Access microbiologyPub Date : 2025-05-23eCollection Date: 2025-01-01DOI: 10.1099/acmi.0.001013.v3
Supathep Tansirichaiya, Wasawat Leartsiwawinyu, Nattharee Thanawan, Richard N Goodman, Chanwit Tribuddharat, Adam P Roberts
{"title":"Derivatization of pBACpAK entrapment vectors for enhanced mobile genetic element transposition detection in multidrug-resistant Escherichia coli.","authors":"Supathep Tansirichaiya, Wasawat Leartsiwawinyu, Nattharee Thanawan, Richard N Goodman, Chanwit Tribuddharat, Adam P Roberts","doi":"10.1099/acmi.0.001013.v3","DOIUrl":"10.1099/acmi.0.001013.v3","url":null,"abstract":"<p><p><b>Aim.</b> Antimicrobial resistance poses a critical global health threat, driven by the dissemination of resistance genes via mobile genetic elements (MGEs). This study aims to enhance the detection of MGE insertions in multidrug-resistant <i>Escherichia coli</i> by derivatizing the pBACpAK entrapment vector. <b>Methods and results.</b> Three derivatives were constructed with additional nucleotides upstream of the <i>cI</i> repressor gene, based on conserved regions identified from GenBank sequences containing known IS<i>26</i> and IS<i>1</i> insertions. Using colony PCR, intracellular transposition screening was performed on 194 tetracycline-resistant colonies from four <i>E. coli</i> ESI123 strains carrying different pBACpAK constructs. The derivatives showed increased MGE capture rates (10.7-73.1 %) compared to the WT vector (3.75%), identifying multiple MGEs, including the novel composite transposon Tn<i>7824</i>. Tn<i>7824</i> harbours the <i>bla</i> <sub>OXA-181</sub> carbapenem resistance gene and the <i>qnrS1</i> quinolone resistance gene, highlighting the clinical relevance of these findings. Long-read sequencing of transposants confirmed the accuracy of MGE identification and structural characterization, which also revealed chromosomal integration events of the pBACpAK derivatives mediated by flanking insertion sequences. <b>Conclusions.</b> The modifications introduced in the pBACpAK derivatives could increase the detection of transposition events by alleviating spatial constraints, allowing for more robust MGE detection.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12102499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144145347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2025-05-21eCollection Date: 2025-01-01DOI: 10.1099/acmi.0.000895.v3
Mohammed Labrigui, Hamza Layat, Manal Bouikhif, Fatima Ziad, Zimi Khalil, Zineb Jerroundi, Hafid Zahid, Zohra Ouzzif
{"title":"Fortuitous discovery of a microfilaria of the genus Loa loa during a routine blood smear at the Hematology Laboratory of the Mohamed V Military Instruction Hospital in Rabat: a case report.","authors":"Mohammed Labrigui, Hamza Layat, Manal Bouikhif, Fatima Ziad, Zimi Khalil, Zineb Jerroundi, Hafid Zahid, Zohra Ouzzif","doi":"10.1099/acmi.0.000895.v3","DOIUrl":"10.1099/acmi.0.000895.v3","url":null,"abstract":"<p><p>Loiasis is a parasitic infection transmitted by a vector, specifically through the bites of Chrysops genus tabanid flies. It is often associated with marked and persistent eosinophilia in affected individuals. We report the case of a 28-year-old Cameroonian male patient. His medical history includes an episode of malaria treated on an outpatient basis. As part of a diving internship in Morocco, the young serviceman underwent a medical fitness examination at the Medical Expertise Center for Aircrew Personnel of the Mohamed V Military Hospital, which included a biological assessment. This revealed a mildly elevated bilirubin level, lactate dehydrogenase activity at the upper limit of normal and eosinophilia at 1500 µl<sup>-1</sup>, without anaemia or thrombocytopaenia. A blood smear was prepared and stained with May-Grünwald Giemsa, revealing the presence of several small worms, with an appearance consistent with <i>Loa loa</i> microfilariae. This case of <i>L. loa</i>, identified in the haematology laboratory, is one of the rare diagnoses in Morocco. Therefore, biologists need to remain vigilant and carry out a thorough analysis of the blood count and blood smear.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12095865/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144129951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2025-05-21eCollection Date: 2025-01-01DOI: 10.1099/acmi.0.000879.v5
Saxon J Mwambene, Adelard B Mtenga, Augustino A Chengula, Abubakar S Hoza
{"title":"Detection and antibiotic resistance of Salmonella isolates from selected poultry farms in Dar es Salaam, Tanzania.","authors":"Saxon J Mwambene, Adelard B Mtenga, Augustino A Chengula, Abubakar S Hoza","doi":"10.1099/acmi.0.000879.v5","DOIUrl":"10.1099/acmi.0.000879.v5","url":null,"abstract":"<p><p><b>Introduction.</b> <i>Salmonella</i> is one of the most prevalent foodborne bacteria, posing a significant global health concern and responsible for ~155,000 deaths and 93.8 million human foodborne illnesses annually. The rampant use of antibiotic agents to combat salmonellosis in poultry has contributed to the emergence of resistance against commonly used antibiotics. <b>Methodology.</b> The cross-sectional study was conducted between January and June 2023 across three districts in the Dar es Salaam region, Tanzania. <i>Salmonella</i> isolates were detected and confirmed by using standard microbiological conventional methods and molecular methods such as PCR and genomic sequencing. PCR was used for detecting the presence of the <i>invA</i> gene, and partial DNA sequencing was performed to identify species and their close relatedness. A Kirby-Bauer disc diffusion method was employed to evaluate <i>Salmonella</i> sensitivity to seven different commonly used antibiotics, namely ampicillin, azithromycin, chloramphenicol, ciprofloxacin, gentamycin, trimethoprim/sulfamethoxazole and tetracycline. <i>Salmonella</i> strain with reference number ATCC 8739 was used as a control. <b>Results.</b> The overall <i>Salmonella</i> isolates from faecal droppings were 6.04% (<i>n</i>=796). Of the PCR-confirmed isolates, 64.3% (<i>n</i>=28) were resistant to more than two classes of antibiotics and hence considered multidrug resistant. The highest resistance was observed with ampicillin (92.9%, <i>n</i>=26), followed by tetracycline (71.43%, <i>n</i>=20), ciprofloxacin (42.9%, <i>n</i>=12), sulphonamide (42.85%, <i>n</i>=12), gentamicin (35.7%, <i>n</i>=10) and azithromycin (28.9%, <i>n</i>=8). All the isolates were susceptible to chloramphenicol (100%, <i>n</i>=28). Twenty-eight isolates were sent for sequencing, out of which 16 sequences (OR021717-OR021739) met the criteria for phylogenetic analysis. All 16 sequences had a per cent identity to EU348369 strains Senftenberg isolated from China, OL581594 <i>Salmonella</i> Newport isolated from China and EU348368 <i>Salmonella</i> Pullorum isolates from China. Other sequences diverged more distantly; these are <i>Salmonella</i> Abony with accession number CP007541 and <i>Salmonella</i> Kentucky with accession number OL581592. The tree also included an outgroup species, <i>Salmonella bongori</i>, which was downloaded from GenBank with accession numbers NC015761 and NC021870 <i>S. bongori</i>. <b>Conclusion.</b> The high level of antibiotic resistance found in this study could be due to the misuse of antibiotics in poultry management, and/or, probably, there were circulating resistant <i>Salmonella</i> strains in the environment. To reverse the trend observed, immediate interventions such as advocating for the prudent use of antibiotics in poultry production systems by strengthening extension services to poultry farmers and the use of a farmer field school model to improve poultry management through improved fa","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12095867/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144129947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2025-05-21eCollection Date: 2025-01-01DOI: 10.1099/acmi.0.000919.v3
Nabil Mohamed Zniber, Hamid Laatiris, Hamza Siyar, Abdelouahab Erraji, Ismail Labrouzi, Mohamed Jnah, Mehdi Talbi, Maryem Iken, Badreddine Lmimouni, Hafida Naoui
{"title":"Haematological abnormalities as diagnostic indicators of malaria in returning travellers: a retrospective study at Mohamed V Military Instruction Hospital.","authors":"Nabil Mohamed Zniber, Hamid Laatiris, Hamza Siyar, Abdelouahab Erraji, Ismail Labrouzi, Mohamed Jnah, Mehdi Talbi, Maryem Iken, Badreddine Lmimouni, Hafida Naoui","doi":"10.1099/acmi.0.000919.v3","DOIUrl":"10.1099/acmi.0.000919.v3","url":null,"abstract":"<p><p><b>Introduction.</b> Malaria remains a significant global health concern, particularly in travellers returning from endemic regions. Haematological abnormalities are often associated with malaria and can serve as diagnostic indicators, especially when clinical symptoms are nonspecific. <b>Objective.</b> This study aims to identify the most relevant haematological parameters for diagnosing malaria in travellers returning from endemic areas, who sought care at the Mohamed V Military Instruction Hospital in Rabat. <b>Methods.</b> We conducted a retrospective comparative study involving 829 patients who returned from malaria-endemic regions between January 2017 and December 2023. Data collected included demographic information, parasitological test results and comprehensive haematological profiles. Statistical analysis was performed to determine the sensitivity and specificity of various haematological parameters in diagnosing malaria. <b>Results.</b> Thrombocytopenia, lymphocytopenia and anaemia were the most significant haematological abnormalities associated with malaria. Thrombocytopenia, defined as a platelet count below 150×10³ µl<sup>-1</sup>, demonstrated a sensitivity of 75.91% and a specificity of 84.11%. Lymphocytopenia, with a threshold of less than 1.5×10³ µl<sup>-1</sup>, showed a sensitivity of 69.47% and a specificity of 78.39%. Anaemia, defined by haemoglobin levels below 13 g dl<sup>-1</sup> in men and 12 g dl<sup>-1</sup> in women, also significantly correlated with malaria diagnosis. <b>Discussion.</b> This study highlights the significance of haematological abnormalities as key diagnostic markers for imported malaria cases. By analysing retrospective data, we observed that these abnormalities, especially thrombocytopenia and anaemia, are common amongst returning travellers with confirmed malaria. These findings suggest that clinicians can use such markers as a valuable tool for early malaria diagnosis, potentially improving patient outcomes. Additionally, the study reinforces the need for heightened awareness amongst healthcare providers in non-endemic regions regarding the presentation of malaria in travellers. <b>Conclusion.</b> Haematological parameters such as thrombocytopenia, lymphocytopenia and anaemia are valuable diagnostic tools for malaria in returning travellers. These findings suggest that these parameters should be integrated into diagnostic protocols to improve the accuracy and timeliness of malaria diagnosis, particularly in clinical settings with limited access to advanced diagnostic tools.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12095866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144129952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2025-05-16eCollection Date: 2025-01-01DOI: 10.1099/acmi.0.000933.v3
Zakaria Malihy, Ikram El Abdallaoui, Tilila Abassor, Salah Sghir, Yassine Ben Lahlou, Rachid Abilkassem, Elmostafa Benaissa, Mariama Chadli
{"title":"First case of infective endocarditis due to NDM-type carbapenemase-producing Serratia marcescens in a preterm infant: a case report.","authors":"Zakaria Malihy, Ikram El Abdallaoui, Tilila Abassor, Salah Sghir, Yassine Ben Lahlou, Rachid Abilkassem, Elmostafa Benaissa, Mariama Chadli","doi":"10.1099/acmi.0.000933.v3","DOIUrl":"10.1099/acmi.0.000933.v3","url":null,"abstract":"<p><p><i>Serratia marcescens (S. marcescens)</i> is a Gram-negative rod-shaped bacterium belonging to the <i>Enterobacteriaceae</i> family, commonly found in various environments. This opportunistic pathogen can cause urinary tract infections, respiratory infections and septicaemia, but endocarditis is particularly rare and concerning due to its rapid and devastating progression. We report the first documented case worldwide of infective endocarditis (IE) caused by <i>S. marcescens</i> producing NDM-type carbapenemase, and the second reported case of <i>S. marcescens</i> endocarditis in a preterm infant. The patient was a preterm male infant born at 34 weeks of gestation, from a triplet pregnancy, admitted to the neonatal intensive care unit on day 2 of life for respiratory distress. The mother, aged 39, had undiagnosed gestational diabetes. Premature rupture of membranes had occurred 10 days before delivery, necessitating prophylactic treatment with amoxicillin. On day 4 of life, the newborn developed a fever with elevated C-reactive protein (CRP) levels and leucocytosis, leading to antibiotic therapy with colistin, imipenem and amikacin. Blood cultures revealed the presence of carbapenemase-producing <i>S. marcescens</i> sensitive to fluoroquinolones. A cardiac ultrasound showed a vegetation on the mitral valve, confirming the diagnosis of IE. Despite intensive treatment, the newborn died on day 16 of life due to septic shock. This rare case of endocarditis caused by <i>S. marcescens</i> highlights the severity of this infection in preterm infants. Treatment relies on appropriate antibiotic therapy. Prevention requires strict hygiene measures. Further research is needed to establish optimal therapeutic recommendations.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12084542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144096141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2025-05-16eCollection Date: 2025-01-01DOI: 10.1099/acmi.0.000990.v3
Rachelle Fernández-Vargas, Sara Jiménez-Alpízar, Valeria Leandro-Arce, Bradd Mendoza-Guido, Keilor Rojas-Jimenez
{"title":"Draft genome sequences of four potential new species of the genus Bradyrhizobium isolated from root nodules of native legumes in Costa Rican forests.","authors":"Rachelle Fernández-Vargas, Sara Jiménez-Alpízar, Valeria Leandro-Arce, Bradd Mendoza-Guido, Keilor Rojas-Jimenez","doi":"10.1099/acmi.0.000990.v3","DOIUrl":"10.1099/acmi.0.000990.v3","url":null,"abstract":"<p><p>Here, we report the draft genome sequences of four <i>Bradyrhizobium</i> spp. isolates obtained from root nodules of the native legumes <i>Pentaclethra macroloba</i>, <i>Chamaecrista nictitans</i>, <i>Erythrina fusca</i> and <i>Zygia engelsingii</i> in tropical forests of Costa Rica. Genomes ranged from 8.6 to 9.8 Mb with GC contents between 62.8% and 63.8%. Phylogenomic analysis, along with average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) comparisons, confirmed that these isolates represent potential new species. ANI values ranged from 88.3% to 90.3%, and dDDH values from 28.8% to 41.8%, compared with their closest <i>Bradyrhizobium</i> species. Functional annotation revealed some genes related to nitrogen fixation (<i>nifA</i>, <i>nifB</i>, <i>nifH</i>) and nodulation capacity (<i>nodB</i>, <i>nodC</i>, <i>nodJ</i>). These results provide insights into the diversity and symbiotic capabilities of <i>Bradyrhizobium</i> in tropical ecosystems.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12084545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144096137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2025-05-14eCollection Date: 2025-01-01DOI: 10.1099/acmi.0.000857.v4
Pradeep Kumar N, Ajithlal P M, Prasanta Saini, Aiswarya R S, Abidha Suresh, Philip Samuel, Balasubramaniam R, Jessu Mathew, Sonia T, Amju K P, Raju K H K, Veerapathiran A, Selvam A, Balaji T, Ashwani Kumar
{"title":"Is the Zika virus re-emerging as a distinct genetic lineage in India?","authors":"Pradeep Kumar N, Ajithlal P M, Prasanta Saini, Aiswarya R S, Abidha Suresh, Philip Samuel, Balasubramaniam R, Jessu Mathew, Sonia T, Amju K P, Raju K H K, Veerapathiran A, Selvam A, Balaji T, Ashwani Kumar","doi":"10.1099/acmi.0.000857.v4","DOIUrl":"https://doi.org/10.1099/acmi.0.000857.v4","url":null,"abstract":"<p><p>An outbreak of Zika fever occurred in Thiruvananthapuram City, Kerala, India, during 2021. At the request of the Kerala state health administration, we investigated the same, towards proposing requisite containment strategies for the disease outbreak. Epidemiological investigations indicated a clustering pattern of Zika fever cases with the presumed index case from a multi-speciality hospital in the city. Preliminary reports on the same had been already reported elsewhere during 2021. Further, entomological surveys carried out evinced the predominant mosquito species in the city, viz. <i>Aedes albopictus</i> (65.55%), <i>Aedes aegypti</i> (22.0%) and <i>Aedes vittatus</i> (12.0%) were naturally infected with Zika virus (ZIKV), the minimum infection rates being 17.9, 7.8 and 3.6, respectively. Also, trans-ovarian transmission was recorded in both <i>Ae. aegypti</i> and <i>Ae. albopictus</i>. This is the first report on the detection of ZIKV from <i>Ae. albopictus</i> in India. Analysis of phylogenetically informative genes of the ZIKV genome indicated the emergence of a distinct lineage of the Asian strain of virus, with five unique non-synonymous mutations, viz. 'A22T' and 'I160M' (pre-membrane) and 'D348N', 'T470A' and 'V473L' (envelope), that were involved in the outbreak. The altered gene expression pattern and evolutionary implications of these unique mutations remain to be investigated. Genetic analysis of the virus isolates from this and other investigations carried out on sporadic outbreaks of ZIKV in the country subsequently indicated that ZIKV is re-emerging as a distinct genetic lineage in India. These findings and other recent reports on ZIKV outbreaks warrant an urgent need for a systematic countrywide surveillance strategy, towards the prevention/preparedness/containment of a massive outbreak of this emerging neurovirulent arboviral disease.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2025-05-12eCollection Date: 2025-01-01DOI: 10.1099/acmi.0.000964.v3
Isabel Rancu, Benjamin Sobkowiak, Joshua L Warren, Nelly Ciobanu, Alexandru Codreanu, Valeriu Crudu, Caroline Colijn, Ted Cohen, Melanie H Chitwood
{"title":"Classification of unsequenced Mycobacterium tuberculosis strains in a high-burden setting using a pairwise logistic regression approach.","authors":"Isabel Rancu, Benjamin Sobkowiak, Joshua L Warren, Nelly Ciobanu, Alexandru Codreanu, Valeriu Crudu, Caroline Colijn, Ted Cohen, Melanie H Chitwood","doi":"10.1099/acmi.0.000964.v3","DOIUrl":"10.1099/acmi.0.000964.v3","url":null,"abstract":"<p><p>Over the past three decades, molecular epidemiological studies have provided new opportunities to investigate the transmission dynamics of <i>Mycobacterium tuberculosis</i>. In most studies, a sizable fraction of individuals with notified tuberculosis cannot be included, either because they do not have culture-positive disease (and thus do not have specimens available for molecular typing) or because resources for conducting sequencing are limited. A recent study introduced a regression-based approach for inferring the membership of unsequenced tuberculosis cases in transmission clusters based on host demographic and epidemiological data. This method was able to identify the most likely cluster to which an unsequenced strain belonged with an accuracy of 35%, although this was in a low-burden setting where a large fraction of cases occurred among foreign-born migrants. Here, we apply a similar model to <i>M. tuberculosis</i> whole-genome sequencing data from the Republic of Moldova, a setting of relatively high local transmission. Using a maximum cluster span of ~40 single nucleotide polymorphisms (SNPs) and a cluster size cutoff of <i>n</i>≥10, we could best predict the specific cluster to which each clustered case was most likely to be a member with an accuracy of 17.2 %. In sensitivity analyses, we found that a more restrictive (~20 SNPs threshold) or permissive (~80 SNPs) threshold did not improve performance. We found that increasing the minimum cluster size improved prediction accuracy. These findings highlight the challenges of transmission inference in high-burden settings like Moldova.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163731/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cardiac tamponade revealing tuberculous pericarditis: a case report.","authors":"Oumaima Skalante, Mariam Hachimi Idrissi, Soukaina Cherkaoui, Elmostafa Benaissa, Yassine Ben Lahlou, Mariama Chadli","doi":"10.1099/acmi.0.000983.v4","DOIUrl":"https://doi.org/10.1099/acmi.0.000983.v4","url":null,"abstract":"<p><p>Tuberculosis remains a major public health issue in Morocco. Pulmonary tuberculosis is the most common form, but various extrapulmonary forms exist. Tuberculous pericarditis is a rare form of extrapulmonary tuberculosis that can be complicated by cardiac tamponade, pericardial constriction or their combination, which can threaten the patient's life. Its clinical and radiological signs are nonspecific, and the clinical presentation can be misleading and incomplete, sometimes even with an initial tamponade. We report the case of a 68-year-old female patient admitted for intense retrosternal chest pain associated with acute dyspnoea, evolving in the context of unquantified weight loss and general deterioration. Additionally, she reported a history of fever and night sweats. Clinical examination revealed a conscious, febrile, hypotensive, tachycardic, polypneic patient with good oxygen saturation, signs of right heart failure and muffled heart sounds on auscultation. Chest X-ray revealed cardiomegaly, and the ECG showed diffuse low voltage. Given the presence of Beck's triad suggestive of cardiac tamponade, a transthoracic echocardiogram was performed, revealing a large pericardial effusion with a 'swinging heart'. A chest CT scan also confirmed the large pericardial effusion. The diagnosis of cardiac tamponade was made based on the clinical and radiological findings, and pericardial drainage was performed, after which the patient showed clinical improvement. PCR GenXpert MTB/RIF Ultra detected the presence of <i>Mycobacterium tuberculosis</i> in the pericardial fluid, with no resistance to rifampicin. Culture was positive for <i>M. tuberculosis</i>. The diagnosis of tuberculous pericarditis was, thus, confirmed, and the patient was started on quadruple antituberculosis therapy with good clinical progress.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048714/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144065545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2025-04-29eCollection Date: 2025-01-01DOI: 10.1099/acmi.0.000931.v4
T Slosser, M Wenick, E Markert, E Trembath-Reichert, L M Ward
{"title":"Novel hot spring Thermoproteota support vertical inheritance of ammonia oxidation and carbon fixation in Nitrososphaeria.","authors":"T Slosser, M Wenick, E Markert, E Trembath-Reichert, L M Ward","doi":"10.1099/acmi.0.000931.v4","DOIUrl":"https://doi.org/10.1099/acmi.0.000931.v4","url":null,"abstract":"<p><p>Aerobic ammonia oxidation is crucial to the nitrogen cycle and is only known to be performed by a small number of bacterial lineages [ammonia-oxidizing bacteria (AOB)] and a single lineage of archaea belonging to the <i>Nitrososphaeria</i> class of <i>Thermoproteota</i> [ammonia-oxidizing Archaea (AOA)]. Most cultivated AOA originate from marine or soil environments, but this may capture only a limited subset of the full diversity of this clade. Here, we describe several genomes of AOA from metagenomic sequencing of a hot spring microbial mat, representing several poorly characterized basal lineages that may be important for understanding the early evolution of archaeal ammonia oxidation. These genomes include a novel genus most closely related to <i>Nitrososphaera</i> as well as novel species belonging to the genera <i>Nitrosotenuis</i>, <i>Nitrososphaera</i> and <i>Nitrosotalea</i>. Furthermore, the distributions and phylogenetic relationships of key metabolic genes support a history of vertical inheritance of ammonia oxidation and carbon fixation from the last common ancestor of crown group AOA.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144045296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}