Heat-shock gene expression in animal embryonic systems.

J J Heikkila, L W Browder, L Gedamu, R W Nickells, G A Schultz
{"title":"Heat-shock gene expression in animal embryonic systems.","authors":"J J Heikkila,&nbsp;L W Browder,&nbsp;L Gedamu,&nbsp;R W Nickells,&nbsp;G A Schultz","doi":"10.1139/g86-153","DOIUrl":null,"url":null,"abstract":"<p><p>We have examined the expression of heat shock or stress genes in fish, echinoderm, amphibian, and mammalian embryonic systems. In a Chinook salmon embryo cell line, elevation of the incubation temperature or exposure to metal ions (e.g., cadmium and zinc) induced a set of heat-shock proteins HSPs. Transcriptional inhibitor, in vitro translation, and Northern hybridization studies suggest that fish HSP synthesis is regulated at the transcriptional level. The synthesis of HSPs during early development of Arbacia punctulata, Xenopus laevis, mouse, and rabbit is a stage-dependent phenomenon. In each of the developmental systems, HSP synthesis could not be induced until after cleavage stages. The ability of the embryo to undergo a heat-shock response (i.e., HSP synthesis) was correlated with the ability to detect HSP mRNA accumulation by either in vitro translation or Northern hybridization assays. Thus, the stage-dependent synthesis of HSPs appears to be controlled at the transcriptional level. Finally, in all of the organisms studied, the capacity to synthesize HSPs and accumulate HSP mRNA also coincides with acquisition of thermotolerance.</p>","PeriodicalId":9589,"journal":{"name":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","volume":"28 6","pages":"1093-105"},"PeriodicalIF":0.0000,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/g86-153","citationCount":"71","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1139/g86-153","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 71

Abstract

We have examined the expression of heat shock or stress genes in fish, echinoderm, amphibian, and mammalian embryonic systems. In a Chinook salmon embryo cell line, elevation of the incubation temperature or exposure to metal ions (e.g., cadmium and zinc) induced a set of heat-shock proteins HSPs. Transcriptional inhibitor, in vitro translation, and Northern hybridization studies suggest that fish HSP synthesis is regulated at the transcriptional level. The synthesis of HSPs during early development of Arbacia punctulata, Xenopus laevis, mouse, and rabbit is a stage-dependent phenomenon. In each of the developmental systems, HSP synthesis could not be induced until after cleavage stages. The ability of the embryo to undergo a heat-shock response (i.e., HSP synthesis) was correlated with the ability to detect HSP mRNA accumulation by either in vitro translation or Northern hybridization assays. Thus, the stage-dependent synthesis of HSPs appears to be controlled at the transcriptional level. Finally, in all of the organisms studied, the capacity to synthesize HSPs and accumulate HSP mRNA also coincides with acquisition of thermotolerance.

动物胚胎系统热休克基因表达。
我们研究了热休克或应激基因在鱼类、棘皮动物、两栖动物和哺乳动物胚胎系统中的表达。在奇努克鲑鱼胚胎细胞系中,提高孵育温度或暴露于金属离子(如镉和锌)可诱导一组热休克蛋白HSPs。转录抑制剂、体外翻译和Northern杂交研究表明,鱼类热sp的合成受转录水平的调控。在斑草、非洲爪蟾、小鼠和家兔的发育早期,热休克蛋白的合成是一个阶段依赖性的现象。在每一个发育系统中,热休克蛋白的合成直到卵裂阶段之后才能被诱导。胚胎经受热休克反应(即热休克蛋白合成)的能力与通过体外翻译或Northern杂交检测热休克蛋白mRNA积累的能力相关。因此,热休克蛋白的阶段依赖性合成似乎在转录水平上受到控制。最后,在所研究的所有生物体中,合成HSP和积累HSP mRNA的能力也与耐热性的获得一致。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信