Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy最新文献

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Designing fluorescent covalent organic frameworks through regulation of link bond for selective detection of Al3+ and Ce3. 通过调节连接键设计用于选择性检测Al3+和Ce3的荧光共价有机框架。
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Pub Date : 2025-03-15 Epub Date: 2024-12-19 DOI: 10.1016/j.saa.2024.125620
Yingwei Gao, Shuo Zhang, Bo Ge, Hui Zhao, Chuanyu Jin, Hui Yan, Limin Zhao
{"title":"Designing fluorescent covalent organic frameworks through regulation of link bond for selective detection of Al<sup>3+</sup> and Ce<sup>3</sup>.","authors":"Yingwei Gao, Shuo Zhang, Bo Ge, Hui Zhao, Chuanyu Jin, Hui Yan, Limin Zhao","doi":"10.1016/j.saa.2024.125620","DOIUrl":"10.1016/j.saa.2024.125620","url":null,"abstract":"<p><p>The high thermal stability and chemical durability of amide-linked covalent organic frameworks (amide COFs) make them a promising material for a range of new applications. Nevertheless, the low reversibility of the amide bond presents a significant challenge to the direct synthesis of amide-bonded COFs. In this paper, we present a simple method for synthesizing amide COFs. The synthesis of imine-linked COFs was initially achieved through the reaction of 2,4,6-tris(4-aminophenyl)-1,3,5-triazine and 2,5-dimethoxybenzene-1,4-dicarboxaldehyde. Subsequently, amide COFs were synthesized via the oxidation of the imine bond into an amide bond, utilizing ammonium persulfate as the oxidizing agent. Due to the difference of link bond, two COFs separately displayed distinct and significant fluorescence enhancement for Al<sup>3+</sup> and Ce<sup>3+</sup>, which was highly sensitive and less affected by environmental factors. The strategy offers a novel approach to the convenient and environmentally benign synthesis of amide COFs, which may facilitate their wider applications.</p>","PeriodicalId":94213,"journal":{"name":"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy","volume":"329 ","pages":"125620"},"PeriodicalIF":0.0,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142911174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ultrasensitive analysis of the commonly abused CNS antitussive, dextromethorphan in biological fluids and dosage forms using a novel micellar-sensitized spectrofluorimetric approach: Compliance with greenness and blueness metrics. 利用新型胶束敏化光谱荧光法对生物液体和剂型中常被滥用的中枢神经系统止咳药右美沙芬进行超灵敏分析:符合绿色和蓝色度量标准。
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Pub Date : 2025-03-15 Epub Date: 2024-12-19 DOI: 10.1016/j.saa.2024.125644
Aya Saad Radwan, Mohamed A El Hamd, Mahmoud El-Maghrabey, Lateefa A Al-Khateeb, Wael A Mahdi, Sultan Alshehri, Wejdan T Alsaggaf, Safaa F Saleh, Angum M M Ibrahim, Bandar R Alsehli, Galal Magdy
{"title":"Ultrasensitive analysis of the commonly abused CNS antitussive, dextromethorphan in biological fluids and dosage forms using a novel micellar-sensitized spectrofluorimetric approach: Compliance with greenness and blueness metrics.","authors":"Aya Saad Radwan, Mohamed A El Hamd, Mahmoud El-Maghrabey, Lateefa A Al-Khateeb, Wael A Mahdi, Sultan Alshehri, Wejdan T Alsaggaf, Safaa F Saleh, Angum M M Ibrahim, Bandar R Alsehli, Galal Magdy","doi":"10.1016/j.saa.2024.125644","DOIUrl":"10.1016/j.saa.2024.125644","url":null,"abstract":"<p><p>The current study introduces the first micellar-enhanced spectrofluorimetric approach for the estimation of the commonly abused CNS antitussive, dextromethorphan (DXM) in its syrup and biological fluids. A micellar solution of sodium dodecyl sulfate (SDS) containing DXM showed high native fluorescence emission at 305 nm following excitation at 224 nm. Using SDS as a micellar system resulted in about a 2.5-fold increase in the drug's fluorescence intensity and quantum yield as well as the sensitivity of the approach. A thorough investigation was conducted into the experimental factors affecting the studied drug's spectrofluorimetric behavior. Additionally, the quantum yield of DXM was calculated, and it was found to reach up to 22 %. A calibration plot with a straight line was produced across the concentration range of 10.0-200.0 ng/mL. The suggested approach demonstrated excellent sensitivity down to the nanogram level, with 1.80 ng/mL for the detection limit and 5.47 ng/mL for the quantification limit. The drug under study was successfully analyzed in syrup using the designed approach, which yielded low %RSD values (≤0.882) and high %recoveries (99.20-101.00). The efficacy of the suggested fluorimetric technique in detecting DXM in human plasma and urine samples has been demonstrated with excellent recovery (98.12-101.35) and %RSD (≤1.39) values owing to its high sensitivity and selectivity. As DXM is one of the most commonly abused CNS antitussives, the capacity of the proposed method for its analysis in biological fluids can provide further insights for monitoring its potential abuse. The excellent greenness and eco-friendliness of the method were confirmed using GAPI and AGREE metrics, while the BAGI tool assessed its economy, practicality, and applicability. The method was fully validated according to ICH Q2 (R2) guidelines.</p>","PeriodicalId":94213,"journal":{"name":"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy","volume":"329 ","pages":"125644"},"PeriodicalIF":0.0,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unravelling hidden text and figures in paper-based archival documents with micro-spatially offset Raman spectroscopy imaging. 利用微空间偏移拉曼光谱成像揭开纸质档案文件中隐藏的文本和数字。
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Pub Date : 2025-03-15 Epub Date: 2024-12-11 DOI: 10.1016/j.saa.2024.125591
Marc Vermeulen, Claudia Conti, Pavel Matousek, Lora V Angelova, Alessandra Botteon
{"title":"Unravelling hidden text and figures in paper-based archival documents with micro-spatially offset Raman spectroscopy imaging.","authors":"Marc Vermeulen, Claudia Conti, Pavel Matousek, Lora V Angelova, Alessandra Botteon","doi":"10.1016/j.saa.2024.125591","DOIUrl":"10.1016/j.saa.2024.125591","url":null,"abstract":"<p><p>The preservation of paper-based archival documents is crucial for safeguarding historical and cultural heritage. Some records possess visually inaccessible text or images because of previous conservation measures, their method of construction, or historic damage. Micro-spatially Offset Raman Spectroscopy (micro-SORS) has emerged as a promising method for probing below or through opaque material substrates non-invasively. This study explores the potential of micro-SORS to image hidden text and figures in paper-based archival documents, utilizing Raman signals, fluorescence emissions, and overall spectral intensity reflecting also sample absorption. We present case studies involving sealed letters and playing cards from historical collections, demonstrating the efficacy of micro-SORS in identifying pigments and deciphering hidden ink writings. Results show the successful mapping of vermilion pigment in playing cards and reconstruction of hidden iron gall ink text in sealed letters. Chemometric analysis further enhances the visualization of hidden text. Despite challenges such as the absence of Raman signal of the target materials, micro-SORS proves to be a valuable tool for accessing hidden information in paper-based artifacts, aiding in preservation efforts and historical research.</p>","PeriodicalId":94213,"journal":{"name":"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy","volume":"329 ","pages":"125591"},"PeriodicalIF":0.0,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fluorescent biosensor for ultra-stability detection of Pax-5a based on a double cascade amplification strategy. 基于双级联扩增策略的Pax-5a超稳定检测荧光生物传感器
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Pub Date : 2025-03-15 Epub Date: 2024-12-18 DOI: 10.1016/j.saa.2024.125632
Chang Liu, Xinyi Zhang, Jun Xu, Min Gao, Suqin Wang, Hongbo Li
{"title":"Fluorescent biosensor for ultra-stability detection of Pax-5a based on a double cascade amplification strategy.","authors":"Chang Liu, Xinyi Zhang, Jun Xu, Min Gao, Suqin Wang, Hongbo Li","doi":"10.1016/j.saa.2024.125632","DOIUrl":"10.1016/j.saa.2024.125632","url":null,"abstract":"<p><p>The development of B-lymphoblastic leukemia is tightly associated with aberrant expression of Pax-5a. This work presented a novel dual signal amplification strategy-based Pax-5a detection method by combining the rolling circle amplification reaction (RCA) and the Entropy-driven toehold-mediated strand displacement (ETSD). Particularly noteworthy is the employed ETSD, which effectively improves the rate and stability of the reaction due to its unique entropy-driven principle. The uniqueness of this method is the combination of two amplification techniques, each utilizing its own strengths to achieve our intended purpose. This sensing method has been effectively used to determine the Pax-5a gene which with a reliable linear correlation for detection within a range and achieving a detection limit of 3.34 pM, calculated using the formula (3σ/S). Furthermore, even in 1 % of human serum samples, the biosensor can identify the target gene with exceptional sensitivity. The recovery rates fall within the range of 96.68-101.76 %, with a relative standard deviation (RSD) of 5.47 %. The method has a strong specificity based on sequence-specific hybridization of nucleic acids, thereby effectively preventing potential false-positive results. This fluorescent biosensor has a high detection capability for Pax-5a, and offers stable results. It provides a new way for early clinical diagnosis of acute lymphoblastic leukemia.</p>","PeriodicalId":94213,"journal":{"name":"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy","volume":"329 ","pages":"125632"},"PeriodicalIF":0.0,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142879215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sunlight-induced degradation of squarazine-based cyanide complex through imine CN free rotation: Sensing, binding and degradation studies of anionic complex. 日光通过亚胺 CN 自由旋转诱导方嗪基氰化物络合物降解:阴离子络合物的传感、结合和降解研究。
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Pub Date : 2025-02-15 Epub Date: 2024-11-12 DOI: 10.1016/j.saa.2024.125404
Poomanirajeshwari Kathirvelu, Murugan Gowripriya, Ariputhiran Muthulakshmi, Vanthana Jeyasingh, Kumaresan Murugesan, Sudha Lakshminarayanan, Selvapalam Narayanan, Lakshminarayanan Piramuthu
{"title":"Sunlight-induced degradation of squarazine-based cyanide complex through imine CN free rotation: Sensing, binding and degradation studies of anionic complex.","authors":"Poomanirajeshwari Kathirvelu, Murugan Gowripriya, Ariputhiran Muthulakshmi, Vanthana Jeyasingh, Kumaresan Murugesan, Sudha Lakshminarayanan, Selvapalam Narayanan, Lakshminarayanan Piramuthu","doi":"10.1016/j.saa.2024.125404","DOIUrl":"10.1016/j.saa.2024.125404","url":null,"abstract":"<p><p>Sunlight-induced degradable squarazine based electron deficient receptor 3,4-bis((E)-2-((perfluorophenyl)methylene)hydrazinyl)cyclobut-3-ene-1,2-dione, L has been reported here. Naked-eye colorimetric analysis, UV-Vis, IR and <sup>1</sup>H, 19F, <sup>31</sup>P-NMR spectrometric results show that this receptor L high affinity with cyanide anion. The strength of the receptor L towards colorimetrically responded anions are calculated by UV-Vis spectrometric titrations and it is found to be 9.9597 × 10<sup>3</sup> for cyanide. Interestingly, upon exposure of those anionic complexes under sunlight, the colors of those respective anionic complexes are disappeared. From this result, it is clear that these anionic complexes are capable to discharge the bound anion via CN free rotation. As evidenced from spectroscopic and colorimetric results, it is also clear that this anionic complex is not only release the bound anion, but also undergone self-degradation upon sunlight exposure. To the best of our knowledge, this is the first example for cyanide sensing of anion accomplished with self-degradation of anion complex upon exposure on sunlight.</p>","PeriodicalId":94213,"journal":{"name":"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy","volume":"327 ","pages":"125404"},"PeriodicalIF":0.0,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142645312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Novel amino-functionalized MOF-based sensor for zinc ion detection in water and blood serum samples. 基于氨基功能化 MOF 的新型传感器,用于检测水和血清样品中的锌离子。
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Pub Date : 2025-02-15 Epub Date: 2024-11-12 DOI: 10.1016/j.saa.2024.125432
Yasmeen A S Hameed, Nada Alkhathami, Razan M Snari, Alaa M Munshi, Omaymah Alaysuy, Muhammad Hadi, Marwah A Alsharif, M A Khalil, Nashwa M El-Metwaly
{"title":"Novel amino-functionalized MOF-based sensor for zinc ion detection in water and blood serum samples.","authors":"Yasmeen A S Hameed, Nada Alkhathami, Razan M Snari, Alaa M Munshi, Omaymah Alaysuy, Muhammad Hadi, Marwah A Alsharif, M A Khalil, Nashwa M El-Metwaly","doi":"10.1016/j.saa.2024.125432","DOIUrl":"10.1016/j.saa.2024.125432","url":null,"abstract":"<p><p>Aquatic systems with low zinc levels can experience a significant decrease in carbon dioxide uptake and limited growth of phytoplankton species. In this study, we describe the use of a new fluorescent sensor based on NH<sub>2</sub>-MIL-53(Al), and modified with glutaraldehyde and sulfadoxine, for selectively detecting zinc ions in water and blood serum samples. Characterization of the synthesized material was performed using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET) surface area analysis, X-ray photoelectron spectroscopy (XPS), and scanning electron microscopy (SEM), confirming successful functionalization and preservation of the MOF structure. The sensor's performance for Zn<sup>2+</sup> detection was evaluated by spectrofluorometry, demonstrating a significant fluorescence enhancement upon Zn<sup>2+</sup> binding due to the interaction between Zn<sup>2+</sup> ions and the sulfonamide groups. With a detection limit as low as 3.14 × 10<sup>-2</sup> ppm, the sensor demonstrates high selectivity for Zn<sup>2+</sup> over other common metal ions. The sensor's response is rapid, stable, and reproducible, making it suitable for practical applications. Real sample analysis was conducted in tap water and blood serum samples, with the results compared to those obtained using ICP-OES and a colorimetric test with 5-bromo-PAPS. The comparison confirmed the high accuracy and reliability of the fluorescent sensor in detecting Zn<sup>2+</sup> ions in complex matrices. NH<sub>2</sub>-MIL-53(Al) modified with glutaraldehyde and sulfadoxine shows potential as a selective fluorescent sensor for Zn<sup>2+</sup> detection, making it a valuable tool for monitoring the environment and biology.</p>","PeriodicalId":94213,"journal":{"name":"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy","volume":"327 ","pages":"125432"},"PeriodicalIF":0.0,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142645355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spectral resolution techniques for the simultaneous spectrophotometric determination of anti-Parkinson drugs in their combined pharmaceutical dosage form and biological sample based on multivariate calibration and absorbance subtraction methods. 基于多元校准和吸光度减法的光谱分辨率技术,用于同时分光光度法测定抗帕金森病药物的药物剂型和生物样品。
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Pub Date : 2025-02-15 Epub Date: 2024-11-12 DOI: 10.1016/j.saa.2024.125399
Fereshteh Zarnooshe Farahani, Mahmoud Reza Sohrabi, Fariba Tadayon
{"title":"Spectral resolution techniques for the simultaneous spectrophotometric determination of anti-Parkinson drugs in their combined pharmaceutical dosage form and biological sample based on multivariate calibration and absorbance subtraction methods.","authors":"Fereshteh Zarnooshe Farahani, Mahmoud Reza Sohrabi, Fariba Tadayon","doi":"10.1016/j.saa.2024.125399","DOIUrl":"10.1016/j.saa.2024.125399","url":null,"abstract":"<p><p>In this study, simultaneous determination of levodopa (LEV) and carbidopa (CBD) in binary mixtures, pharmaceutical formulation, and biological sample was conducted using the application of simple, fast, sensitive, and accurate UV-spectrophotometry in combination with chemometrics methods. The first method is net analyte signal (NAS) based on the multivariate calibration methods. The limit of detection (LOD) and limit of quantification (LOQ) were 0.9758, 0.7633 µg/mL and 2.956, 2.313 µg/mL over the linear range of 5-40 and 0.5-20 µg/mL for LEV and CBD, respectively. In the NAS approach, the mean recovery values of mixtures were 100.12 % for LEV and 99.65 % for CBD, where root mean square error (RMSE) values were 0.0106 and 0.0141 for LEV and CBD, respectively. The second method is absorbance subtraction (AS) based on the absorption factor technique for analyzing the isosbestic point. This model was constructed at an isosbestic point of 261 nm in the range of 5-40 and 0.5-20 µg/mL with coefficient determination (R<sup>2</sup>) of 0.9985 and 0.9996 for LEV and CBD, respectively. AS method could estimate LEV and CBD with LOD values of 1.924 and 0.5657 μg/mL and LOQ values of 5.833 and 1.714 μg/mL, respectively. The recovery percentage was between 91.50 % to 104.60 % with RMSE of 0.1455 for LEV and 92.00 % to 106.66 % with RMSE of 0.2508 for CBD. The introduced approaches have the benefit of concurrent analysis of the mentioned components without any pretreatment. Statistical comparison of the results of real sample analysis with high-performance liquid chromatography (HPLC) did not show a significant difference. These methods can replace HPLC in quality control laboratories when fast, precise, and low-cost analysis is needed.</p>","PeriodicalId":94213,"journal":{"name":"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy","volume":"327 ","pages":"125399"},"PeriodicalIF":0.0,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142645356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Continuous solid-phase extraction spectroscopy and its quantification method for trace analysis. 用于痕量分析的连续固相萃取光谱及其定量方法。
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Pub Date : 2025-02-15 Epub Date: 2024-11-06 DOI: 10.1016/j.saa.2024.125396
Jiayan Shen, Long Li, Kehan Xu, Anqi Jia, Yiping Du, Huipeng Deng
{"title":"Continuous solid-phase extraction spectroscopy and its quantification method for trace analysis.","authors":"Jiayan Shen, Long Li, Kehan Xu, Anqi Jia, Yiping Du, Huipeng Deng","doi":"10.1016/j.saa.2024.125396","DOIUrl":"10.1016/j.saa.2024.125396","url":null,"abstract":"<p><p>This study designed and developed an innovative online detection device based on Continuous Solid-Phase Extraction Spectroscopy (CSPES) for rapid quantitative analysis of environmental water pollutants. The device is highly automated, eliminating environmental interference. Leveraging CSPES technology and adsorption kinetics theory, an online quantitative analysis model between the spectrum and component concentrations was established, along with a concentration calculation method based on the least squares method. The quantitative analysis method was validated using single-component and binary-component sample systems containing Fluoranthene, Benzo[k]Fluoranthene, and Rhodamine 6G. The model exhibited excellent predictive performance, with overall prediction concentration relative errors (RE) ranging from 0.45 % to 8.75 % and relative standard deviations (RSD) of less than 3 %. In real sample applications, recovery rates ranged from 86.8 % to 124.4 %, with RSDs between 0.33 % and 2.22 %. This method provides a robust tool for water quality monitoring and environmental analysis, holding significant potential for application across various fields.</p>","PeriodicalId":94213,"journal":{"name":"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy","volume":"327 ","pages":"125396"},"PeriodicalIF":0.0,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142645352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
New near-infrared fluorescent probe for imaging superoxide anion of cell membrane. 用于细胞膜超氧阴离子成像的新型近红外荧光探针。
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Pub Date : 2025-02-15 Epub Date: 2024-11-12 DOI: 10.1016/j.saa.2024.125431
Bokai Ma, Ziyin Chai, Ya Liu, Zixu He, Xinqi Chen, Chong Qian, Yongjia Chen, Weizhi Wang, Zihui Meng
{"title":"New near-infrared fluorescent probe for imaging superoxide anion of cell membrane.","authors":"Bokai Ma, Ziyin Chai, Ya Liu, Zixu He, Xinqi Chen, Chong Qian, Yongjia Chen, Weizhi Wang, Zihui Meng","doi":"10.1016/j.saa.2024.125431","DOIUrl":"10.1016/j.saa.2024.125431","url":null,"abstract":"<p><p>Selective imaging of superoxide anion is important for understanding its role in cell membrane biology, but is often a challenging task because of the lack of an effective fluorescence probe. In this study, a new near-infrared fluorescent probe (SHX-O) that can target cell membrane was developed for imaging superoxide anion. SHX-O was designed by simultaneously incorporating a sulfonated bis-indole and a diphenylphosphinyl recognition group into the hemicyanine moiety. The probe itself showed a rather weak fluorescence due to the hemicyanine's hydroxyl substitution; however, its reaction with superoxide anion caused a large enhancement of near-infrared fluorescence at 790 nm. Moreover, SHX-O exhibited not only high selectivity for superoxide anion over other reactive oxygen species, but also specific cell membrane localization, which may be attributed to the probe's amphiphilic structure. Using the probe, fluorescence imaging of cell membrane superoxide anion produced in the presence of xanthine oxidase and xanthine has been achieved in living cells. We believe that SHX-O may serve as a potential tool for imaging and investigating superoxide anion of cell membrane.</p>","PeriodicalId":94213,"journal":{"name":"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy","volume":"327 ","pages":"125431"},"PeriodicalIF":0.0,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142645354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Relationship between antioxidant activity and ESIPT process based on flavonoid derivatives: A comprehensive analysis. 基于黄酮类衍生物的抗氧化活性与 ESIPT 工艺之间的关系:综合分析。
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Pub Date : 2025-02-15 Epub Date: 2024-11-02 DOI: 10.1016/j.saa.2024.125370
Xingzhu Tang, Lingling Wang, Yajie Zhang, Chaofan Sun
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