Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie最新文献_第3页

The expected probabilities of identity for the circular stepping-stone model. 圆形踏脚石模型的期望同一性概率。

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1986-12-01 DOI: 10.1139/g86-147

C Strobeck

引用次数: 1

Symposium on Gene Regulation by Environmental Stress. Contributed paper session on stress-induced gene expression. London, Ont., Canada, 28 June 1985. 环境胁迫下的基因调控研讨会。贡献关于应激诱导基因表达的论文。伦敦。1985年6月28日，加拿大。

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1986-12-01

引用次数: 0

The synergistic effect of hyperthermia and ethanol on changing gene expression of mouse lymphocytes. 热疗与乙醇对小鼠淋巴细胞基因表达的协同作用。

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1986-12-01 DOI: 10.1139/g86-155

D I Rodenhiser, J H Jung, B G Atkinson

{"title":"The synergistic effect of hyperthermia and ethanol on changing gene expression of mouse lymphocytes.","authors":"D I Rodenhiser, J H Jung, B G Atkinson","doi":"10.1139/g86-155","DOIUrl":"https://doi.org/10.1139/g86-155","url":null,"abstract":"Cultured mouse lymphocytes respond to a brief incubation at an elevated temperature (41-43 degrees C) with the new and (or) enhanced synthesis of a select group of polypeptides (known as heat-shock proteins, HSPs) having relative molecular masses of 110, 100, 90, 70, and 65 kilodaltons (kDa). Expression of these HSPs is dependent on new RNA synthesis. Because the synthesis of any particular HSP is dependent on the temperature and the length of time cells remain at a particular elevated temperature, synthesis of each HSP is not necessarily coordinated with the synthesis of the other HSPs. Cultured mouse lymphocytes treated with arsenite or ethanol exhibit new and (or) enhanced synthesis of HSPs with molecular masses of 110, 90, 70, and 65 kDa but do not exhibit enhanced synthesis of the 100-kDa HSP. Short-term concurrent exposure of mouse lymphocytes to an elevated temperature and a level of ethanol, which individually do not induce detectable HSP synthesis, results in the pronounced synthesis of HSPs similar to those seen following exposure to higher levels of either stress applied separately. Thus, in this study we demonstrate that hyperthermia and ethanol stress can act synergistically to affect a dramatic change in the gene expression of mouse lymphocytes.","PeriodicalId":9589,"journal":{"name":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","volume":"28 6","pages":"1115-24"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/g86-155","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14947411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Differentiation of H genomes in the genus Critesion: evidence from synthetic hybrids involving Elymus and Critesion and one natural hybrid of C. violaceum and C. bogdanii crition属中H基因组的分化:来自Elymus和crition的合成杂种和C. violaceum和C. bogdanii的一个自然杂种的证据

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1986-12-01 DOI: 10.1139/G86-132

R. C. Wang, C. Hsiao

引用次数: 21

Immunochemical analysis of heat-shock protein synthesis in maize (Zea mays L.) 玉米热休克蛋白合成的免疫化学分析

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1986-12-01 DOI: 10.1139/G86-151

C. Baszczynski

引用次数: 5

Identification of mRNAs encoding low molecular mass heat-shock proteins in maize (Zea mays L.) 玉米(Zea mays L.)低分子量热休克蛋白编码mrna的鉴定

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1986-12-01 DOI: 10.1139/G86-154

C. A. Rees, N. C. Hogan, D. B. Walden, B. Atkinson

引用次数: 3

Murine plasmacytomas constitute a class of natural heat-shock variants in which the major inducible hsp-68 gene is not expressed. 小鼠浆细胞瘤构成一类自然热休克变异，其中主要的可诱导的热休克蛋白-68基因不表达。

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1986-12-01 DOI: 10.1139/g86-150

L Aujame

{"title":"Murine plasmacytomas constitute a class of natural heat-shock variants in which the major inducible hsp-68 gene is not expressed.","authors":"L Aujame","doi":"10.1139/g86-150","DOIUrl":"https://doi.org/10.1139/g86-150","url":null,"abstract":"Analysis of the heat-shock response in murine plasmacytomas reveals that, as demonstrated previously for the MPC-11 cell line, the genes coding for the 68-kilodalton heat-shock protein (hsp-68) are not expressed upon heat shock or sodium arsenite treatment. Noninduction is unique to the normally coordinated set of three hsp-68 genes since at least two other heat-shock protein genes (hsp-70 and hsp-89) are properly induced. No other lymphoid cell line was found to possess silent hsp-68 genes. Cell lines examined included a T lymphoma, a pre-B lymphocyte, and a non-B-non-T tumor cell line, as well as an Ig-nonproducing myeloma of undetermined differentiated status. Nonexpression is strain-independent as observed in BALB/c and C3H plasmacytomas. Based on S1 nuclease analysis using a cloned genomic hsp-68 probe, nonexpression is caused by the absence of hsp-68 mRNA following heat shock. A time-course experiment suggests that rapid degradation of mRNA does not occur, implying that the block is most likely at the transcriptional level. Southern blot analysis does not indicate any minor deletions around the region of transcription initiation, at least in the probed hsp-68 gene. These results suggest that the absence of hsp-68 gene expression may be a reflection of the differentiated and (or) transformed state of murine plasma cells, possibly through the absence or deregulation of a regulatory factor required for induction of heat-shock genes.","PeriodicalId":9589,"journal":{"name":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","volume":"28 6","pages":"1064-75"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/g86-150","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14163372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Role of heterochromatin during preferential 9q;22q translocation in chronic myelogenous leukemia. 异染色质在慢性粒细胞白血病中9q;22q优先易位中的作用。

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1986-12-01 DOI: 10.1139/g86-138

R S Verma, J Rodriguez, A Babu, S Chemitiganti, M Coleman, R T Silver, H Dosik

引用次数: 4

Heat shock induced changes in the gene expression of terminally differentiating avian red blood cells. 热休克诱导终分化禽红细胞基因表达的变化。

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1986-12-01 DOI: 10.1139/g86-149

B G Atkinson, R L Dean, T W Blaker

{"title":"Heat shock induced changes in the gene expression of terminally differentiating avian red blood cells.","authors":"B G Atkinson, R L Dean, T W Blaker","doi":"10.1139/g86-149","DOIUrl":"https://doi.org/10.1139/g86-149","url":null,"abstract":"Reticulocytes, purified from the blood of quail and chickens recovering from anaemia, respond to heat shock by the new and (or) enhanced synthesis of heat-shock protein (HSPs) with relative molecular masses of greater than 400,000, 90,000, 70,000, and 26,000 (quail) or 24,000 (chicken) and the depressed synthesis of many proteins normally produced at a control temperature. The synthesis of these HSPs is noncoordinate since the expression of each protein depends upon the particular temperature and duration of the time at that temperature. Separation of proteins from quail reticulocytes into Triton X-100 soluble and insoluble fractions demonstrates that the 70,000 and 26,000 Da HSPs are found in both fractions, whereas the greater than 400,000 and 90,000 Da HSPs are located only in the detergent-soluble fraction. Triton X-100 fractionation also reveals that there are three isoelectric variants of the 70,000 Da HSP and that they are constitutively synthesized and selectively partitioned between cellular compartments. Heat shock induced synthesis of the 90,000, 70,000, and 26,000 Da quail HSPs is prevented by actinomycin D, while enhanced synthesis of the greater than 400,000 Da HSP is unaffected by this inhibitor. These results demonstrate that nucleated, terminally differentiating avian red blood cells are capable of responding to heat stress by rapid changes in their highly restricted \"program\" of gene expression.","PeriodicalId":9589,"journal":{"name":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","volume":"28 6","pages":"1053-63"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/g86-149","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14947408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Heat-shock gene expression in animal embryonic systems. 动物胚胎系统热休克基因表达。

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1986-12-01 DOI: 10.1139/g86-153

J J Heikkila, L W Browder, L Gedamu, R W Nickells, G A Schultz

{"title":"Heat-shock gene expression in animal embryonic systems.","authors":"J J Heikkila, L W Browder, L Gedamu, R W Nickells, G A Schultz","doi":"10.1139/g86-153","DOIUrl":"https://doi.org/10.1139/g86-153","url":null,"abstract":"We have examined the expression of heat shock or stress genes in fish, echinoderm, amphibian, and mammalian embryonic systems. In a Chinook salmon embryo cell line, elevation of the incubation temperature or exposure to metal ions (e.g., cadmium and zinc) induced a set of heat-shock proteins HSPs. Transcriptional inhibitor, in vitro translation, and Northern hybridization studies suggest that fish HSP synthesis is regulated at the transcriptional level. The synthesis of HSPs during early development of Arbacia punctulata, Xenopus laevis, mouse, and rabbit is a stage-dependent phenomenon. In each of the developmental systems, HSP synthesis could not be induced until after cleavage stages. The ability of the embryo to undergo a heat-shock response (i.e., HSP synthesis) was correlated with the ability to detect HSP mRNA accumulation by either in vitro translation or Northern hybridization assays. Thus, the stage-dependent synthesis of HSPs appears to be controlled at the transcriptional level. Finally, in all of the organisms studied, the capacity to synthesize HSPs and accumulate HSP mRNA also coincides with acquisition of thermotolerance.","PeriodicalId":9589,"journal":{"name":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","volume":"28 6","pages":"1093-105"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/g86-153","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14947410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 71