Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie最新文献

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Genetic complementation by somatic hybridization between pantothenate- and adenine-requiring auxotrophs of Datura innoxia P. Mill. 曼陀罗需要泛酸和腺嘌呤的营养缺陷体体细胞杂交的遗传互补。
P. Saxena, M. Mii, J. King
{"title":"Genetic complementation by somatic hybridization between pantothenate- and adenine-requiring auxotrophs of Datura innoxia P. Mill.","authors":"P. Saxena, M. Mii, J. King","doi":"10.1139/G86-142","DOIUrl":"https://doi.org/10.1139/G86-142","url":null,"abstract":"Auxotrophic complementation was achieved by protoplast fusion between two auxotrophic cell lines of Datura innoxia P. Mill. that required either pantothenate or adenine for their growth. Protoplast fusion was induced by polyethylene glycol enriched with calcium chloride at pH 6.8. Initial culture of fusion products in nonselective conditions increased the total number of somatic hybrids finally recovered under selective conditions. In addition to the prototrophy, the somatic hybrids regained the ability to turn green and some the ability to regenerate shoots on a cytokinin-supplemented medium. Both the parental cell lines grew as pale white callus without showing any sign of organogenesis, irrespective of the type and concentration of the cytokinin used. Approximately all the parental chromosomes (176 ± 8) were retained by the hybrid cell lines.Key words: protoplast, complementation, Datura.","PeriodicalId":9589,"journal":{"name":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","volume":"1 1","pages":"1022-1025"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76465149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Chromosomal structure of homozygous common wheat lines obtained from (wheat × Agropyron) × Aegilops speltoides derivatives. II. A screening of paracentric inversions 小麦× Agropyron × Aegilops speltoides纯合子系的染色体结构。2。准中心反转的筛选
Luis Ortiz, Á. González, M. Chueca, Y. Cauderon
{"title":"Chromosomal structure of homozygous common wheat lines obtained from (wheat × Agropyron) × Aegilops speltoides derivatives. II. A screening of paracentric inversions","authors":"Luis Ortiz, Á. González, M. Chueca, Y. Cauderon","doi":"10.1139/G86-126","DOIUrl":"https://doi.org/10.1139/G86-126","url":null,"abstract":"The presence of inversions is important in the evolution and cultivar differentiation of wheats, Even though we can't have conclusive cytological proof of the presence of inversions in wheat, we can use the indirect way of screening paracentric inversions through chromosome configuration at anaphase I and II. This work presents the comparison of 'Chinese Spring' structure (with respect to the presence of inverted segments) to (i) 52 homozygous wheat lines obtained in the progenies of the cross between a wheat × Agropyron intermedium substitution line and Aegilops speltoides homoeologous pairing promotor and (ii) three wheat cultivars ('Vilmorin 27', 'Ducat', and 'Tormes') that were used in developing the above mentioned lines. The three cultivars present inverted segments relative to 'Chinese Spring' structure. Some of these inverted segments are possibly maintained in some of the lines studied. Four of them show the primitive structure of 'Chinese Spring' even though none of the wheat parents had it. Fin...","PeriodicalId":9589,"journal":{"name":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","volume":"204 1","pages":"906-912"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86007791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Random environmental variation and inbreeding: effects on pure-strain and hybrid populations of flour beetles (Tribolium) 随机环境变异和近交:对粉甲虫纯种和杂交种的影响
D. Wool, O. Bergerson
{"title":"Random environmental variation and inbreeding: effects on pure-strain and hybrid populations of flour beetles (Tribolium)","authors":"D. Wool, O. Bergerson","doi":"10.1139/G86-124","DOIUrl":"https://doi.org/10.1139/G86-124","url":null,"abstract":"The effect of inbreeding and of random environmental variation on fitness characters was studied in small populations derived from 10 inbred laboratory strains of Tribolium castaneum (I) and in 18 ...","PeriodicalId":9589,"journal":{"name":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","volume":"1 1","pages":"889-898"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76086748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Genome analysis of Elymus alatavicus and E. batalinii (Poaceae: Triticeae) alatavicus和batalinii的基因组分析(禾本科:小麦科)
K. Jensen, D. R. Dewey, K. H. Asay
{"title":"Genome analysis of Elymus alatavicus and E. batalinii (Poaceae: Triticeae)","authors":"K. Jensen, D. R. Dewey, K. H. Asay","doi":"10.1139/G86-108","DOIUrl":"https://doi.org/10.1139/G86-108","url":null,"abstract":"Elymus alatavicus (Drob.) A. Love and E. batalinii (Krasn.) A. Love were studied to determine (i) meiotic behaviour, (ii) the mode of reproduction, (iii) the relationship between the two species, (iv) genomic constitutions, and (v) the most logical taxonomic classification of both species. A series of F1 hybrids between E. alatavicus, E. batalinii, and six \"analyzer\" species were developed. Chromosome pairing was studied at metaphase I to identify genomic similarities or differences. The results showed that E. alatavicus and E. batalinii are caespitose, self-fertile allohexaploids (2n = 42) with the same genomic formula SSYYXX. The F1 hybrids between E. alatavicus and E. batalinii had complete pairing (21 bivalents) at metaphase I in 7% of the cells and almost complete pairing in the remaining cells. High chromosome pairing and partial fertility (4 seeds/plant) in the F1 hybrids shows that the two species are closely related. Hybrids were obtained between E. alatavicus or E. batalinii and the following \"a...","PeriodicalId":9589,"journal":{"name":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","volume":"9 1","pages":"770-776"},"PeriodicalIF":0.0,"publicationDate":"1986-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75394997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Genetic modifications of dimorphic incompatibility in the Turnera ulmifolia L. complex (Turneraceae) 芜菁复合体二形不亲和性的遗传修饰
J. Shore, S. Barrett
{"title":"Genetic modifications of dimorphic incompatibility in the Turnera ulmifolia L. complex (Turneraceae)","authors":"J. Shore, S. Barrett","doi":"10.1139/G86-112","DOIUrl":"https://doi.org/10.1139/G86-112","url":null,"abstract":"Diploid and tetraploid populations of Turnera ulmifolia are distylous and exhibit a strong self-incompatibility system. Distyly is governed by a single locus with two alleles. Several self-compatible variants were, however, obtained and the nature and genetic control of self-compatibility was assessed using controlled crosses. The study documented the occurrence of self-compatible variants in four contrasting situations. These included the following. (i) Self-compatibility in a diploid short-styled variant. The gene(s) governing self-compatibility interact with the distyly locus and are expressed only in short-styled plants. When tetraploids carrying the genes were synthesized, self-incompatibility reappeared. (ii) Self-compatibility occurred in a cross between geographically separate diploid populations. Self-compatibility appeared sporadically in the F1. Crosses revealed that self-compatibility is likely under polygenic control. (iii) Low levels of self-compatibility occurred in a tetraploid population....","PeriodicalId":9589,"journal":{"name":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","volume":"15 1","pages":"796-807"},"PeriodicalIF":0.0,"publicationDate":"1986-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89089537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 22
The inheritance of genetic variation in rye (Secale cereale) affecting homoeologous chromosome pairing in hybrids with bread wheat (Triticum aestivum) 黑麦遗传变异对面包小麦(Triticum aestivum)杂种同源染色体配对的影响
P. Gupta, G. Fedak
{"title":"The inheritance of genetic variation in rye (Secale cereale) affecting homoeologous chromosome pairing in hybrids with bread wheat (Triticum aestivum)","authors":"P. Gupta, G. Fedak","doi":"10.1139/G86-117","DOIUrl":"https://doi.org/10.1139/G86-117","url":null,"abstract":"Chiasmata frequencies ranging from 0.07 to 10.40 per cell were recorded in 125 hybrid plants derived from wheat × F1 rye crosses. These included 89 plants belonging to 28 families from the Triticum aestivum 'Chinese Spring' × Secale cereale F1 ('Petkus' × 'Prolific') cross and 36 plants belonging to 11 families from the 'Chinese Spring' × F1 ('Prolific' × 'Puma') cross. The data were analyzed to study the inheritance of genetic variation in rye affecting homoeologous chromosome pairing. The results were particularly interesting in the former case where segregation for major genes was evident. First, in this cross, a bimodal distribution was observed and second, chiasmata frequencies ranging from 6.11 to 10.40 per cell were observed in three families but without any hybrid falling in either the range of 3.0 to 6.0 or in the range of 6.11 to 9.82, showing discontinuous distribution. It was concluded that the genetic system in 'Petkus' differs from that in 'Prolific', and that genes both with major effects a...","PeriodicalId":9589,"journal":{"name":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","volume":"55 1","pages":"844-851"},"PeriodicalIF":0.0,"publicationDate":"1986-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73454186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Gene interactions in Saccharomyces cerevisiae resulting in high-level resistance to the herbicide sulfometuron methyl 酿酒酵母菌基因相互作用导致对除草剂甲基磺脲的高水平抗性
G. H. Rank
{"title":"Gene interactions in Saccharomyces cerevisiae resulting in high-level resistance to the herbicide sulfometuron methyl","authors":"G. H. Rank","doi":"10.1139/G86-118","DOIUrl":"https://doi.org/10.1139/G86-118","url":null,"abstract":"The multiple drug resistant mutant pdrl-1 was used to select for independent isolates with resistance to high levels of the herbicide sulfometuron methyl. Tetrad analyses of isolates identified a p...","PeriodicalId":9589,"journal":{"name":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","volume":"194 1","pages":"852-853"},"PeriodicalIF":0.0,"publicationDate":"1986-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73117125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Chromosomal location of seed storage protein genes in the genome of Elytrigia elongata 长叶鞘翅种子贮藏蛋白基因的染色体定位
J. Dvorak, D. Kasarda, M. D. Dietler, E. J. Lew, O. Anderson, J. C. Litts, P. Shewry
{"title":"Chromosomal location of seed storage protein genes in the genome of Elytrigia elongata","authors":"J. Dvorak, D. Kasarda, M. D. Dietler, E. J. Lew, O. Anderson, J. C. Litts, P. Shewry","doi":"10.1139/G86-114","DOIUrl":"https://doi.org/10.1139/G86-114","url":null,"abstract":"Additions of complete and telocentric chromosomes of Elytrigia elongata (Host) Nevski in Triticum aestivum L. 'Chinese Spring' were employed to assign the genes coding for seed storage proteins to ...","PeriodicalId":9589,"journal":{"name":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","volume":"92 25 1","pages":"818-830"},"PeriodicalIF":0.0,"publicationDate":"1986-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81269754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 26
Heterochromatin composition and nucleolus organizer activity in four canid species. 四种犬科动物的异染色质组成和核仁组织器活性。
B Mayr, G Geber, H Auer, M Kalat, W Schleger
{"title":"Heterochromatin composition and nucleolus organizer activity in four canid species.","authors":"B Mayr,&nbsp;G Geber,&nbsp;H Auer,&nbsp;M Kalat,&nbsp;W Schleger","doi":"10.1139/g86-105","DOIUrl":"https://doi.org/10.1139/g86-105","url":null,"abstract":"<p><p>Sequential staining with a counterstain-contrasted fluorescent banding technique (chromomycin A3-distamycin A-DAPI) revealed the occurrence of distamycin A-4,6-diamidino-2-phenylindole (DA-DAPI) staining heterochromatin in the centromeric regions of chromosomes 33, 36, 37, and 38 in the wolf (Canis lupus pallipes) and of chromosomes 13, 16, and 23 in the blue fox (Alopex lagopus). The red fox (Vulpes vulpes) lacked such regions. Staining with DAPI--actinomycin D produced a QFH-type banding pattern with clearcut differences in the staining behaviour of DA-DAPI positive regions between these three canid species. Staining with the fluorochrome D 287/170 did not preferentially highlight any of the DA-DAPI positive regions in any of them. Counterstain-enhanced chromomycin A3 R-banding and studies of nucleolus organizer region location and activity confirmed a close relationship between the karyotype of the wolf and the domestic dog. Few heterochromatic marker bands were encountered in these two species, but heterochromatin polymorphism was evident in the blue fox.</p>","PeriodicalId":9589,"journal":{"name":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","volume":"28 5","pages":"744-53"},"PeriodicalIF":0.0,"publicationDate":"1986-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/g86-105","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14920773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Variability in the effect of alcohol on alcohol metabolizing enzymes may determine relative sensitivity to alcohols: a new hypothesis. 酒精对酒精代谢酶影响的变异性可能决定了对酒精的相对敏感性:一个新的假设。
S M Singh
{"title":"Variability in the effect of alcohol on alcohol metabolizing enzymes may determine relative sensitivity to alcohols: a new hypothesis.","authors":"S M Singh","doi":"10.1139/g86-111","DOIUrl":"https://doi.org/10.1139/g86-111","url":null,"abstract":"<p><p>Individual and racial differences in response to alcohol and with respect to alcoholism have strong genetic predispositions. Most studies on the actual genetic determinants have concentrated on the isozymes of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH), the two enzymes of the primary pathway of alcohol metabolism. Although few \"activity\" variants (associated with mutations in the structural genes) of the two enzymes are known to exist in susceptible groups, these observations do not offer an adequate explanation for the observed variability in response to alcohols in the population. Some recent studies have reported alterations in the specific activity of the two enzymes following exposure to alcohol for different lengths of time in man, rat, and mice. The induction-repression so observed is hypothesized to be regulated by one or more inducibility genetic elements (IGE) associated with the structural loci of the two enzymes. Variability in IGE will permit a genotype (individual) specific response in ADH and ALDH specific activity when challenged with a given level of alcohol. Considering the relative toxicity of acetaldehyde, the primary metabolite of this pathway, the resistant individuals would be expected to show ALDH induction. Conversely, the susceptible individuals should respond to alcohol by ALDH repression. The ability of an individual to show induction or repression following alcohol ingestion will depend on his or her IGE genotype(s) associated with specific enzyme loci. Also, the degree of polymorphism at these loci would be expected to be extensive and yet population and race specific. Once experimentally established, this approach could have important implications in screening, counselling, prevention, and in novel approaches to treatment.</p>","PeriodicalId":9589,"journal":{"name":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","volume":"28 5","pages":"789-95"},"PeriodicalIF":0.0,"publicationDate":"1986-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/g86-111","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14920774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
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