Biochimica et biophysica acta最新文献

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Die abhängigkeit der jodakkumulation in schilddrüsenschnitten von der ionalen zusammensetzung des inkubationsmediums und ihre beeinflussung durch herzglykoside 碘片效果由病毒母体的原液组成,再由心甘甘油的作用作用
Biochimica et biophysica acta Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91037-0
H.W. Iff, W. Wilbrandt
{"title":"Die abhängigkeit der jodakkumulation in schilddrüsenschnitten von der ionalen zusammensetzung des inkubationsmediums und ihre beeinflussung durch herzglykoside","authors":"H.W. Iff,&nbsp;W. Wilbrandt","doi":"10.1016/0006-3002(63)91037-0","DOIUrl":"10.1016/0006-3002(63)91037-0","url":null,"abstract":"<div><p>The influence of the external Na<sup>+</sup> and K<sup>+</sup> concentration and of ouabain on I<sup>−</sup> uptake and release was studied in cow-thyroid slices blocked with propylthiouracil. It could be shown that the I<sup>−</sup> transport is dependent on the presence of both Na<sup>+</sup> and K<sup>+</sup> ions. However, the dependence on Na<sup>+</sup> seems to be more direct: the effect of removal of K<sup>+</sup> and of the application of ouabain set in only after a latency period while the effect of Na<sup>+</sup> removal was immediate. The half-time of I<sup>−</sup> release due to removal of Na<sup>+</sup> from the medium was markedly shorter than that due to K<sup>+</sup> removal or that of the release caused by ouabain. However, the half-times in the latter two cases were similar. A thyroid slice kept for a long time under ouabain poisoning in a solution wherein 90% of the Na<sup>+</sup> was replaced by Li<sup>+</sup> was still able to accumulate I<sup>−</sup> for a short time when brought back into a medium of normal Na<sup>+</sup> content in spite of the uninterrupted presence of ouabain. The possible connections between the K<sup>+</sup>-Na<sup>+</sup> transport mechanism and the I<sup>−</sup> transport are discussed.</p><p>In principle two basically different relations can be thought of. Either the I<sup>−</sup> transport is directly coupled with the Na<sup>+</sup>K<sup>+</sup> exchange mechanism or the cation-concentration differences created by the Na<sup>+</sup>K<sup>+</sup> transport system are a prerequisite for the I<sup>−</sup> transport. The present results are taken to favour the latter assumption.</p></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Pages 711-725"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91037-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23673344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
Changes in D-glucaric acid excretion induced by stimulators of ascorbic acid biosynthesis 抗坏血酸生物合成刺激剂诱导d -葡萄糖酸排泄的变化
Biochimica et biophysica acta Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91038-2
C.A. Marsh, Lorna M. Reid
{"title":"Changes in D-glucaric acid excretion induced by stimulators of ascorbic acid biosynthesis","authors":"C.A. Marsh,&nbsp;Lorna M. Reid","doi":"10.1016/0006-3002(63)91038-2","DOIUrl":"10.1016/0006-3002(63)91038-2","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Pages 726-728"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91038-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23673345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 36
Vorkommen von Protocatechualdehyd bei einem fall von bösartigem phäochromocytom 有恶性p2x次品
Biochimica et biophysica acta Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91047-3
P. Herrlich, C.E. Sekeris
{"title":"Vorkommen von Protocatechualdehyd bei einem fall von bösartigem phäochromocytom","authors":"P. Herrlich,&nbsp;C.E. Sekeris","doi":"10.1016/0006-3002(63)91047-3","DOIUrl":"10.1016/0006-3002(63)91047-3","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Page 750"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91047-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23673352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
The reaction of l-cysteine with all-trans-retinene l-半胱氨酸与全反式维甲酸的反应
Biochimica et biophysica acta Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91048-5
James C. Peskin, Bruce B. Love
{"title":"The reaction of l-cysteine with all-trans-retinene","authors":"James C. Peskin,&nbsp;Bruce B. Love","doi":"10.1016/0006-3002(63)91048-5","DOIUrl":"10.1016/0006-3002(63)91048-5","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Pages 751-753"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91048-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23673145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
Comparison of the incorporation of tyrosine and its iodinated analogs into the proteins of Ehrlich ascites tumor cells and rat-liver slices 酪氨酸及其碘化类似物掺入埃利希腹水肿瘤细胞和大鼠肝片蛋白质的比较
Biochimica et biophysica acta Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91051-5
Hugues J.-P. Ryser
{"title":"Comparison of the incorporation of tyrosine and its iodinated analogs into the proteins of Ehrlich ascites tumor cells and rat-liver slices","authors":"Hugues J.-P. Ryser","doi":"10.1016/0006-3002(63)91051-5","DOIUrl":"10.1016/0006-3002(63)91051-5","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Pages 759-762"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91051-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23673148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
Phosphopyruvate carboxylase in liver tumors of different growth rates 不同生长速度肝脏肿瘤的磷酸丙酮酸羧化酶
Biochimica et biophysica acta Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91061-8
S.R. Wagle , H.P. Morris , George Weber
{"title":"Phosphopyruvate carboxylase in liver tumors of different growth rates","authors":"S.R. Wagle ,&nbsp;H.P. Morris ,&nbsp;George Weber","doi":"10.1016/0006-3002(63)91061-8","DOIUrl":"10.1016/0006-3002(63)91061-8","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Pages 783-785"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91061-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23673158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
The metabolism of γ-hydroxyglutamate in rat liver II. A transaminase concerned in γ-hydroxyglutamate metabolism γ-羟谷氨酸在大鼠肝脏中的代谢ⅱ。一种与γ-羟谷氨酸代谢有关的转氨酶
Biochimica et biophysica acta Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91028-X
Kazuoki Kuratomi, Keiko Fukunaga, Yasuko Kobayashi
{"title":"The metabolism of γ-hydroxyglutamate in rat liver II. A transaminase concerned in γ-hydroxyglutamate metabolism","authors":"Kazuoki Kuratomi,&nbsp;Keiko Fukunaga,&nbsp;Yasuko Kobayashi","doi":"10.1016/0006-3002(63)91028-X","DOIUrl":"10.1016/0006-3002(63)91028-X","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. An enzyme which catalyses the transamination between γ-hydroxyglutamate and α-ketoglutarate was extracted from rat-liver acetone powder and purified in excess of 15-fold. Fractionation of the enzyme from a separate aspartate transaminase (<span>L</span>-aspartate: 2-oxoglutarate aminotransferase, EC 2.6.1.1) remained incomplete.</p></span></li><li><span>2.</span><span><p>2. [2-<sup>14</sup>C]Pyruvate was incorporated into γ-hydroxy-α-ketoglutarate and γ-hydroxyglutamate in the presence of glyoxylate in rat-liver homogenate. Specific activity values of these three compounds indicated the occurence of the following pathway: Pyruvate + glyoxylate ⇌ γ-hydroxy-α-ketoglutarate ⇌ γ-hydroxyglutamate.</p></span></li><li><span>3.</span><span><p>3. A further possible pathway of γ-hydroxy-α-ketoglutarate metabolism leading to malate and of γ-hydroxyglutamate to its decarboxylation product or to <span>L</span>-hydroxyproline were inferred and discussed.</p></span></li></ul></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Pages 629-636"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91028-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23673336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Biosynthesis of ubiquinone and ubichromenol 泛醌和异丙酚的生物合成
Biochimica et biophysica acta Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91043-6
J. Green, A.T. Diplock, J. Bunyan, D. McHale
{"title":"Biosynthesis of ubiquinone and ubichromenol","authors":"J. Green,&nbsp;A.T. Diplock,&nbsp;J. Bunyan,&nbsp;D. McHale","doi":"10.1016/0006-3002(63)91043-6","DOIUrl":"10.1016/0006-3002(63)91043-6","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Pages 739-741"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91043-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23673349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Enzyme reactions with phenolic compounds: a meta-O-methyltransferase in plants 与酚类化合物的酶反应:植物中的一种甲基转移酶
Biochimica et biophysica acta Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91046-1
Bernard J. Finkle, Richard F. Nelson
{"title":"Enzyme reactions with phenolic compounds: a meta-O-methyltransferase in plants","authors":"Bernard J. Finkle,&nbsp;Richard F. Nelson","doi":"10.1016/0006-3002(63)91046-1","DOIUrl":"10.1016/0006-3002(63)91046-1","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Pages 747-749"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91046-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74551021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 64
Non-phosphorylated intermediates in oxidative phosphorylation 氧化磷酸化中的非磷酸化中间体
Biochimica et biophysica acta Pub Date : 1963-11-15 DOI: 10.1016/0006-3002(63)90902-8
Pierre V. Vignais
{"title":"Non-phosphorylated intermediates in oxidative phosphorylation","authors":"Pierre V. Vignais","doi":"10.1016/0006-3002(63)90902-8","DOIUrl":"10.1016/0006-3002(63)90902-8","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. This paper is concerned with the study of the formation of non-phosphorylated intermediates in digitonin particles prepared from rat-liver mitochondria.</p></span></li><li><span>2.</span><span><p>2. Digitonin particles were preincubated with either β-hydroxybutyrate, sucinate or ferrocytochrome <em>c</em> for a short time at 20°. After cooling the preincubated mixture was incubated at 1° with <sup>32</sup>P<sub>i</sub> and ADP. Under these conditions, the time course of [<sup>32</sup>P]ATP synthesis is represented by a biphasic curve. The initial portion corresponds to a high rate of [<sup>32</sup>P]ATP synthesis whereas the second portion corresponds to a slower rate.</p></span></li><li><span>3.</span><span><p>3. The rapid initial [<sup>32</sup>P]ATP synthesis is maximal for pH values ranging from 6.5 to 7.3. It is increased by compounds which increase the efficiency of oxidative phosphorylation in digitonin particles such as EDTA and serum albumin and is inhibited by inhibitors of oxidative phosphorylation such as 2,4-dinitrophenol or azide. It is delayed or considerably depressed when unlabeled P<sub>i</sub> is added during the preincubation time prior to the addition of <sup>32</sup>P<sub>i</sub> and ADP but is not affected by atractylate, a compound which is capable of specifically inhibiting the ATP-P<sub>i</sub> exchange in digitonin particles without interfering with the ATP synthesis linked to respiration. Finally it is enhanced by low concentrations of oligomycin which at 1° have little effect on the rate and the yield of oxidative phosphorylation.</p></span></li><li><span>4.</span><span><p>4. The effect of <sup>32</sup>P<sub>i</sub> concentration on the initial rate of [<sup>32</sup>P]ATP synthesis was determined: <em>K</em><sub>m</sub> = 1.7·10<sup>−3</sup> M.</p></span></li><li><span>5.</span><span><p>5. The experimental findings have been interpreted as follows: non-phosphorylated intermediates accumulate in digitonin particles during the oxidation of β-hydroxybutyrate, succinate or ferrocytochrome <em>c</em>. These intermediates are discharged upon addition of P<sub>i</sub> and ADP, the net result of the reaction being the synthesis of ATP.</p></span></li></ul></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 3","pages":"Pages 404-419"},"PeriodicalIF":0.0,"publicationDate":"1963-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)90902-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23670740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
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