Journal of pharmaceutical analysis最新文献_第9页

UGP system: A deep learning-driven platform for automated identification of ultrafine granular powders using chromatographic fingerprinting. UGP系统：一个深度学习驱动的平台，用于使用色谱指纹图谱自动识别超细颗粒粉末。

IF 8.9

Journal of pharmaceutical analysis Pub Date : 2026-05-01 Epub Date: 2025-10-22 DOI: 10.1016/j.jpha.2025.101474

Fei Huang, Ya-Ling An, Li-Jie Zhang, Jia-Wei Wang, Ming-Jin Zhang, Zhen-Wei Li, Xiao-Kang Liu, Dai-di Zhang, Qian-Liang Zhang, Li-Hua Peng, Wei-Lin Qiao, De-An Guo

{"title":"UGP system: A deep learning-driven platform for automated identification of ultrafine granular powders using chromatographic fingerprinting.","authors":"Fei Huang, Ya-Ling An, Li-Jie Zhang, Jia-Wei Wang, Ming-Jin Zhang, Zhen-Wei Li, Xiao-Kang Liu, Dai-di Zhang, Qian-Liang Zhang, Li-Hua Peng, Wei-Lin Qiao, De-An Guo","doi":"10.1016/j.jpha.2025.101474","DOIUrl":"10.1016/j.jpha.2025.101474","url":null,"abstract":"This study developed an intelligent identification system for ultrafine granular powder (UGP) by integrating high performance liquid chromatography (HPLC) fingerprinting with deep learning algorithms. A comprehensive HPLC fingerprint database encompassing 530 batches from 53 UGP varieties across 29 botanical families was established using a standardized 60-min, six-wavelength detection protocol (210, 230, 254, 280, 327, and 380 nm). Chromatographic reproducibility was ensured with quality control (QC) sample retention time relative standard deviations (RSDs) below 2%. A three-layer one-dimensional convolutional neural network (1D-CNN) was designed with 32, 64, and 128 filters in successive layers for species classification. Data augmentation techniques including noise interference, baseline drift, and retention time shifts (3.5-60 min) expanded the dataset sixfold and enhanced model generalization capabilities. The optimized model achieved excellent performance on test data with 97.62% accuracy, 97.97% precision, and 97.16% recall, demonstrating consistent reproducibility with mean accuracy of 97.2% ± 0.65% across ten independent training runs. External validation using 63 commercial samples yielded 95.24% identification accuracy, confirming practical applicability. The Flask-based web system enables automated workflows from data upload to species identification and is accessible to users without specialized expertise. This work establishes a standardized approach for intelligent authentication of food-medicine homologous Chinese medicinal UGPs, addressing regulatory and consumer requirements for product authenticity and safety in pharmaceutical and functional food industries.","PeriodicalId":94338,"journal":{"name":"Journal of pharmaceutical analysis","volume":"16 5","pages":"101474"},"PeriodicalIF":8.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13217488/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148140532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA-encoded library screening identifies CDK2-targeting lead compounds with favorable drug-like properties for anticancer development. dna编码文库筛选鉴定cdk2靶向先导化合物具有良好的抗肿瘤药物样特性。

IF 8.9

Journal of pharmaceutical analysis Pub Date : 2026-05-01 Epub Date: 2025-11-25 DOI: 10.1016/j.jpha.2025.101498

Li Zhou, Yong Ju, Zhijuan Cao, Sheng Cai, Jiayuan Su, Jianzhong Lu

引用次数: 0

¹⁹F-{¹H} NMR spectroscopy in weakly orienting solvents for the enantiomeric resolution of fluorinated chiral drugs: The case of fluoxetine. 弱取向溶剂中用于氟化手性药物对映体拆分的19F-{1H} NMR波谱：氟西汀的例子。

IF 8.9

Journal of pharmaceutical analysis Pub Date : 2026-05-01 Epub Date: 2025-10-10 DOI: 10.1016/j.jpha.2025.101469

Vincent Chiapolino, François-Marie Moussallieh, Philippe Lesot, Boris Gouilleux

{"title":"19F-{1H} NMR spectroscopy in weakly orienting solvents for the enantiomeric resolution of fluorinated chiral drugs: The case of fluoxetine.","authors":"Vincent Chiapolino, François-Marie Moussallieh, Philippe Lesot, Boris Gouilleux","doi":"10.1016/j.jpha.2025.101469","DOIUrl":"10.1016/j.jpha.2025.101469","url":null,"abstract":"A rapid and simple method combining 19F-{1H} nuclear magnetic resonance (NMR) and weakly orienting chiral solvents is proposed for the spectral discrimination and accurate quantitation of fluoxetine (FLX) enantiomers. FLX is a well-known bioactive chiral drug in pharmacology with well-established anti-depressant properties (Prozac or Sarafem) and currently used as a racemate. Since 1991, it has been established that the (S)-form of FLX shows different pharmacokinetic and pharmacodynamic profiles in comparison to the (R)-form, hence the development of novel enantioresolved NMR methods for analyzing FLX is of interest. The reported approach, relying on a commercial polymer, poly-γ-benzyl-L-glutamate (PBLG) as chiral selector, addresses the enantiomeric analysis of FLX hydrochloride solutions with a high level of accuracy in a short time. The influence of experimental parameters, such as solute concentration and temperature on the enantiomeric resolution, is investigated and discussed in depth. In particular, the uniformity and stability of PBLG-based lyotropic liquid crystals (LLCs) in presence of a hydrochloride analyte is assessed for the first time by 19F NMR imaging. All the outcomes obtained highlight the analytical potential of this NMR approach for the enantiomeric analysis of fluorinated chiral drugs while the spectral data recorded at various conditions in temperature and mesophase composition provide new valuable insights toward a better understanding of chiral recognition processes in polypeptide orienting media.","PeriodicalId":94338,"journal":{"name":"Journal of pharmaceutical analysis","volume":"16 5","pages":"101469"},"PeriodicalIF":8.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13193786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148019036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of NRF2 in human cancers: Pre-clinical insights paving the way for clinical trials. NRF2在人类癌症中的作用：为临床试验铺平道路的临床前见解。

IF 8.9

Journal of pharmaceutical analysis Pub Date : 2026-05-01 Epub Date: 2025-06-02 DOI: 10.1016/j.jpha.2025.101358

Yi Pei, Jianqiao Yin, Jiamei Liu, Dongze Liu, Qianlong Wu, Xue Cai, Mingming Han, Yu Tian, Liyu Yang, Shengye Liu

{"title":"The role of NRF2 in human cancers: Pre-clinical insights paving the way for clinical trials.","authors":"Yi Pei, Jianqiao Yin, Jiamei Liu, Dongze Liu, Qianlong Wu, Xue Cai, Mingming Han, Yu Tian, Liyu Yang, Shengye Liu","doi":"10.1016/j.jpha.2025.101358","DOIUrl":"10.1016/j.jpha.2025.101358","url":null,"abstract":"Tumorigenesis is considered as a complex, multistep process in which genetic mutations play a crucial role. The genetic mutations cause notable changes, not only in the biological behavior of tumor cells but also in their reactions to treatment. Nuclear factor erythroid 2-related factor 2 (NRF2) is one of the most disrupted molecular pathways in human cancers, and during cancer development, the expression of this factor rises to enhance survival rates. The NRF2 seems crucial for shielding tumor cells from apoptosis and oxidative damage, while promoting pro-survival autophagy to increase the survival rate. Crucially, NRF2 plays a dual role in enhancing both the growth and metastasis of cancer cells, and the upregulation of this factor boosts the stemness and cancer-stem cell characteristics of tumor cells, while also promoting drug resistance and radioresistance. The elevation of glycolysis, activation of epithelial-mesenchymal transition (EMT), and inhibition of ferroptosis are additional features of NRF2 upregulation in human cancers. Among the different pathways that control NRF2, non-coding RNA transcripts play a significant role, and by altering NRF2 expression, they influence tumor development. The pharmacological modulation of NRF2 can occur through both direct and indirect methods; in the direct method, NRF2 is inhibited, whereas in the indirect method, the regulators and associated pathways of NRF2, such as Kelch-like ECH-associated protein 1 (KEAP1), are influenced. The nanoparticles have been engineered to inhibit NRF2 in decreasing tumorigenesis. Consequently, the clinical application of current discoveries can enhance cancer treatment capabilities for patients in the near future.","PeriodicalId":94338,"journal":{"name":"Journal of pharmaceutical analysis","volume":"16 5","pages":"101358"},"PeriodicalIF":8.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13199830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148038803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraction and determination of sartans: Recent advances and analytical perspectives. 沙坦的提取和测定：最新进展和分析观点。

IF 8.9

Journal of pharmaceutical analysis Pub Date : 2026-05-01 Epub Date: 2025-10-21 DOI: 10.1016/j.jpha.2025.101472

Peng-Li Wei, Yuan Zhang, Cheng Du, Xue-Song Feng, Xiao-Dan Liu

{"title":"Extraction and determination of sartans: Recent advances and analytical perspectives.","authors":"Peng-Li Wei, Yuan Zhang, Cheng Du, Xue-Song Feng, Xiao-Dan Liu","doi":"10.1016/j.jpha.2025.101472","DOIUrl":"10.1016/j.jpha.2025.101472","url":null,"abstract":"Sartans are essential antihypertensive drugs requiring reliable analytical methods for quality and efficacy monitoring. This review provides a comprehensive overview of recent analytical advances for sartans, focusing on pretreatment and detection methods. For sample preparation, conventional methods like protein precipitation, liquid-liquid extraction (LLE) and solid-phase extraction (SPE) have been optimized through the adoption of green solvents and automation. Emerging novel microextraction techniques including liquid phase microextraction and solid phase microextraction, in combination with advanced materials improve selectivity, especially for complex biological matrices. In the realm of detection, liquid chromatography-mass spectrometry (LC-MS) remains dominant, with high-resolution mass spectrometry (HRMS) (time-of-flight, orbitrap) enabling trace-level quantification and structural identification, outperforming traditional low-resolution mass spectrometry (LRMS). Emerging sensors offer rapid screening but lack MS-level multiplexing capability. The choice of methods should be based on sample complexity and analytical needs: biological samples benefit from microextraction-HRMS combinations, whereas pharmaceutical analysis may use simpler SPE-LC/MS workflows. Future directions should emphasize miniaturized, automated, and eco-friendly approaches to enhance throughput while reducing environmental impact. This review serves as a practical guide for selecting suitable strategies for the analysis of sartans across diverse analytical scenarios.","PeriodicalId":94338,"journal":{"name":"Journal of pharmaceutical analysis","volume":"16 5","pages":"101472"},"PeriodicalIF":8.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13217482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148140495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking Wnt's weak spot: Glycosylated nanoalbumins to reignite immune responses in MSS-CRC. 解锁Wnt的弱点：糖基化纳米白蛋白重新点燃MSS-CRC的免疫反应。

IF 8.9

Journal of pharmaceutical analysis Pub Date : 2026-05-01 Epub Date: 2025-07-24 DOI: 10.1016/j.jpha.2025.101412

Xin Wei, Mingzhu Zuo, Qiongwen Liang, Shiwei Zhang, Jingmei Wang, Zhanfeng Li, Wenguang Yang, Fang Ma, Wangxiao He, Tianya Liu

{"title":"Unlocking Wnt's weak spot: Glycosylated nanoalbumins to reignite immune responses in MSS-CRC.","authors":"Xin Wei, Mingzhu Zuo, Qiongwen Liang, Shiwei Zhang, Jingmei Wang, Zhanfeng Li, Wenguang Yang, Fang Ma, Wangxiao He, Tianya Liu","doi":"10.1016/j.jpha.2025.101412","DOIUrl":"10.1016/j.jpha.2025.101412","url":null,"abstract":"Microsatellite-stable colorectal cancer (MSS-CRC) is characterized by poor immune infiltration and immune evasion, leading to rapid tumor progression and limited efficacy of current immunotherapies. The bioinformatics analysis revealed that the hyperactivation of the Wnt/β-catenin signaling pathway in MSS-CRC is instrumental in mediating immune suppression. Although inhibiting this pathway presents a therapeutic opportunity, no Wnt inhibitors have been clinically approved due to Wnt's essential role in maintaining tissue homeostasis, with inhibition in normal cells causing significant toxicity. To address it, we discovered that Wnt activation in colorectal cancer cells enhances macropinocytosis, particularly favoring the uptake of glycosylated proteins to meet increased nutrient demands. Building on this insight, we developed a glycosylated human serum albumin (GHSA) co-assembled with carnosic acid (CA), termed glycosylated human serum albumin-carnosic acid (GHSACA), which is selectively internalized by Wnt-activated colorectal cancer cells. This approach not only reduces off-target toxicity but also effectively inhibits the Wnt pathway, resulting in notable tumor inhibition and immune reactivation in murine models, while maintaining a favorable safety profile. This strategy offers a promising therapeutic solution by combining selective Wnt inhibition with enhanced immune activation in MSS-CRC, and highlights the potential of leveraging disease-specific cellular uptake mechanisms for designing nanomedicines, advancing the development of precision-targeted cancer therapies.","PeriodicalId":94338,"journal":{"name":"Journal of pharmaceutical analysis","volume":"16 5","pages":"101412"},"PeriodicalIF":8.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13156595/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147871226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polydatin for treating spinal cord injury: Multiple mechanisms and challenges. 多肽治疗脊髓损伤：多种机制和挑战。

IF 8.9

Journal of pharmaceutical analysis Pub Date : 2026-04-01 Epub Date: 2025-09-15 DOI: 10.1016/j.jpha.2025.101451

Zhishuo Wang, Jiaming Zhang, Longyu Li, Yuhao Zhang, Haoyu Shen, Chunfeng Shang, Zikuan Leng, Guowei Shang, Hongwei Kou, Keya Mao, Hao Han, Songfeng Chen, Hongjian Liu

{"title":"Polydatin for treating spinal cord injury: Multiple mechanisms and challenges.","authors":"Zhishuo Wang, Jiaming Zhang, Longyu Li, Yuhao Zhang, Haoyu Shen, Chunfeng Shang, Zikuan Leng, Guowei Shang, Hongwei Kou, Keya Mao, Hao Han, Songfeng Chen, Hongjian Liu","doi":"10.1016/j.jpha.2025.101451","DOIUrl":"10.1016/j.jpha.2025.101451","url":null,"abstract":"Spinal cord injury (SCI) is a serious neurological system disease. After SCI, a series of cascade reactions can cause irreversible damage, with a high disability rate and mortality rate. The complexity of the pathological mechanism of SCI limits the efficacy of various traditional therapies, and it is urgent to find new therapeutic means. In recent years, the method of extracting effective components from natural Chinese herbs for treating diseases has attracted widespread attention. Polydatin (PD) is an active ingredient extracted from Polygonum cuspidatum and its structure is similar to the traditional drug resveratrol. Sufficient studies have proved that PD plays anti-inflammatory, antioxidant, anti-apoptotic and neuroprotective roles in the treatment of multisystem diseases. These effects are also significant in the treatment of SCI. Based on the structural differences between PD and resveratrol, this paper illustrates the feasibility of PD in the treatment of SCI, and systematically expounds the pathophysiological process of SCI and the molecular mechanism of PD in the treatment of SCI. Furthermore, this article discusses feasible measures to improve the bioavailability of PD, summarizes the application of new drug delivery systems in PD, and analyzes the challenges and prospects of PD in SCI treatment.","PeriodicalId":94338,"journal":{"name":"Journal of pharmaceutical analysis","volume":"16 4","pages":"101451"},"PeriodicalIF":8.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13098523/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PFKM promotes chemoresistance in lung adenocarcinoma by regulating RAB8B mediated exosome release. PFKM通过调节RAB8B介导的外泌体释放促进肺腺癌的化疗耐药。

IF 8.9

Journal of pharmaceutical analysis Pub Date : 2026-04-01 Epub Date: 2025-09-12 DOI: 10.1016/j.jpha.2025.101450

Qiang Wang, Qiyao Nong, Junguo Zang, Meiyu Gao, Ying Zhang, Xinyuan Hao, Yuan Tian, Fengguo Xu, Pei Zhang

{"title":"PFKM promotes chemoresistance in lung adenocarcinoma by regulating RAB8B mediated exosome release.","authors":"Qiang Wang, Qiyao Nong, Junguo Zang, Meiyu Gao, Ying Zhang, Xinyuan Hao, Yuan Tian, Fengguo Xu, Pei Zhang","doi":"10.1016/j.jpha.2025.101450","DOIUrl":"10.1016/j.jpha.2025.101450","url":null,"abstract":"Lung adenocarcinoma (LUAD), the most widely existing subtype of non-small cell lung cancer (NSCLC), is a leading cause of cancer-related mortality, characterized by challenging early diagnosis, high rates of recurrence and metastasis, and poor prognosis. Chemotherapy remains the primary treatment for advanced LUAD, but its effectiveness is often hindered by the development of chemoresistance. In this study, a targeted metabolomics method unveiled a marked up-regulation of glycolysis in chemotherapy-resistant LUAD cells. Particularly, the ratio of fructose 1,6-bisphosphate (FBP) to fructose 6-phosphate (F6P) reflected the activity of the rate-limiting enzyme phosphofructokinase muscle isoform (PFKM) was significantly elevated. We further observed a significant increase in exosome release in chemotherapy-resistant cells. More importantly, it was found that the interaction between PFKM and exosomes plays a role in regulating chemoresistance in LUAD. Mechanistically, PFKM influences exosomes release by modulating Ras-related protein Rab-8B (RAB8B) expression, impacting apoptosis and glycolytic metabolism, thereby promoting chemoresistance. Furthermore, drug-resistant cells enhance chemoresistance in sensitive cells by releasing exosomes with heightened glycolytic activity. These findings highlight the crucial role of the PFKM-RAB8B axis in promoting chemoresistance, suggesting it as a potential therapeutic target for countering LUAD chemoresistance.","PeriodicalId":94338,"journal":{"name":"Journal of pharmaceutical analysis","volume":"16 4","pages":"101450"},"PeriodicalIF":8.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel bioactive peptides targeting Keap1-Nrf2 interaction for combating UVA-induced skin aging: Computational discovery and experimental validation. 针对Keap1-Nrf2相互作用的新型生物活性肽对抗uva诱导的皮肤衰老：计算发现和实验验证。

IF 8.9

Journal of pharmaceutical analysis Pub Date : 2026-04-01 Epub Date: 2025-09-09 DOI: 10.1016/j.jpha.2025.101446

Haiqiong Guo, Yueting Sun, Wenyu Shi, Rui Huang, Xiaobei Zhi, Qingsong Liu, Ping Zhao, Qingyou Xia

{"title":"Novel bioactive peptides targeting Keap1-Nrf2 interaction for combating UVA-induced skin aging: Computational discovery and experimental validation.","authors":"Haiqiong Guo, Yueting Sun, Wenyu Shi, Rui Huang, Xiaobei Zhi, Qingsong Liu, Ping Zhao, Qingyou Xia","doi":"10.1016/j.jpha.2025.101446","DOIUrl":"10.1016/j.jpha.2025.101446","url":null,"abstract":"Ultraviolet A (UVA)-induced skin aging poses a significant threat to skin health and aesthetics, yet effective and biosafe therapeutic interventions remain scarce. This study focused on identifying bioactive peptide inhibitors targeting the Kelch-like ECH-associated protein 1 (Keap1)-nuclear factor erythroid 2-related factor 2 (Nrf2) protein-protein interaction (PPI) to counteract UVA-induced skin aging. Using computational virtual screening, we identified two high-affinity, low-toxicity peptides, Seq1 and Seq3, which effectively activated the Nrf2-antioxidant response element (ARE) pathway. This activation led to the upregulation of antioxidant genes and significantly reduced oxidative stress. Additionally, these peptides inhibited the mitogen-activated protein kinase (MAPK) and nuclear factor kappa-B (NF-κB) signaling pathways, thereby reducing inflammation and suppressing the expression of matrix metalloproteinases (MMPs), key contributors to skin aging. In vivo studies demonstrated that Seq1 and Seq3 effectively prevented UVA-induced epidermal thickening, collagen degradation, and the upregulation of pro-inflammatory cytokines in mouse models. Our results underscore the therapeutic potential of Seq1 and Seq3, particularly Seq3, as novel bioactive peptides targeting the Keap1-Nrf2 PPI for combating UVA-induced skin aging, offering promising avenues for skincare and healthcare applications.","PeriodicalId":94338,"journal":{"name":"Journal of pharmaceutical analysis","volume":"16 4","pages":"101446"},"PeriodicalIF":8.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative perspective on the geographical origin and quality of Peucedanum praeruptorum Dunn through the integration of inorganic and organic substance profiles. 从无机和有机物质剖面整合的角度创新地看待前南的地理来源和品质。

IF 8.9

Journal of pharmaceutical analysis Pub Date : 2026-04-01 Epub Date: 2025-07-11 DOI: 10.1016/j.jpha.2025.101405

Yaolei Li, Hao Wu, Jing Fan, Jinjian Huang, Hongyu Jin, Feng Wei

{"title":"Innovative perspective on the geographical origin and quality of Peucedanum praeruptorum Dunn through the integration of inorganic and organic substance profiles.","authors":"Yaolei Li, Hao Wu, Jing Fan, Jinjian Huang, Hongyu Jin, Feng Wei","doi":"10.1016/j.jpha.2025.101405","DOIUrl":"10.1016/j.jpha.2025.101405","url":null,"abstract":"The clinical efficacy of traditional Chinese medicines (TCMs) is closely linked to their genuine quality. Identifying the geographical origin and genuine characteristics of medicinal materials is pivotal for enhancing quality control and efficacy. Qianhu (Peucedanum praeruptorum Dunn), a commonly used TCM, still lacks a clear understanding of the specific connection between its quality and geographical origin. To bridge this gap, we innovatively propose a new recognition model that integrates inorganic and organic substances, using Qianhu as the model TCM. By incorporating the concepts of metallomics and metabolomics, we merge elemental fingerprint profiles with chemical component contour maps to pinpoint its geographical origin. The research findings suggested that, compared to chemometrics, machine learning with data oversampling could precisely identify Qianhu from areas like Anhui, Zhejiang, Guizhou, and Chongqing of China, especially distinguishing genuine ones. Upon this groundwork, we further introduced an innovative ensemble model that deeply integrated the optimal models for elements and chemical components, thereby substantially enhancing classification accuracy. Additionally, through variable importance analysis, we provided professional and in-depth interpretations for the elements and chemical components within the model. In summary, this study, for the first time, revealed the scientific basis of Qianhu's producing area and genuine quality through machine learning, integrating inorganic and organic factors. It provides a solid foundation for scientific and reasonable quality control and clinical application of TCMs.","PeriodicalId":94338,"journal":{"name":"Journal of pharmaceutical analysis","volume":"16 4","pages":"101405"},"PeriodicalIF":8.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122225/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0