Laboratory medicine最新文献_第5页

Development and validation of a prediction model for gastric cancer: a single-center prospective study. 胃癌预测模型的开发与验证：一项单中心前瞻性研究。

Laboratory medicine Pub Date : 2025-01-06 DOI: 10.1093/labmed/lmae060

Suyu Sun, Feifei Huang, Xueqin Xu, Ke Xu, Tingting Peng, Wenjing Bai, Chunwei Huang, Xingzhong Hu, Yong Pan

{"title":"Development and validation of a prediction model for gastric cancer: a single-center prospective study.","authors":"Suyu Sun, Feifei Huang, Xueqin Xu, Ke Xu, Tingting Peng, Wenjing Bai, Chunwei Huang, Xingzhong Hu, Yong Pan","doi":"10.1093/labmed/lmae060","DOIUrl":"10.1093/labmed/lmae060","url":null,"abstract":"Objective: This study aimed to develop and validate a novel nomogram for diagnosing gastric cancer (GC).Methods: In this prospective analysis, 146 patients of Wenzhou Central Hospital were recruited for a GC group and a benign lesion group and were divided into a training set and an internal validation set in a ratio of 7:3. Clinical and analytical characteristics were collected and analyzed by logistic regression analysis. The performance of the predictive model was evaluated using the receiver operating characteristic curve, calibration curve, and decision curve analysis.Results: There were 5 variables, namely albumin, carcinoembryonic antigen, carbohydrate antigen 125, creatinine, and small proline-rich protein 2A, that were identified as the final parameters for the developed model. In the training and internal validation sets, the area under the curve of the model was 0.968 and 0.979, respectively, showing good diagnostic performance.Conclusion: This study developed and validated a new nomogram based on 5 parameters. This model shows good diagnostic performance in distinguishing GC from benign lesion groups and has certain significance in clinical application.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"31-36"},"PeriodicalIF":0.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142407342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Navigating the conundrum of co-existing autoantibodies and alloantibodies in a case of Evans syndrome. 解决埃文斯综合征病例中同时存在的自身抗体和同种抗体的难题。

Laboratory medicine Pub Date : 2025-01-06 DOI: 10.1093/labmed/lmae051

Pallabi Dash, Nilasish Pani, Jayant Kumar Panda, Smita Mahapatra, Binay Bhusan Sahoo, Tusharkantee Behera

引用次数: 0

Common RBC antigens in O type Tunisian blood donors and their importance in alloimmunization. O 型突尼斯献血者中常见的红细胞抗原及其在同种免疫中的重要性。

Laboratory medicine Pub Date : 2025-01-06 DOI: 10.1093/labmed/lmae062

Mohamed Hichem Sellami, Wafa Aïssa, Hamida Ferchichi, Eya Ghazouani, Manel Châabane, Houda Kâabi, Slama Hmida

{"title":"Common RBC antigens in O type Tunisian blood donors and their importance in alloimmunization.","authors":"Mohamed Hichem Sellami, Wafa Aïssa, Hamida Ferchichi, Eya Ghazouani, Manel Châabane, Houda Kâabi, Slama Hmida","doi":"10.1093/labmed/lmae062","DOIUrl":"10.1093/labmed/lmae062","url":null,"abstract":"Background: The presence of some red blood cell (RBC) antigens may affect the preference for using type O blood in emergency situations because they may induce complex or multiple alloimmunization in special circumstances.Methods: A subgroup of 77 type O blood Tunisian donors were genotyped for 19 common blood alleles using the single specific primer-polymerase chain reaction method. The statistical analysis was done using HaploView software.Results: The study showed the dominance of the alleles RH*5, KEL*2, FY*2, and CO*1 and the absence of the homozygous state of the KEL*1 and CO*2 alleles. Furthermore, a complete linkage disequilibrium between the RH*2/RH*4 and RH*3/RH*5 loci and the FY*Null/FY*Exp and FY*A/FY*B loci was detected. Additionally, it seems that sensitization to MNS:3, FY:1, and RH:3 may constitute a potential factor for alloimmunization after transfusion with O blood type units: the probabilities of simple alloimmunizations are 24.5 per 100, 18.5 per 100, and 18 per 100, respectively. Multiple alloimmunization against RH:1;KEL:1 or RH:1;KEL:1;RH:3 phenotypes may occur, with probabilities of 7 per 1000 and 2 per 1000, respectively.Conclusion: Some O-type RBC units may contain blood with very immunogenic phenotypes, the use of which in an emergency requires great caution because it can be a step towards subsequent alloimmunization.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"44-48"},"PeriodicalIF":0.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141908744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LEP rs7799039 and LEPR rs1137101 gene variants are not associated with clinical features in patients with metabolic syndrome in the Turkish population. LEP rs7799039 和 LEPR rs1137101 基因变异与土耳其人群中代谢综合征患者的临床特征无关。

Laboratory medicine Pub Date : 2025-01-06 DOI: 10.1093/labmed/lmae061

Marjan Jabbarli, Naci Senkal, Fatima Ceren Tuncel, Yasemin Oyaci, Merve Guzel Dirim, Murat Kose, Sacide Pehlivan, Alpay Medetalibeyoglu

{"title":"LEP rs7799039 and LEPR rs1137101 gene variants are not associated with clinical features in patients with metabolic syndrome in the Turkish population.","authors":"Marjan Jabbarli, Naci Senkal, Fatima Ceren Tuncel, Yasemin Oyaci, Merve Guzel Dirim, Murat Kose, Sacide Pehlivan, Alpay Medetalibeyoglu","doi":"10.1093/labmed/lmae061","DOIUrl":"10.1093/labmed/lmae061","url":null,"abstract":"Objectives: Genetic predisposition plays a role in the etiology of metabolic syndrome (MetS), an important health problem worldwide. Leptin (LEP), produced by adipose tissue, plays a crucial role in the development of MetS. In this study, we evaluated the effects of LEP and LEP receptor (LEPR) variants on clinical findings and risk of developing MetS in the Turkish population.Methods: A total of 320 patients were included in the study, of whom 150 were patients with MetS and 170 were healthy controls. DNA was extracted from blood samples. LEP rs7799039 and LEPR rs1137101 variants were genotyped using the polymerase chain reaction-based restriction fragment length polymorphism method. The genotype distributions of these variants and clinical and laboratory findings were compared.Results: The LEP rs7799039 GA and AA genotypes and A allele frequencies were higher in participants with MetS than in the control group. For LEP rs7799039, the genotype AA-GA was higher in males, and the GG genotype was higher in females. On analyzing the clinical outcomes associated with these variants, it was observed that individuals possessing LEP rs7799039 GA and AA genotypes displayed elevated levels of triglycerides. In addition, those with the AG-GG genotype of LEPR rs1137101 had lower mean hemoglobin levels.Conclusion: Our results showed that the LEP rs7799039 and LEPR rs1137101 variants may be associated with both the risk of MetS development and clinical findings. Among the various contributors to MetS, a genetic predisposition is commonly recognized as the primary cause.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"37-43"},"PeriodicalIF":0.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141972503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic plasma exchange for hyperviscosity syndrome in IgA multiple myeloma. 治疗性血浆置换用于治疗 IgA 多发性骨髓瘤的高粘滞性综合征。

Laboratory medicine Pub Date : 2025-01-06 DOI: 10.1093/labmed/lmae054

Nalan Yurtsever, Thomas C Binns, Jeanne E Hendrickson, Christopher A Tormey, Edward S Lee

引用次数: 0

Evaluation of polypropylene CSF low-bind collection tubes for trace metal contamination. 评估聚丙烯 CSF 低粘合剂收集管的痕量金属污染。

Laboratory medicine Pub Date : 2025-01-06 DOI: 10.1093/labmed/lmae067

Anna C Bitzer, Paul J Jannetto, Joshua A Bornhorst

{"title":"Evaluation of polypropylene CSF low-bind collection tubes for trace metal contamination.","authors":"Anna C Bitzer, Paul J Jannetto, Joshua A Bornhorst","doi":"10.1093/labmed/lmae067","DOIUrl":"10.1093/labmed/lmae067","url":null,"abstract":"Background: Due to the ability of metal ions to cross the blood-brain barrier, there has been interest in analyzing cerebrospinal fluid (CSF) for trace element concentrations to investigate possible correlations with neurodegenerative diseases. In this study, Sarstedt polypropylene CSF collection tubes were analyzed to determine the contamination levels of aluminum, titanium, chromium, manganese, cobalt, nickel, molybdenum, gadolinium, vanadium, arsenic, cadmium, mercury, lead, thallium, selenium, copper, zinc, and iron.Methods: Sarstedt polypropylene CSF collection tubes from 2 separate lots (n = 10 per lot) were filled with a 2 mL aliquot of a CSF pool with known element concentrations. After 24 hours of leaching at room temperature, all 18 elements were analyzed via inductively coupled plasma mass spectrometry (ICP-MS). Results were subtracted from the initial pool concentration to determine contamination levels.Results: No detectable contamination above the assay limit of detection was found in 11 analytes. Molybdenum and selenium contamination was measured in all tubes, and aluminum, titanium, manganese, thallium, and zinc had minimal levels of sporadic detectable contamination in 25% or fewer of the tubes tested.Conclusions: Sarstedt polypropylene CSF tubes are an acceptable collection tube for the analysis of most assessed metals in CSF.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":"64-69"},"PeriodicalIF":0.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141972502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: The utility of an algorithm based on procalcitonin monitoring in patients with sepsis. 更正：基于脓毒症患者降钙素原监测的算法的实用性。

Laboratory medicine Pub Date : 2025-01-06 DOI: 10.1093/labmed/lmae093

引用次数: 0

Case report - Hyperthermia and nuclear hypersegmentation of blood cells. 病例报告-热疗和核细胞超分割。

Laboratory medicine Pub Date : 2025-01-03 DOI: 10.1093/labmed/lmae105

C Rivière, S Vimeux, S Laurens, L Deluche, P Y Juvin, A Sanson, D Metsu

引用次数: 0

Diagnostic value of TAP, PIVKA-II, and AFP in hepatocellular carcinoma and their prognostic value for patients treated with transarterial chemoembolization. TAP、PIVKA-II、AFP对肝细胞癌的诊断价值及其对经动脉化疗栓塞患者的预后价值。

Laboratory medicine Pub Date : 2025-01-02 DOI: 10.1093/labmed/lmae104

Delu Gan, Yali Wang, Xin Yang, Juan Huang, Lijun Zhang, Bianqin Guo, Pu Li, Dan Gou

{"title":"Diagnostic value of TAP, PIVKA-II, and AFP in hepatocellular carcinoma and their prognostic value for patients treated with transarterial chemoembolization.","authors":"Delu Gan, Yali Wang, Xin Yang, Juan Huang, Lijun Zhang, Bianqin Guo, Pu Li, Dan Gou","doi":"10.1093/labmed/lmae104","DOIUrl":"https://doi.org/10.1093/labmed/lmae104","url":null,"abstract":"Objective: The diagnosis and prognosis of hepatocellular carcinoma (HCC) present significant challenges in clinical practice. This study aimed to evaluate the clinical utility of tumor abnormal protein (TAP), Prothrombin induced by vitamin K absence-II (PIVKA-II), and alpha-fetoprotein (AFP) in diagnosing HCC as well as to investigate their prognostic significance in patients with HCC undergoing transarterial chemoembolization.Methods: A total of 93 HCC patients were enrolled and 101 healthy individuals served as controls. Fresh venous blood samples were collected, and TAP, PIVKA-II, and AFP levels were measured by chemiluminescence immunoassay.Results: Significant differences in TAP, PIVKA-II, and AFP levels were found between HCC patients and healthy individuals. The combined assay of TAP, AFP, and PIVKA-II showed better diagnostic performance for HCC. Patients who underwent transarterial chemoembolization and achieved complete response (CR) had lower levels of prechemotherapy serum TAP, AFP, and PIVKA-II. There are significant differences in levels of TAP, AFP, and PIVKA-II between CR and partial response (PR), CR and stable disease (SD), and CR and progressive disease (PD).Conclusion: Combined detection of TAP, PIVKA-II, and AFP has better diagnostic performance for HCC. Higher levels of prechemotherapy serum TAP, AFP, and PIVKA-II are significantly associated with poor clinical chemoresponse.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142924363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CALR frameshift mutation detection in myeloproliferative neoplasms by microfluidic chip analysis. 应用微流控芯片检测骨髓增殖性肿瘤CALR移码突变。

Laboratory medicine Pub Date : 2024-12-24 DOI: 10.1093/labmed/lmae096

Michael P Greenwood, Keith M Newton, Kristi L Pepper, Heather L Hendrickson, Randall J Olsen, Jessica S Thomas

{"title":"CALR frameshift mutation detection in myeloproliferative neoplasms by microfluidic chip analysis.","authors":"Michael P Greenwood, Keith M Newton, Kristi L Pepper, Heather L Hendrickson, Randall J Olsen, Jessica S Thomas","doi":"10.1093/labmed/lmae096","DOIUrl":"https://doi.org/10.1093/labmed/lmae096","url":null,"abstract":"Background: CALR mutation analysis is routinely used to diagnose BCR/ABL1-negative myeloproliferative neoplasms. The 2 most common CALR mutations are a 52-base pair (bp) deletion and a 5-bp insertion, which account for approximately 85% of cases.Methods: To evaluate our new microfluidic chip assay, we tested CALR mutant and wild-type specimens that were previously analyzed using conventional methods at a reference laboratory. Samples included EDTA-anticoagulated peripheral blood and bone marrow specimens, air dried bone marrow aspirate smears, and formalin-fixed, paraffin-embedded bone marrow sections. CALR exon 9 was PCR amplified using 2 previously published primer pairs and a third unique primer pair designed for our new assay. Amplicons were sized using microfluidic chip analysis.Results: Concordance with the reference method was 100% (42/42). Intra-run and inter-run reproducibility were also 100% (3/3 and 3/3, respectively). The limit of detection was confirmed to be 6% mutant alleles.Conclusion: We determined that the microfluidic chip assay to detect CALR exon 9 mutations was acceptable for clinical use. Compared with the conventional method, the microfluidic analysis assay benefits from a streamlined workflow, faster turnaround, and a smaller instrument footprint.","PeriodicalId":94124,"journal":{"name":"Laboratory medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0