Temperature-stress response in maize: a comparison of several cultivars

R. Yacoob, W. Filion
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引用次数: 26

Abstract

The protein synthetic response to a heat (28–41 °C) and a cold (28–4 °C) shock was studied in seedlings from 10 cultivars of maize with varying levels of cold tolerance. This response was compared by fluorography of one-dimensional polyacrylamide gels and immunoblot analysis. We utilized polyclonal antibodies against the 18 000 dalton (Da) heat-shock protein and the 73 000–89 000 Da heat-shock protein complex from Oh43 maize seedlings to ascertain antigenic similarity of these polypeptides. The heat-shock response varied in the numbers and relative molecular masses of the heat-shock proteins. Only three polypeptides appeared to be conserved across cultivars: a 93 000, 71 000, and 18 000 Da polypeptide. The cold-shock response varied from none to a dramatically altered pattern in a few cultivars. Thus, the heat- and cold-shock responses in these cultivars of corn differ in the types of polypeptides that are induced. All cultivars showed varying degrees of cross-reactivity when probed with the anti 18 000 D...
玉米的温度胁迫反应:几个品种的比较
研究了高温(28-41°C)和低温(28-4°C)对10个不同抗寒性玉米品种幼苗蛋白质合成的影响。通过一维聚丙烯酰胺凝胶的荧光成像和免疫印迹分析比较了这种反应。我们利用来自Oh43玉米幼苗的18000道尔顿(Da)热休克蛋白和73000 - 89000道尔顿热休克蛋白复合物的多克隆抗体来确定这些多肽的抗原相似性。热休克反应在热休克蛋白的数量和相对分子质量上有所不同。只有3个多肽在不同的品种中是保守的:93000、71000和18000 Da多肽。在一些品种中,冷休克反应从没有变化到急剧变化的模式。因此,这些玉米品种的热休克和冷休克反应在诱导多肽的类型上是不同的。所有品种均表现出不同程度的交叉反应。
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