Rejuvenation research最新文献

{"title":"Bushenhuoxue Formula Protects Against Lumbar Facet Joint Osteoarthritis via Acting on TDP-43-Mediated Pyroptosis to Loose Caspase-11/NLRP3 Interaction.","authors":"Biao Zhou, Xu Wei, Xiaolong Lu, Zhiqiang Luo, Can Zhou, Jie Yu, Kai Sun, Liguo Zhu","doi":"10.1177/15491684251382419","DOIUrl":"10.1177/15491684251382419","url":null,"abstract":"<p><strong>Background: </strong>Facet joint osteoarthritis (FJOA) is a serious facet joint cartilage degeneration disorder, which is an important cause of low back pain and disability. Bushenhuoxue formula (BSHXF), a traditional Chinese herbal compound, has been demonstrated to exhibit the ability to improve osteoarthritis. This study aimed to uncover the therapeutic effect and potential mechanism of BSHXF in FJOA.</p><p><strong>Methods: </strong>FJOA rats were treated with BSHXF. Histological staining, immunohistochemistry, TUNEL staining, and flow cytometry were performed for detecting pathological damage, molecule expressions in articular cartilage, chondrocyte apoptosis, and pyroptosis, respectively. Interleukin (IL)-18 and IL-1β levels were quantified with enzyme-linked immunosorbent assay. RT-qPCR and Western blot were carried out for evaluating mRNA and protein expressions. Immunofluorescence and coimmunoprecipitation were used to study the colocalization and interaction between Caspase-11 and Nod-like receptor protein-3 (NLRP3) in chondrocytes.</p><p><strong>Results: </strong>In FJOA rats, BSHXF attenuated articular cartilage pathological damage. Specifically, BSHXF inhibited matrix degradation, as indicated by increasing collagen II and decreasing matrix metalloproteinase 13. BSHXF inhibited articular chondrocyte apoptosis and promoted chondrocyte proliferation. BSHXF decreased Caspase-1 and NLRP3, reduced the rate of Caspase-1/propidium iodide-stained chondrocytes, and inhibited inflammatory cytokines, indicating the attenuated articular chondrocyte pyroptosis. BSHXF downregulated Caspase-11, loosened the interaction between NLRP3 and Caspase-11, as well as upregulated transactive response DNA-binding protein 43 (TDP-43) in FJOA rats. Additionally, in H₂O₂-exposed chondrocytes, TDP-43 overexpression decreased TLR4, p-IκBα, NF-κB P65, and Caspase-11 and declined the co-localization of Caspase-11 and NLRP3. Importantly, TDP-43 knockdown reversed BSHXF-mediated protective effects in FJOA rats.</p><p><strong>Conclusion: </strong>BSHXF inhibited chondrocyte pyroptosis by increasing TDP-43 to protect against FJOA via loosening the interaction of Caspase-11 and NLRP3, which may provide a fundamental basis for treating FJOA cartilage damage.</p>","PeriodicalId":94189,"journal":{"name":"Rejuvenation research","volume":" ","pages":"45-56"},"PeriodicalIF":2.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145497836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine Learning Prediction of Chronic Kidney Disease in Elderly MetS Patients Using NHANES 2011-2020 Data.","authors":"Yu Du, Kai Wang","doi":"10.1177/15491684251399607","DOIUrl":"10.1177/15491684251399607","url":null,"abstract":"<p><strong>Background: </strong>Chronic kidney disease (CKD) is getting more common in elderly people with metabolic syndrome, but early detection and risk prediction are still hard. So we created and validated a CKD risk prediction model tailored for these patients to get past the limits of existing clinical screening tools.</p><p><strong>Methods: </strong>We used data from the National Health and Nutrition Examination Survey and the China Health and Retirement Longitudinal Study to develop and validate the CKD prediction model. We defined CKD based on an estimated glomerular filtration rate < 60 mL/min/1.73 m², calculated with the chronic kidney disease epidemiology collaboration (CKD-EPI) formula. We did feature selection with a mix of strict methods and evaluated model performance across both internal and external validation cohorts. We used 10 machine learning algorithms and four data balancing strategies. The final CKD prediction model had strong predictive power and clinical interpretability for early detection of prevalent CKD.</p><p><strong>Results: </strong>The final model, which uses four key variables (uric acid to high-density lipoprotein cholesterol ratio (UHR), age, uric acid, blood urea nitrogen (BUN)), had predictive accuracies of 0.864 (internal) and 0.831 (external) when using logistic regression. This shows it is robust and can be applied widely. Shapley Additive exPlanations analysis and restricted cubic spline analysis found significant links between the key features and CKD risk.</p><p><strong>Conclusion: </strong>This study developed a reliable, interpretable CKD risk prediction model using routine clinical indicators. It gives an effective tool for large-scale early CKD screening and stratified management in primary care and has high clinical applicability.</p>","PeriodicalId":94189,"journal":{"name":"Rejuvenation research","volume":" ","pages":"62-72"},"PeriodicalIF":2.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145727826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol Inhibits Pancreatic Cancer Progression by Regulating CDC42 Succinylation and Disrupting the Extracellular Matrix.","authors":"Mengde Ding, Qian Wang","doi":"10.1177/15491684251379251","DOIUrl":"10.1177/15491684251379251","url":null,"abstract":"<p><p>Pancreatic cancer (PC) is a highly lethal malignancy with significant drug resistance and recurrence. This study explores the molecular mechanisms by which resveratrol (RES) inhibits PC proliferation, invasion, and migration, aiming to provide new insights for future therapeutic strategies. Network pharmacology was used to construct a component-pathway-target network diagram for the effect of RES on PC. Transwell assays were used to analyze cell invasion. In addition, scratch assays were conducted to evaluate cell migration ability, and qPCR was utilized to monitor the mRNA expression levels of genes. Immunofluorescence and Western blotting were applied to detect protein expression. The results demonstrate that RES exhibits a significant inhibitory effect on PC cells at different concentrations, with 50 μmol/L RES showing the most pronounced inhibition. This observation was further confirmed by Transwell and scratch assays, which showed that RES significantly inhibits PC cell proliferation, invasion, and migration. Network pharmacology analysis suggests that RES may act on the Rap1 pathway to suppress the progression of PC. <i>In vitro</i> experiments confirmed that RES downregulates the expression of CDC42, MMP2, and MMP9. Notably, immunoprecipitation experiments revealed that RES induces the succinylation of CDC42, which, in turn, inhibits the CDC42 signaling pathway. This disruption leads to the destabilization of the extracellular matrix (ECM), thereby impeding tumor progression. This study demonstrates that RES significantly inhibits the proliferation, invasion, and migration of PC cells by mediating the succinylation of CDC42, which in turn inhibits the CDC42 signaling pathway and disrupts the dynamics of the ECM. This mechanism highlights the potential of RES as an anticancer agent and provides new insights for the development of therapeutic strategies for PC.</p>","PeriodicalId":94189,"journal":{"name":"Rejuvenation research","volume":" ","pages":"18-28"},"PeriodicalIF":2.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145082959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical and Physiological Response During Oxidative Stress: A Cross-Species Perspective.","authors":"Dharmsheel Shrivastav, Juhi Mishra, Varun Kumar Sharma, Shilpy Singh, Mohammad Idreesh Khan, Saleh A Alsanie, Fauzia Ashfaq, Mirza Masroor Ali Beg","doi":"10.1177/15491684251386712","DOIUrl":"10.1177/15491684251386712","url":null,"abstract":"<p><p>Oxidative stress, caused by an imbalance between reactive oxygen species (ROS) and antioxidants, is a fundamental challenge affecting both invertebrate and vertebrate organisms. Environmental triggers such as pollutants, heavy metals, pesticides, and seasonal fluctuations, alongside endogenous factors like heme metabolism and disease, contribute to elevated ROS production across animal taxa. These molecular species can damage proteins, membranes, and nucleic acids, compromising cellular integrity and function. The aim of this study is to comprehensively observe and compare the impact of oxidative stress on invertebrates (including protozoa, annelids, arthropods, and mollusks) and vertebrates (fish, amphibians, birds, and mammals), elucidating common and distinct stress pathways. Using integrative evidence from schematic representations and biological pathways, this work highlights conserved mechanisms such as lipid peroxidation, Fenton chemistry, and the upregulation of defense enzymes. Despite evolutionary divergence, the findings demonstrate that oxidative stress responses are universally pivotal in regulating cellular homeostasis, inflammation, and survival. The study underscores the importance of redox balance in animal adaptation and health, offering insight into stress physiology and potential targets for mitigating oxidative damage in diverse species.</p>","PeriodicalId":94189,"journal":{"name":"Rejuvenation research","volume":" ","pages":"29-39"},"PeriodicalIF":2.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145260378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of CD127 on CD28⁺ CD45RA⁺ CD8^br Treg Cells in Mediating the Association Between GDF-15 and Sarcopenia.","authors":"Yangqi Pan, Peng Zheng, Tao Yao, Zi Tao, Luoxiang Fang, Jiafeng Lin","doi":"10.1177/15491684251378963","DOIUrl":"10.1177/15491684251378963","url":null,"abstract":"<p><p>Growth Differentiation Factor 15 (GDF-15) is closely associated with the occurrence and progression of sarcopenia, but the causal relationship between GDF-15 and sarcopenia remains unclear, and it is also uncertain whether immune cells mediate the pathway between GDF-15 and sarcopenia. We employed Mendelian randomization analysis to explore the causal relationship between GDF-15 and sarcopenia, using the inverse variance-weighted (IVW) method as the primary analytical approach, and validated the results through sensitivity analyses. In addition, we explored whether 731 immune cell phenotypes mediate these causal relationships. GDF-15 was negatively correlated with all four traits of sarcopenia, specifically with whole body fat-free mass (odds ratio [OR] = 0.989, 95% confidence interval [CI] = 0.979-0.998, <i>p</i>_IVW = 2.149E-02), left arm fat-free mass (OR = 0.988, 95% CI = 0.979-0.998, <i>p</i>_IVW = 1.345E-02), right arm fat-free mass (OR = 0.987, 95% CI = 0.979-0.995, <i>p</i>_IVW = 1.091E-03), and appendicular lean mass (OR = 0.984, 95% CI = 0.974-0.993, <i>p</i>_IVW = 7.658E-04). Mediation analysis indicated that CD127 on CD28⁺ CD45RA⁺ CD8^br mediated the causal relationship between GDF-15 and sarcopenia, with a mediation proportion of 21.58% (<i>p</i> = 0.023). In conclusion, our study proposed that CD127 on CD28⁺ CD45RA⁺ CD8^br mediates the causal relationship between GDF-15 and sarcopenia, providing potential theoretical support and practical guidance for the innovation of treatment strategies and personalized therapies for sarcopenia.</p>","PeriodicalId":94189,"journal":{"name":"Rejuvenation research","volume":" ","pages":"1-9"},"PeriodicalIF":2.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145076969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of the Geriatric Nutritional Risk Index on Postoperative Cognitive Dysfunction and Complications after Total Hip Arthroplasty Under General Anesthesia: A Retrospective Study.","authors":"Xia Li, Yunyun Sun, Liang Chen, Yuanhai Li","doi":"10.1177/15491684251379234","DOIUrl":"10.1177/15491684251379234","url":null,"abstract":"<p><p>The Geriatric Nutritional Risk Index (GNRI) is widely used to assess nutritional status. However, its association with postoperative cognitive dysfunction (POCD) and postoperative complications in elderly patients receiving total hip arthroplasty remains inadequately explored. GNRI was calculated based on serum albumin and body weight. POCD was diagnosed using the Z-score method based on cognitive test performance on the seventh postoperative day. Glial fibrillary acidic protein (GFAP) and S100β levels were determined using an enzyme-linked immunosorbent assay. A Receiver Operating Characteristic curve was performed to evaluate the predictive value of GNRI. Multivariate logistic regression was conducted to identify risk factors for POCD in elderly patients undergoing total hip arthroplasty. The POCD group was significantly older, had lower educational attainment, longer surgery duration, greater intraoperative blood loss, and lower preoperative GNRI scores compared to the non-POCD group. Preoperative GNRI demonstrated moderate predictive value for POCD, with an area under curve (AUC) of 0.78. Multivariate logistic regression analysis identified age (odds ratio [OR]: 1.214, 95% confidence interval [CI]: 1.047-1.449), blood loss (OR: 1.198, 95% CI: 1.055-1.493), and anesthesia duration (OR: 1.376, 95% CI: 1.112-1.795) as significant risk factors for POCD, while preoperative GNRI (OR: 0.885, 95% CI: 0.768-0.973) was identified as a protective factor. POCD patients exhibited significantly lower Montreal Cognitive Assessment (MoCA) scores and higher serum S100β and GFAP levels than the non-POCD group. Preoperative GNRI was positively correlated with MoCA scores (r = 0.46, <i>p</i> < 0.001) and negatively correlated with serum S100β (r = -0.43, <i>p</i> < 0.001) and GFAP (r = -0.37, <i>p</i> < 0.001) levels. Higher preoperative GNRI scores were associated with a reduced incidence of postoperative complications, including pulmonary infections and liver dysfunction. Preoperative GNRI serves as an effective predictor of POCD and postoperative complications in elderly patients receiving hip arthroplasty.</p>","PeriodicalId":94189,"journal":{"name":"Rejuvenation research","volume":" ","pages":"10-17"},"PeriodicalIF":2.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145115695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Successful Aging and Mortality: Evidence from the Chinese Longitudinal Healthy Longevity Survey (2011-2018).","authors":"Wen Li, Xiangrui Fu, Wenbo Xiao, Guanghan Zhu, Yurong Liu, Xuan Zhang, Wenli Lu, Yuan Wang","doi":"10.1089/rej.2025.0014","DOIUrl":"10.1089/rej.2025.0014","url":null,"abstract":"<p><p>Successful aging is essential for health, particularly as the global population of older adults rises. It assesses whether older adults maintain a healthy lifestyle and enjoy quality in their later years. This study explores the relationship between successful aging and mortality, focusing on how its components relate to mortality across different genders. We conducted a longitudinal analysis of 9633 older adults (aged 65+) from the Chinese Longitudinal Healthy Longevity Survey (2011-2018). This study used two definitions of successful aging: SA I includes five dimensions (the absence of major diseases, no subthreshold depression, freedom from disability, high cognitive function, and active social participation); SA II consists of four dimensions and excludes the absence of major diseases. Both genders exhibited lower mortality rates among those achieving successful aging (males: hazard ratio [HR] = 0.62 for SA I, HR = 0.65 for SA II; females: HR = 0.57 for SA I, HR = 0.58 for SA II). All successful aging dimensions correlated with reduced death risk, except for subthreshold depression in females. In males, significant reductions in mortality were linked to freedom from disability (HR = 0.49) and active social participation (HR = 0.60). In females, these were notable for freedom from disability (HR = 0.46) and high cognitive function (HR = 0.47). SA I, which includes the absence of major diseases, is more relevant for Chinese younger old. The influence of successful aging components on mortality risk varies by gender, emphasizing the importance of addressing functional daily living challenges to enhance the health of older adults in China.</p>","PeriodicalId":94189,"journal":{"name":"Rejuvenation research","volume":" ","pages":"282-290"},"PeriodicalIF":2.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144577483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential Responses of Young and Old Erythrocytes Stored with Vitamin C and Vitamin E in Additive Solution-7.","authors":"Masannagari Pallavi, Vani Rajashekaraiah","doi":"10.1089/rej.2024.0033","DOIUrl":"10.1089/rej.2024.0033","url":null,"abstract":"<p><p>Oxidative stress (OS) causes biochemical and morphological alterations in erythrocytes. The primary factors contributing to OS are aging and storage. Antioxidants significantly alleviate OS. Therefore, this study aimed to investigate the response of young and old erythrocytes to vitamin C and vitamin E during storage. Erythrocytes were separated into young and old by the Percoll method. Each erythrocyte subpopulation was categorized into the i) Control (additive solution-7 [AS-7]) and ii) vitamin C and vitamin E in AS-7 (VC+VE) groups and stored for 21 days at 4°C. OS, antioxidant, and aging markers were analyzed on days 1, 14, and 21. The activity of antioxidant enzymes was similar throughout storage in young cells. However, superoxide dismutase activity elevated in old cells (Control and VC+VE) on days 1 and 21. Catalase (CAT) activity increased on days 14 and 21, whereas glutathione peroxidase (GPX) increased on days 1 and 14 in old Controls. However, in old VC+VE, CAT increased on day 21 and GPX increased on day 1. Advanced oxidation protein products, superoxides, glutathione, and uric acid increased in old cells throughout storage. Malondialdehyde decreased in old VC+VE compared with old Control on days 14 and 21. Sialic acids and glutamate oxaloacetate transaminase activity were higher in young cells compared to old cells. Young cells exhibited lower oxidative changes throughout storage. Vitamin C and vitamin E were effective in maintaining the redox balance in old cells. These findings emphasize the need for specific approaches for different subpopulations during erythrocyte banking.</p>","PeriodicalId":94189,"journal":{"name":"Rejuvenation research","volume":" ","pages":"272-281"},"PeriodicalIF":2.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141422367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of COVID-19 Isolation on Sarcopenia in Older Adults: A Cohort Study from Taiwan.","authors":"Pu-Jun Fang, Ping-Hsuan Kuo, Tung-Wei Kao, Tao-Chun Peng","doi":"10.1177/15491684251381561","DOIUrl":"10.1177/15491684251381561","url":null,"abstract":"<p><p>Previous studies on sarcopenia and COVID-19 have primarily focused on pathophysiological mechanisms, leaving the effects of social isolation policies largely unexplored. Taiwan offers a unique environment to investigate this issue. This study investigates the association between COVID-19-related isolation and sarcopenia in older adults in Taiwan. Participants aged 65 and older were enrolled from annual geriatric health check-ups conducted between 2018 and 2023. The years from 2018 to 2020 were designated as the pre-COVID-19 period, while 2022-2023 was considered the post-COVID-19 era. Demographic data and health conditions were collected. The assessments encompassed body composition, muscle strength, and physical performance. The prevalence and trends of sarcopenia were analyzed. The prevalence of low muscle strength declined in 2021 but subsequently increased from 2022 to 2023 (<i>p</i>_quadratic = 0.018). In 2023, compared with 2018-2020, the odds ratio for sarcopenia and severe sarcopenia was 1.3 (95% confidence interval [CI] 1.01-1.67), and the odds ratio for low physical performance was 1.73 (95% CI 1.39-2.15). This is the first report describing the association between COVID-19 pandemic-related policies and the prevalence of sarcopenia, revealing a significant increase in sarcopenia prevalence following the pandemic. Our results emphasize the necessity of tailored strategies to support older adults during and after health crises.</p>","PeriodicalId":94189,"journal":{"name":"Rejuvenation research","volume":" ","pages":"263-271"},"PeriodicalIF":2.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145093309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BICDL1 Mediates Pyroptosis to Promote Breast Cancer Cell Proliferation, Inhibition, and Migration.","authors":"Xu Chu, Ling Xiang, Yan Zou, Xiang Li, Na Zhang, Hao Zhao","doi":"10.1177/15491684251375414","DOIUrl":"10.1177/15491684251375414","url":null,"abstract":"<p><p>Elevated levels of Bicaudal-D Family Like Cargo Adaptor 1 (BICDL1) are associated with poor prognosis in various cancers. However, the role of BICDL1 in breast cancer (BC) has not been reported. We analyzed BICDL1 expression in BC using bioinformatics and molecular experiments. Gene Set Enrichment Analysis was conducted to identify significantly enriched signaling pathways related to BICDL1. The effects of BICDL1 on BC cell proliferation, migration, invasion, and expression of epithelial-mesenchymal transition (EMT)-related proteins were assessed using colony formation assays, Transwell assays, and Western blot. The influence of BICDL1 on pyroptosis in BC cells was determined by measuring the expression of key pyroptosis proteins (NLRP3, GSDMD-N, procaspase-1, cleaved caspase-1, ASC), lactate dehydrogenase release, and cytokines (IL-1β and IL-18). We also investigated whether BICDL1 promotes BC proliferation and metastasis by regulating pyroptosis using Nigericin, an agent that induces NLRP3 inflammasome activation. The results showed that BICDL1 was significantly overexpressed in BC tissues and cells. Knockdown of BICDL1 inhibited BC cell proliferation, migration, invasion, and the EMT process, while overexpression of BICDL1 had the opposite effects. BICDL1 was enriched in the pyroptosis pathway; overexpression of BICDL1 suppressed pyroptosis, thus promoting BC cell proliferation, inhibition, and migration. Notably, the addition of Nigericin reversed the effects of BICDL1 overexpression on BC cells. These results suggested that BICDL1 promoted BC cell proliferation, inhibition, and migration by hindering pyroptosis. These findings indicate that BICDL1 is a potential biomarker for BC treatment.</p>","PeriodicalId":94189,"journal":{"name":"Rejuvenation research","volume":" ","pages":"300-307"},"PeriodicalIF":2.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145056456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}