Ascorbic acid-induced fluorescence of a noradrenaline oxidation product

W.H. Harrison
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引用次数: 4

Abstract

Ascorbic acid has been found to induce the fluorescence of an intermediate oxidation product of noradrenaline which is formed in a ferricyanide oxidation. The fluorescence of the product is a function of the ascorbic acid and noradrenaline concentration and at conditions at which the fluorescence of the noradrenaline product is optimal the fluorescence of adrenaline, tested similarly, in negligible. The reaction has a definite pH optimum (pH 6) and the fluorescence of the final product depends on the time of the oxidation. The stability and rate of formation of the noradrenaline product is dependent upon the concentration level of the ascorbic acid and ferricyanide. The reaction shows promise as the basis for a new noradrenaline assay.

抗坏血酸诱导的去甲肾上腺素氧化产物的荧光
抗坏血酸已被发现能诱导在铁氰化物氧化中形成的去甲肾上腺素的中间氧化产物的荧光。产品的荧光是抗坏血酸和去甲肾上腺素浓度的函数,在去甲肾上腺素产品荧光最佳的条件下,同样测试,肾上腺素的荧光可以忽略不计。该反应有一个确定的最佳pH值(pH 6),最终产物的荧光取决于氧化的时间。去甲肾上腺素产物的稳定性和形成速率取决于抗坏血酸和铁氰化物的浓度水平。该反应有望作为一种新的去甲肾上腺素测定法的基础。
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