Journal of the American Association for Laboratory Animal Science : JAALAS最新文献

{"title":"Alfaxalone as a Total Intravenous Anesthesia Protocol in New Zealand White Rabbits (Oryctolagus cuniculus) Improves Cardiovascular Stability Compared to Isoflurane.","authors":"Julia Y Tsai, Erin E Palomera, Junko P Kundo, Katechan Jampachaisri, Cholawat Pacharinsak, Nicholas L Reyes","doi":"10.30802/AALAS-JAALAS-25-052","DOIUrl":"https://doi.org/10.30802/AALAS-JAALAS-25-052","url":null,"abstract":"<p><p>Alfaxalone has been studied for anesthetic induction of rabbits with rapid onset and a short duration of action; however, it has been minimally evaluated as an option for anesthetic maintenance. This study compared alfaxalone-based total intravenous anesthesia maintenance protocols against inhaled isoflurane, the current standard for anesthetic maintenance in rabbits. Twenty-four male New Zealand White rabbits were assigned to 1 of 3 treatment groups: isoflurane alone, alfaxalone with buprenorphine, or alfaxalone with midazolam. All rabbits were premedicated with buprenorphine HCl (0.02 mg/kg SC) and induced with alfaxalone (6 mg/kg IM). Following intubation and with supplementation of 100% O2, rabbits were maintained for 1 h on either isoflurane (2.5%) or alfaxalone continuous rate infusion (CRI) (0.2 mg/kg/min). For rabbits on the alfaxalone CRI, boluses of buprenorphine HCl (0.01 mg/kg IV or SC) or midazolam (0.1 to 0.3 mg/kg SC) were given upon induction or adjunctively as needed dependent on positive tail-pinch responses that were conducted at timepoints t0, t15, t30, t45, and t60. Heart rate, invasive blood pressure, respiratory rate, end-tidal CO2, percent O2 saturation, and temperature were recorded every 5 min. Surgical plane of anesthesia was characterized by lack of positive response to a tail clamp and was reached in all anesthetic groups. Results showed significant reduction in heart rate of the alfaxalone groups while there was no significant difference in mean arterial pressure compared with the isoflurane groups. However, respiratory rate in the alfaxalone groups was decreased with associated increases in end-tidal CO2 levels. There were no significant differences noted between alfaxalone treatment groups. The results confirmed that CRI alfaxalone (total intravenous anesthesia) should be considered as a potential anesthetic alternative to isoflurane anesthesia in rabbits, although special attention to respiratory monitoring and management is warranted.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144692881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"More Power for Less Money: Statistical, Power, and Cost Analyses That Account for Intracluster Correlation in Experiments with Same-Group Cage Mates.","authors":"Reid D Landes","doi":"10.30802/AALAS-JAALAS-24-094","DOIUrl":"10.30802/AALAS-JAALAS-24-094","url":null,"abstract":"<p><p>In experiments with cohoused animals, outcome variables can become correlated among cage mates. This is called intracluster correlation. When cage mates are all of the same group, the experiment is similar to a cluster randomized trial in human studies. Intracluster correlation in same-group cage mate experiments is a type of pseudoreplication, and ignoring it in statistical analyses increases false-positive results. Herein, we provide a tutorial on how to account for intracluster correlation in statistical analyses. Specifically, this is done by including cage identifiers as an independent variable in a linear mixed model, a type of ANOVA. Because power analyses must be based on the planned statistical analyses, we also include effect size calculations and sample size calculations (types of power analyses) in the tutorial. Effect size and sample size calculations help assure regulatory committees, such as IACUCs, granting agencies, and journals, that experiments are properly powered. These calculations will show that designing experiments to have more cages and fewer animals per cage is more efficient than fewer cages with more animals per cage. This statistical efficiency, which means more power, can be translated into reduced animal numbers, one of the 3Rs (replace, reduce, refine) of animal research. We then perform cost analyses and show how the costs of more cages with fewer animals overall are often less expensive than the costs of fewer cages with more animals overall. Altogether, accounting for intracluster correlation in the experiment design and analysis of same-group cage mate experiments results in fewer statistical errors, reduced costs, and fewer animals. Finally, analyses are demonstrated using JASP, a free, open-source, user-friendly statistical software, and provide R and SAS code to perform the analyses.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144661465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From the Archives, in Recognition of the 75th Anniversary of AALAS: The History of Commercial Production of Laboratory Rodents (1980).","authors":"Glen Otto","doi":"10.30802/AALAS-JAALAS-25-105","DOIUrl":"https://doi.org/10.30802/AALAS-JAALAS-25-105","url":null,"abstract":"","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144692884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Armenian Hamsters (Nothocricetulus migratorius): A New Host Susceptible to Corynebacterium bovis Infection and Disease.","authors":"Christopher Cheleuitte-Nieves, Ileana C Miranda, Abigail Michelson, Kelly Walton, Gail E Geist, Kourtney Nickerson, Juliette R Wipf, Sebastian E Carrasco, Sébastien Monette, Renata Mammone, Neil S Lipman","doi":"10.30802/AALAS-JAALAS-25-054","DOIUrl":"https://doi.org/10.30802/AALAS-JAALAS-25-054","url":null,"abstract":"<p><p>Corynebacterium bovis causes skin disease in immunocompromised mice and possibly rats. In 2022, scaly skin and mortality were observed in 7- to 11-d-old neonates (n = 8) from a primiparous Armenian (Nothocricetulus migratorius) hamster breeding pair in a newly established colony. C. bovis was detected by culture and PCR, and affected animals had moderate to severe acanthotic, hyperkeratotic lesions with intralesional C. bovis confirmed by in situ hybridization. Intrafollicular Demodex cricetuli mites, an ectoparasite found in all laboratory-maintained Armenian hamsters, were also identified in affected animals. To elucidate the role of D. cricetuli on C. bovis-associated disease and maintain adult hamsters without the need for sustained mite treatment, a D. cricetuli-free colony was generated by treating breeding pairs and their 1- to 3-d-old neonates with topical fluralaner (35 mg/kg), and a prospective study was undertaken to compare C. bovis-associated pup mortality in D. cricetuli-free and D. cricetuli-infested hamsters. During the ensuing 22 mo, 4 of 96 (4.2%) litters born exhibited C. bovis-associated disease and/or mortality. The litters were born to 4 different nulliparous breeding pairs (n = 47, 9%). Of the 4 affected litters, 2 were D. cricetuli-infested while 2 were D. cricetuli-free. C. bovis was routinely cultured with a variable bacterial burden that had no association with mortality or skin lesion severity from all hamsters, independent of their D. cricetuli status. The severity of histologic pathology appeared to correlate with clinical presentation and mortality in neonates. Whole genome sequencing was performed on 4 hamster C. bovis isolates, which revealed a close genetic association among the isolates as well as with previously characterized mouse and rat C. bovis isolates.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144692882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved Detection of Murine Pathogens Using Sentinel-Free Media Compared to Live Animal Sampling.","authors":"Joshua M Woolsey, Jennie Bonica, Savannah Godbey, Ida M Washington","doi":"10.30802/AALAS-JAALAS-25-070","DOIUrl":"https://doi.org/10.30802/AALAS-JAALAS-25-070","url":null,"abstract":"<p><p>Health monitoring of rodent colonies has traditionally used live animal (LA) sampling by means such as the use of soiled bedding sentinels (SBS), with the associated expenditure of labor, supplies, and animals. In the spirit of the 3Rs, sentinel-free (SF) approaches are becoming more common. PCR testing of environmental samples is replacing traditional SBS-based testing for routine health monitoring of rodent colonies. Passive sampling of in-cage media exposed to pooled, soiled bedding is effective for detecting some common rodent pathogens. We hypothesized that PCR testing of commercially available media exposed to soiled bedding would be as effective as sampling SBS, or SBS combined with samples from colony animals, for detecting several enzootic organisms of mice (Mus musculus) within our facility. Media were placed in IVC cages and exposed to pooled dirty bedding from all cages on a rack side at biweekly cage changes during a 3-mo period. PCR results of the SF soiled bedding-exposed media were compared with results from feces, pelt, and oral swabs from SBS with and without SBS combined with 10 randomly sampled colony animals from the same rack side over the same period. Detection rates were similar for murine norovirus and Staphylococcus xylosus using SF testing compared with SBS with and without direct colony samples. Five organisms, Proteus mirabilis, Rodentibacter heylii, Staphylococcus aureus, Klebsiella oxytoca, and Klebsiella pneumoniae, were detected by SF testing, but not by LA samples. Demodex musculi, Entamoeba, Proteus mirabilis, Helicobacter spp., and Rodentibacter heylii were detected at significantly higher rates by SF testing compared with SBS with and without colony animal samples. SF testing detected organisms of zoonotic concern (S. aureus, K. pneumoniae) that were undetected by LA testing. SF testing detected organisms at similar rates during 2 consecutive quarters. We conclude that PCR testing of media exposed to pooled soiled bedding effectively detects these common enzootic organisms.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144692885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel 3D-Printed Mouse Model for Surgical Training: Multicenter Construct, Face, and Content Validation Study.","authors":"Diego Celdran-Bonafonte, Felix Gantenbein, Daniel Ruiz Perez, Regina Rumpel, Nina Eva Trimmel","doi":"10.30802/AALAS-JAALAS-25-064","DOIUrl":"https://doi.org/10.30802/AALAS-JAALAS-25-064","url":null,"abstract":"<p><p>Advancements in laboratory animal training increasingly incorporate technological innovations aiming to better align training standards with the 3Rs (Replacement, Reduction, and Refinement). This trend is shifting away from traditional reliance on live animals and cadavers toward simulation-based methods. This study introduces and assesses the validity of a novel 3D-printed rodent surgical simulator designed for the practice and training of basic rodent surgical skills. To evaluate its potential to partially replace animal use, refine rodent surgical training, and reduce the number of animals needed, a multicenter validation study across 5 European and US research academic centers was conducted. The study assessed the simulator's face, content, and construct validity, involving participants inexperienced and experts in rodent surgery. The construct validity was evaluated through task completion times and blinded quality assessments across multiple training iterations. The results revealed that inexperienced participants demonstrated significant improvements in both speed and quality of surgical tasks with repeated simulator use, eventually reaching performance levels comparable to experts' initial attempts. Expert participants consistently outperformed the inexperienced group. Face and content validity were supported by postuse surveys, with high ratings from both groups regarding the simulator's anatomic realism and its perceived usefulness for the acquisition and development of fundamental surgical skills. Overall, the findings of this study support that this 3D-printed rodent surgical simulator offers a realistic, effective, and ethically sound alternative for basic rodent surgical skills training and competency assessment.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144669185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of 415-nm Blue Light and Hypochlorous Acid on the Rhesus Macaque (Macaca mulatta) Cranial Implant Microbiota.","authors":"Mia T Lieberman","doi":"10.30802/AALAS-JAALAS-25-042","DOIUrl":"10.30802/AALAS-JAALAS-25-042","url":null,"abstract":"<p><p>Cranially-implanted macaques are a crucial model for neuroscience research. Implant complications include abscesses, meningoencephalitis, and implant-tissue margin infections. Antimicrobial overuse has increased bacterial resistance, risking macaque health and complicating treatment of infections. This project aimed to assess the antimicrobial effects of blue light (∼415 nm) and hypochlorous acid (HOCl) treatment of cranial implant margins. Blue light exerts antibacterial effects via the induction of reactive oxygen species. We hypothesized that exposing cranial implant margins to a commercially available blue light device followed by HOCl treatment would improve clinical appearance and decrease bacterial burden as assessed by aerobic/anaerobic culture and tissue margin microbiota analysis (decreased α and β diversity and altered taxonomic composition). Eight rhesus macaques were exposed to 6 min of blue light followed by 0.024% HOCl solution three times weekly for 4 wk. Swabs for microbiota analysis and bacterial cultures were collected before and 24 h after the last treatment session. Control microbiota swabs were collected from a separate implant margin area only exposed to HOCl. All animals tolerated the blue light exposure but had varied improvement in margin clinical appearance. The most common bacteria identified on culture were Staphylococcus aureus (n = 8), β-hemolytic streptococcus (n = 8), and Corynebacterium ulcerans (n = 6). Microbiota analysis of the 16S rRNA V4 gene region demonstrated many anaerobic operational taxonomic units in addition to the aerobic species cultured, highlighting limitations of culture-based methods. All animals had unique microbiota taxonomic profiles with a mean of 84 operational taxonomic units and a median Shannon diversity index of ∼2.6. No significant differences were found between treatment groups, α diversity, or β diversity before and after the study. The effectiveness of blue light therapy likely relates to the device power and depth of penetration into the tissue margin. While safe, future work is needed to optimize the dose and delivery methods of light-based therapies.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12379613/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144669132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Pharmacokinetics of Tulathromycin Following Subcutaneous Administration in Rhesus Macaques (Macaca mulatta).","authors":"Jacob D Herford, Luke A Wittenburg, Rebecca L Sammak, Kelsey E Carroll, Diane E Stockinger","doi":"10.30802/AALAS-JAALAS-25-025","DOIUrl":"https://doi.org/10.30802/AALAS-JAALAS-25-025","url":null,"abstract":"<p><p>Campylobacter jejuni is a pathogenic bacterium commonly associated with enteritis and diarrhea in rhesus macaques (Macaca mulatta). The standard therapy at the California National Primate Research Center is oral azithromycin, a second-generation macrolide, given daily for 5 d. Oral treatment administration can be difficult with some animals. Poor oral compliance for antibiotics can result in treatment failure and potentially select for antibiotic resistance. Tulathromycin, a newer-generation macrolide, may offer an injectable alternative to azithromycin. The aim of the current study is to quantify the pharmacokinetics of tulathromycin in plasma in rhesus macaques. Six rhesus macaques were each given a single 2.5 mg/kg dose SC of tulathromycin, and serial blood samples were collected at 0, 0.25, 0.5, 0.75, 1.0, 1.5, 2, 4, 8, 12, 24, 48, 72, 96, 120, and 168 h, to quantify the concentration of tulathromycin in plasma over time. Results show that Cmax = 1,280 ± 395 ng/mL, Tmax = 1.25 ± 0.5 h, t1/2 = 77.2 ± 15.4 h, and AUC0-168 = 6,557.4 ± 875.4 h·ng/mL. There are no published Clinical and Laboratory Sciences Institute breakpoints for tulathromycin against C. jejuni, but based on an independently established minimum inhibitory concentration of 500 ng/mL, these data suggest that 2.5 mg/kg tulathromycin can be given subcutaneously to achieve potential therapeutic levels in rhesus macaques, possibly providing an alternative to oral azithromycin.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144762882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Best Practices for Supporting Professional Identity Formation during Laboratory Animal Medicine Residency Training.","authors":"Megan H Nowland, Paul Haidet, Heather Stuckey, Tiffany L Whitcomb","doi":"10.30802/AALAS-JAALAS-25-047","DOIUrl":"https://doi.org/10.30802/AALAS-JAALAS-25-047","url":null,"abstract":"<p><p>Professional identity formation (PIF) is the dynamic psychosocial process crucial to becoming enculturated into a profession. This nonlinear process starts with entrance into a training program and ideally results in the harmonization of norms, values, and aspirations of the person with those of the chosen field. PIF is most successful when trainees can reflect, have positive role models and mentors, and receive social validation. Those who experience lack of support or hardship during PIF are at risk of burnout and poor mental health during their careers. Medical education research indicates that residency training significantly impacts PIF. This occurs because residents' new patient care responsibilities force them to grapple with the differences between the ideal practice taught in medical school and the realities of their work lives. Emerging evidence in veterinary medical education research indicates that residents enrolled in laboratory animal medicine (LAM) residency programs experience similar tensions as they navigate relationships with stakeholders, encounter conditional valuation of their veterinary knowledge, and feel powerless to enact changes. To explore best practices for supporting PIF for developing LAM veterinarians, we performed a qualitative analysis of written comments from surveys completed by attendees at the 2022 American College of Laboratory Animal Medicine Forum meeting. Results were organized by 5 themes: (1) empowering residents to step into their authority and understand the limitations of their roles, (2) providing support for learning about the complexities of relationships within LAM, (3) serving as role models and mentors in building relationships, (4) creating opportunities to learn professional communication, and (5) advocating for the specialized and explicit value of the profession (LAM). From these results, we constructed a model that illustrates the PIF process for LAM veterinarians in training, with the goal of raising awareness of the connection between the quality of PIF and career wellness.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144669190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baseline Characterization of Swine Leukocyte Antigen Diversity in GFP-Expressing Domestic Swine.","authors":"Anshaal Furrukh, Herra Javed, Kristin Whitworth, Louis Steen, Chak-Sum Ho, Malik F Ayyad, Taufiek Konrad Rajab","doi":"10.30802/AALAS-JAALAS-25-022","DOIUrl":"10.30802/AALAS-JAALAS-25-022","url":null,"abstract":"<p><p>Yorkshire piglets serve as valuable animal models in biomedical research, including partial heart transplantation research. This study characterizes the swine leukocyte antigen (SLA) diversity within a cohort of 16 Yorkshire piglets, including 5 genetically modified to express green fluorescent protein (GFP). Employing SLA typing, we identified 25 unique SLA class I alleles (9 SLA-1, 11 SLA-2, 5 SLA-3) and 17 unique SLA class II alleles (8 DRB1, 6 DQB1, 3 DQA). Notably, an allele of SLA-1*08:XX was detected in 75% of the piglets, while haplotypes Lr-7.26 and Lr-6.12b were most frequent, occurring at 18.75%. Comparative analysis with previous studies revealed consistent genetic trends, although differences in haplotype frequencies underscore the influence of breeding practices and sample size on SLA allele and haplotype distribution. Our findings highlight the significant polymorphism in the SLA complex of Yorkshire piglets, which is relevant for their utility as preclinical models for studying transplantation outcomes. The characterization of GFP piglets provides crucial genomic data for future research in cell tracking and graft integration. While the study's relatively small cohort may limit its generalizability, it represents the first baseline SLA typing of GFP Yorkshire piglets, offering foundational insights into their genetic diversity. This work emphasizes the importance of standardized genetic mapping to enhance the reproducibility and utility of laboratory swine in biomedical research.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12379703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144669188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}