Endocrine, metabolic & immune disorders drug targets最新文献

{"title":"Evaluating the Relationship between Cathepsins and Papillary Thyroid Carcinoma: A Mendelian Randomization Study.","authors":"Liu Muge, Xiao Xiongsheng, Jin Ling, Li Siyi, Zheng Changwei, Chen Zhengde, Chen Zhuoting, Zhi Zhang","doi":"10.2174/0118715303305715240912172648","DOIUrl":"https://doi.org/10.2174/0118715303305715240912172648","url":null,"abstract":"<p><strong>Background: </strong>Papillary Thyroid Carcinoma (PTC) is the most common thyroid cancer, with an etiology and progression that are not fully understood. Research suggests a link between cathepsins and PTC, but the causal nature of this link is unclear. This study uses Mendelian Randomization (MR) to investigate if cathepsins causally influence PTC risk.</p><p><strong>Methods: </strong>We applied univariable and multivariable MR analyses using genetic variants as proxies for cathepsin levels. Genetic data for cathepsins were sourced from the INTERVAL study, while PTC data came from the Finnish Genome-Wide Association Study database. Our analysis employed several MR methods, including the Inverse Variance Weighted (IVW) approach, MR-Egger, and the Weighted Median method, to provide comprehensive insights and address possible pleiotropy.</p><p><strong>Results: </strong>MR findings suggest a significant causal association between higher cathepsin levels and increased PTC risk. Notably, genetic variants indicating higher cathepsin Z expression were positively causal associated with PTC risk (OR:1.1190, 95% CI: 1.0029-1.2486), multivariable analysis confirmed significant carcinogenesis role of cathepsin Z in PTC (OR: 1.1593, 95% CI: 1.0137-1.3258), with results consistent across various tests, indicating a robust relationship.</p><p><strong>Conclusion: </strong>This study established a causal link between cathepsin levels and PTC risk, emphasizing the roles of cathepsin Z in its progression. These insights could lead to new therapeutic strategies targeting these enzymes. Further research is necessary to understand the underlying biological mechanisms and their clinical implications.</p>","PeriodicalId":94316,"journal":{"name":"Endocrine, metabolic & immune disorders drug targets","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142961036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut Microbiota and Diabetes: Pioneering New Treatment Frontiers.","authors":"Rupendra Shakya, Ponnurengam Malliappan Sivakumar, Pranav Kumar Prabhakar","doi":"10.2174/0118715303342579241119155225","DOIUrl":"https://doi.org/10.2174/0118715303342579241119155225","url":null,"abstract":"<p><p>Diabetes Mellitus (DM) is a complex metabolic disorder characterized by chronic hyperglycemia and poses significant global health challenges. Conventional treatments, such as insulin therapy and lifestyle modifications, have shown limited efficacy in addressing the multifactorial nature of DM. Emerging evidence suggests that gut microbiota, a diverse community of microorganisms critical for metabolism and immune function, plays a pivotal role in metabolic health. Dysbiosis, an imbalance in gut microbiota composition, has been linked to insulin resistance, obesity, and DM. Gut microbiota influences glucose metabolism through mechanisms, including short-chain fatty acid production, gut permeability regulation, and immune system interactions, indicating a bidirectional relationship between microbial health and metabolism. Clinical and experimental studies demonstrate that modulating gut microbiota through dietary interventions (prebiotics, probiotics, synbiotics) improves glycemic control and insulin sensitivity in DM patients. Fecal Microbiota Transplantation (FMT) has also shown promise in restoring healthy gut microbiota and alleviating DM-related metabolic disturbances. However, challenges remain, including the need for personalized treatments due to individual microbiota variability and the unknown long-term effects of these interventions. Future research should focus on elucidating the mechanisms by which gut microbiota influences metabolism and refining personalized approaches to enhance DM management.</p>","PeriodicalId":94316,"journal":{"name":"Endocrine, metabolic & immune disorders drug targets","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142961048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Programmed Cell Death-related Biomarkers for the Potential Diagnosis and Treatment of Osteoporosis.","authors":"Yancheng Huo, Meng Guo, Yihan Li, Xingchen Yao, Qingxian Tian, Tie Liu","doi":"10.2174/0118715303326112241021061805","DOIUrl":"https://doi.org/10.2174/0118715303326112241021061805","url":null,"abstract":"<p><strong>Background: </strong>Osteoporosis (OP) is a skeletal condition characterized by increased susceptibility to fractures. Programmed cell death (PCD) is the orderly process of cells ending their own life that has not been thoroughly explored in relation to OP.</p><p><strong>Objective: </strong>This study is to investigate PCD-related genes in OP, shedding light on potential mechanisms underlying the disease.</p><p><strong>Methods: </strong>Public datasets (GSE56814 and GSE56815) were analyzed to identify differentially expressed genes (DEGs). We employed the least absolute shrinkage and selection operator (LASSO), Boruta, and random forest (RF) algorithms to pinpoint hub PCD-related genes in OP and construct a predictive nomogram model. The performance of the model was validated through ROC curve analysis, calibration curves, and decision curve analysis. Additionally, transcription factor (TF) interaction analysis and functional enrichment analysis were conducted to explore the regulatory networks and biological pathways involved.</p><p><strong>Results: </strong>We identified 161 DEGs, with 30 prominently associated with PCD. Five hub genes, PDPK1, MAP1LC3B, ZFP36, DRAM1, and MPO, were highlighted as particularly significant. A predictive nomogram integrating these genes demonstrated high accuracy (AUC) in forecasting OP risk, with an AUC of 0.911 in the GSE56815 dataset. The validation confirmed the gene model efficacy in differentiating OP risk and clinical applicability. The subsequent TF-gene interaction analyses revealed that these hub genes are regulated by multiple TFs, indicating their central role in OP pathology. Functional enrichment analysis of the hub genes indicated significant involvement in apoptosis, autophagy, and immune response pathways.</p><p><strong>Conclusion: </strong>This study identified PDPK1, MAP1LC3B, ZFP36, DRAM1, and MPO as potential biomarkers and proposes a nomogram based on hub genes for predicting osteoporosis risk.</p>","PeriodicalId":94316,"journal":{"name":"Endocrine, metabolic & immune disorders drug targets","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142961058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Biomarkers Related to Liquid-Liquid Phase Separation for Ulcerative Colitis Based on Single-Cell and Bulk RNA Transcriptome Sequencing Data.","authors":"Jicheng Lu, Xu Lu, Bin Chen","doi":"10.2174/0118715303355042241208171133","DOIUrl":"https://doi.org/10.2174/0118715303355042241208171133","url":null,"abstract":"<p><strong>Background: </strong>Liquid-Liquid Phase Separation (LLPS) is a process involved in the formation of established organelles and various condensates that lack membranes; however, the relationship between LLPS and Ulcerative Colitis (UC) remains unclear.</p><p><strong>Aims: </strong>This study aimed to comprehensively clarify the correlation between ulcerative colitis (UC) and liquid-liquid phase separation (LLPS).</p><p><strong>Objectives: </strong>In this study, bioinformatics analyses and public databases were applied to screen and validate key genes associated with LLPS in UC. Furthermore, the roles of these key genes in UC were comprehensively analyzed.</p><p><strong>Methods: </strong>Based on the single-cell transcriptomic data of UC obtained from the Gene Expression Omnibus (GEO) database, differences between patients with UC and their controls were compared using the limma package. The single-cell data were then filtered and normalized by the 'Seurat' package and subjected to dimension reduction by the Uniform Manifold Approximation and Projection (UMAP) algorithm. The LLPS-related genes (LLPSRGs) were searched on the Dr- LLPS website to obtain cross-correlated genes, which were scored using the ssGSEA algorithm. Next, functional enrichment, interaction network, immune landscape, and diagnostic and drug prediction of the LLPSRGs were comprehensively explored. Finally, the results were validated using external datasets and quantitative real-time PCR (qRT-PCR).</p><p><strong>Results: </strong>A total of eight cell types in UC were classified, namely, fibroblasts, macrophages, endothelial cells, neutrophils, NK cells, B cells, epithelial cells, and T cells. The intersection between differently expressed genes (DEGs) among the eight cell types identified 44 key genes, which were predominantly enriched in immune- and infection-related pathways. According to receiver operating characteristic (ROC) curves, PLA2G2A, GZMK, CD69, HSP90B1, and S100A11 reached an AUC value of 0.94, 0.95, 0.86, 0.89, and 0.93, respectively. Drug prediction revealed that decitabine, tetrachlorodibenzodioxin, tetradecanoylphorbol acetate, thapsigargin, and cisplatin were the potential small molecular compounds for PLA2G2A, GZMK, CD69, HSP90B1, and S100A11. Immune cell infiltration analysis demonstrated that the infiltration of CD4 memory T cell activation, macrophage M1, T macrophage M0, neutrophils, and mast cell activation was higher in the UC group than in the normal group.</p><p><strong>Conclusion: </strong>The LLPSRGs play crucial roles in UC and can be used as prognostic and diagnostic markers for UC. The current findings contribute to the management of UC.</p>","PeriodicalId":94316,"journal":{"name":"Endocrine, metabolic & immune disorders drug targets","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142961056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interlinking the Cross Talk on Branched Chain Amino Acids, Water Soluble Vitamins and Adipokines in the Type 2 Diabetes Mellitus Etiology.","authors":"Shruthi Rai P, Yashodhar P Bhandary, Shivarajashankara Ym, Akarsha B, Roopa Bhandary, Prajna Rh, Namrata Kg, Savin Cg, Priya Alva, P Katyayani, Sukanya Shetty, Sudhakar Tj","doi":"10.2174/0118715303305579241014112730","DOIUrl":"https://doi.org/10.2174/0118715303305579241014112730","url":null,"abstract":"<p><p>Type 2 Diabetes Mellitus (T2DM) is an etiologically diverse metabolic dysfunction that, if untreated, leads to chronic hyperglycemia. Understanding the etiology of T2DM is critical, as it represents one of the most formidable medical challenges of the twenty-first century. Traditionally, insulin resistance has been recognized as the primary risk factor and a well-known consequence of type 2 diabetes. Emerging evidence suggests that branched-chain amino acids (BCAAs), adipokines, and deficiencies in water-soluble vitamins, such as thiamine and pyridoxine, play significant roles in the development of insulin resistance, a key feature of T2DM. These factors are interconnected through the AMP-activated protein kinase (AMPK) pathway, which regulates various metabolic processes, including glucose transport, lipid synthesis, and inflammatory responses. Dysregulation of AMPK is linked to insulin resistance and metabolic syndrome-related illnesses. Understanding the interplay between BCAAs, adipokines, vitamins, and AMPK may offer new therapeutic targets for the prevention and treatment of diabetes mellitus.</p>","PeriodicalId":94316,"journal":{"name":"Endocrine, metabolic & immune disorders drug targets","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142961060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond the Surface: Tracing the Evolution of Inflammatory Mechanism in Depression through Bibliometric Analysis.","authors":"Zhang-Yang Xu, Ting Zhang, Hong Gong, Hong Zheng, Mei-Shan Liu, Bing-Hang Guo, Yun-Xia Wang, Wen-Jun Su","doi":"10.2174/0118715303325541241024060229","DOIUrl":"https://doi.org/10.2174/0118715303325541241024060229","url":null,"abstract":"<p><strong>Background: </strong>Depression is a common mental illness that has become a major economic burden worldwide. Recently, increasing evidence has highlighted the inflammatory mechanism of depression. In order to understand the research status of this field, this study used the bibliometric analysis method to overview the research content and progress, as well as analyze the development trend and limitations.</p><p><strong>Methods: </strong>In this study, articles and reviews were included in the specific search strategy. The matched papers were exported from the Web of Science database. CiteSpace 6.3 R1 and Bibliometrix (R package) were utilized to generate bibliometric and knowledge maps.</p><p><strong>Results: </strong>A total of 25,063 articles were included in this study. The number of publications in this field has gradually increased, especially in recent years. These papers come from 156 countries, led by the United States and China mainland. The leading research institution is the University of Toronto (Canada). Brain Behavior and Immunity is the journal with the most publications and the most frequently co-cited journals. Among 91,100 authors, Maes M has the most publications and co-citations. According to the keywords burst and co-cited reference analysis, the hotspots in the field in recent years include kynurenine, c-reactive protein, neuroinflammation, and gut microbiota.</p><p><strong>Conclusion: </strong>Although abundant academic achievements have been made on the inflammatory mechanism of depression, there is still a long way to go before these research results can be applied to clinical practice. Strengthening international academic exchanges and cooperation may promote breakthroughs in this field.</p>","PeriodicalId":94316,"journal":{"name":"Endocrine, metabolic & immune disorders drug targets","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142960986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neonatal Pain Management: Is There An Endocrinal Response?","authors":"Mohamed Shawky Elfarargy, Ahmad Roshdy Ahmad, Dalia Hamdy Elbadry","doi":"10.2174/0118715303325556241127080237","DOIUrl":"https://doi.org/10.2174/0118715303325556241127080237","url":null,"abstract":"<p><p>Neonates exhibit pain responses characterized by various endocrinal changes, including alterations in cortisone and oxytocin serum levels, as well as physiological and emotional reactions. The administration of neonatal pain management leads to the normalization of endocrine hormones, including cortisone and oxytocin, which are affected by the presence of neonatal pain. Diagnosing neonatal pain is complex; however, effective management is essential. An adequate balance should be established between the analgesics used for pain management and their associated side effects. Uncontrolled neonatal pain is correlated with delayed development with increased neurologic insult. This review aims to examine the significance of neonatal pain, along with its clinical and physical manifestations. It also explores strategies for managing neonatal pain, encompassing both pharmacological and non-pharmacological methods, along with the particular medications utilized in pharmacological interventions. This discussion includes various non-pharmacological methods for managing neonatal pain. Additionally, this review examines methods for pain assessment. The aim is to highlight the significance of pain in this vulnerable population and to promote the implementation of diverse management strategies for neonatal pain to prevent serious yet avoidable, adverse effects in neonates.</p>","PeriodicalId":94316,"journal":{"name":"Endocrine, metabolic & immune disorders drug targets","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142961014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing Care for Primary Glomerulonephritis: The Role of Thyroid Evaluation.","authors":"Ahmet Numan Demir, Cebrail Karaca, Zehra Kara, Cem Sulu, Serdar Sahin, Emre Durcan, Mevlut Tamer Dincer, Sukran Erdem Nurcan, Ozge Sonmez, Hande Mefkure Ozkaya, Nurhan Seyahi, Mustafa Sait Gonen","doi":"10.2174/0118715303328436241121114054","DOIUrl":"https://doi.org/10.2174/0118715303328436241121114054","url":null,"abstract":"<p><strong>Background: </strong>The coexistence of primary glomerulonephritis and autoimmune thyroid disease has not been investigated.</p><p><strong>Objective: </strong>This study aimed to assess thyroid morphology using sonography, determine the prevalence of autoimmune thyroid disorders, and evaluate thyroid function status in patients diagnosed with primary glomerulonephritis.</p><p><strong>Materials and methods: </strong>This single-center cross-sectional and observational study included 58 consecutive patients with primary glomerulonephritis and 58 healthy controls (HC). All participants underwent thyroid examination through laboratory tests and thyroid ultrasonography. The findings were subsequently compared between the two groups.</p><p><strong>Results: </strong>Among the patients, 17.2% (n = 10) exhibited subclinical hypothyroidism, while 8.6% (n = 5) had overt hypothyroidism. None of the HCs showed overt hypothyroidism, whereas 3.4% (n = 2) of them exhibited subclinical hypothyroidism. Patients displayed significantly lower free triiodothyronine (FT3) levels than HCs (p < 0.001). A linear correlation was observed between patients' thyroid-stimulating hormone (TSH) levels and the degree of proteinuria (p = 0.044). Furthermore, thyroid volume (p < 0.001), hypoechogenicity (p < 0.001), heterogeneous structure (p < 0.001), pseudonodular hypoechoic infiltration (p = 0.05), and the presence of nodules (p < 0.001) were notably higher in patients compared to HCs.</p><p><strong>Conclusion: </strong>The prevalence of both overt and subclinical hypothyroidism, along with the frequency of nodular goiter, was found to be elevated in patients with primary glomerulonephritis. Considering that primary glomerulonephritis predominantly afflicts young individuals, and these patients bear the lifelong burden of chronic kidney disease, we underscore the significance of routine thyroid function tests and sonographic examinations for the early detection of thyroid disorders.</p>","PeriodicalId":94316,"journal":{"name":"Endocrine, metabolic & immune disorders drug targets","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142961015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Potential Systemic Anti-Inflammatory Effect of Turmeric Dried Extract.","authors":"Concetta Montanino, Federica Farinella, Bruna De Felice, Andrea Del Buono, Armando D'Orta","doi":"10.2174/0118715303329562241116045410","DOIUrl":"https://doi.org/10.2174/0118715303329562241116045410","url":null,"abstract":"<p><strong>Background: </strong>Curcumin is a polyphenolic compound derived from the food spice turmeric that has received interest from the medical and scientific world for its role in the management of several conditions. Clinical studies, in humans, have shown that ingested Curcumin is safe even at high doses (12 g/day), but it has poor bioavailability primarily due to poor absorption and rapid metabolism and elimination. Several strategies have been implemented to improve the bioavailability of Curcumin, for example, the combination of piperine in a complex with Curcumin, or the usage of formulations with phospholipid or liposomal complexes.</p><p><strong>Objective: </strong>The present work aims to explore and compare the systemic anti-inflammatory effects of two different types of Curcumin: a traditional fat-soluble formulation (95% Curcumin) and an innovative standardized reconstituted water-soluble one (Curcuin), made in micelles in aqueous solution.</p><p><strong>Methods: </strong>Research was conducted on 30 patients, 15 patients were treated with turmeric (Curcuma longa L., rhizome) dried extract titled 95% Curcumin (Curcumin 425mg/day) conjugated with piperine, and 15 patients were treated with Curcumin (turmeric 286 mg dried extract titled 35%; Curcuminoids 100 mg/day, standardized water-soluble) made in micelles in highly absorbed aqueous solution. We considered the quantitative variations of laboratory parameters: Erythrocyte Sedimentation Rate (ESR), C-reactive protein (CRP), Ferritin (24 to 336 ng/mL for adult males), and cholesterol LDL.</p><p><strong>Results and discussion: </strong>Patients treated with dried extract titled 95% Curcumin, for 90 days, show a lower value of ESR, CRP, Ferritin, and LDL cholesterol compared with the same laboratory parameters before the introduction of Curcumin into the diet. Also, patients treated with Curcuin report a lower value of ESR, CRP, Ferritin, and LDL cholesterol after the introduction of turmeric dried extract in the diet, but with a major significance compared with those obtained with 95% Curcumin conjugated with piperine.</p><p><strong>Conclusion: </strong>As we had hypothesized, both turmeric-derived extracts have successfully reduced ESR, CRP, Ferritin, and cholesterol LDL values, exerting an anti-inflammatory action and anti-cholesterolemic action. These results suggest a possible use of Curcumin and in particular Curcuin as a coadjuvant for the treatment of inflammatory disease and to decrease cholesterol levels. However, additional investigation is needed to resolve doubts regarding Curcumin dosage form, dose, and medication frequency.</p>","PeriodicalId":94316,"journal":{"name":"Endocrine, metabolic & immune disorders drug targets","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142961072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effects of Hydrogen Treatment Against High Glucose-Induced Oxidative Stress and Apoptosis via Inhibition of the AGEs/RAGE/NF-κB Signaling Pathway in Skin Cells.","authors":"Pan Yu, Nan Hong, Qiong Wu, Zhipeng Zhao","doi":"10.2174/0118715303369584241231141001","DOIUrl":"https://doi.org/10.2174/0118715303369584241231141001","url":null,"abstract":"<p><strong>Background: </strong>Diabetic wounds are major clinical challenges, often complicated by oxidative stress and free radical generation. Hydrogen (H2), a selective antioxidant, offers potential as a therapeutic agent for chronic diabetic wounds. However, its precise mechanisms remain underexplored.</p><p><strong>Objective: </strong>This study aimed to investigate the protective effects of H2 on high glucose-induced oxidative damage and apoptosis in human skin cells.</p><p><strong>Methods: </strong>HaCaT keratinocytes and HSF fibroblasts were treated with high glucose or AGEs. Cell viability, oxidative stress markers, inflammatory cytokines, and apoptosis were analyzed. AGEs/RAGE/NF-κB signaling was evaluated via Western blot.</p><p><strong>Results: </strong>H2 treatment significantly reduced ROS, MDA, IL-1β, and TNF-α levels, while enhancing SOD and GSH activity. It also inhibited AGEs/RAGE/NF-κB signaling and apoptosis.</p><p><strong>Conclusion: </strong>Hydrogen therapy protects against oxidative stress and inflammation induced by high glucose or AGEs, offering potential as an adjunctive treatment for diabetic wound healing.</p>","PeriodicalId":94316,"journal":{"name":"Endocrine, metabolic & immune disorders drug targets","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142960963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}