Turkish journal of biology = Turk biyoloji dergisi最新文献

{"title":"Development of an immunodiagnostic assay for the detection of <i>Sugarcane mosaic virus</i>.","authors":"Hafiz Muhammad Khalid, Najam Us Sahar Sadaf Zaidi, Naeem Rashid, Muhammad Tahir","doi":"10.55730/1300-0152.2714","DOIUrl":"https://doi.org/10.55730/1300-0152.2714","url":null,"abstract":"<p><strong>Background/aim: </strong><i>Sugarcane mosaic virus</i> (SCMV; genus <i>Potyvirus</i> and family <i>Potyviridae</i>), poses a significant threat to global sugarcane cultivars, including those in Pakistan. The aim of this study was to develop a rapid and effective diagnostic tool for detection of SCMV, enabling timely implementation of control measures to mitigate potential yield losses.</p><p><strong>Materials and methods: </strong>The study focused on the in silico analysis, physicochemical properties, immunogenicity, and subcellular localization of the SCMV coat protein (CP). The SCMV CP gene was synthesized, cloned, and expressed in <i>Escherichia coli</i>. The recombinant fusion CP (rFCP-SCMV) was purified and used to generate polyclonal antibodies (pABs) in mice. The immunogenicity of the antibodies was evaluated through indirect ELISA and RT-PCR.</p><p><strong>Results: </strong>Epitope prediction using tools like the OptimumAntigen design tool from GenScript, BepiPred, and IEDB identified key B-cell epitopes on the SCMV CP, enhancing the specificity of the antibodies. Structural modeling with SWISS-MODEL and PyMOL provided insights into the 3D structures of viral proteins and their epitopes, aiding in the design of high-affinity antibodies. Molecular docking studies simulated the interaction between antibodies and viral epitopes, enabling the selection of optimal antibody candidates. The synthesized recombinant fusion CP (rFCP-SCMV) was used to produce pAbs in mice. These antibodies exhibited high sensitivity, detecting as low as 100 pg of SCMV protein in indirect ELISA. They also effectively identified SCMV in infected sugarcane field samples, confirmed by RT-PCR. The antibodies maintained high specificity and sensitivity even at a 1:10,000 dilution, proving their efficacy in recognizing both the recombinant protein and virus particles in plant sap.</p><p><strong>Conclusion: </strong>The study reveals a rapid, effective immunodiagnostic technique for detecting SCMV in sugarcane cultivars, offering an accurate alternative to conventional virology methods, reducing contamination risk, and providing a valuable tool for mitigating yield losses.</p>","PeriodicalId":94363,"journal":{"name":"Turkish journal of biology = Turk biyoloji dergisi","volume":"48 6","pages":"390-400"},"PeriodicalIF":0.0,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11698196/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pterostilbene suppresses head and neck cancer cell proliferation via induction of apoptosis.","authors":"Talih Özdaş, Sibel Özdaş, İpek Canatar, Erdem Kaypak","doi":"10.55730/1300-0152.2708","DOIUrl":"https://doi.org/10.55730/1300-0152.2708","url":null,"abstract":"<p><strong>Background/aim: </strong>Head and neck cancer (HNC) is one of the most prevalent causes of death worldwide, and so discovering anticancer agents for its treatment is very important. Pterostilbene (PS) is a trans-stilbene reported to be beneficial in managing various cancers. The objective of the study was to evaluate the cytotoxic, antiproliferative, proapoptotic, and antimigrative effect of PS on HEp-2, SCC-90, SCC-9, FaDu, and Detroit-551 cell lines.</p><p><strong>Materials and methods: </strong>MTT and live/dead assays were employed to assess cell viability, while a cell migration test was performed to evaluate wound healing capacity. The mRNA, protein, and intracellular expression levels of <i>CASP-3</i>, <i>BAX</i>, and <i>BCL-2</i> genes were evaluated by real-time PCR, western blotting, and immunofluorescence staining. Annexin V-PI staining was conducted to assess the amounts of viable, apoptotic, and necrotic cells.</p><p><strong>Results: </strong>The results revealed that PS displayed cytotoxic, antiproliferative activity in a dose-dependent manner in HNC cells by upregulating <i>CASP-3</i> and <i>BCL-2</i> while downregulating <i>BCL-2</i> in the apoptotic pathway. The proapoptotic properties were confirmed by the annexin-V-IP results. Moreover, PS displayed a significant suppressive efficacy on the migration capacity of HNC cells.</p><p><strong>Conclusion: </strong>The present study provides proof that PS has the prospective to be improved as an attractive anticancer agent against HNC following advanced studies.</p>","PeriodicalId":94363,"journal":{"name":"Turkish journal of biology = Turk biyoloji dergisi","volume":"48 5","pages":"319-337"},"PeriodicalIF":0.0,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11518375/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optical imaging and gene transfection potential of linear polyethylenimine-coated Ag₂S near-infrared quantum dots.","authors":"Altay Savalan","doi":"10.55730/1300-0152.2709","DOIUrl":"https://doi.org/10.55730/1300-0152.2709","url":null,"abstract":"<p><strong>Background/aim: </strong>The application of biocompatible heavy metal-free and cationic Ag₂S NIR quantum dots (QDs), which have intense luminosity in the 700-900 nm medical range, as a nonviral gene delivery system paves the way to overcome autofluorescence and easily track the delivery of genes in real time.</p><p><strong>Materials and methods: </strong>The newly developed small and colloidally stable 2-mercaptopropionic acid (MPA)-capped Ag₂S aqueous quantum dots electrostatically complexed with linear polyethyleneimine (Ag₂S@2MPA/LPEI) were investigated for the first time both as a strong fluorescent probe and as a vector for nonviral gene delivery for the highest tracking of the system and delivery of genes into the nuclei of different cancer cells. The synthesized Ag₂S@2MPA/LPEI quantum dots demonstrated strong optical imaging properties and were used to deliver a green fluorescent protein (GFP) plasmid as a standard gene.</p><p><strong>Results: </strong>For Ag₂S@2MPA/LPEI-pDNA nanoparticles, an N/P ratio of 20 was the ideal transfection efficiency. Ag₂S@2MPA/LPEI was substantially more compatible with HEK 293T cells than the free 25-kDa linear polyethylenimine (LPEI). Next, the transfection efficiency evaluation of pGFP genes with synthesized Ag₂S@2MPA/LPEI and LPEI in different cancer cells demonstrated their high potential as a theranostic cancer gene delivery system.</p><p><strong>Conclusion: </strong>This is the first instance of gene transfection and optical imaging carried out in vitro using Ag₂S@2MPA/LPEI QDs. Overall, the newly synthesized highly biocompatible and trackable Ag₂S@2MPA/LPEI QDs can be an effective and biocompatible theranostic system for cancer gene therapy.</p>","PeriodicalId":94363,"journal":{"name":"Turkish journal of biology = Turk biyoloji dergisi","volume":"48 5","pages":"338-347"},"PeriodicalIF":0.0,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11518328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bud14 function is crucial for spindle pole body size maintenance.","authors":"Sevilay Münire Girgin, Ayşe Koca Çaydaşi","doi":"10.55730/1300-0152.2702","DOIUrl":"10.55730/1300-0152.2702","url":null,"abstract":"<p><strong>Background/aim: </strong>Spindle pole bodies (SPB), the functional equivalent of centrosomes in yeast, duplicate through generation of a new SPB next to the old one. However, SPBs are dynamic structures that can grow and exchange, and mechanisms that regulate SPB size remain largely unknown. This study aims to elucidate the role of Bud14 in SPB size maintenance in <i>Saccharomyces cerevisiae</i>.</p><p><strong>Materials and methods: </strong>We employed quantitative fluorescence microscopy to assess the relative and absolute amounts of SPB structural proteins at SPBs of wildtype cells and in cells lacking <i>BUD14</i> (<i>bud14Δ</i>). Quantifications were performed using asynchronous cell cultures, as well as cultures synchronously progressing through the cell cycle and upon different cell cycle arrests. We also utilized mutants that allow the separation of Bud14 functions.</p><p><strong>Results: </strong>Our results indicate that higher levels of SPB inner, outer, and central plaque proteins are present at the SPBs of <i>bud14Δ</i> cells compared to wildtype cells during anaphase, as well as during nocodazole-induced M-phase arrest. However, during α-factor mediated G1 arrest, inner and outer plaque proteins responded differently to the absence of <i>BUD14</i>. A Bud14 mutant that cannot interact with the Protein Phosphatase 1 (Glc7) phenocopied <i>bud14Δ</i> in terms of SPB-bound levels of the inner plaque protein Spc110, whereas disruption of Bud14-Kel1-Kel2 complex did not alter Spc110 levels at SPBs. In cells synchronously released from α-factor arrest, lack of Bud14-Glc7 caused increase of Spc110 at the SPBs at early stages of the cell cycle.</p><p><strong>Conclusion: </strong>We identified Bud14 as a critical protein for SPB size maintenance. The interaction of Bud14 with Glc7, but not with the Kelch proteins, is indispensable for restricting levels of Spc110 incorporated into the SPBs.</p>","PeriodicalId":94363,"journal":{"name":"Turkish journal of biology = Turk biyoloji dergisi","volume":"48 4","pages":"267-278"},"PeriodicalIF":0.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11407341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142305492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction of Griscelli Syndrome Type 2 causing mutations in the <i>RAB27A</i> gene with CRISPR/Cas9.","authors":"Özgür Doğuş Erol, Şimal Şenocak, Burcu Özçimen, Gülen Güney Esken, Hasan Basri Kiliç, Çetin Kocaefe, Niek P VAN Til, Fatima Aerts Kaya","doi":"10.55730/1300-0152.2705","DOIUrl":"https://doi.org/10.55730/1300-0152.2705","url":null,"abstract":"<p><strong>Background/aim: </strong>Griscelli Syndrome Type 2 (GS-2) is a rare, inherited immune deficiency caused by a mutation in the <i>RAB27A</i> gene. The current treatment consists of hematopoietic stem cell transplantation, but a lack of suitable donors warrants the development of alternative treatment strategies, including gene therapy. The development of mutation-specific clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 gene editing technology has opened the way for custom-designed gene correction of patient-derived stem cells. In this study, we aimed to custom design CRISPR/Cas9 constructs and test their efficiency on homology-directed repair (HDR) on the correction of exon 3 and exon 7 mutations in the <i>RAB27A</i> gene of GS-2 patient-derived mesenchymal stem cells (MSCs) and induced pluripotent stem cells.</p><p><strong>Materials and methods: </strong>We assessed <i>RAB27A</i> gene and protein expression using qRT-PCR, Western Blot, and immune fluorescence in GS-2 patient-derived MSCs and induced pluripotent stem cells (iPSCs). Guide RNAs (gRNAs) and donor DNAs were designed based on patient mutations in exon 3 and exon 7 using the CHOPCHOP online tool and transfected into GS-2 MSCs and iPSCs by electroporation. The cells were cultured for 2 days and then used for mutation analysis using DNA sequencing.</p><p><strong>Results: </strong>MSCs and iPSCs from the GS-2 patients lacked <i>RAB27A</i> gene and protein expression. After gRNA and donor DNAs were designed and optimized, we found HDR efficiency with gRNA3.3 (10% efficiency) and gRNA7.3 (27% efficiency) for MSCs but lower efficiency in iPSCs (<5%). However, transfection of both MSCs and iPSCs resulted in massive cell death, loss of colony formation, and spontaneous differentiation.</p><p><strong>Conclusion: </strong>The use of CRISPR/Cas9 to genetically correct MSCs and iPSCs from GS-2 patients with different mutations through HDR is feasible but requires optimization of the procedure to reduce cell death and improve stem cell function before clinical application.</p>","PeriodicalId":94363,"journal":{"name":"Turkish journal of biology = Turk biyoloji dergisi","volume":"48 5","pages":"290-298"},"PeriodicalIF":0.0,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11518329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expressions of the satellite repeat HSAT5 and transposable elements are implicated in disease progression and survival in glioma.","authors":"Sıla Naz Köse, Tutku Yaraş, Ahmet Bursali, Yavuz Oktay, Cihangir Yandim, Gökhan Karakülah","doi":"10.55730/1300-0152.2700","DOIUrl":"https://doi.org/10.55730/1300-0152.2700","url":null,"abstract":"<p><p>The glioma genome encompasses a complex array of dysregulatory events, presenting a formidable challenge in managing this devastating disease. Despite the widespread distribution of repeat and transposable elements across the human genome, their involvement in glioma's molecular pathology and patient survival remains largely unexplored. In this study, we aimed to characterize the links between the expressions of repeat/transposable elements with disease progression and survival in glioma patients. Hence, we analyzed the expression levels of satellite repeats and transposons along with genes in low-grade glioma (LGG) and high-grade glioma (HGG). Endogenous transposable elements LTR5 and HERV_a-int exhibited higher expression in HGG patients, along with immune response-related genes. Altogether, 16 transposable elements were associated with slower progression of disease in LGG patients. Conversely, 22 transposons and the HSAT5 satellite repeat were linked to a shorter event-free survival in HGG patients. Intriguingly, our weighted gene coexpression network analysis (WGCNA) disclosed that the HSAT5 satellite repeat resided in the same module network with genes implicated in chromosome segregation and nuclear division; potentially hinting at its contribution to disease pathogenesis. Collectively, we report for the first time that repeat and/or transposon expression could be related to disease progression and survival in glioma. The expressions of these elements seem to exert a protective effect during LGG-to-HGG progression, whereas they could have a detrimental impact once HGG is established. The results presented herein could serve as a foundation for further experimental work aimed at elucidating the molecular regulation of glioma genome.</p>","PeriodicalId":94363,"journal":{"name":"Turkish journal of biology = Turk biyoloji dergisi","volume":"48 4","pages":"242-256"},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11407350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142305494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the impact of diabetes on aging: insights from TERT and COL1A1 methylation.","authors":"Jessica Nathania Liamri, Farizky Martriano Humardani, Giovani Chandra, Lisa Thalia Mulyanata, Tjie Kok, Fenny Irawati, Hikmawan Wahyu Sulistomo, Christoph Reichetzeder, Sulistyo Emantoko Dwi Putra","doi":"10.55730/1300-0152.2701","DOIUrl":"https://doi.org/10.55730/1300-0152.2701","url":null,"abstract":"<p><strong>Background/aim: </strong>Aging, a multifaceted biological process, leads to diminished physical performance, especially in older adults with diabetes, where a mismatch between biological and chronological age is noticeable. Numerous studies have demonstrated that diabetes accelerates aging at the cellular and organ levels. Notable aging markers are telomerase reverse transcriptase (TERT), related to telomere length, and type 1 chain collagen (COL1A1), a key component of skin collagen. Additionally, age-related methylation increases, as revealed through methylation analysis, augmenting aspects of aging. However, the detailed interplay between aging and diabetes, particularly regarding methylation, remains underexplored and warrants further study to elucidate the biological links between the two.</p><p><strong>Materials and methods: </strong>In this study, we elucidate the modulatory influence of diabetes on the aging process, focusing specifically on the modifications in TERT in the kidney and COL1A1 in the skin using mice of Swiss Webster strain as the diabetes model. Specimens were categorized into three distinct chronological cohorts: chronologically young (16 weeks; n = 5), chronologically old (40 weeks; n = 5), and a periodically assessed group (16 weeks; n = 30), from which five mice were systematically sacrificed on a weekly basis.</p><p><strong>Results: </strong>Our findings reveal a marked impact of diabetes on the methylation statuses of TERT and COL1A1, characterized by an elevation in methylation levels within the periodic group (1st-6th week) and a simultaneous, progressive attenuation in the expression of TERT and COL1A1 genes.</p><p><strong>Conclusion: </strong>The observed alterations in the methylation levels of TERT and COL1A1 propound the hypothesis that diabetes potentially expedites the aging process, concomitantly impinging on the production of TERT and COL1A, ostensibly through the mechanism of promoter gene hypermethylation.</p>","PeriodicalId":94363,"journal":{"name":"Turkish journal of biology = Turk biyoloji dergisi","volume":"48 4","pages":"257-266"},"PeriodicalIF":0.0,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11407328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142305493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anatolian medicinal plants as potential antiviral agents: bridging traditional knowledge and modern science in the fight against COVID-19 and related viral infections.","authors":"Engin Tilkat, Israt Jahan, Ayşe Hoşer, Alevcan Kaplan, Oğuzhan Özdemir, Ahmet Onay","doi":"10.55730/1300-0152.2699","DOIUrl":"https://doi.org/10.55730/1300-0152.2699","url":null,"abstract":"<p><p>The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was the cause of the coronavirus 2019 (COVID-19), commonly known as the coronavirus pandemic. Since December 2020, COVID-19 vaccines have been extensively administered in numerous countries. In addition to new antiviral medications, the treatment regimen encompasses symptom management. Despite sustained research efforts, the outbreak remains uncontrolled, with affected patients still lacking proper treatment. This review is a valuable asset for researchers and practitioners aiming to delve into the yet unexplored potential of Anatolian flora in the fight against COVID-19 and other viral infections. Numerous medicinal plants in Anatolia, such as thyme, sage, cannabis, oregano, licorice root, and <i>Origanum</i> sp., contain bioactive compounds with proven antiviral properties that have been used in the region for centuries. The rich legacy of traditional Anatolian medicine (TAM), has significantly influenced modern medicine; thus, the profusion of medicinal plants native to Anatolia holds promise for antiviral drug development, making this review essential for researchers and practitioners.</p>","PeriodicalId":94363,"journal":{"name":"Turkish journal of biology = Turk biyoloji dergisi","volume":"48 4","pages":"218-241"},"PeriodicalIF":0.0,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11407354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142305491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organ-on-a-chip platforms for drug development, cellular toxicity assessment, and disease modeling.","authors":"Muhammad Maaz Khurram, Göktürk Cinel, Özlem Yeşil Çeliktaş, Erdal Bedir","doi":"10.55730/1300-0152.2711","DOIUrl":"https://doi.org/10.55730/1300-0152.2711","url":null,"abstract":"<p><p>Organs-on-chips (OoCs) or microphysiological platforms are biomimetic systems engineered to emulate organ structures on microfluidic devices for biomedical research. These microdevices can mimic biological environments that enable cell-cell interactions on a small scale by mimicking 3D in vivo microenvironments outside the body. Thus far, numerous single and multiple OoCs that mimic organs have been developed, and they have emerged as forerunners for drug efficacy and cytotoxicity testing. This review explores OoC platforms to highlight their versatility in studies of drug safety, efficacy, and toxicity. We also reflect on the potential of OoCs to effectively portray disease models for possible novel therapeutics, which is difficult to achieve with traditional 2D in vitro models, providing an essential basis for biologically relevant research.</p>","PeriodicalId":94363,"journal":{"name":"Turkish journal of biology = Turk biyoloji dergisi","volume":"48 6","pages":"348-363"},"PeriodicalIF":0.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11698198/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142934156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"O-demethyl galantamine alters protein expression in cerebellum of 5xFAD mice.","authors":"İrem Kiriş, Merve Karayel Başar, Büşra Gürel, Tomasz Mroczek, Ahmet Tarık Baykal","doi":"10.55730/1300-0152.2692","DOIUrl":"https://doi.org/10.55730/1300-0152.2692","url":null,"abstract":"<p><strong>Background/aim: </strong>Alzheimer's disease (AD), one of the most common health issues, is characterized by memory loss, severe behavioral disorders, and eventually death. Despite many studies, there are still no drugs that can treat AD or stop it from progressing. Previous in vitro tests showed that O-demethyl galantamine (ODG) might have therapeutic potential owing to its 10 times higher acetylcholinesterase inhibitory activity than galantamine (GAL).</p><p><strong>Materials and methods: </strong>We aimed to assess the effect of ODG at the molecular level in a 12-month-old 5xFAD Alzheimer's mouse model. To this end, following the administrations of ODG and GAL (used as a positive control), protein alterations were investigated in the cortex, hippocampus, and cerebellum regions of the brain. Surprisingly, GAL altered proteins prominently in the cortex, while ODG exclusively exerted its effect on the cerebellum.</p><p><strong>Results: </strong>GNB1, GNB2, NDUFS6, PAK2, and RhoA proteins were identified as the top 5 hub proteins in the cerebellum of ODG-treated mice. Reregulation of these proteins through Ras signaling and retrograde endocannabinoid signaling pathways, which were found to be enriched, might contribute to reversing AD-induced molecular changes.</p><p><strong>Conclusion: </strong>We suggest that, since it targets specifically the cerebellum, ODG may be further evaluated for combination therapies for AD.</p>","PeriodicalId":94363,"journal":{"name":"Turkish journal of biology = Turk biyoloji dergisi","volume":"48 3","pages":"163-173"},"PeriodicalIF":0.0,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11265889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141763921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}