Human & experimental toxicology最新文献_第4页

Protective effects of dexmedetomidine against propofol-induced memory impairment in developing rat involved Src and RARα. 右美托咪定对异丙酚诱导的大鼠Src和RARα记忆损伤的保护作用。

Human & experimental toxicology Pub Date : 2025-01-01 Epub Date: 2025-04-29 DOI: 10.1177/09603271251336467

Xiaoyan Xu, Jianmei Yang, Qiang Jia, Qianqian Hu, Huiling Liu

{"title":"Protective effects of dexmedetomidine against propofol-induced memory impairment in developing rat involved Src and RARα.","authors":"Xiaoyan Xu, Jianmei Yang, Qiang Jia, Qianqian Hu, Huiling Liu","doi":"10.1177/09603271251336467","DOIUrl":"https://doi.org/10.1177/09603271251336467","url":null,"abstract":"BackgroundDexmedetomidine (DEX) can offer protection to the nervous, urinary and circulatory systems. It can alleviate local oxidative stress, reduce inflammatory responses, inhibite cellular autophagy and decrease apoptosis.AimTo explore the potential protection and possible mechanisms of DEX against propofol (PPF)-induced memory impairment in developing rats.Material and methodsThe effects of DEX on spatial learning and passive avoidance abilities of rats were evaluated using eight-arm mirror maze and passive avoidance experiments. mRNA levels were detected using RT-qPCR analysis while protein levels were determined using western blot. A network pharmacology approach was used to predict potential targets of DEX against PPF-induced memory impairment. The cell autophagy and apoptosis were detected using commercial kits.ResultsDEX improved the impairment of developing rats on spatial learning and passive avoidance caused by PPF exposure. DEX regulates autophagic activity to inhibit neuronal apoptosis. RARα and Src were potential targets for DEX against memory impairment caused by PPF exposure. DEX upregulated the expression levels of Bdnf, p-CREB/CREB, p-Akt/Akt, and p-TrkB/TrkB proteins.ConclusionDEX may regulate Bdnf/TrkB and activate the activity of the PI3K/Akt signaling pathway by targeting RARα and Src, thereby inhibiting excessive autophagy and alleviating memory impairment.","PeriodicalId":94029,"journal":{"name":"Human & experimental toxicology","volume":"44 ","pages":"9603271251336467"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144045608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of grape seed extract on doxorubicin-induced testicular and epididymal damage in rats. 葡萄籽提取物对多柔比星诱发的大鼠睾丸和附睾损伤的影响

Human & experimental toxicology Pub Date : 2025-01-01 Epub Date: 2025-03-14 DOI: 10.1177/09603271251319787

Emine Sarman, Halit Bugra Koca

{"title":"Effect of grape seed extract on doxorubicin-induced testicular and epididymal damage in rats.","authors":"Emine Sarman, Halit Bugra Koca","doi":"10.1177/09603271251319787","DOIUrl":"10.1177/09603271251319787","url":null,"abstract":"IntroductionDoxorubicin (DXR), a chemotherapeutic antibiotic, is widely used as an anticancer drug in clinics. Grape seed extract is known for its potent antioxidant properties. The aim of this study is to investigate the effect of high-antioxidant content Vitis vinifera L. seed extract against DXR-induced testicular and epididymal damage.Methods30 male rats were randomly divided into five groups with six animals in each group: Control, Sham, DXR (a single i.p. dose of 15 mg/kg), DXR + VIT (120 mg/kg VIT seed extract via gavage for 14 days and a single i.p. dose of DXR (15 mg/kg) on day 5, VIT (120 mg/kg VIT seed extract via gavage for 14 days). Animals were sacrificed under anesthesia 24 hours after the last drug administration, and blood, testis, and epididymis tissues were collected.ResultsTissues from the DXR group exhibited atrophic seminiferous tubules, Leydig cell degeneration, tunica albuginea and basal membrane thinning, immature spermatogenic cells, vascular congestion, epididymal atrophy, epithelial cell deletion, decreased sperm count, increased connective tissue, and absence of sperm in the lumen. Serum levels of interleukin 6 (IL-6), interleukin 1β (IL-1β), Tumor Necrosis Factor α (TNF-α), Total Oxidant Status (TOS), Total Antioxidant Status (TAS), and testosterone were increased in the DXR group, while interleukin 10 (IL-10) levels were decreased. The DXR + VIT group showed a near-recovery similar to the control.ConclusionDXR increased oxidative stress, apoptosis, and inflammation in the testis and epididymis, whereas VIT exhibited protective effects against these damages.","PeriodicalId":94029,"journal":{"name":"Human & experimental toxicology","volume":"44 ","pages":"9603271251319787"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nomogram for predicting mechanical ventilation need among acutely intoxicated patients with impaired consciousness: Correspondence. 预测意识障碍的急性中毒患者机械通气需求的提名图：通讯。

Human & experimental toxicology Pub Date : 2024-01-01 DOI: 10.1177/09603271241285992

Hineptch Daungsupawong, Viroj Wiwanitkit

引用次数: 0

Performance assessment of new Poisoning Mortality Score and PGI score for predicting mortality in patients with acute aluminum phosphide poisoning. 预测急性磷化铝中毒患者死亡率的新中毒死亡率评分和 PGI 评分的性能评估。

Human & experimental toxicology Pub Date : 2024-01-01 DOI: 10.1177/09603271241302208

Ghada N El-Sarnagawy, Amira A Abdelnoor, Mona M Ghonem

{"title":"Performance assessment of new Poisoning Mortality Score and PGI score for predicting mortality in patients with acute aluminum phosphide poisoning.","authors":"Ghada N El-Sarnagawy, Amira A Abdelnoor, Mona M Ghonem","doi":"10.1177/09603271241302208","DOIUrl":"https://doi.org/10.1177/09603271241302208","url":null,"abstract":"Background: Until now, no definite standardized method has been used to promptly assess the severity and outcome of acute aluminum phosphide (ALP) poisoning. The current study aimed to evaluate the performance of the new Poisoning Mortality Score (PMS) and PGI score for predicting mortality in acute ALP-poisoned patients, highlighting the accuracy of new PMS components.Patients and methods: A 2-year cross-sectional study was conducted on ALP-poisoned patients admitted to Tanta University Poison Control Centre from April 2021 to March 2023. Socio-demographics, poisoning data, and initial vital signs were recorded. Additionally, new PMS and PGI scores were calculated on admission. Patients were categorized according to the mortality outcome into survivors and nonsurvivors.Results: Out of 160 included ALP poisoned patients, mortality was recorded in 112 (70%) patients. The nonsurvivors had significantly higher median PGI and new PMS values than survivors. New PMS, vital signs component of new PMS, and PGI conveyed good discriminatory power for predicting mortality (AUC = 0.883, 0.873, and 0.817, respectively). Although the new PMS outperformed PGI in all predictive metrics, no significant difference in AUCs was observed between the new PMS and its vital signs component.Conclusion: The new PMS vital signs component is closely aligned with the new PMS. Thus, it can be used as a valid, comprehensive, and practical tool to substitute the whole score calculation for rapid ALP-poisoned patient assessment to enhance emergency clinical decision-making.","PeriodicalId":94029,"journal":{"name":"Human & experimental toxicology","volume":"43 ","pages":"9603271241302208"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outbreaks of mushroom poisoning associated with acute kidney injury. 与急性肾损伤相关的蘑菇中毒爆发。

Human & experimental toxicology Pub Date : 2024-01-01 DOI: 10.1177/09603271241304368

Zelal Adibelli, Hayrunisa Bas Sermenli, Ziynet Alphan Uc

{"title":"Outbreaks of mushroom poisoning associated with acute kidney injury.","authors":"Zelal Adibelli, Hayrunisa Bas Sermenli, Ziynet Alphan Uc","doi":"10.1177/09603271241304368","DOIUrl":"https://doi.org/10.1177/09603271241304368","url":null,"abstract":"Introduction: The outbreak of acute kidney injury (AKI) due to mushroom poisoning is not a frequently encountered medical challenge. Herein, we present 13 mushroom poisoning cases associated with AKI related to Amanita Proxima (A. Proxima) causing poisoning reported in a short time period in Turkey.Methods: A total of 13 patients with AKI due to mushroom poisoning admitted to Usak Research and Training Hospital between November and December 2020 were included. Under morphological and microscopical investigations of mushroom specimens (from three patients), the species of the mushrooms were identified.Results: The median age of 13 patients presenting with AKI due to mushroom poisoning was 55 (ranging between 19 and 72 years), and 60.4% were males. Nausea and vomiting were the first symptoms in most patients and appeared at a mean time of 12.8 ± 7.6 h after ingesting mushrooms. Mean serum creatinine on admission was 7.2 ± 3.8 mg/dL. Kidney replacement therapy (KRT) was administered to all patients, and mortality occurred in two due to sepsis and heart failure (HF). Species of the mushroom specimens obtained from three patients were identified as A. Proxima, a rarely encountered type of mushroom. A. Proxima has a considerable similarity to a common and edible species specific to the Mediterranean Basin, known as A. Ovoidea.Discussion: Based on our findings, we emphasize the consideration of nephrotoxic mushrooms of the genus Amanita in the evaluation of mushroom poisoning cases, as well as the efforts needed to increase public awareness regarding the risk of fatal outcomes of consuming wild mushrooms.","PeriodicalId":94029,"journal":{"name":"Human & experimental toxicology","volume":"43 ","pages":"9603271241304368"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142718084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Persistent diazinon induced neurotoxicity: The effect on inhibitory avoidance memory performance, amyloid precursor proteins, and TNF-α levels in the prefrontal cortex of rats. 持续性二嗪农诱导的神经毒性：对大鼠前额叶皮层抑制性回避记忆能力、淀粉样前体蛋白和 TNF-α 水平的影响

Human & experimental toxicology Pub Date : 2024-01-01 DOI: 10.1177/09603271241235408

Salva Afshari, Mehdi Sarailoo, Vahid Asghariazar, Elham Safarzadeh, Masoomeh Dadkhah

{"title":"Persistent diazinon induced neurotoxicity: The effect on inhibitory avoidance memory performance, amyloid precursor proteins, and TNF-α levels in the prefrontal cortex of rats.","authors":"Salva Afshari, Mehdi Sarailoo, Vahid Asghariazar, Elham Safarzadeh, Masoomeh Dadkhah","doi":"10.1177/09603271241235408","DOIUrl":"10.1177/09603271241235408","url":null,"abstract":"Introduction: Organophosphate pesticides (Ops) like diazinon (DZN) have well-known neurotoxic effects and low-level chronic exposure has been linked to detrimental neurobehavioral impairments and memory deficits. However, it's not entirely clear how DZN-induced biological changes, particularly in the prefrontal cortex (PFC) contribute to these effects. The purpose of this study is to investigate the impact of DZN exposure on inhibitory avoidance (IA) memory function, amyloid precursor expression (APP), and proinflammatory tumor necrosis factor-α (TNF-α) levels in the rat cortex.Materials and methods: Rats were divided into 4 groups and recived 2 mg/kg DZN for 5-days or 12-weeks and two control groups recived the same volume of vehicle. IA memory was assesed using the shuttle box apparatus. Rats were sacrificed and the prefrontal cortex PFC were removed. Real-time PCR and Western blotting were used to messure TNF-α, and amyloid protein precursors gene expression and protein levels.Results: Our findings indicated that DZN caused body weight loss and a notable decline in performance on the IA memory. Additionally, 5-days exposure increased APP and APLP2 protein levels in the PFC, while 12-weeks exposure decreased these levels. Furthermore, expression of APP and APLP2 gens were decreased in PFC. TNF-α levels increased as a result of 5-days exposure to DZN, but these levels dropped to normal after 12-weeks administration, and this observation was significant.Conclusion: Taken together, exposure to low doses of DZN leads to disturbances in IA memory performance and also alternations in amyloid beta precursors that can be related to increased risk of Alzheimer's disease.","PeriodicalId":94029,"journal":{"name":"Human & experimental toxicology","volume":"43 ","pages":"9603271241235408"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140112517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling thioacetamide-induced toxicity: Multi-organ damage and omitted bone toxicity. 揭示硫代乙酰胺诱导的毒性：多器官损伤和遗漏的骨毒性。

Human & experimental toxicology Pub Date : 2024-01-01 DOI: 10.1177/09603271241241807

Haodong Zhang, Jian Xu

{"title":"Unveiling thioacetamide-induced toxicity: Multi-organ damage and omitted bone toxicity.","authors":"Haodong Zhang, Jian Xu","doi":"10.1177/09603271241241807","DOIUrl":"10.1177/09603271241241807","url":null,"abstract":"Thioacetamide (TAA), a widely employed hepatotoxic substance, has gained significant traction in the induction of liver failure disease models. Upon administration of TAA to experimental animals, the production of potent oxidative derivatives ensues, culminating in the activation of oxidative stress and subsequent infliction of severe damage upon multiple organs via dissemination through the bloodstream. This review summarized the various organ damages and corresponding mechanistic explanations observed in previous studies using TAA in toxicological animal experiments. The principal pathological consequences arising from TAA exposure encompass oxidative stress, inflammation, lipid peroxidation, fibrosis, apoptosis induction, DNA damage, and osteoclast formation. Recent in vivo and in vitro studies on TAA bone toxicity have confirmed that long-term high-dose use of TAA not only induces liver damage in experimental animals but also accompanies bone damage, which was neglected for a long time. By using TAA to model diseases in experimental animals and controlling TAA dosage, duration of use, and animal exposure environment, we can induce various organ injury models. It should be noted that TAA-induced injuries have a time-dependent effect. Finally, in our daily lives, especially for researchers, we should take precautions to minimize TAA exposure and reduce the probability of related organ injuries.","PeriodicalId":94029,"journal":{"name":"Human & experimental toxicology","volume":"43 ","pages":"9603271241241807"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140295674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and validation of a nomogram for predicting mechanical ventilation need among acutely intoxicated patients with impaired consciousness. 开发并验证用于预测意识受损的急性中毒患者机械通气需求的提名图。

Human & experimental toxicology Pub Date : 2024-01-01 DOI: 10.1177/09603271241267214

Heba Ibrahim Lashin, Fatma Gaber Sobeeh, Zahraa Khalifa Sobh

{"title":"Development and validation of a nomogram for predicting mechanical ventilation need among acutely intoxicated patients with impaired consciousness.","authors":"Heba Ibrahim Lashin, Fatma Gaber Sobeeh, Zahraa Khalifa Sobh","doi":"10.1177/09603271241267214","DOIUrl":"https://doi.org/10.1177/09603271241267214","url":null,"abstract":"Background: A considerable portion of acutely intoxicated patients is presented with impaired consciousness. Early identification of those patients who require advanced medical care, such as mechanical ventilation (MV), can improve their prognosis.Methods: This study included 330 acutely intoxicated patients who were presented with impaired consciousness and admitted to Tanta University Poison Control Center, Egypt, in the period from January 2021 to December 2023. Patients were enrolled in derivation (257 patients) and validation (73 patients) cohorts. Patients' data were analyzed to develop and validate a predictive nomogram to determine the probability of MV need in acutely intoxicated patients.Results: Significant predictors for MV need were mean arterial blood pressure (OR = 0.96, p = .014), PaO2 (OR = 0.96, p = .001), pH (OR = 0.00, p < . 001), and glucose/potassium ratio (OR = 1.59, p = .030). These four parameters were used to formulate a bedside nomogram. Receiver-operating characteristic (ROC) analysis for the proposed nomogram shows that area under the curve (AUC) = 95.7%, accuracy = 93.4%, sensitivity = 88.9%, and specificity = 95.1%. The internal validation for the developed nomogram was assessed using a bootstrapping method and calibration curve. Regarding external validation, AUCs for the developed nomogram probability was 96.5%, and for predicted probability using the developed nomogram was 97.8%.Conclusion: The current study provides a validated nomogram that could be used as a reliable tool for the accurate prediction of MV need among acutely intoxicated patients with impaired consciousness. It could assist in the early identification of patients who will require MV, especially in low-income countries with limited resources.","PeriodicalId":94029,"journal":{"name":"Human & experimental toxicology","volume":"43 ","pages":"9603271241267214"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141879977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential induction of hyperkeratosis in rats' cervi by gentamicin via induction of oxidative stress, inflammation and apoptosis. 庆大霉素通过诱导氧化应激、炎症和细胞凋亡可能诱导大鼠宫颈角化过度。

Human & experimental toxicology Pub Date : 2024-01-01 DOI: 10.1177/09603271231225744

Walaa Yehia Abdelzaher, Mina Ezzat Attya, Mohamed Ahmed Zeen El-Din, Alaa Gamal El Satae, Hatem A Mohamed, Remon R Rofaeil

引用次数: 0

Retrospective analysis of the etiology and drugs for vitreous hemorrhage caused by non-diabetic retinopathy and non-traumatic factors. 对非糖尿病视网膜病变和非创伤性因素导致的玻璃体出血的病因和药物进行回顾性分析。

Human & experimental toxicology Pub Date : 2024-01-01 DOI: 10.1177/09603271241303411

MingXia Dong, Qing Xiao, YunTeng Mei, Bao Liang Fan, ChenWei Liu

{"title":"Retrospective analysis of the etiology and drugs for vitreous hemorrhage caused by non-diabetic retinopathy and non-traumatic factors.","authors":"MingXia Dong, Qing Xiao, YunTeng Mei, Bao Liang Fan, ChenWei Liu","doi":"10.1177/09603271241303411","DOIUrl":"https://doi.org/10.1177/09603271241303411","url":null,"abstract":"Objective: To retrospectively analyze the etiology of non-diabetic retinopathy (DR) and non-traumatic vitreous haemorrhage (VH), and the effects of different anti-vascular endothelial growth factor (VEGF) drugs.Methods: A retrospective analysis was conducted on VH patients diagnosed as non-diabetic retinopathy or trauma. Among 101 patients treated with anti-VEGF drugs, there were 48 cases in the Conbercept group and 53 cases in the Ranibizumab group. The causes of bleeding and gender distribution of the included cases were analyzed.Results: In cases of retinal vein occlusion, the proportion of males was much higher than females (p < 0.05). After treatment, the best corrected visual acuity (BCVA), intraocular pressure, central macular thickness (CMT), aqueous humor VEGF, TNF-α, IL-10, and IL-6 of the two groups showed a decreasing trend (p < 0.05). The Conbercept group had markedly lower CMT than the Ranibizumab group (p < 0.05). In addition, there existed no significant statistical differences between the two groups in terms of BCVA, intraocular pressure, aqueous humor VEGF, TNF-α, IL-10, IL-6, incidence of adverse reactions, and recurrence rate (p > 0.05).Conclusion: In patients with non-DR and traumatic VH, retinal vein occlusion, perivenous retinitis, retinal tears/detachment, exudative AMD, and polypoidal choroidal vasculopathy were the main etiologies. Conbercept and Ranibizumab had comparable efficacy and could effectively improve visual acuity and aqueous humor inflammation, with high safety and low recurrence rate. Conbercept had a more pronounced effect on the reduction of CMT in patients.","PeriodicalId":94029,"journal":{"name":"Human & experimental toxicology","volume":"43 ","pages":"9603271241303411"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142735417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0