinne Nedjar, Arnaud Vanden Bossche, Christie Mitri, The Nghia Nguyen, Florian Hermans, Coralie Torrens, Nicolas Roubier, Elsa Vennat, David Montero, Lotfi Slimani, Hubert Marotte, Catherine Chaussain, Olivier Tabary, Françoise Tilotta","doi":"10.1007/s00223-026-01500-2","DOIUrl":"https://doi.org/10.1007/s00223-026-01500-2","url":null,"abstract":"Cystic fibrosis (CF) is a life-shortening inherited disorder caused by mutations in the gene encoding the Cystic Fibrosis Transmembrane conductance Regulator (CFTR), a protein that controls the transport of chloride ions across cell membranes. Defects in CFTR cause thick mucus, leading to chronic infections and inflammation in multiple organs. Severe mutations, such as G542X, that completely abolish the function of CFTR present major therapeutic challenges. One potential approach is to stimulate an alternative chloride channel, ANO1, which is repressed in patients by the small regulatory molecule microRNA-9. We recently showed that an antisense oligonucleotide (ASO) designed to restore ANO1 activity (Ano1 ASO) improved respiratory and digestive functions in a CF mouse model carrying the pathogenic G542X variant. As patients with severe forms of CF often display enamel defects, we aimed to investigate the impact of Ano1 ASO on enamel of G542X CF mice. Combining multiproxy imaging approaches, our data showed that Ano1 ASO initiated at postnatal day 8 partially rescued the enamel properties in molars, which have mainly formed before the onset of treatment. However, it did not correct enamel maturation in the continuously growing incisor. In contrast to CFTR, there was no significant increase in ANO1 expression in maturation-stage ameloblasts compared to secretion ameloblasts. These findings suggest that while stimulating ANO1 cannot compensate for the absence of CFTR during enamel maturation, it enhances the mechanical properties of the erupted teeth, likely by improving salivary properties. This work identifies a potential avenue for mitigating dental complications in patients with severe CF mutations.","PeriodicalId":9601,"journal":{"name":"Calcified Tissue International","volume":"117 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147763073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0