Current research in parasitology & vector-borne diseases最新文献

{"title":"Germany-wide citizen science study reveals spread of Babesia canis-infected Dermacentor reticulatus ticks by dogs travelling within the country","authors":"Andrea Springer ,&nbsp;Alexander Lindau ,&nbsp;Julia Probst ,&nbsp;Katrin Fachet ,&nbsp;Ingo Schäfer ,&nbsp;Gerhard Dobler ,&nbsp;Ute Mackenstedt ,&nbsp;Christina Strube","doi":"10.1016/j.crpvbd.2024.100187","DOIUrl":"10.1016/j.crpvbd.2024.100187","url":null,"abstract":"<div><p>The ornate dog tick <em>Dermacentor reticulatus</em>, vector of <em>Babesia canis</em>, has shown a considerable range expansion in several European countries. Previously, only few areas in Germany were recognised as endemic for <em>B. canis</em>, but a marked increase in autochthonous canine babesiosis cases and spread to new areas has been noted recently. To better assess the current risk for dogs, the present study screened 5913 specimens of <em>D. reticulatus</em> from all over Germany, collected in the frame of a Citizen Science study during 2019–2023. Moreover, 343 <em>Dermacentor marginatus</em> ticks were also included. <em>Babesia</em> detection was achieved by quantitative real-time PCR (qPCR). Positive samples were confirmed by sequencing. Moreover, a MGB-probe-based triplex qPCR was established to detect and distinguish between the canine <em>Babesia</em> spp. relevant in Europe, i.e. <em>B. canis</em>, <em>Babesia vogeli</em> and <em>Babesia gibsoni</em>. Overall, <em>B. canis</em> DNA was detected in five <em>D. reticulatus</em> specimens (0.08%). Two of the <em>B. canis</em>-positive ticks originated from areas previously known as endemic for canine babesiosis, namely from the area of Freiburg im Breisgau, federal state of Baden-Wuerttemberg, and from the district St. Wendel, federal state of Saarland. Three further <em>B. canis</em>-positive ticks were detected in districts not yet recognised as endemic, one each in the district of Mansfeld-Suedharz, federal state of Saxony-Anhalt, the district of Ravensburg, federal state of Baden-Wuerttemberg and in the city of Fürth, federal state of Bavaria. However, the tick in Fürth was found on a dog who had returned from a trip to the Breisgau region on the previous day, indicating translocation of the specimen out of this well-known endemic focus. The geographical distribution of the positive samples shows that <em>B. canis</em> is currently spreading in Germany, particularly <em>via</em> dogs travelling within the country, increasing the infection risk throughout the country. Important measures to contain a further spread of the pathogen include comprehensive year-round tick prophylaxis with licensed acaricides, not only to protect the individual pet, but also the entire dog population. Moreover, screening of dogs entering Germany from <em>B. canis</em>-endemic countries is required and any treatment should aim at pathogen elimination by use of appropriate imidocarb dosages.</p></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":"6 ","pages":"Article 100187"},"PeriodicalIF":1.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667114X24000189/pdfft?md5=2a95e0eb687f91f1e84f4aea0463183b&pid=1-s2.0-S2667114X24000189-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141409120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is it possible to eliminate or eradicate human fish-borne parasitic diseases? A sweet dream or a nightmare?","authors":"Jorge C. Eiras","doi":"10.1016/j.crpvbd.2024.100203","DOIUrl":"10.1016/j.crpvbd.2024.100203","url":null,"abstract":"<div><p>Human fish-borne parasitic diseases may be caused by at least 111 taxa of both freshwater and marine fish parasites. It is estimated that they occur in many hundreds of millions of people all over the world, and many more are at risk, sometimes with serious consequences including the death of the host. Therefore, all efforts must be made to minimize and prevent the infection. In this paper we present an overview detailing the several types of parasites infecting humans, the reasons for the occurrence of the disease, the ways of infection, the preventive measures and difficulties encountered when combating such infections. Finally, we discuss the possibility of eliminating or eradicating fish-borne diseases. It is concluded that elimination is difficult to achieve but it is possible in some places under favourable circumstances, and that eradication will probably never be fully achieved.</p></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":"6 ","pages":"Article 100203"},"PeriodicalIF":1.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667114X24000347/pdfft?md5=b55394f4b93210ada5e1eefeebef771f&pid=1-s2.0-S2667114X24000347-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141839953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the wing cell contour to distinguish between Stomoxys bengalensis and Stomoxys sitiens (Diptera: Muscidae) using outline-based morphometrics","authors":"Thekhawet Weluwanarak ,&nbsp;Tanawat Chaiphongpachara ,&nbsp;Tanasak Changbunjong","doi":"10.1016/j.crpvbd.2024.100204","DOIUrl":"10.1016/j.crpvbd.2024.100204","url":null,"abstract":"<div><p>The blood-sucking flies of the genus <em>Stomoxys</em> Geoffroy, 1762 (Diptera: Muscidae) are significant ectoparasites that can cause irritation and transmit pathogens to both animals and humans. Within the genus <em>Stomoxys</em>, two species, <em>Stomoxys bengalensis</em> and <em>Stomoxys sitiens</em>, have similar morphology and coexist in the same habitat. Accurate species identification of these flies is crucial for understanding disease vectors and implementing effective control measures. In this study, we assessed the effectiveness of outline-based geometric morphometrics (GM) by analyzing the wing cell contour of the first posterior cell (R₅) to distinguish between species and sexes of <em>S. bengalensis</em> and <em>S. sitiens</em>. Our results demonstrate that the outline-based GM method is highly effective in distinguishing between species and sexes of these flies based on contour shape, with accuracy scores ranging from 90.0% to 97.5%. Therefore, outline-based GM emerges as a promising alternative to landmark-based GM or as a supplementary tool in conjunction with traditional morphology-based methods for species identification.</p></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":"6 ","pages":"Article 100204"},"PeriodicalIF":1.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667114X24000359/pdfft?md5=f419397867c4b9f3f049d0ef63233d61&pid=1-s2.0-S2667114X24000359-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141845179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clockwork intruders: Do parasites manipulate their hostsʼ circadian rhythms?","authors":"Sebastián Boy-Waxman ,&nbsp;Martin Olivier ,&nbsp;Nicolas Cermakian","doi":"10.1016/j.crpvbd.2024.100171","DOIUrl":"10.1016/j.crpvbd.2024.100171","url":null,"abstract":"<div><p>Most organisms have developed circadian clocks to adapt to 24-hour cycles in the environment. These clocks have become crucial for modulating and synchronizing complex behavioral and biological processes. A number of parasites seem to have evolved to take advantage of their hosts’ circadian rhythms to favor their own infection and survival. Some species, such as <em>Microphallus</em> sp. and <em>Trypanosoma cruzi</em>, can alter the patterns of locomotor behavior of infected intermediate hosts, which can promote transmission to a subsequent primary host. Some fungi of the genera <em>Ophiocordyceps</em> and <em>Entomophthora</em>, as well as hairworms (Nematomorpha), elicit complex behaviors that promote their host’s death at a time and place that optimizes continuation of the parasite’s life-cycle. At least in some cases, a proposed mechanism might involve a change in the expression of clock-controlled genes. Lastly, some disease-causing protozoan parasites of the genera <em>Trypanosoma</em>, <em>Plasmodium</em>, and <em>Leishmania</em> induce changes in the circadian rhythms of their primary hosts upon infection. Some of these changes may be attributed to circadian alterations resulting from the host’s inflammatory response to the infection or other unexplored responses or adaptations to the illness. Thus, a distinction must be made between manipulation of the parasite and response of the host when studying these alterations in the future. Parasitic manipulation of circadian rhythms, which vastly modulates behavior and physiology, is an essential issue that has been relatively understudied. A deeper understanding of this phenomenon could lead to the development of novel therapeutic approaches for the diseases that these parasites convey.</p></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":"5 ","pages":"Article 100171"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667114X24000025/pdfft?md5=450d349550989e85087d9517d988c999&pid=1-s2.0-S2667114X24000025-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139888302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal dynamics of the Hyalomma marginatum-borne pathogens in southern France","authors":"Charlotte Joly-Kukla ,&nbsp;Frédéric Stachurski ,&nbsp;Maxime Duhayon ,&nbsp;Clémence Galon ,&nbsp;Sara Moutailler ,&nbsp;Thomas Pollet","doi":"10.1016/j.crpvbd.2024.100213","DOIUrl":"10.1016/j.crpvbd.2024.100213","url":null,"abstract":"<div><div>Spatio-temporal scales have a clear influence on microbial community distribution and diversity and should thus be applied to study the dynamics of microorganisms. The invasive tick species <em>Hyalomma marginatum</em> has recently become established in southern France. It may carry pathogens of medical and veterinary interest including the Crimean-Congo haemorrhagic fever virus, <em>Rickettsia aeschlimannii</em>, <em>Theileria equi</em> and <em>Anaplasma phagocytophilum</em>. Pathogenic communities of <em>H. marginatum</em> have been identified and their spatial distribution characterized, but their temporal dynamics remain unknown. <em>Hyalomma marginatum</em> ticks were collected from hosts at monthly intervals from February to September 2022 in a site in southern France to study their presence and temporal dynamics. Of the 281 ticks analysed, we detected pathogens including <em>R. aeschlimannii</em>, <em>Anaplasma</em> spp. and <em>T. equi</em> with infection rates reaching 47.0%, 4.6% and 11.0%, respectively. A total of 14.6% of ticks were infected with at least <em>Theileria</em> or <em>Anaplasma</em>, with monthly fluctuations ranging from 2.9% to 28.6%. Strong temporal patterns were observed for each pathogen detected, particularly for <em>R. aeschlimannii</em>, whose infection rates increased dramatically at the beginning of summer, correlated with monthly mean temperatures at the site. Based on these results, we hypothesise that <em>R. aeschlimannii</em> may be a secondary symbiont of <em>H. marginatum</em> and could be involved in the stress response to temperature increase and mediate thermal tolerance of <em>H. marginatum</em>. Analysis of monthly and seasonal fluctuations in pathogens transmitted by <em>H. marginatum</em> led us to conclude that the risk of infection is low but persists throughout the period of <em>H. marginatum</em> activity, with a notable increase in summer.</div></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":"6 ","pages":"Article 100213"},"PeriodicalIF":1.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Helminth fauna of the black goby Gobius niger L. (Gobiiformes: Gobiidae) from the Finnish Archipelago, Baltic Sea: Molecular and morphological data","authors":"Inga Martinek ,&nbsp;Jesús S. Hernández-Orts","doi":"10.1016/j.crpvbd.2023.100169","DOIUrl":"10.1016/j.crpvbd.2023.100169","url":null,"abstract":"<div><p>Black gobies (<em>Gobius niger</em>) from the Finnish Archipelago, Baltic Sea, were screened for helminth infections in summer 2020. Helminths were identified morphologically and/or molecularly. Altogether 26 novel sequences were generated and analysed using maximum likelihood estimation. Morphological and phylogenetic analyses based on mitochondrial genes revealed the presence of 8 species belonging to the Digenea (<em>Diplostomum mergi</em> Lineage 3), Cestoda (<em>Bothriocephalus scorpii</em>), Nematoda (<em>Contracaecum rudolphii</em> A, <em>Cucullanus</em> sp. and <em>Hysterothylacium aduncum</em>), and Acanthocephala (a putative new species of <em>Corynosoma</em>, <em>Corynosoma semerme</em> and <em>Neoechinorhynchus</em> sp.). Phylogenetic and comparative sequence analyses revealed that the putative new acanthocephalan species is closely related to <em>C. neostrumosum</em> described from the Caspian seal, <em>Pusa caspica</em>, in the Caspian Sea. The black goby represents a new host record for four parasite species (<em>Diplostomum mergi</em> Lineage 3, <em>Contracaecum rudolphii</em> A, <em>Corynosoma semerme</em> and <em>Corynosoma</em> sp.). The Finnish Archipelago is a novel locality record for three species (<em>Corynosoma</em> sp., <em>Diplostomum mergi</em> Lineage 3 and <em>Bothriocephalus scorpii</em>).</p></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":"5 ","pages":"Article 100169"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667114X23000572/pdfft?md5=75f3fe78aac30287f0456f501155bd59&pid=1-s2.0-S2667114X23000572-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139187758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case of abnormal swimming patterns in juvenile Oblada melanura naturally infected with Philometra obladae (Nematoda: Philometridae) in the Tyrrhenian Sea off Sicily, Italy","authors":"Giovanni De Benedetto,&nbsp;Gabriella Gaglio","doi":"10.1016/j.crpvbd.2023.100167","DOIUrl":"10.1016/j.crpvbd.2023.100167","url":null,"abstract":"<div><p>The saddled seabream, <em>Oblada melanura</em> (L.), is a common seawater species present in the Mediterranean. Between July and August 2023, during diving activities along the Sicilian coast of Italy, we recorded with a digital camera several specimens of <em>O. melanura</em> showing an abnormal pattern of swimming in the water column. The unusual swimming behaviour was characterized by fast, uncoordinated directional changes and isolation from the remaining fishes in the shoal. Four dead fish were found and collected for necropsy and parasitological evaluation/examination. Upon gross examination, all fish showed an evident coelomic distension; the coelomic cavity of two fish was filled with nematodes that dislocated the coelomic organs, and the other two had degraded nematodes and a conspicuous quantity of fluid in the coelomic cavity. All collected parasites were identified as <em>Philometra obladae</em> (Nematoda: Philometridae) according to morphological criteria. Here, we describe the unusual swimming behaviour of <em>O. melanura</em> naturally infected with <em>Ph. obladae</em> and the results of the examination of dead fish infected with this parasite. Future studies are needed to better evaluate and describe the dynamics and the epidemiology of <em>Ph. obladae</em> infection in wild <em>O. melanura</em>.</p></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":"5 ","pages":"Article 100167"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667114X23000559/pdfft?md5=d6d5e0860b4b9bd591cca2c1e4fe0c4f&pid=1-s2.0-S2667114X23000559-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139190657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Dirofilaria immitis, Dipylidium caninum and Taenia spp. in populations of cats from shelters and research colonies, in two endemic regions of eastern Australia","authors":"Florian Roeber ,&nbsp;Riannon Apicella ,&nbsp;Michael Chambers ,&nbsp;Don Strazzeri ,&nbsp;Norbert Mencke ,&nbsp;Katrin Blazejak","doi":"10.1016/j.crpvbd.2024.100226","DOIUrl":"10.1016/j.crpvbd.2024.100226","url":null,"abstract":"<div><div>There is a paucity of available prevalence data for key parasite species and genera infecting cats in Australia. The present study aimed to determine the prevalence of key parasites infecting cats in Australia, namely the feline heartworm, <em>Dirofilaria immitis</em>, and the cestodes <em>Dipylidium caninum</em> and <em>Taenia</em> spp. The prevalence of these parasites was assessed in cat populations in five separate locations of eastern Australia and within two geographical regions: humid subtropical and oceanic. A total of 141 cats were enrolled in this study. Of these, 91 were tested for <em>D. immitis</em> by feline heartworm antigen/antibody testing, 93 samples were tested for <em>Di. caninum</em> and <em>Taenia</em> spp. by faecal floatation and a subset of 48 cat samples were tested for <em>Di. caninum</em> and <em>Taenia</em> spp. by PCR performed on peri-anal swabs. Test results were negative for the two species with a single positive result observed for <em>Taenia</em> spp. Point estimates of prevalence were therefore 0% for feline heartworm and <em>Di. caninum</em> and 1.1% for <em>Taenia</em> spp. Associated 95% confidence intervals around the point estimate were 0–4.1% for feline heartworm, 0–3.9% for <em>Di. caninum</em> and 0–5.8% for <em>Taenia</em> spp. It should be noted that estimates were calculated using nominal values for test sensitivity and specificity; however, the overall prevalence of all three parasite species in these geographical regions is likely to be minimal based on the data from this study.</div></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":"6 ","pages":"Article 100226"},"PeriodicalIF":1.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142660348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bibliometric analysis for the identification of main limitations and future directions of vaccines for the control of ticks and tick-borne pathogens in Uganda","authors":"José de la Fuente ,&nbsp;Justus Rutaisire","doi":"10.1016/j.crpvbd.2024.100175","DOIUrl":"https://doi.org/10.1016/j.crpvbd.2024.100175","url":null,"abstract":"<div><p>Ticks and tick-borne diseases (TBD) are a growing threat for human and animal health worldwide with high incidence in African countries such as Uganda where it affects cattle health and production. Considering recent advances in bibliometric analysis, in this review we used a bibliometric descriptive approach for the analysis of publications and patents in the fields of ticks, TBD, and vaccines in Uganda. The results showed that major gaps and limitations are associated with (i) low contributions from Ugandan institutions, (ii) limited international collaborations, (iii) poor impact of translational research, and (iv) little research on tick control vaccines. The results were then used to propose future directions to approach these limitations in Uganda. Although ongoing initiatives and international collaborations are contributing to address major gaps and limitations, future directions should advance in these collaborative projects together with new initiatives addressing (i) basic and translational research on TBD such as CCHF and ASF, (ii) participation of Ugandan institutions in new international consortia in this area, (iii) promoting communication of these initiatives to Ugandan cattle holders and general population to attract support from public and private sectors, (iv) stimulate and support scientific publications and patents with participation of Ugandan scientists, and (v) build and implement production capacity for vaccines in Uganda. These results contribute to guiding Ugandan scientists and national authorities to face challenges posed by ticks and TBD with implications for other African countries.</p></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":"5 ","pages":"Article 100175"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667114X24000062/pdfft?md5=5d848f01303603ba29cf78c26081e5ca&pid=1-s2.0-S2667114X24000062-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140641393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is Anopheles gambiae (sensu stricto), the principal malaria vector in Africa prone to resistance development against new insecticides? Outcomes from laboratory exposure of An. gambiae (s.s.) to sub-lethal concentrations of chlorfenapyr and clothianidin","authors":"Salum Azizi ,&nbsp;Njelembo J. Mbewe ,&nbsp;Hosiana Mo ,&nbsp;Felista Edward ,&nbsp;Godwin Sumari ,&nbsp;Silvia Mwacha ,&nbsp;Agness Msapalla ,&nbsp;Benson Mawa ,&nbsp;Franklin Mosha ,&nbsp;Johnson Matowo","doi":"10.1016/j.crpvbd.2024.100172","DOIUrl":"https://doi.org/10.1016/j.crpvbd.2024.100172","url":null,"abstract":"<div><p>Indiscriminate use of pesticides in the public health and agriculture sectors has contributed to the development of resistance in malaria vectors following exposure to sub-lethal concentrations. To preserve the efficacy of vector control tools and prevent resistance from spreading, early resistance detection is urgently needed to inform management strategies. The introduction of new insecticides for controlling malaria vectors such as clothianidin and chlorfenapyr requires research to identify early markers of resistance which could be used in routine surveillance. This study investigated phenotypic resistance of <em>Anopheles gambiae</em> (<em>sensu stricto</em>) Muleba-Kis strain using both WHO bottle and tube assays following chlorfenapyr, clothianidin, and alpha-cypermethrin selection against larvae and adults under laboratory conditions. High mortality rates were recorded for both chlorfenapyr-selected mosquitoes that were consistently maintained for 10 generations (24-h mortality of 92–100% and 72-h mortality of 98–100% for selected larvae; and 24-h mortality of 95–100% and 72-h mortality of 98–100% for selected adults). Selection with clothianidin at larval and adult stages showed a wide range of mortality (18–91%) compared to unselected progeny where mortality was approximately 99%. On the contrary, mosquitoes selected with alpha-cypermethrin from the adult selection maintained low mortality (28% at Generation 2 and 23% at Generation 4) against discrimination concentration compared to unselected progeny where average mortality was 51%. The observed resistance in the clothianidin-selected mosquitoes needs further investigation to determine the underlying resistance mechanism against this insecticide class. Additionally, further investigation is recommended to develop molecular markers for observed clothianidin phenotypic resistance.</p></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":"5 ","pages":"Article 100172"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667114X24000037/pdfft?md5=f2145e1449b397b1c7a1882c512a67af&pid=1-s2.0-S2667114X24000037-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139999307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}