Polish journal of veterinary sciences最新文献

Prevalence of subclinical mastitis in sheep, etiological agents, and antimicrobial susceptibility in Northern Cyprus. 北塞浦路斯绵羊亚临床乳腺炎的患病率、病因和抗菌药物敏感性。

Polish journal of veterinary sciences Pub Date : 2025-06-01 DOI: 10.24425/pjvs.2025.154939

O Ergene, H Baloglu, V Haciogullari, H E Çolakoğlu

{"title":"Prevalence of subclinical mastitis in sheep, etiological agents, and antimicrobial susceptibility in Northern Cyprus.","authors":"O Ergene, H Baloglu, V Haciogullari, H E Çolakoğlu","doi":"10.24425/pjvs.2025.154939","DOIUrl":"https://doi.org/10.24425/pjvs.2025.154939","url":null,"abstract":"In Northern Cyprus, around 51% of halloumi cheese is produced from sheep milk, and therefore the livelihood of the farmers mostly depends on the sheep milk production. However mastitis, an inflammation of the udder, significantly affects this production. Due to a lack of sufficient data concerning the prevalence, etiology, and antimicrobial resistance of sheep mastitis, there remains no effective method to control the disease. This study aims to estimate the prevalence of subclinical mastitis (SCM) and identify bacterial etiological agents and the antimicrobial susceptibility profiles of bacterial isolates in sheep in Northern Cyprus. A total of 227 milk samples taken from sheep were analysed using somatic cell count (SCC), bacteriological isolation-identification, and antimicrobial susceptibility procedures. Pathogens were isolated in 62 (27.3%) sheep milk samples. Somatic cell counts of more than 500000 cells/ml were found in 56 (24.6%). S. aureus (12.8%) was the most common isolate from the milk samples, followed by NAS (non-Aureus staphylococci) species (11.9%), Escherichia coli (0.9%), Streptococci (0.4%), Bacillus spp. (0.9%) and Staph spp. (0.4%). While a high resistance to sulphamethaxazole/trimetoprim (81.5%) was found, no resistance to gentamicin (10.6%) was found. The study findings indicate that subclinical mastitis is a serious problem in Cyprus. Therefore, continuous observation of subclinical mastitis and application of antibiogram tests to combat mastitis and antibiotic resistance and reduce economic losses are needed.","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"28 2","pages":"203-211"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of knockdown resistance mutations in Ctenocephalides felis samples from the cat populations of Istanbul Province. 伊斯坦布尔省猫种群中猫头蠓耐敲低突变的调查。

Polish journal of veterinary sciences Pub Date : 2025-06-01 DOI: 10.24425/pjvs.2025.154938

T Kuntuz, Y Gunes, B Diren Sigirci, C Anlas, A B Sari, T Bakirel, O Ustuner

{"title":"Investigation of knockdown resistance mutations in Ctenocephalides felis samples from the cat populations of Istanbul Province.","authors":"T Kuntuz, Y Gunes, B Diren Sigirci, C Anlas, A B Sari, T Bakirel, O Ustuner","doi":"10.24425/pjvs.2025.154938","DOIUrl":"https://doi.org/10.24425/pjvs.2025.154938","url":null,"abstract":"Effective control of the cat flea (Ctenocephalides felis) infestation is essential for human and animal health. To date, several ectoparasitic drugs, such as carbamates, neonicotinoids, phenylpyrazoles, and pyrethroids have been used to control flea infestations; however their widespread use has led to resistance, hampering treatment success. In this study, the resistance potential of C. felis collected from cats to fipronil (FIP), imidacloprid (IMI), flumethrin (FLU), and propoxur (PRO), which are the commonly used compounds for flea control, was investigated by molecular analyses. Bioassays encompassed exposure of adult cat fleas to FIP (2%), IMI (6%), FLU (0.1%), and PRO (0.1%)-impregnated papers using an insecticide susceptibility test kit according to the WHO protocol. Afterward, PCR was performed to identify mutations indicating resistance to FIP and FLU. Based on molecular analyses, mutations in the para gene of C. felis were identified as L1014F and T929V, indicating FLU resistance. Frequencies of T929V and L1014F mutations in fleas were 2.5% and 10%, respectively. On the other hand, two fleas that survived after eight hours of exposure to FIP lacked the A302S mutation, which was partially associated with FIP resistance. Even though our results revealed no resistance to FIP, IMI, and PRO in fleas collected from the study's cat population, the occurrence of FLU resistance due to mutations in the para gene of the fleas was demonstrated at the molecular level.","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"28 2","pages":"195-202"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of potential clinical approaches for the expression of prolactin receptor (PRL-R) and vascular endothelial growth factor (VEGF) in various feline mammary gland tumors. 各种猫科乳腺肿瘤中催乳素受体（PRL-R）和血管内皮生长因子（VEGF）表达的潜在临床途径评估

Polish journal of veterinary sciences Pub Date : 2025-06-01 DOI: 10.24425/pjvs.2025.154946

S Giziński, Ł Zdrojkowski, J Olszewski, K Malin, A Niwińska, E Kautz-Wasilewska, A Rodo, A Jaeckel, M Domino

{"title":"Assessment of potential clinical approaches for the expression of prolactin receptor (PRL-R) and vascular endothelial growth factor (VEGF) in various feline mammary gland tumors.","authors":"S Giziński, Ł Zdrojkowski, J Olszewski, K Malin, A Niwińska, E Kautz-Wasilewska, A Rodo, A Jaeckel, M Domino","doi":"10.24425/pjvs.2025.154946","DOIUrl":"https://doi.org/10.24425/pjvs.2025.154946","url":null,"abstract":"Feline mammary gland tumors are a serious health concern, resulting in a significant reduction in the animal's lifespan and a decrease in the overall quality of life. Malignant tumors often lead to recurrences and metastases. Among endogenous factors that may influence the development or progression of mammary neoplasia, prolactin (PRL) and vascular endothelial growth factor (VEGF) appear to be of crucial importance. This study involved 60 queens with surgically removed mammary gland tumors, which were subsequently stained with hematoxylin and eosin (HE) and immunofluorescence to assess the expression of PRL and VEGF. Variables considered during analyses included the time of ovariohysterectomy, inflammation severity and clinical tumor behavior. The VEGF expression in tumors exhibited an increase in malignant cases, providing evidence of heightened angiogenesis. A lack of differences in the overall expression of PRL receptor was found between tumor types. However, the lower expression of PRL receptor in tumors with increased inflammation may suggest PRL's immunomodulating functions in feline malignant neoplastic tumors. Interestingly, the absence of positive influence of gonadectomy on tumor behavior highlights the need for further research regarding this form of prevention. High expression of PRL receptor and VEGF only in distant metastases may prompt future research on the proangiogenic function of PRL in feline mammary gland tumors.","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"28 2","pages":"271-282"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single or combined use of intermittent fasting and probiotics reduce Campylobacter colonization in the murine gut. 单独或联合使用间歇性禁食和益生菌可减少弯曲杆菌在小鼠肠道中的定植。

Polish journal of veterinary sciences Pub Date : 2025-06-01 DOI: 10.24425/pjvs.2025.154942

M N Açık, Ü G Doğu, A Geyik, M Girgin, A Ulucan, B Karagülle, B Çetinkaya

{"title":"Single or combined use of intermittent fasting and probiotics reduce Campylobacter colonization in the murine gut.","authors":"M N Açık, Ü G Doğu, A Geyik, M Girgin, A Ulucan, B Karagülle, B Çetinkaya","doi":"10.24425/pjvs.2025.154942","DOIUrl":"https://doi.org/10.24425/pjvs.2025.154942","url":null,"abstract":"The objective of the present study was to investigate the impact of single or combined use of intermittent fasting and probiotics, which have been recommended as an alternative to antibiotics in recent years due to their proven efficacy, against Campylobacter colonization in mice. For this purpose, mice infected with Campylobacter jejuni were divided into groups and exposed to intermittent fasting and probiotics, alone and together. At the end of the experimental study, ileum and cecum contents were obtained for microbiological analyzes, and stomach and intestinal tissue samples were collected for histopathological analyzes. It was determined that the level of C. jejuni colonization in the ileum and cecum of mice in the positive control group (PC group) was significantly higher than in the other groups (p<0.05). It was also determined that the level of C. jejuni colonization in the ileum of mice in the probiotic and intermittent fasting group (PB +IF group) was lower than in the other groups and the difference was statistically significant (p<0.05). As a result of the histological analyzes, mild inflammatory reaction was observed to occur in the stomach and intestinal tissues of the animals in the experimental groups, and the severity of the inflammation was lower in the PB +IF group than in the other groups. The findings of this study indicate that single or combined use of intermittent fasting and probiotics may represent a safe and feasible strategy for the control of Campylobacter infections.","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"28 2","pages":"233-241"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimation of heritability and genetic trend for herd life and productive life in Organic Holstein Cattle. 有机荷斯坦牛群体生活和生产生活的遗传力和遗传趋势估计。

Polish journal of veterinary sciences Pub Date : 2025-06-01 DOI: 10.24425/pjvs.2025.154947

S C Baycan, S Duru

{"title":"Estimation of heritability and genetic trend for herd life and productive life in Organic Holstein Cattle.","authors":"S C Baycan, S Duru","doi":"10.24425/pjvs.2025.154947","DOIUrl":"https://doi.org/10.24425/pjvs.2025.154947","url":null,"abstract":"This study aims to estimate the heritability and genetic trend for herd life (HL) and productive life (PL) on an organic dairy cattle farm in the Aegean Region of Turkey. The data from 1093 cows culled from the farm were evaluated. Ancestors were collected by going 6 generations back. Thus, the pedigree file consisted of 4212 animals. Variance components and breeding values were estimated using the MTDFREML package program. The heritability for both HL and PL was found to be equal and 0.16. Standard errors of the heritabilities were 0.06 and 0.08, respectively. Genetic progress per year for HL and PL was 1.60 and 0.60 days, respectively. Estimations for HL and PL were generally consistent with other studies. Heritabilities indicate that breeding for HL and PL is possible; however, response to selection would be slow. Despite the extended length of the selection process, genetic variation could be sufficient for a successful breeding. Indirect selection based on particular traits that have a strong relationship with HL and PL may be beneficial. Our study showed a phenotypic decline in HL and PL, confirming the fluctuation in genetic trends. Therefore, the importance of life traits in the selection index should be emphasised.","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"28 2","pages":"283-289"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report on the genomic characterization of Teschovirus B3 in Jiangxi Province, China. 中国江西省乙型肝炎病毒基因组特征的首次报道。

Polish journal of veterinary sciences Pub Date : 2025-06-01 DOI: 10.24425/pjvs.2025.154950

W Liu, D Zhao, Y Peng, Z Zhou, X Tang, T Yang

引用次数: 0

Effects of coadministration of foot and mouth disease vaccine and inactivated parapoxvirus ovis on humoral immunity in cattle. 口蹄疫疫苗与灭活禽副痘病毒联合接种对牛体液免疫的影响。

Polish journal of veterinary sciences Pub Date : 2025-06-01 DOI: 10.24425/pjvs.2025.154941

R Canbar, M Uslu

{"title":"Effects of coadministration of foot and mouth disease vaccine and inactivated parapoxvirus ovis on humoral immunity in cattle.","authors":"R Canbar, M Uslu","doi":"10.24425/pjvs.2025.154941","DOIUrl":"10.24425/pjvs.2025.154941","url":null,"abstract":"Foot and mouth disease (FMD) is an acute viral disease in animals. Inactive parapoxvirus ovis (IPPVO) strengthens humoral immunity. This study aimed to determine the effect of IPPVO application together with FMD vaccine on cattle immunity. It included 30 Holstein cattle randomly divided into two groups: one was administered only the FMD vaccine, and the other was administered the FMD vaccine and IPPVO simultaneously. Control blood was collected from all animals at 0 hours. Serum TNF-α, IL-1β, and IL-6 levels were measured in blood samples collected at 4, 8, 12, 16, and 24 hours post-vaccination, while serum IgG and IgM levels were measured in blood samples collected at 4, 8, 12, 16, and 20 days post-vaccination using ELISA kits. While no changes in serum TNF-α, IL-1β, and IL-6 levels were detected in the FMD group, IL-1β levels significantly increased (peaking at four hours) in the FMD + IPPVO group. In the FMD group, while IgG levels increased significantly (peaking at 16 days), IgM levels did not change. In the FMD + IPPVO group, IgG level on day 8 was higher than the 16 days value. Also, the IgM level increased significantly on day 16. In conclusion, the application of FMD with IPPVO increases the primary immune response (IgM), but it does not affect the long-term immune response (IgG).","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"28 2","pages":"225-231"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial flora and antibiogram sensitivity in the preputium samples of healthy rams. 健康公羊包皮菌群及抗生素敏感性研究。

Polish journal of veterinary sciences Pub Date : 2025-06-01 DOI: 10.24425/pjvs.2025.154943

S Konak, F Avdatek

{"title":"Bacterial flora and antibiogram sensitivity in the preputium samples of healthy rams.","authors":"S Konak, F Avdatek","doi":"10.24425/pjvs.2025.154943","DOIUrl":"https://doi.org/10.24425/pjvs.2025.154943","url":null,"abstract":"This study was conducted to determine the presence of bacterial flora in the preputium samples of healthy rams in the province of Afyonkarahisar and identify the antibiotic sensitivity of the isolates. Preputial swab samples were collected from a total of 50 healthy 2-3-year-old rams in the center, districts, and villages of Afyonkarahisar, including 20 Merino, 10 Hampshire, and 20 Pirlak breed rams. Seventy-eight isolates obtained from the 50 clinically healthy rams were identified using standard microbiological and biochemical methods, as well as the VITEK-2 automated system device. Forty-four isolates were Gram-positive bacteria (56.4%), 29 isolates were Gram-negative bacteria (37.2%), and 5 isolates were yeasts (6.4%). Seventeen genera consisting of Acinetobacter lwoffii, Aerococcus viridans, Aeromonas hydrophila, Bacillus spp., Candida spp., Escherichia coli, Erysipelothrix rhusiopathiae, Kocuria kristinae, Kocuria rosea, Kytococcus sedentarius, Lactococcus lactis, Mannheimia haemolytica, Neisseria animaloris, Salmonella enterica ssp. diarizonae, Sphingobacterium thalpophilum, Sphingomonas paucimobilis, Staphylococcus capitis, Staphylococcus cohnii spp. urealyticus, Staphylococcus epidermidis, Staphylococcus lentus, Staphylococcus xylosus, Streptococcus ovis, and Streptococcus thoraltensis were isolated. The most frequently isolated species in the tested animals were Staphylococcus spp. (25.6%), E. coli (21.8%), Streptococcus spp. (7.7%), A. viridans (6.4%), Lactococcus lactis ssp. (6.4%), and Candida spp. (6.4%). The susceptibility of the isolates to ampicillin, enrofloxacin, erythromycin, florfenicol, gentamicin, tetracycline, ceftiofur, and trimethoprim-sulfamethoxazole was tested using the Kirby-Bauer disk diffusion method (Bauer et al. 1966). Most isolates were susceptible to ceftiofur, enrofloxacin, gentamicin, and florfenicol, while most were resistant to erythromycin, tetracycline, and ampicillin.","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"28 2","pages":"243-250"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of A2 allele frequency in Taiwanese Holstein cattle using genetic testing. 台湾荷斯坦牛A2等位基因频率的基因检测研究。

Polish journal of veterinary sciences Pub Date : 2025-06-01 DOI: 10.24425/pjvs.2025.154951

C H Chao, J W Shiau

{"title":"Investigation of A2 allele frequency in Taiwanese Holstein cattle using genetic testing.","authors":"C H Chao, J W Shiau","doi":"10.24425/pjvs.2025.154951","DOIUrl":"https://doi.org/10.24425/pjvs.2025.154951","url":null,"abstract":"β-Casein accounts for approximately 30% of total milk protein, with the A1 and A2 variants being the most common. A1 β-casein may release β-casomorphin-7 (BCM-7) during digestion, which is associated with adverse health effects, whereas A2 β-casein does not. This study investigated the A2 allele frequency and its potential influence on milk performance in Holstein cattle in Taiwan. A total of 1,050 cows from five herds were genotyped using the GeneSeek 50K SNP chip. The A2 allele frequency ranged from 0.58 to 0.75 among herds, with an average of 0.66. Hardy-Weinberg equilibrium tests indicated no significant deviation within herds. Pedigree validation confirmed Mendelian inheritance of A1 and A2 alleles. Furthermore, 348 cows with complete lactation records were evaluated for 305-day mature equivalent milk and fat yields across genotypes. Although cows with the A1/A1 genotype showed numerically higher milk and fat yields, one-way ANOVA and Tukey's HSD tests revealed no statistically significant differences. These results suggest that while A2 allele selection is increasing in Taiwan, β-casein genotype does not significantly influence milk or fat production in the studied population.","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"28 2","pages":"303-309"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic disorders in cows and the level of immunity in calves. 奶牛代谢紊乱和小牛免疫水平。

Polish journal of veterinary sciences Pub Date : 2025-06-01 DOI: 10.24425/pjvs.2025.154953

R Urban-Chmiel, P Sobiech

引用次数: 0