Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie最新文献

Chromosome segregation from cell hybrids. II. Do differences in parental cell growth rates and phase times determine direction of loss? 杂种细胞的染色体分离。2亲代细胞生长速率和相时间的差异决定了损失的方向吗?

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1987-08-01 DOI: 10.1139/G87-115

J. Graves, J. M. Wrigley

{"title":"Chromosome segregation from cell hybrids. II. Do differences in parental cell growth rates and phase times determine direction of loss?","authors":"J. Graves, J. M. Wrigley","doi":"10.1139/G87-115","DOIUrl":"https://doi.org/10.1139/G87-115","url":null,"abstract":"The hypothesis that the direction of chromosome segregation in cell hybrids is determined by the interaction of parent cell cycles, or S-phase times, predicts that the segregant parent will always be the one with the longer cycle, or the longer S phase, and that late replicating chromosomes will be more frequently lost. We have tested this hypothesis by studying cell cycle parameters of mouse, Chinese hamster, and platypus parent cells and by observing chromosome loss and replication patterns in hybrids between them. Two types of hybrids have been studied: mouse-hamster hybrids showed gradual segregation, in one or other direction, of 10-60% chromosomes, while rodent-platypus hybrids (which could be selected under conditions optimal for either parent cell) showed rapid and extreme segregation of platypus chromosomes. We found no correlation between the direction of segregation and the relative lengths of parental cycle times, or phase times, nor between sequence of replication and frequency with which segregant chromosomes are lost. We therefore conclude that the direction and extent of segregation is not directly determined by the interaction of parental cycle or phase times.","PeriodicalId":9589,"journal":{"name":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","volume":"121 1","pages":"735-43"},"PeriodicalIF":0.0,"publicationDate":"1987-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90268742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Genetic analysis of blood pressure in the Milan hypertensive strain of rat (Rattus norvegicus). 褐家鼠米兰高血压品系血压遗传分析。

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1986-12-01 DOI: 10.1139/g86-134

G Schlager, B R Barber, G Bianchi

引用次数: 16

Expression and localization of hsp-23 in unstressed and heat-shocked Drosophila cultured cells 热休克和非应激果蝇培养细胞中热休克蛋白23的表达和定位

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1986-12-01 DOI: 10.1139/G86-152

J. Duband, F. Lettre, A. Arrigo, R. Tanguay

引用次数: 16

Sperm head morphology and nuclear chromatin structure evaluated by flow cytometry in a diallel cross in mice. 用流式细胞术评价双列杂交小鼠精子头形态和核染色质结构。

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1986-12-01 DOI: 10.1139/g86-133

B E Ballachey, W D Hohenboken, D P Evenson

{"title":"Sperm head morphology and nuclear chromatin structure evaluated by flow cytometry in a diallel cross in mice.","authors":"B E Ballachey, W D Hohenboken, D P Evenson","doi":"10.1139/g86-133","DOIUrl":"https://doi.org/10.1139/g86-133","url":null,"abstract":"Genetic factors affecting spermatogenesis, sperm morphology, and chromatin structure in mice were estimated using a diallel cross of the inbred lines C3H/HeJ, C57BL/6J, DBA/2J, and BALB/cByJ. Flow cytometry of acridine orange stained cells was used to evaluate proportions of testicular tetraploid, diploid, and haploid cells and nuclear chromatin structure of sperm, measured by resistance of chromatin to in situ acid denaturation, and quantified by the ratio of double- to single-stranded DNA (alpha t). Percent morphologically abnormal sperm was scored by light microscopy. Heterosis, line, maternal, and reciprocal effects, and general and specific combining abilities were estimated for body and testis weights, testicular cell proportions, sperm alpha t values, and percent abnormal sperm. Heterosis was important for testis weight, alpha t values, and percent abnormal sperm. Inbreds varied in body and testicular weights, alpha t values, and percent abnormal sperm. Significant maternal effects were noted for several traits but could be due to sex-linked (X or Y) factors, since maternal and sex-linked effects were confounded. Although a high positive correlation existed between alpha t values and percent abnormal sperm, the proportion of sperm with altered chromatin structure, measured by FCM, was generally much lower than proportion of morphologically abnormal cells.","PeriodicalId":9589,"journal":{"name":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","volume":"28 6","pages":"954-66"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/g86-133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14947412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temperature-stress response in maize: a comparison of several cultivars 玉米的温度胁迫反应:几个品种的比较

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1986-12-01 DOI: 10.1139/G86-156

R. Yacoob, W. Filion

引用次数: 26

A new method for producing homozygous duplications in maize 玉米纯合子复制的新方法

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1986-12-01 DOI: 10.1139/G86-144

W. R. Carlson, C. Curtis

引用次数: 8

Spontaneous mutability and colony morphology of Gibberella baccata 巴卡赤霉素的自发突变与菌落形态

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1986-12-01 DOI: 10.1139/G86-130

E. B. Lawrence, P. Nelson, T. Toussoun

引用次数: 2

Comportement meiotique de populations tetraploides du genre Dactylis 指爪属四倍体种群的减数分裂行为

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1986-12-01 DOI: 10.1139/G86-125

G. Guignard

引用次数: 2

Differential mitotic behaviour of genetically balanced and unbalanced microspores of an interchange heterozygote of Allium triquetrum 三尖葱交换杂合子遗传平衡与非平衡小孢子有丝分裂行为的差异

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1986-12-01 DOI: 10.1139/G86-129

G. K. Rickards

引用次数: 0

Use of Neurospora spore killer strains to obtain centromere linkage data without dissecting asci. 利用神经孢子虫孢子杀手菌株获得着丝粒连锁数据而无需解剖腹膜。

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie Pub Date : 1986-12-01 DOI: 10.1139/g86-135

D D Perkins, N B Raju, V C Pollard, J L Campbell, A M Richman

{"title":"Use of Neurospora spore killer strains to obtain centromere linkage data without dissecting asci.","authors":"D D Perkins, N B Raju, V C Pollard, J L Campbell, A M Richman","doi":"10.1139/g86-135","DOIUrl":"https://doi.org/10.1139/g86-135","url":null,"abstract":"Use of a centromere-linked Spore killer gene Sk reduces manyfold the labor involved in obtaining tetrad data that would otherwise require ordered dissection of intact linear eight-spored asci. Heterozygous crosses are made for Spore killer (SkK X SkS) and for markers to be tested. In such crosses only SkK ascospores survive. The four viable (SkK) and four aborted (SkS) ascospores of each ascus are ejected from the perithecium as a physically disordered group. The four surviving SkK ascospores of individual asci are germinated and scored. SkK segregates from SkS at the first meiotic division. If both marker alleles are represented in the surviving products, they must therefore have segregated from one another at the second division. Four-spore (Fsp) genes have been used to eliminate one postmeiotic nuclear division, so that only two ascospores per ascus need to be scored. The Spore killer method has been useful for mapping closely linked genes in centromere regions, for identifying genes that are far out on chromosome arms, for obtaining information on meiotic crossing-over, and for comparing linkages in different species.","PeriodicalId":9589,"journal":{"name":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","volume":"28 6","pages":"971-81"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/g86-135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14085910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12