{"title":"Ascorbic acid-induced fluorescence of a noradrenaline oxidation product","authors":"W.H. Harrison","doi":"10.1016/0006-3002(63)91036-9","DOIUrl":"10.1016/0006-3002(63)91036-9","url":null,"abstract":"<div><p>Ascorbic acid has been found to induce the fluorescence of an intermediate oxidation product of noradrenaline which is formed in a ferricyanide oxidation. The fluorescence of the product is a function of the ascorbic acid and noradrenaline concentration and at conditions at which the fluorescence of the noradrenaline product is optimal the fluorescence of adrenaline, tested similarly, in negligible. The reaction has a definite pH optimum (pH 6) and the fluorescence of the final product depends on the time of the oxidation. The stability and rate of formation of the noradrenaline product is dependent upon the concentration level of the ascorbic acid and ferricyanide. The reaction shows promise as the basis for a new noradrenaline assay.</p></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Pages 705-710"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91036-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23673343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Die abhängigkeit der jodakkumulation in schilddrüsenschnitten von der ionalen zusammensetzung des inkubationsmediums und ihre beeinflussung durch herzglykoside","authors":"H.W. Iff, W. Wilbrandt","doi":"10.1016/0006-3002(63)91037-0","DOIUrl":"10.1016/0006-3002(63)91037-0","url":null,"abstract":"<div><p>The influence of the external Na<sup>+</sup> and K<sup>+</sup> concentration and of ouabain on I<sup>−</sup> uptake and release was studied in cow-thyroid slices blocked with propylthiouracil. It could be shown that the I<sup>−</sup> transport is dependent on the presence of both Na<sup>+</sup> and K<sup>+</sup> ions. However, the dependence on Na<sup>+</sup> seems to be more direct: the effect of removal of K<sup>+</sup> and of the application of ouabain set in only after a latency period while the effect of Na<sup>+</sup> removal was immediate. The half-time of I<sup>−</sup> release due to removal of Na<sup>+</sup> from the medium was markedly shorter than that due to K<sup>+</sup> removal or that of the release caused by ouabain. However, the half-times in the latter two cases were similar. A thyroid slice kept for a long time under ouabain poisoning in a solution wherein 90% of the Na<sup>+</sup> was replaced by Li<sup>+</sup> was still able to accumulate I<sup>−</sup> for a short time when brought back into a medium of normal Na<sup>+</sup> content in spite of the uninterrupted presence of ouabain. The possible connections between the K<sup>+</sup>-Na<sup>+</sup> transport mechanism and the I<sup>−</sup> transport are discussed.</p><p>In principle two basically different relations can be thought of. Either the I<sup>−</sup> transport is directly coupled with the Na<sup>+</sup>K<sup>+</sup> exchange mechanism or the cation-concentration differences created by the Na<sup>+</sup>K<sup>+</sup> transport system are a prerequisite for the I<sup>−</sup> transport. The present results are taken to favour the latter assumption.</p></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Pages 711-725"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91037-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23673344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Changes in D-glucaric acid excretion induced by stimulators of ascorbic acid biosynthesis","authors":"C.A. Marsh, Lorna M. Reid","doi":"10.1016/0006-3002(63)91038-2","DOIUrl":"10.1016/0006-3002(63)91038-2","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Pages 726-728"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91038-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23673345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Vorkommen von Protocatechualdehyd bei einem fall von bösartigem phäochromocytom","authors":"P. Herrlich, C.E. Sekeris","doi":"10.1016/0006-3002(63)91047-3","DOIUrl":"10.1016/0006-3002(63)91047-3","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Page 750"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91047-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23673352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The reaction of l-cysteine with all-trans-retinene","authors":"James C. Peskin, Bruce B. Love","doi":"10.1016/0006-3002(63)91048-5","DOIUrl":"10.1016/0006-3002(63)91048-5","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Pages 751-753"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91048-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23673145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of the incorporation of tyrosine and its iodinated analogs into the proteins of Ehrlich ascites tumor cells and rat-liver slices","authors":"Hugues J.-P. Ryser","doi":"10.1016/0006-3002(63)91051-5","DOIUrl":"10.1016/0006-3002(63)91051-5","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Pages 759-762"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91051-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23673148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phosphopyruvate carboxylase in liver tumors of different growth rates","authors":"S.R. Wagle , H.P. Morris , George Weber","doi":"10.1016/0006-3002(63)91061-8","DOIUrl":"10.1016/0006-3002(63)91061-8","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Pages 783-785"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91061-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23673158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The metabolism of γ-hydroxyglutamate in rat liver II. A transaminase concerned in γ-hydroxyglutamate metabolism","authors":"Kazuoki Kuratomi, Keiko Fukunaga, Yasuko Kobayashi","doi":"10.1016/0006-3002(63)91028-X","DOIUrl":"10.1016/0006-3002(63)91028-X","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. An enzyme which catalyses the transamination between γ-hydroxyglutamate and α-ketoglutarate was extracted from rat-liver acetone powder and purified in excess of 15-fold. Fractionation of the enzyme from a separate aspartate transaminase (<span>L</span>-aspartate: 2-oxoglutarate aminotransferase, EC 2.6.1.1) remained incomplete.</p></span></li><li><span>2.</span><span><p>2. [2-<sup>14</sup>C]Pyruvate was incorporated into γ-hydroxy-α-ketoglutarate and γ-hydroxyglutamate in the presence of glyoxylate in rat-liver homogenate. Specific activity values of these three compounds indicated the occurence of the following pathway: Pyruvate + glyoxylate ⇌ γ-hydroxy-α-ketoglutarate ⇌ γ-hydroxyglutamate.</p></span></li><li><span>3.</span><span><p>3. A further possible pathway of γ-hydroxy-α-ketoglutarate metabolism leading to malate and of γ-hydroxyglutamate to its decarboxylation product or to <span>L</span>-hydroxyproline were inferred and discussed.</p></span></li></ul></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Pages 629-636"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91028-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23673336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biosynthesis of ubiquinone and ubichromenol","authors":"J. Green, A.T. Diplock, J. Bunyan, D. McHale","doi":"10.1016/0006-3002(63)91043-6","DOIUrl":"10.1016/0006-3002(63)91043-6","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Pages 739-741"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91043-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23673349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Enzyme reactions with phenolic compounds: a meta-O-methyltransferase in plants","authors":"Bernard J. Finkle, Richard F. Nelson","doi":"10.1016/0006-3002(63)91046-1","DOIUrl":"10.1016/0006-3002(63)91046-1","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Pages 747-749"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91046-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74551021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}