Access microbiology最新文献

{"title":"Bloodstream infections in cancer patients in central India: pathogens and trends of antimicrobial resistance over a 5-year period.","authors":"Sonali Choudhari, Ruchita Gawande, Jerestin Watchmaker, Pooja Bamnote, Pradeep Mishra, Pankaj Dwivedi","doi":"10.1099/acmi.0.000673.v5","DOIUrl":"https://doi.org/10.1099/acmi.0.000673.v5","url":null,"abstract":"<p><p><b>Introduction.</b> Bloodstream infection (BSI) is a common complication with a high fatality rate in cancer patients. There are notable variations in the epidemiology of BSI over time and among different countries. Infections due to multidrug-resistant organisms (MDROs) such as extended-spectrum beta-lactamases (ESBLs) and carbapenem-resistant <i>Enterobacteriaceae</i> (CRE) are increasing. This may lead to inadequate empirical antibiotic therapy, increasing the antimicrobial resistance (AMR) problem and unfavourable outcomes in these immunocompromised patients. There is paucity of data pertaining to AMR in such vulnerable patients from developing countries such as India. The aim of this study was to investigate the distribution of the bacterial pathogens causing BSI and the AMR trend in cancer patients in central India. <b>Methodology.</b> This single-centre retrospective observational study was conducted in a tertiary care cancer hospital. Patients with solid organ and haematological malignancies, both adults and paediatric, who had blood cultures sent to the microbiology laboratory from January 2018 to December 2022 were included. Blood cultures were processed using the BacT/ALERT 3D system (bioMérieux, France), and the identification of the bacteria and their antimicrobial susceptibility (AST) was performed using the Vitek 2 compact system (bioMérieux, France). Electronic medical records and microbiology lab records were used to retrieve the demographic and microbiological data. Microsoft Excel (RRID:SCR_016137) was used to enter and tabulate the data. Statistical analysis was performed using SPSS version 29 (RRID:SCR_002865). <b>Results.</b> A total of 687 isolates from 524 patients were studied. Gram-negative bacteria (64%) were the commonest cause of BSI in the studied patients, followed by Gram-positive cocci (25%) and fungal isolates (9%). Ten cases were polymicrobial. <i>Escherichia coli</i> (<i>n</i>=140) was the most common among the isolated pathogens, followed by <i>Klebsiella</i> species (<i>n</i>=103), <i>Pseudomonas</i> species (<i>n</i>=102), and coagulase-negative staphylococci (CONS) (<i>n</i>=92). Among the 140 isolates of <i>E. coli</i>, 66% were extended-spectrum β-lactamase (ESBL) producers and 26% were resistant to carbapenem. Among the 103 isolated <i>Klebsiella</i> species, 50% were carbapenem resistant and 36% were ESBL producers. Among enterobacterales, the CRE rate was 34%. Carbapenem resistance was seen in 25% of <i>Pseudomonas</i> species and 53% of <i>Acinetobacter</i> species isolates. <i>Klebsiella</i> species were the most resistant pathogens isolated. CONS comprised 56% of all Gram-positive isolates, followed by <i>Staphylococcus aureus</i> (36%), enterococci species (11%), and streptococci species (3%). Methicillin resistance was 60% in CONS and 64% in <i>S. aureus</i>. One vancomycin-resistant enterococcus was isolated. Non-<i>albicans Candida</i> was the most common fungal pathogen. The sensitivity ","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriological profile of community peritonitis operated in a Moroccan Hospital.","authors":"Samia Bazhar, Elmostafa Benaissa, Fatima Ziad, Leila Laamara, Yassine Ben Lahlou, Mariama Chadli, Mostafa Elouennass","doi":"10.1099/acmi.0.000816.v5","DOIUrl":"https://doi.org/10.1099/acmi.0.000816.v5","url":null,"abstract":"<p><p><b>Introduction.</b> Peritonitis is characterized by acute inflammation of the peritoneum, often resulting from digestive organ perforation or intra-abdominal septic focus. It may be of either infectious or non-infectious origin. The bacteria involved are those of the digestive flora (<i>Enterobacteriaceae</i> and anaerobes), while Gram-positive cocci and yeasts can be isolated in nosocomial infections. Our study aims to isolate and identify the germs involved in community-acquired peritonitis in order to assess their susceptibility to the antibiotics available in our country. <b>Methods.</b> This is a retrospective study of the bacteriological profile of community peritonitis in Rabat Morocco. A total of 150 adult patients with peritonitis were admitted and samples were collected intraoperatively for bacteriological examination between 1 July 2022 and 30 April 2023. <b>Results.</b> Among the 150 patients, 101 (67.8%) were males and 49 (32.2%) were females, with a sex ratio (M/F) of 2 : 1. The mean age of the patients was 40.5 years±20.12. The distribution of bacteria was dominated by <i>Escherichia coli</i> (44%). Overall, 70% of <i>E. coli</i> isolated exhibited resistance to ampicillin, whereas no resistance to ampicillin has been reported for <i>Enterococcus</i>. <b>Discussion.</b> In the present study, we were interested in the bacteriological profile of community peritonitis in order to adapt the antibiotic therapy to our bacterial ecology. Our findings indicate a concerning trend of increasing resistance among <i>E. coli</i> to the commonly used amoxicillin/clavulanic acid combination in our clinical setting. <b>Conclusion.</b> Consequently, there is a need to reassess the empiric antibiotic prescribed for the management of community-acquired peritonitis.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete sequences of pIJ101-based Streptomyces-Escherichia coli shuttle vectors.","authors":"Katelyn V Brown, S Eric Nybo","doi":"10.1099/acmi.0.000893.v3","DOIUrl":"https://doi.org/10.1099/acmi.0.000893.v3","url":null,"abstract":"<p><p>High-copy-number plasmids are indispensable tools for gene overexpression studies in prokaryotes to engineer pathways or probe phenotypes of interest. The development of genetic tools for the industrially relevant Actinobacteria is of special interest, given their utility in producing keratolytic enzymes and biologically active natural products. Within the Actinobacteria, <i>Streptomyces-Escherichia coli</i> shuttle vectors based on the SCP2* and pIJ101 incompatibility groups are widely employed for molecular cloning and gene expression studies. Here, the sequences of two commonly used pIJ101-based <i>Streptomyces-E. coli</i> shuttle vectors, pEM4 and pUWL201, were determined using next-generation sequencing. These plasmids drive the expression of heterologous genes using the constitutive <i>ermE*p</i> promoter. pEM4 was found to be 8.3 kbp long, containing a β-lactamase gene, thiostrepton resistance marker, the <i>lacZɑ</i> fragment, a ColE1 origin of replication and the <i>Streptomyces</i> pIJ101 origin of replication. pUWL201 was found to be 6.78 kbp long, containing a β-lactamase gene, thiostrepton resistance marker, the <i>lacZɑ</i> fragment, a ColE1 origin of replication and the <i>Streptomyces</i> pIJ101 origin of replication. Interestingly, the sequences for both pEM4 and pUWL201 exceed their previously reported size by 1.1 and 0.4 kbp, respectively. This report updates the literature with the corrected sequences for these shuttle vectors, ensuring their compatibility with modern synthetic biology cloning methodologies.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11498179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental sampling for the detection of capripox viruses and peste des petits ruminants virus in households and livestock markets in Plateau State, Nigeria.","authors":"Emma Brown, David Ehizibolo, Banenat B Dogonyaro, Yiltawe Wungak, Olumuyiwa Oyekan, Adeyinka Adedeji, Sandra Ijeoma, Rebecca Atai, Moses Oguche, Mark Samson, Fabrizio Rosso, Anna B Ludi, Georgina Limon, Andrew E Shaw, Claire Colenutt, Simon Gubbins","doi":"10.1099/acmi.0.000872.v3","DOIUrl":"10.1099/acmi.0.000872.v3","url":null,"abstract":"<p><p>Multiple transboundary animal diseases (TADs) circulate in Plateau State, Nigeria, where livestock keeping is common and contributes to both the physical and socio-economic well-being of a large proportion of the population. In this study, we explored the potential for environmental sampling to detect viruses causing TADs circulating in the region. Electrostatic dust cloths were used to swab areas of the environment likely to have contact with secretions and excretions from infected animals. Samples were collected monthly from five households, one transhumance site and one livestock market in two local government areas in Plateau State between March and October 2021. These were tested for the presence of peste des petits ruminants virus (PPRV) and capripox viruses using real-time PCR. Of the 458 samples collected, 2.4% (<i>n</i> = 11) were positive for PPRV RNA and 1.3 % (<i>n</i> = 6) were positive for capripox virus DNA. A capripox differentiation assay showed that these samples were positive for sheep pox virus (<i>n</i> = 2), goat pox virus (<i>n</i> = 2) and lumpy skin disease virus (<i>n</i> = 2). Our results demonstrate that environmental sampling could be used as part of TAD surveillance in the area. Environmental swabs require little technical knowledge to collect and can be used to detect multiple viruses from a single sample.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11488666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142485096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The complete genome sequence of five pre-2013 Escherichia coli sequence type (ST)1193 strains reveals insights into an emerging pathogen.","authors":"","doi":"10.1099/acmi.0.000894.v3","DOIUrl":"10.1099/acmi.0.000894.v3","url":null,"abstract":"<p><p>Fluoroquinolone-resistant <i>Escherichia coli</i> sequence type (ST)1193 is a profound, emerging lineage associated with systemic, urinary tract and neonatal infections. Humans, companion animals and the environment are reservoirs for ST1193, which has been disseminated globally. Following its detection in 2007, ST1193 has been identified repeatedly amongst fluoroquinolone-resistant clones in Australia. However, despite the growing importance of ST1193, only three complete genomes are published in the literature, none of which are from Australia. Here we expand on the available ST1193 resources with the complete genomes of five ST1193 strains sequenced using Oxford Nanopore Technologies and Illumina. Using <i>in silico</i> genotyping, we found that all strains were multi-drug resistant, including resistances to fluoroquinolones and cephalosporins. <i>In vitro</i> antibiotic susceptibility testing mostly correlated with individual genotypes. The exception was MS8320, which had additional <i>in vitro</i> resistance to piperacillin/tazobactam, ampicillin/sulbactam, cefazolin and doripenem (carbapenem). Further investigation identified seven additional copies of an IS26 transposable unit carrying a <i>bla</i> _TEM-1B beta-lactamase gene, suggesting this tandem amplification is associated with extended resistance phenotypes. Uropathogenicity factors, including three separate siderophore-encoding loci, were conserved in chromosomal and plasmid regions. Using all complete genomes, we further elucidated the recombination events surrounding the previously described K5/K1 capsular locus switch. Phenotypic confirmation of differing capsules in Australian ST1193 strains, coupled with genetic analysis revealing insertions downstream of the capsular locus, underscored the genetic distinctions between K5 and K1 capsule encoding strains. This study provides five new reference ST1193 genomes from Australia. These include the earliest complete K5-capsule ST1193 genomes on record (collected 2007), alongside our reference genome (MS10858), a clinical isolate obtained early during the ST1193 expansion and representative of the predominant K1-associated clade. These findings lay the foundations for further genomic and molecular analyses that may help understand the underlying reasons for the rapid global expansion of ST1193.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11488385/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142485098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial profile of wound site infections and evaluation of risk factors for sepsis among road traffic accident patients from Apex Trauma Centre, Northern India.","authors":"Aparna Singh, Sangram Singh Patel, Chinmoy Sahu, Amit Kumar Singh, Nidhi Tejan, Gerlin Varghese, Ashima Jamwal, Pooja Singh, Malay Ghar","doi":"10.1099/acmi.0.000836.v4","DOIUrl":"https://doi.org/10.1099/acmi.0.000836.v4","url":null,"abstract":"<p><p><b>Background.</b> Among the most significant yet often ignored health issues worldwide are trauma and accidental injuries. India accounts for 11% of global deaths in road accidents, the highest in the world, according to the World Bank report. There are limited data about the bacterial contamination of road traffic accident (RTA) wounds and their antibiotic susceptibility patterns. <b>Materials and Methods.</b> This prospective study was conducted in a tertiary care centre in northern India from January 2023 to January 2024. Wound deep swabs or aspirates were collected from RTA patients with traumatic injuries at different time intervals. Gram stain and culture were performed, and positive aerobic culture was subjected to antibiotic susceptibility testing. Organism identification was done using MALDI-TOF MS and routine biochemical tests. Blood samples were also collected to rule out bloodstream infections during follow-up if the patient became febrile or showed symptoms of systemic infection. Sepsis was defined in those patients who had two or more scores in the systemic inflammatory response syndrome criteria with a positive microbiological culture. Risk factors were evaluated for sepsis on the basis of the patient's vitals, injury characteristics, procalcitonin, Glasgow Coma Scale (GCS) score, need for mechanical ventilation and complete blood count, which were obtained from the patient's admission file. <b>Results.</b> A total of 189 wound samples were collected, of which 99 (52.38%) samples showed the growth of microorganisms. The aerobic isolates included 69 (69.69%) Gram-negative bacilli, of which the majority were <i>Klebsiella pneumoniae</i>, 28 (28.28%) Gram-positive cocci, of which the majority were <i>Staphylococcus aureus</i> and 2 (2.02%) anaerobic isolates. Among the Gram-negative isolates, none of the isolates were resistant to colistin. All <i>S. aureus</i> isolates were susceptible to vancomycin, teicoplanin and levonadifloxacin. Sepsis developed in 50 (26.45 %) patients. Significant risk factors evaluated for sepsis were a raised procalcitonin level, a low GCS score, a higher injury severity score, the need for mechanical ventilation and a raised quick sequential organ failure assessment score. <b>Conclusion.</b> It is essential to ascertain the profile of microorganisms isolated from RTA wounds in order to reduce antibiotic resistance and deliver efficient treatment.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11472858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142485095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spectrum of respiratory viruses identified from SARS-CoV-2-negative human respiratory tract specimens in Watansoppeng, Indonesia.","authors":"Irfan Idris, Isra Wahid, Ungke Antonjaya, Edison Johar, Fiqry Hasan Kleib, Ida Yus Sriyani, Aghnianditya Kresno Dewantari, Oderna Daming, Mustakim Duharing, Fatmawati Sappe, Hajar Hasan, Frilasita Aisyah Yudhaputri, Din Syafruddin, Khin Saw Aye Myint","doi":"10.1099/acmi.0.000840.v3","DOIUrl":"https://doi.org/10.1099/acmi.0.000840.v3","url":null,"abstract":"<p><p>Respiratory infections account for millions of hospital admissions worldwide. The aetiology of respiratory infections can be attributed to a diverse range of pathogens including viruses, bacteria and fungi. SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2)-negative specimens from Wattansoppeng city, South Sulawesi, were analysed to study the spectrum of respiratory viruses. Samples were screened for influenza virus, enterovirus, Paramyxoviridae, Nipah virus, Coronaviridae and Pneumoviridae. Of 210 specimens, 19 were positive for respiratory syncytial virus (RSV)-A, RSV-B, human parainfluenza virus type 1 (HPIV-1), HPIV-2, human rhinovirus (HRV)-A, HRV-B, HRV-C, human metapneumovirus (HMPV), influenza A virus (IAV) and coxsackievirus A6 (CV-A6). Influenza virus was of seasonal H3N2 subtype. The HMPVs were of genotypes B1 and A2a, while one RSV-A was of the ON-1 genotype. The viruses mostly affected children with unknown severity.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11469065/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142485097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbapenem-resistant Citrobacter amalonaticus and VRE bacteraemia in an immunocompetent patient after a urological Rezum procedure.","authors":"David Midlick, Jason Harhay, Nathan A Summers","doi":"10.1099/acmi.0.000852.v4","DOIUrl":"10.1099/acmi.0.000852.v4","url":null,"abstract":"<p><p>In this report, we discuss the case of a 62-year-old man who presented with gross haematuria, fever, and chills 1 day after undergoing a Rezum procedure and was found to have carbapenem-resistant <i>Citrobacter amalonaticus</i> and vancomycin-resistant <i>Enterococcus faecalis</i> bacteraemia. The patient was treated with daptomycin, eravacycline, and ceftalozane-tazobactam with positive results. We discuss our case and treatment of <i>C. amalonaticus</i> bacteraemia, a pathogen with limited existing literature on its incidence, presentation, and treatment.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11465632/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142402584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the Clostridioides difficile 630Δerm putative Pro-Pro endopeptidase CD1597.","authors":"Bart Claushuis, Arnoud H de Ru, Peter A van Veelen, Paul J Hensbergen, Jeroen Corver","doi":"10.1099/acmi.0.000855.v3","DOIUrl":"https://doi.org/10.1099/acmi.0.000855.v3","url":null,"abstract":"<p><p><i>Clostridioides difficile</i> is the leading cause of antibiotic-associated infections worldwide. Within the host, <i>C. difficile</i> can transition from a sessile to a motile state by secreting PPEP-1, which releases the cells from the intestinal epithelium by cleaving adhesion proteins. PPEP-1 belongs to the group of Pro-Pro endopeptidases (PPEPs), which are characterized by their unique ability to cleave proline-proline bonds. Interestingly, another putative member of this group, CD1597, is present in <i>C. difficile</i>. Although it possesses a domain similar to other PPEPs, CD1597 displays several distinct features that suggest a markedly different role for this protein. We investigated the proteolytic activity of CD1597 by testing various potential substrates. In addition, we investigated the effect of the absence of CD1597 by generating an insertional mutant of the <i>cd1597</i> gene. Using the <i>cd1597</i> mutant, we sought to identify phenotypic changes through a series of <i>in vitro</i> experiments and quantitative proteomic analyses. Furthermore, we aimed to study the localization of this protein using a fluorogenic fusion protein. Despite its similarities to PPEP-1, CD1597 did not show proteolytic activity. In addition, the absence of CD1597 caused an increase in various sporulation proteins during the stationary phase, yet we did not observe any alterations in the sporulation frequency of the <i>cd1597</i> mutant. Furthermore, a promoter activity assay indicated a very low expression level of <i>cd1597</i> in vegetative cells, which was independent of the culture medium and growth stage. The low expression was corroborated by our comprehensive proteomic analysis of the whole cell cultures, which failed to identify CD1597. However, an analysis of purified <i>C. difficile</i> spores identified CD1597 as part of the spore proteome. Hence, we predict that the protein is involved in sporulation, although we were unable to define a precise role for CD1597 in <i>C. difficile.</i></p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11460543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142396578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xanthomonas citri pv. eucalyptorum strain 4866-2_S43 (formerly X. axonopodis pv. eucalyptorum): the causal agent of bacterial leaf blight on eucalypts recovered in Argentina.","authors":"German Matias Traglia, Mousami Poudel, Samuel Miño, Blanca Isabel Canteros, G V Minsavage, Anuj Sharma, Erica M Goss, Jeffrey B Jones, Alberto Gochez","doi":"10.1099/acmi.0.000827.v3","DOIUrl":"https://doi.org/10.1099/acmi.0.000827.v3","url":null,"abstract":"<p><p>We report the draft genome assembly of strain 4866-2_S43 isolated from a eucalyptus lesion in Argentina and what until recently was caused by <i>Xanthomonas citri</i> pv. <i>eucalyptorum</i> (<i>Xce</i>). The genome size is 5 188 607 bp, with a G+C content of 64.66%. Comparative analysis reveals that the closest relative of strain 4866-2_S43 is <i>Xce</i> LPF 602, isolated in Brazil. Comparison of the whole genome sequences revealed an average nucleotide identity (ANI) of 99.96% between the two strains. ANIs were determined between the whole genome sequence of strain 4866-2_S43 and the genomes of all currently validated <i>Xanthomonas</i> spp. These results revealed that strain 4866-2_S43 shared >95% similarity with <i>X. citri</i> pv. <i>citri</i> and <i>X. citri</i> pv. <i>phaseoli</i>, and <95% with <i>X. euvesicatoria</i> pv. <i>alfalfae</i>, <i>X. euvesicatoria</i> pv. <i>perforans</i>, and <i>X. euvesicatoria</i> pathovars <i>euvesicatoria</i> and <i>eucalyptii</i>.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11475742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142485099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}