Biochimica et biophysica acta最新文献_第4页

The optical rotatory dispersion of myosin a IV. Conformational changes in meromyosins 肌凝蛋白的旋光色散。肌凝蛋白的构象变化

Biochimica et biophysica acta Pub Date : 1963-12-13 Epub Date: 2003-02-09 DOI: 10.1016/0006-3002(63)91034-5

Yuji Tonomura , Kazuko Sekiya , Kiichi Imamura

{"title":"The optical rotatory dispersion of myosin a IV. Conformational changes in meromyosins","authors":"Yuji Tonomura , Kazuko Sekiya , Kiichi Imamura","doi":"10.1016/0006-3002(63)91034-5","DOIUrl":"10.1016/0006-3002(63)91034-5","url":null,"abstract":"<div>The effect of 8% dioxane on the optical rotatory dispersion of myosin A purified by treatment with DEAE-cellulose was measured in 0.6 M KCl and at pH 7.0 and 20°. The increase in the —b0 term by 5% was followed by a gradual decrease to 0.88 of the original. The —b0 term of L1-meromyosin in 0.6 M KCl and at pH 7.0 and 20% was 575 and was unchanged by the addition of dioxane, ATP of PP1. On the other hand, the —b0 term of H2-meromyosin in 0.3 M KCl and at pH 7.0 and 20% was 263. It increased by several percent immediately after the addition of 8% dioxane and decreased gradually with time. It increased by several percent on the addition of 4 moles p-chloromercuribenzoate, but decreased by several percent on the addition of 8 moles p-chloromercuribenzoate per 105 g protein. It was also decreased by the binding of H2-meromyosin with F-actin. ATP increased the —b0 term of H2-meromyosin by several percent, though PP1 decreased the term by several percent. Thus these reagents change the helical content of H2-meromyosin in ways similar to the case of myosin A. The extents of the changes in the helical content of H2-meromyosin by F-actin and ATP were, however, higher than those observed in myosin A.</div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Pages 690-697"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91034-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23673341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Study of [1-14C]glycine incorporation into extractable and residual glycogen of guinea-pig liver in vivo [1-14C]甘氨酸在豚鼠肝脏可提取和残留糖原中的掺入研究

Biochimica et biophysica acta Pub Date : 1963-12-13 Epub Date: 2003-02-09 DOI: 10.1016/0006-3002(63)91045-X

Vishwa Nath Singh , T.A. Venkitasubramanian

引用次数: 0

Action de différents agents réducteurs sur le spectre, à basse température, de fractions subcellulaires de levure 不同还原剂对酵母亚细胞组分低温谱的影响

Biochimica et biophysica acta Pub Date : 1963-12-13 Epub Date: 2003-02-09 DOI: 10.1016/0006-3002(63)91052-7

Thérèse Heyman-Blanchet

引用次数: 4

On the internal pH value of bacteria 细菌内部的pH值

Biochimica et biophysica acta Pub Date : 1963-12-13 Epub Date: 2003-02-09 DOI: 10.1016/0006-3002(63)91053-9

Lawrence J. Kadish, Arthur B. Pardee

引用次数: 8

The effect of oxygen on the composition and organisation of the electron transport system of yeast 氧对酵母电子传递系统组成和组织的影响

Biochimica et biophysica acta Pub Date : 1963-12-13 Epub Date: 2003-02-09 DOI: 10.1016/0006-3002(63)91062-X

D.R. Biggs, Anthony W. Linnane

引用次数: 33

Changes in free and acetylated amino acids during tobacco mosaic virus multiplication 烟草花叶病毒增殖过程中游离氨基酸和乙酰化氨基酸的变化

Biochimica et biophysica acta Pub Date : 1963-12-13 Epub Date: 2003-02-09 DOI: 10.1016/0006-3002(63)91030-8

Marvin Karasek

引用次数: 6

Ascorbic acid-induced fluorescence of a noradrenaline oxidation product 抗坏血酸诱导的去甲肾上腺素氧化产物的荧光

Biochimica et biophysica acta Pub Date : 1963-12-13 Epub Date: 2003-02-09 DOI: 10.1016/0006-3002(63)91036-9

W.H. Harrison

引用次数: 4

Die abhängigkeit der jodakkumulation in schilddrüsenschnitten von der ionalen zusammensetzung des inkubationsmediums und ihre beeinflussung durch herzglykoside 碘片效果由病毒母体的原液组成，再由心甘甘油的作用作用

Biochimica et biophysica acta Pub Date : 1963-12-13 Epub Date: 2003-02-09 DOI: 10.1016/0006-3002(63)91037-0

H.W. Iff, W. Wilbrandt

{"title":"Die abhängigkeit der jodakkumulation in schilddrüsenschnitten von der ionalen zusammensetzung des inkubationsmediums und ihre beeinflussung durch herzglykoside","authors":"H.W. Iff, W. Wilbrandt","doi":"10.1016/0006-3002(63)91037-0","DOIUrl":"10.1016/0006-3002(63)91037-0","url":null,"abstract":"<div>The influence of the external Na+ and K+ concentration and of ouabain on I− uptake and release was studied in cow-thyroid slices blocked with propylthiouracil. It could be shown that the I− transport is dependent on the presence of both Na+ and K+ ions. However, the dependence on Na+ seems to be more direct: the effect of removal of K+ and of the application of ouabain set in only after a latency period while the effect of Na+ removal was immediate. The half-time of I− release due to removal of Na+ from the medium was markedly shorter than that due to K+ removal or that of the release caused by ouabain. However, the half-times in the latter two cases were similar. A thyroid slice kept for a long time under ouabain poisoning in a solution wherein 90% of the Na+ was replaced by Li+ was still able to accumulate I− for a short time when brought back into a medium of normal Na+ content in spite of the uninterrupted presence of ouabain. The possible connections between the K+-Na+ transport mechanism and the I− transport are discussed.In principle two basically different relations can be thought of. Either the I− transport is directly coupled with the Na+K+ exchange mechanism or the cation-concentration differences created by the Na+K+ transport system are a prerequisite for the I− transport. The present results are taken to favour the latter assumption.</div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 4","pages":"Pages 711-725"},"PeriodicalIF":0.0,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91037-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23673344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Changes in D-glucaric acid excretion induced by stimulators of ascorbic acid biosynthesis 抗坏血酸生物合成刺激剂诱导d -葡萄糖酸排泄的变化

Biochimica et biophysica acta Pub Date : 1963-12-13 Epub Date: 2003-02-09 DOI: 10.1016/0006-3002(63)91038-2

C.A. Marsh, Lorna M. Reid

引用次数: 36

Metabolites of p-aminobenzoic acid III. A metabolic pathway of p-aminobenzoic acid resulting in the sequential formation of p-aminobenzyl alcohol and p-hydroxyaniline 对氨基苯甲酸代谢物III。对氨基苯甲酸的代谢途径，导致对氨基苯甲酸醇和对羟基苯胺的顺序形成

Biochimica et biophysica acta Pub Date : 1963-12-13 Epub Date: 2003-02-09 DOI: 10.1016/0006-3002(63)91024-2

N.H. Sloane, K.G. Untch, A.W. Johnson

引用次数: 9