Current research in parasitology & vector-borne diseases最新文献

{"title":"American mitogenome reference for the tropical brown dog tick, Rhipicephalus linnaei (Audouin, 1826)","authors":"Consuelo Almazán ,&nbsp;Lorena Torres Rodríguez ,&nbsp;Abdullah D. Alanazi ,&nbsp;Jan Šlapeta","doi":"10.1016/j.crpvbd.2024.100194","DOIUrl":"https://doi.org/10.1016/j.crpvbd.2024.100194","url":null,"abstract":"<div><p>The brown dog tick, <em>Rhipicephalus linnaei</em> (Audouin, 1826), is distributed across the American continent and is formerly known as the “tropical lineage”. It belongs to the <em>Rhipicephalus sanguineus</em> (Latreille, 1806) species complex, referred to as <em>R. sanguineus</em> (<em>sensu lato</em>). Mitochondrial genome sequences are frequently used for the identification and represent reference material for field studies. In the present study, the entire mitochondrial genomes of <em>R. linnaei</em> (∼15 kb) collected from dogs in Mexico were sequenced and compared with available mitogenomes of <em>R. sanguineus</em> (<em>s.l</em>.). The mitochondrial genome is ∼90% identical to the reference genome of <em>R. sanguineus</em> (<em>sensu stricto</em>, former “temperate lineage”) and &gt; 99% identical to <em>R. linnaei</em> mitogenome derived from the neotype. Two additional mitogenomes were obtained and described as <em>R. linnaei</em> and <em>R. turanicus</em> from dogs in Saudi Arabia. The present study delivers a molecular reference for <em>R. linnaei</em> from America and complements <em>R. linnaei</em> mitogenomes from Africa, Asia and Australia. We propose to consider the complete mitogenome, as the reference for American <em>R. linnaei</em>, even when partial mitochondrial <em>cox</em>1, <em>12S</em> rRNA or <em>16S</em> rRNA genes are characterised.</p></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667114X24000256/pdfft?md5=72c8cf2360604ef2777b47fb388b7cff&pid=1-s2.0-S2667114X24000256-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141484203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Older urban rats are infected with the zoonotic nematode Angiostrongylus cantonensis","authors":"Phoebe Rivory ,&nbsp;Miguel Bedoya-Pérez ,&nbsp;Michael P. Ward ,&nbsp;Jan Šlapeta","doi":"10.1016/j.crpvbd.2024.100179","DOIUrl":"10.1016/j.crpvbd.2024.100179","url":null,"abstract":"<div><p>Rats, being synanthropic, are hosts to agents of zoonotic diseases that pose a threat to human and domestic animal health. The nematode parasite <em>Angiostrongylus cantonensis</em>, commonly known as the rat lungworm, is no exception; it can cause potentially fatal neural disease in humans, dogs and other species. The distribution of <em>A. cantonensis</em> (haplotypes SYD.1 and Ac13) and its close relative, <em>Angiostrongylus mackerrasae</em> is not well understood in Australia. We investigated the prevalence of <em>Angiostrongylus</em> in rats in Sydney, Australia, primarily <em>via</em> faecal qPCR, and identified the species and haplotypes using partial <em>cox</em>1 sequencing. We found a moderate prevalence of infection (29%; 95% CI: 16.1–46.6%) in black (<em>Rattus rattus</em>) and brown (<em>Rattus norvegicus</em>) rats around public parks and residential areas. This study demonstrates that Sydney’s urban rat population is a reservoir for <em>A. cantonensis</em>. Modelling infection status as a function of rat species, sex, tibia length (as a proxy for age), and health index (a measure of weight by size) revealed that older rats are statistically more likely to be infected (<em>χ</em>²₁ = 5.331, <em>P</em> = 0.021). We observed a dominant presence of the <em>A. cantonensis</em> SYD.1 haplotype, for which the implications are not yet known. No <em>A. mackerassae</em> was detected, leading us to suspect it may have a more restricted host- and geographical range. Overall, this study illustrates the presence and potential risk of <em>A. cantonensis</em> infection in Sydney. Public education regarding transmission routes and preventative measures is crucial to safeguard human and animal health.</p></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667114X24000104/pdfft?md5=911084a9ef1b8fc2258110b24fbe4479&pid=1-s2.0-S2667114X24000104-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141144716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A brief review and guidance on the spatiotemporal sampling designs for disease vector surveillance","authors":"Abdollah Jalilian ,&nbsp;Jorge Mateu ,&nbsp;Luigi Sedda","doi":"10.1016/j.crpvbd.2024.100208","DOIUrl":"10.1016/j.crpvbd.2024.100208","url":null,"abstract":"<div><p>Obtaining a representative sample of disease vectors (mosquitoes, flies, ticks, etc.) is essential for researchers to draw meaningful conclusions about the entire vector population in a target study area and during a specific study period. To achieve this, a carefully chosen surveillance design is required to ensure that the sample captures essential spatial and temporal variations in the target vector population(s) and/or that the study results can be generalized to the entire population. Designed-based and model-based spatiotemporal sampling (or in our context surveillance) designs can be used to maximize information gain within given resource constraints. In this paper, we aim to offer a concise overview of common spatiotemporal field sampling designs, their advantages and disadvantages and their practical applications in the context of surveillance and management of vector-borne diseases. At the end of the article, we offer guidance to help vector-borne disease surveillance planners design effective spatiotemporal surveillance interventions.</p></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667114X24000396/pdfft?md5=47755f5661f99f9f08a5637ef8ffc394&pid=1-s2.0-S2667114X24000396-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142097806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Update on infections with Thelazia callipaeda in European wildlife and a report in a red fox, Vulpes vulpes, in Portugal","authors":"Ana Filipa Lopes ,&nbsp;Mariana Ribeiro Ferreira ,&nbsp;Beatriz do Vale ,&nbsp;Marlene Santos ,&nbsp;Inês Silveira ,&nbsp;Sofia Claudino ,&nbsp;Manuel Martins ,&nbsp;Telma Brida ,&nbsp;Luís Figueira ,&nbsp;Luís Cardoso ,&nbsp;Ana Patrícia Lopes ,&nbsp;Ana Cláudia Coelho ,&nbsp;Manuela Matos ,&nbsp;Ana Cristina Matos","doi":"10.1016/j.crpvbd.2024.100211","DOIUrl":"10.1016/j.crpvbd.2024.100211","url":null,"abstract":"<div><p><em>Thelazia callipaeda</em>, also known as the “oriental eye worm”, is a zoonotic parasitic nematode with a wide range of hosts, particularly wild and domestic carnivores, but also lagomorphs and humans. Currently, ocular thelaziosis presents an expanding distribution range throughout Europe, including Portugal. This study provides an update on <em>T</em>. <em>callipaeda</em> infection reports (30 studies) in European wildlife comprising 54 host-locality records in 10 host species from nine European countries. The prevalence of <em>T</em>. <em>callipaeda</em> varied widely, with ranges from around 1% in red foxes and European hares to almost 50% in red foxes. The lowest mean intensity was 2.7 nematodes/host in European wildcats and the highest was 38.0 nematodes/host in wolves. In addition, a massive infection with <em>T. callipaeda</em> in a juvenile male red fox from eastern-central Portugal is also described, representing the southernmost report in a wild animal in this country. A total of 188 nematodes (139 females and 49 males) were collected from both eyes and were submitted to morphological and molecular characterization. Collected nematodes were morphologically identified as <em>T</em>. <em>callipaeda</em>. Given the endemicity of <em>T</em>. <em>callipaeda</em> in eastern-central Portugal, surveillance system should be implemented to monitor its presence among wild and domestic animals.</p></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667114X24000426/pdfft?md5=9801eaa30055e263a86a3129adb9727b&pid=1-s2.0-S2667114X24000426-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142084150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suitability of red fox (Vulpes vulpes) and golden jackal (Canis aureus) as hosts of Echinococcus multilocularis based on egg production characteristics and literature data on the intestinal ecosystems of carnivores","authors":"Sibusiso Moloi,&nbsp;Tibor Halász,&nbsp;Ágnes Csivincsik,&nbsp;Gábor Nagy","doi":"10.1016/j.crpvbd.2024.100225","DOIUrl":"10.1016/j.crpvbd.2024.100225","url":null,"abstract":"<div><div><em>Echinococcus multilocularis</em> is the most important food-borne parasite in Europe. Its natural definitive host is the red fox (<em>Vulpes vulpes</em>) while other canid species play a secondary role in the maintenance of its endemics. However, recent studies call attention to the potential of golden jackal (<em>Canis aureus</em>) as a suitable definitive host for <em>E. multilocularis</em>. Our study aimed to evaluate the quantitative and qualitative egg production traits of adult <em>E. multilocularis</em> in different hosts as an indicator of reproductive success. Investigation of 111 and 82 parasites from 33 red foxes and 29 golden jackals, respectively, we ascertained that the proportion of worms with mature eggs was significantly lower in golden jackals than in red foxes. Those worms, which produced mature eggs in golden jackal hosts, originated from less crowded infrapopulations than their fox-originated counterparts. Other characteristics of the parasite’s reproductive ability, such as the proportion of fertile worms, and mean egg production were similar in the two hosts. Comparing these findings to evolutionary data on different canid taxa, we hypothesised that the mutual presence of red fox and a differently evolved host of <em>E. multilocularis</em> might contribute to the formation of stable parasite circulation in these multi-host systems.</div></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142573361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Helminth fauna of the black goby Gobius niger L. (Gobiiformes: Gobiidae) from the Finnish Archipelago, Baltic Sea: Molecular and morphological data","authors":"Inga Martinek ,&nbsp;Jesús S. Hernández-Orts","doi":"10.1016/j.crpvbd.2023.100169","DOIUrl":"10.1016/j.crpvbd.2023.100169","url":null,"abstract":"<div><p>Black gobies (<em>Gobius niger</em>) from the Finnish Archipelago, Baltic Sea, were screened for helminth infections in summer 2020. Helminths were identified morphologically and/or molecularly. Altogether 26 novel sequences were generated and analysed using maximum likelihood estimation. Morphological and phylogenetic analyses based on mitochondrial genes revealed the presence of 8 species belonging to the Digenea (<em>Diplostomum mergi</em> Lineage 3), Cestoda (<em>Bothriocephalus scorpii</em>), Nematoda (<em>Contracaecum rudolphii</em> A, <em>Cucullanus</em> sp. and <em>Hysterothylacium aduncum</em>), and Acanthocephala (a putative new species of <em>Corynosoma</em>, <em>Corynosoma semerme</em> and <em>Neoechinorhynchus</em> sp.). Phylogenetic and comparative sequence analyses revealed that the putative new acanthocephalan species is closely related to <em>C. neostrumosum</em> described from the Caspian seal, <em>Pusa caspica</em>, in the Caspian Sea. The black goby represents a new host record for four parasite species (<em>Diplostomum mergi</em> Lineage 3, <em>Contracaecum rudolphii</em> A, <em>Corynosoma semerme</em> and <em>Corynosoma</em> sp.). The Finnish Archipelago is a novel locality record for three species (<em>Corynosoma</em> sp., <em>Diplostomum mergi</em> Lineage 3 and <em>Bothriocephalus scorpii</em>).</p></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667114X23000572/pdfft?md5=75f3fe78aac30287f0456f501155bd59&pid=1-s2.0-S2667114X23000572-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139187758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case of abnormal swimming patterns in juvenile Oblada melanura naturally infected with Philometra obladae (Nematoda: Philometridae) in the Tyrrhenian Sea off Sicily, Italy","authors":"Giovanni De Benedetto,&nbsp;Gabriella Gaglio","doi":"10.1016/j.crpvbd.2023.100167","DOIUrl":"10.1016/j.crpvbd.2023.100167","url":null,"abstract":"<div><p>The saddled seabream, <em>Oblada melanura</em> (L.), is a common seawater species present in the Mediterranean. Between July and August 2023, during diving activities along the Sicilian coast of Italy, we recorded with a digital camera several specimens of <em>O. melanura</em> showing an abnormal pattern of swimming in the water column. The unusual swimming behaviour was characterized by fast, uncoordinated directional changes and isolation from the remaining fishes in the shoal. Four dead fish were found and collected for necropsy and parasitological evaluation/examination. Upon gross examination, all fish showed an evident coelomic distension; the coelomic cavity of two fish was filled with nematodes that dislocated the coelomic organs, and the other two had degraded nematodes and a conspicuous quantity of fluid in the coelomic cavity. All collected parasites were identified as <em>Philometra obladae</em> (Nematoda: Philometridae) according to morphological criteria. Here, we describe the unusual swimming behaviour of <em>O. melanura</em> naturally infected with <em>Ph. obladae</em> and the results of the examination of dead fish infected with this parasite. Future studies are needed to better evaluate and describe the dynamics and the epidemiology of <em>Ph. obladae</em> infection in wild <em>O. melanura</em>.</p></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667114X23000559/pdfft?md5=d6d5e0860b4b9bd591cca2c1e4fe0c4f&pid=1-s2.0-S2667114X23000559-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139190657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Germany-wide citizen science study reveals spread of Babesia canis-infected Dermacentor reticulatus ticks by dogs travelling within the country","authors":"Andrea Springer ,&nbsp;Alexander Lindau ,&nbsp;Julia Probst ,&nbsp;Katrin Fachet ,&nbsp;Ingo Schäfer ,&nbsp;Gerhard Dobler ,&nbsp;Ute Mackenstedt ,&nbsp;Christina Strube","doi":"10.1016/j.crpvbd.2024.100187","DOIUrl":"10.1016/j.crpvbd.2024.100187","url":null,"abstract":"<div><p>The ornate dog tick <em>Dermacentor reticulatus</em>, vector of <em>Babesia canis</em>, has shown a considerable range expansion in several European countries. Previously, only few areas in Germany were recognised as endemic for <em>B. canis</em>, but a marked increase in autochthonous canine babesiosis cases and spread to new areas has been noted recently. To better assess the current risk for dogs, the present study screened 5913 specimens of <em>D. reticulatus</em> from all over Germany, collected in the frame of a Citizen Science study during 2019–2023. Moreover, 343 <em>Dermacentor marginatus</em> ticks were also included. <em>Babesia</em> detection was achieved by quantitative real-time PCR (qPCR). Positive samples were confirmed by sequencing. Moreover, a MGB-probe-based triplex qPCR was established to detect and distinguish between the canine <em>Babesia</em> spp. relevant in Europe, i.e. <em>B. canis</em>, <em>Babesia vogeli</em> and <em>Babesia gibsoni</em>. Overall, <em>B. canis</em> DNA was detected in five <em>D. reticulatus</em> specimens (0.08%). Two of the <em>B. canis</em>-positive ticks originated from areas previously known as endemic for canine babesiosis, namely from the area of Freiburg im Breisgau, federal state of Baden-Wuerttemberg, and from the district St. Wendel, federal state of Saarland. Three further <em>B. canis</em>-positive ticks were detected in districts not yet recognised as endemic, one each in the district of Mansfeld-Suedharz, federal state of Saxony-Anhalt, the district of Ravensburg, federal state of Baden-Wuerttemberg and in the city of Fürth, federal state of Bavaria. However, the tick in Fürth was found on a dog who had returned from a trip to the Breisgau region on the previous day, indicating translocation of the specimen out of this well-known endemic focus. The geographical distribution of the positive samples shows that <em>B. canis</em> is currently spreading in Germany, particularly <em>via</em> dogs travelling within the country, increasing the infection risk throughout the country. Important measures to contain a further spread of the pathogen include comprehensive year-round tick prophylaxis with licensed acaricides, not only to protect the individual pet, but also the entire dog population. Moreover, screening of dogs entering Germany from <em>B. canis</em>-endemic countries is required and any treatment should aim at pathogen elimination by use of appropriate imidocarb dosages.</p></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667114X24000189/pdfft?md5=2a95e0eb687f91f1e84f4aea0463183b&pid=1-s2.0-S2667114X24000189-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141409120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clockwork intruders: Do parasites manipulate their hostsʼ circadian rhythms?","authors":"Sebastián Boy-Waxman ,&nbsp;Martin Olivier ,&nbsp;Nicolas Cermakian","doi":"10.1016/j.crpvbd.2024.100171","DOIUrl":"10.1016/j.crpvbd.2024.100171","url":null,"abstract":"<div><p>Most organisms have developed circadian clocks to adapt to 24-hour cycles in the environment. These clocks have become crucial for modulating and synchronizing complex behavioral and biological processes. A number of parasites seem to have evolved to take advantage of their hosts’ circadian rhythms to favor their own infection and survival. Some species, such as <em>Microphallus</em> sp. and <em>Trypanosoma cruzi</em>, can alter the patterns of locomotor behavior of infected intermediate hosts, which can promote transmission to a subsequent primary host. Some fungi of the genera <em>Ophiocordyceps</em> and <em>Entomophthora</em>, as well as hairworms (Nematomorpha), elicit complex behaviors that promote their host’s death at a time and place that optimizes continuation of the parasite’s life-cycle. At least in some cases, a proposed mechanism might involve a change in the expression of clock-controlled genes. Lastly, some disease-causing protozoan parasites of the genera <em>Trypanosoma</em>, <em>Plasmodium</em>, and <em>Leishmania</em> induce changes in the circadian rhythms of their primary hosts upon infection. Some of these changes may be attributed to circadian alterations resulting from the host’s inflammatory response to the infection or other unexplored responses or adaptations to the illness. Thus, a distinction must be made between manipulation of the parasite and response of the host when studying these alterations in the future. Parasitic manipulation of circadian rhythms, which vastly modulates behavior and physiology, is an essential issue that has been relatively understudied. A deeper understanding of this phenomenon could lead to the development of novel therapeutic approaches for the diseases that these parasites convey.</p></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667114X24000025/pdfft?md5=450d349550989e85087d9517d988c999&pid=1-s2.0-S2667114X24000025-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139888302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity of Ehrlichia spp., Anaplasma spp. and Neorickettsia spp. in vampire bats","authors":"Victória Valente Califre de Mello ,&nbsp;Laryssa Borges de Oliveira ,&nbsp;Taciana Fernandes Souza Barbosa Coelho ,&nbsp;Daniel Antonio Braga Lee ,&nbsp;Lorena Freitas das Neves ,&nbsp;Eliz Oliveira Franco ,&nbsp;Anna Claudia Baumel Mongruel ,&nbsp;Rosangela Zacarias Machado ,&nbsp;Marcos Rogério André","doi":"10.1016/j.crpvbd.2024.100182","DOIUrl":"https://doi.org/10.1016/j.crpvbd.2024.100182","url":null,"abstract":"<div><p>Although bats (Mammalia: Chiroptera) act as natural reservoirs for many zoonotic pathogens around the world, few studies have investigated the occurrence of <em>Anaplasmataceae</em> agents in bats, especially vampire bats. The family <em>Anaplasmataceae</em> (order Rickettsiales) encompasses obligate intracellular bacteria of the genera <em>Anaplasma</em>, <em>Ehrlichia</em>, <em>Neorickettsia</em>, <em>Neoehrlichia</em>, <em>Wolbachia</em>, and <em>Allocryptoplasma</em>. The present study aimed to investigate, using molecular techniques, the presence of species of <em>Anaplasma</em>, <em>Ehrlichia</em>, and <em>Neorickettsia</em> in vampire bats sampled in northern Brazil. Between 2017 and 2019, spleen samples were collected from vampire bats belonging to two species, <em>Desmodus rotundus</em> (<em>n =</em> 228) from the states of Pará (<em>n</em> = 207), Amazonas (<em>n</em> = 1), Roraima (<em>n</em> = 18) and Amapá (<em>n</em> = 3), and <em>Diaemus youngii</em> (<em>n</em> = 1) from Pará. Positivity rates of 5.2% (12/229), 3% (7/229), and 10.9% (25/229) were found in PCR assays for <em>Anaplasma</em> spp. (16S rRNA gene), <em>Ehrlichi</em>a spp. (<em>dsb</em> gene) and <em>Neorickettsia</em> spp. (16S rRNA gene), respectively. The present study revealed, for the first time, the occurrence of <em>Anaplasma</em> spp. and different genotypes of <em>Ehrlichia</em> spp. in vampire bats from Brazil. While phylogenetic analyses based on the <em>dsb</em> and <em>ftsZ</em> genes of <em>Ehrlichia</em> and 16S rRNA of <em>Anaplasma</em> spp. revealed phylogenetic proximity of the genotypes detected in vampire bats with <em>Anaplasmataceae</em> agents associated with domestic ruminants, phylogenetic inferences based on the <em>gltA</em> and <em>groEL</em> genes evidenced the occurrence of genotypes apparently exclusive to bats. <em>Neorickettsia</em> sp. phylogenetically associated with <em>N. risticii</em> was also detected in vampire bats sampled in northern Brazil.</p></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667114X2400013X/pdfft?md5=cce51e68f2255593753a2d72e12a0c1c&pid=1-s2.0-S2667114X2400013X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141304004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}