Turkish journal of biology = Turk biyoloji dergisi最新文献

{"title":"Antimetastatic effect of nanodiamond-conjugated quercetin against colon cancer: an in vivo study.","authors":"Firli Rahmah Primula Dewi, Sephia Tiara Marviella, Sri Puji Astuti Wahyuningsih, A'liyatur Rosyidah, Vuanghao Lim, Lionel Lian Aun In, Amy Yi Hsan Saik, Bimaji Ariyogo, Mee Lee Looi","doi":"10.55730/1300-0152.2704","DOIUrl":"https://doi.org/10.55730/1300-0152.2704","url":null,"abstract":"<p><strong>Background/aim: </strong>Quercetin (Q) is a compound that can inhibit the growth of cancer cells in the colon; however, to do so, a high dose is needed, requiring a drug delivery system to target cancer endothelial cells directly. This study investigates the potency of nanodiamond-conjugated quercetin (NDQ) as an anticancer drug against colon cancer in <i>Rattus norvegicus</i> induced by N-methyl N-Nitrosourea (MNU).</p><p><strong>Materials and methods: </strong>This study is experimental-based and was designed using a six-group treatment method, namely normal control (KN: not treated by MNU, nanodiamond (ND), or Q); negative control (K-: treated by MNU); positive control (K+: treated by MNU and capecitabine); ND (treated by MNU and NDs); Q (treated by MNU and Q); and NDQ (treated by MNU and NDQ). To induce colon cancer in rats, MNU (10 mg/Kg BW) was administrated intrarectally three times per week for four weeks. The treatment of Q (40 mg/Kg BW) or NDQ (40 mg/Kg BW) was given intraperitoneally twice a week for 6 weeks. Cancer progression of all cohorts was evaluated by performing body and colon weight measurements, which involved the following: ELISA assay-specific to metastatic marker matrix metalloprotein-9 (MMP-9), carcinoembryonic antigen (CEA), hypoxia-inducible factor 1 α (HIF1α), vascular endothelial growth factor, protein 53 (p53) and immunohistochemistry staining of Caspase-3 and Ki-67 proteins. Observation of cancer metastasis to the lung was also performed.</p><p><strong>Results: </strong>NDQ significantly inhibited cancer aggressiveness by causing an increment in body weight gain and the growth rate-while reducing the colon weight compared to the K- group. Moreover, decreased levels of MMP-9, CEA, HIF-1α, and Ki67 and increased levels of p53 and Caspase-3 were more significant in the NDQ group than in the Q group. The lung tumor metastases in the NDQ group were fewer than in the K- group.</p><p><strong>Conclusion: </strong>NDQ increased Q's anticancer activity, suggesting that NDs have an effective drug delivery property.</p>","PeriodicalId":94363,"journal":{"name":"Turkish journal of biology = Turk biyoloji dergisi","volume":"48 5","pages":"279-289"},"PeriodicalIF":0.0,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11518354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disappearance of Cdc14 from the daughter spindle pole body requires Glc7-Bud14.","authors":"İdil Kirdök, Ayşe Kosa Çaydaşi","doi":"10.55730/1300-0152.2707","DOIUrl":"10.55730/1300-0152.2707","url":null,"abstract":"<p><strong>Background/aim: </strong>The conserved phosphatase Cdc14 facilitates mitotic exit in budding yeast by counteracting mitotic cyclin-dependent kinase activity. Cdc14 is kept in the nucleolus until anaphase onset, when it is released transiently into the nucleoplasm. In late anaphase, Cdc14 is fully released into the cytoplasm upon activation of the mitotic exit network (MEN) to trigger mitotic exit. Cdc14 also localizes to yeast spindle pole bodies (SPBs) in anaphase and dephosphorylates key targets residing on SPBs to allow SPB duplication and prime the MEN. Protein phosphatase 1 (Glc7) with regulatory subunit Bud14 is another phosphatase that plays a key role in the spatiotemporal control of mitotic exit. In this study, we investigated the regulation of Cdc14 localization by Bud14-Glc7.</p><p><strong>Materials and methods: </strong>We used fluorescence microscopy to analyze Cdc14 localization in <i>BUD14</i> wildtype and <i>BUD14</i> knockout cells (<i>bud14Δ</i>) as well as in cells expressing a mutant allele of <i>BUD14</i> (<i>bud14-F379A</i>) that cannot bind Glc7. We also utilized a yeast two-hybrid (Y2H) system to examine the interaction of Bud14 with Cdc14.</p><p><strong>Results: </strong>We found that Cdc14 remains at the SPBs longer in <i>bud14Δ</i> and <i>bud14-F379A</i> compared to wildtype cells. This effect is limited to the SPB that has migrated to the daughter cell (dSPB). Cdc14 localizes to both SPBs shortly after anaphase onset. In mid-to-late anaphase, levels of Cdc14 increase at the dSPB in both wildtype and <i>bud14Δ</i> cells. With mitotic exit, Cdc14 disappears from the dSPB in wildtype cells but not in <i>bud14Δ</i> cells. Accordingly, 50% of <i>bud14Δ</i> cells in G1 have Cdc14 at their SPBs. We also found that Cdc14 localization at the dSPB was largely, but not entirely, dependent on Bfa1 in <i>bud14Δ</i> cells. Furthermore, Bud14 interacted with Cdc14 in the Y2H system.</p><p><strong>Conclusion: </strong>Our results suggest that Glc7-Bud14 is part of a mechanism that promotes Cdc14 disappearance from the dSPB.</p>","PeriodicalId":94363,"journal":{"name":"Turkish journal of biology = Turk biyoloji dergisi","volume":"48 5","pages":"308-318"},"PeriodicalIF":0.0,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11518377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Delivery of <i>BikDD</i> proapoptotic gene in Peptide-18-targeted Poly(2-oxazoline)-DOPE nanoliposomes for breast cancer models.","authors":"Zeynep Büşra Bolat, Ayça Ece Nezir, Ongun Mehmet Saka, Itır Ebru Zemheri, Sevgi Gülyüz, Umut Uğur Özköse, Özgür Yilmaz, Asuman Bozkir, Dilek Telci, Fikrettin Şahin","doi":"10.55730/1300-0152.2706","DOIUrl":"https://doi.org/10.55730/1300-0152.2706","url":null,"abstract":"<p><p>Breast cancer is one of the most common cancers and a significant cause of death in females worldwide. For effective breast cancer treatment, using systems with a promising delivery of anticancer agents is an important strategy. Peptide 18 (P18), a tumor-homing peptide, shows a high binding affinity toward breast cancer cells. Nanoliposomes are known to have enhanced accumulation ability in tumors with longer systemic circulation. In this study, Poly (2-ethyl-2-oxazoline) (PEtOx) polymers conjugated with DOPE are used to prepare PEtOx-DOPE nanoliposomes. <i>BikDD</i>, a mutant form of the Bik gene and a member of the BH3-only proapoptotic genes, mimics the constitutively phosphorylated form of the gene. To the best of our knowledge, this study presents a novel approach by investigating P18-conjugated PEtOx-DOPE nanoliposomes (P18-PEtOx-DOPE) for the targeted delivery of <i>BikDD</i> to the AU565 breast cancer model. A site-directed mutated <i>BikDD</i> was loaded into P18-PEtOx-DOPE nanoliposomes, and the targeted drug delivery system was assessed in in vitro and in vivo breast cancer models for efficiency, safety, and efficacy. The increased Bik mRNA expression levels in AU565 cells suggest a high effectiveness of the targeting PEtOx-DOPE nanoliposomes. Following the in vitro studies, the delivery of <i>BikDD</i> by P18-PEtOx-DOPE nanoliposomes was analyzed in CD-1 nude mice models. The animal study showed no significant difference in the tumor volume of the CD-1 nude mice treated with P18-PEtOx-DOPE-BikDD nanoliposomes compared to the free delivery of <i>BikDD</i>. Our preclinical studies suggest that P18-PEtOx-DOPE-BikDD nanoliposomes may be promising gene carriers for targeted breast cancer therapy. Thus, further studies should be carried out to determine the prolonged use of this drug delivery system in breast cancer therapy.</p>","PeriodicalId":94363,"journal":{"name":"Turkish journal of biology = Turk biyoloji dergisi","volume":"48 5","pages":"299-307"},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11518345/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pterostilbene suppresses head and neck cancer cell proliferation via induction of apoptosis.","authors":"Talih Özdaş, Sibel Özdaş, İpek Canatar, Erdem Kaypak","doi":"10.55730/1300-0152.2708","DOIUrl":"https://doi.org/10.55730/1300-0152.2708","url":null,"abstract":"<p><strong>Background/aim: </strong>Head and neck cancer (HNC) is one of the most prevalent causes of death worldwide, and so discovering anticancer agents for its treatment is very important. Pterostilbene (PS) is a trans-stilbene reported to be beneficial in managing various cancers. The objective of the study was to evaluate the cytotoxic, antiproliferative, proapoptotic, and antimigrative effect of PS on HEp-2, SCC-90, SCC-9, FaDu, and Detroit-551 cell lines.</p><p><strong>Materials and methods: </strong>MTT and live/dead assays were employed to assess cell viability, while a cell migration test was performed to evaluate wound healing capacity. The mRNA, protein, and intracellular expression levels of <i>CASP-3</i>, <i>BAX</i>, and <i>BCL-2</i> genes were evaluated by real-time PCR, western blotting, and immunofluorescence staining. Annexin V-PI staining was conducted to assess the amounts of viable, apoptotic, and necrotic cells.</p><p><strong>Results: </strong>The results revealed that PS displayed cytotoxic, antiproliferative activity in a dose-dependent manner in HNC cells by upregulating <i>CASP-3</i> and <i>BCL-2</i> while downregulating <i>BCL-2</i> in the apoptotic pathway. The proapoptotic properties were confirmed by the annexin-V-IP results. Moreover, PS displayed a significant suppressive efficacy on the migration capacity of HNC cells.</p><p><strong>Conclusion: </strong>The present study provides proof that PS has the prospective to be improved as an attractive anticancer agent against HNC following advanced studies.</p>","PeriodicalId":94363,"journal":{"name":"Turkish journal of biology = Turk biyoloji dergisi","volume":"48 5","pages":"319-337"},"PeriodicalIF":0.0,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11518375/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optical imaging and gene transfection potential of linear polyethylenimine-coated Ag₂S near-infrared quantum dots.","authors":"Altay Savalan","doi":"10.55730/1300-0152.2709","DOIUrl":"https://doi.org/10.55730/1300-0152.2709","url":null,"abstract":"<p><strong>Background/aim: </strong>The application of biocompatible heavy metal-free and cationic Ag₂S NIR quantum dots (QDs), which have intense luminosity in the 700-900 nm medical range, as a nonviral gene delivery system paves the way to overcome autofluorescence and easily track the delivery of genes in real time.</p><p><strong>Materials and methods: </strong>The newly developed small and colloidally stable 2-mercaptopropionic acid (MPA)-capped Ag₂S aqueous quantum dots electrostatically complexed with linear polyethyleneimine (Ag₂S@2MPA/LPEI) were investigated for the first time both as a strong fluorescent probe and as a vector for nonviral gene delivery for the highest tracking of the system and delivery of genes into the nuclei of different cancer cells. The synthesized Ag₂S@2MPA/LPEI quantum dots demonstrated strong optical imaging properties and were used to deliver a green fluorescent protein (GFP) plasmid as a standard gene.</p><p><strong>Results: </strong>For Ag₂S@2MPA/LPEI-pDNA nanoparticles, an N/P ratio of 20 was the ideal transfection efficiency. Ag₂S@2MPA/LPEI was substantially more compatible with HEK 293T cells than the free 25-kDa linear polyethylenimine (LPEI). Next, the transfection efficiency evaluation of pGFP genes with synthesized Ag₂S@2MPA/LPEI and LPEI in different cancer cells demonstrated their high potential as a theranostic cancer gene delivery system.</p><p><strong>Conclusion: </strong>This is the first instance of gene transfection and optical imaging carried out in vitro using Ag₂S@2MPA/LPEI QDs. Overall, the newly synthesized highly biocompatible and trackable Ag₂S@2MPA/LPEI QDs can be an effective and biocompatible theranostic system for cancer gene therapy.</p>","PeriodicalId":94363,"journal":{"name":"Turkish journal of biology = Turk biyoloji dergisi","volume":"48 5","pages":"338-347"},"PeriodicalIF":0.0,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11518328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bud14 function is crucial for spindle pole body size maintenance.","authors":"Sevilay Münire Girgin, Ayşe Koca Çaydaşi","doi":"10.55730/1300-0152.2702","DOIUrl":"10.55730/1300-0152.2702","url":null,"abstract":"<p><strong>Background/aim: </strong>Spindle pole bodies (SPB), the functional equivalent of centrosomes in yeast, duplicate through generation of a new SPB next to the old one. However, SPBs are dynamic structures that can grow and exchange, and mechanisms that regulate SPB size remain largely unknown. This study aims to elucidate the role of Bud14 in SPB size maintenance in <i>Saccharomyces cerevisiae</i>.</p><p><strong>Materials and methods: </strong>We employed quantitative fluorescence microscopy to assess the relative and absolute amounts of SPB structural proteins at SPBs of wildtype cells and in cells lacking <i>BUD14</i> (<i>bud14Δ</i>). Quantifications were performed using asynchronous cell cultures, as well as cultures synchronously progressing through the cell cycle and upon different cell cycle arrests. We also utilized mutants that allow the separation of Bud14 functions.</p><p><strong>Results: </strong>Our results indicate that higher levels of SPB inner, outer, and central plaque proteins are present at the SPBs of <i>bud14Δ</i> cells compared to wildtype cells during anaphase, as well as during nocodazole-induced M-phase arrest. However, during α-factor mediated G1 arrest, inner and outer plaque proteins responded differently to the absence of <i>BUD14</i>. A Bud14 mutant that cannot interact with the Protein Phosphatase 1 (Glc7) phenocopied <i>bud14Δ</i> in terms of SPB-bound levels of the inner plaque protein Spc110, whereas disruption of Bud14-Kel1-Kel2 complex did not alter Spc110 levels at SPBs. In cells synchronously released from α-factor arrest, lack of Bud14-Glc7 caused increase of Spc110 at the SPBs at early stages of the cell cycle.</p><p><strong>Conclusion: </strong>We identified Bud14 as a critical protein for SPB size maintenance. The interaction of Bud14 with Glc7, but not with the Kelch proteins, is indispensable for restricting levels of Spc110 incorporated into the SPBs.</p>","PeriodicalId":94363,"journal":{"name":"Turkish journal of biology = Turk biyoloji dergisi","volume":"48 4","pages":"267-278"},"PeriodicalIF":0.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11407341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142305492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction of Griscelli Syndrome Type 2 causing mutations in the <i>RAB27A</i> gene with CRISPR/Cas9.","authors":"Özgür Doğuş Erol, Şimal Şenocak, Burcu Özçimen, Gülen Güney Esken, Hasan Basri Kiliç, Çetin Kocaefe, Niek P VAN Til, Fatima Aerts Kaya","doi":"10.55730/1300-0152.2705","DOIUrl":"https://doi.org/10.55730/1300-0152.2705","url":null,"abstract":"<p><strong>Background/aim: </strong>Griscelli Syndrome Type 2 (GS-2) is a rare, inherited immune deficiency caused by a mutation in the <i>RAB27A</i> gene. The current treatment consists of hematopoietic stem cell transplantation, but a lack of suitable donors warrants the development of alternative treatment strategies, including gene therapy. The development of mutation-specific clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 gene editing technology has opened the way for custom-designed gene correction of patient-derived stem cells. In this study, we aimed to custom design CRISPR/Cas9 constructs and test their efficiency on homology-directed repair (HDR) on the correction of exon 3 and exon 7 mutations in the <i>RAB27A</i> gene of GS-2 patient-derived mesenchymal stem cells (MSCs) and induced pluripotent stem cells.</p><p><strong>Materials and methods: </strong>We assessed <i>RAB27A</i> gene and protein expression using qRT-PCR, Western Blot, and immune fluorescence in GS-2 patient-derived MSCs and induced pluripotent stem cells (iPSCs). Guide RNAs (gRNAs) and donor DNAs were designed based on patient mutations in exon 3 and exon 7 using the CHOPCHOP online tool and transfected into GS-2 MSCs and iPSCs by electroporation. The cells were cultured for 2 days and then used for mutation analysis using DNA sequencing.</p><p><strong>Results: </strong>MSCs and iPSCs from the GS-2 patients lacked <i>RAB27A</i> gene and protein expression. After gRNA and donor DNAs were designed and optimized, we found HDR efficiency with gRNA3.3 (10% efficiency) and gRNA7.3 (27% efficiency) for MSCs but lower efficiency in iPSCs (<5%). However, transfection of both MSCs and iPSCs resulted in massive cell death, loss of colony formation, and spontaneous differentiation.</p><p><strong>Conclusion: </strong>The use of CRISPR/Cas9 to genetically correct MSCs and iPSCs from GS-2 patients with different mutations through HDR is feasible but requires optimization of the procedure to reduce cell death and improve stem cell function before clinical application.</p>","PeriodicalId":94363,"journal":{"name":"Turkish journal of biology = Turk biyoloji dergisi","volume":"48 5","pages":"290-298"},"PeriodicalIF":0.0,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11518329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expressions of the satellite repeat HSAT5 and transposable elements are implicated in disease progression and survival in glioma.","authors":"Sıla Naz Köse, Tutku Yaraş, Ahmet Bursali, Yavuz Oktay, Cihangir Yandim, Gökhan Karakülah","doi":"10.55730/1300-0152.2700","DOIUrl":"https://doi.org/10.55730/1300-0152.2700","url":null,"abstract":"<p><p>The glioma genome encompasses a complex array of dysregulatory events, presenting a formidable challenge in managing this devastating disease. Despite the widespread distribution of repeat and transposable elements across the human genome, their involvement in glioma's molecular pathology and patient survival remains largely unexplored. In this study, we aimed to characterize the links between the expressions of repeat/transposable elements with disease progression and survival in glioma patients. Hence, we analyzed the expression levels of satellite repeats and transposons along with genes in low-grade glioma (LGG) and high-grade glioma (HGG). Endogenous transposable elements LTR5 and HERV_a-int exhibited higher expression in HGG patients, along with immune response-related genes. Altogether, 16 transposable elements were associated with slower progression of disease in LGG patients. Conversely, 22 transposons and the HSAT5 satellite repeat were linked to a shorter event-free survival in HGG patients. Intriguingly, our weighted gene coexpression network analysis (WGCNA) disclosed that the HSAT5 satellite repeat resided in the same module network with genes implicated in chromosome segregation and nuclear division; potentially hinting at its contribution to disease pathogenesis. Collectively, we report for the first time that repeat and/or transposon expression could be related to disease progression and survival in glioma. The expressions of these elements seem to exert a protective effect during LGG-to-HGG progression, whereas they could have a detrimental impact once HGG is established. The results presented herein could serve as a foundation for further experimental work aimed at elucidating the molecular regulation of glioma genome.</p>","PeriodicalId":94363,"journal":{"name":"Turkish journal of biology = Turk biyoloji dergisi","volume":"48 4","pages":"242-256"},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11407350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142305494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the impact of diabetes on aging: insights from TERT and COL1A1 methylation.","authors":"Jessica Nathania Liamri, Farizky Martriano Humardani, Giovani Chandra, Lisa Thalia Mulyanata, Tjie Kok, Fenny Irawati, Hikmawan Wahyu Sulistomo, Christoph Reichetzeder, Sulistyo Emantoko Dwi Putra","doi":"10.55730/1300-0152.2701","DOIUrl":"https://doi.org/10.55730/1300-0152.2701","url":null,"abstract":"<p><strong>Background/aim: </strong>Aging, a multifaceted biological process, leads to diminished physical performance, especially in older adults with diabetes, where a mismatch between biological and chronological age is noticeable. Numerous studies have demonstrated that diabetes accelerates aging at the cellular and organ levels. Notable aging markers are telomerase reverse transcriptase (TERT), related to telomere length, and type 1 chain collagen (COL1A1), a key component of skin collagen. Additionally, age-related methylation increases, as revealed through methylation analysis, augmenting aspects of aging. However, the detailed interplay between aging and diabetes, particularly regarding methylation, remains underexplored and warrants further study to elucidate the biological links between the two.</p><p><strong>Materials and methods: </strong>In this study, we elucidate the modulatory influence of diabetes on the aging process, focusing specifically on the modifications in TERT in the kidney and COL1A1 in the skin using mice of Swiss Webster strain as the diabetes model. Specimens were categorized into three distinct chronological cohorts: chronologically young (16 weeks; n = 5), chronologically old (40 weeks; n = 5), and a periodically assessed group (16 weeks; n = 30), from which five mice were systematically sacrificed on a weekly basis.</p><p><strong>Results: </strong>Our findings reveal a marked impact of diabetes on the methylation statuses of TERT and COL1A1, characterized by an elevation in methylation levels within the periodic group (1st-6th week) and a simultaneous, progressive attenuation in the expression of TERT and COL1A1 genes.</p><p><strong>Conclusion: </strong>The observed alterations in the methylation levels of TERT and COL1A1 propound the hypothesis that diabetes potentially expedites the aging process, concomitantly impinging on the production of TERT and COL1A, ostensibly through the mechanism of promoter gene hypermethylation.</p>","PeriodicalId":94363,"journal":{"name":"Turkish journal of biology = Turk biyoloji dergisi","volume":"48 4","pages":"257-266"},"PeriodicalIF":0.0,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11407328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142305493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anatolian medicinal plants as potential antiviral agents: bridging traditional knowledge and modern science in the fight against COVID-19 and related viral infections.","authors":"Engin Tilkat, Israt Jahan, Ayşe Hoşer, Alevcan Kaplan, Oğuzhan Özdemir, Ahmet Onay","doi":"10.55730/1300-0152.2699","DOIUrl":"https://doi.org/10.55730/1300-0152.2699","url":null,"abstract":"<p><p>The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was the cause of the coronavirus 2019 (COVID-19), commonly known as the coronavirus pandemic. Since December 2020, COVID-19 vaccines have been extensively administered in numerous countries. In addition to new antiviral medications, the treatment regimen encompasses symptom management. Despite sustained research efforts, the outbreak remains uncontrolled, with affected patients still lacking proper treatment. This review is a valuable asset for researchers and practitioners aiming to delve into the yet unexplored potential of Anatolian flora in the fight against COVID-19 and other viral infections. Numerous medicinal plants in Anatolia, such as thyme, sage, cannabis, oregano, licorice root, and <i>Origanum</i> sp., contain bioactive compounds with proven antiviral properties that have been used in the region for centuries. The rich legacy of traditional Anatolian medicine (TAM), has significantly influenced modern medicine; thus, the profusion of medicinal plants native to Anatolia holds promise for antiviral drug development, making this review essential for researchers and practitioners.</p>","PeriodicalId":94363,"journal":{"name":"Turkish journal of biology = Turk biyoloji dergisi","volume":"48 4","pages":"218-241"},"PeriodicalIF":0.0,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11407354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142305491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}