Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology最新文献

{"title":"[Long-term prognosis and influencing factors of anterior composite resin restoration].","authors":"Weiyang Chen, Feiyang Wu, Xi Wei","doi":"10.7518/hxkq.2025.2025305","DOIUrl":"10.7518/hxkq.2025.2025305","url":null,"abstract":"<p><p>With the increasing demand for dental aesthetic outcomes, techniques for composite resin restoration intended for anterior teeth have been widely applied due to their minimally invasive and superior esthetic performance. Despite promising short-term outcomes, the long-term prognosis of anterior resin restorations remains challenging. Frequently reported complications include restoration fractures and decoloration. Material selection, operative procedures, and patient-related factors can affect the long-term outcomes of restorations. This review aims to systematically analyze the long-term clinical performance of resin restorations in anterior teeth. The key factors influencing treatment efficacy are also investigated. The findings are expected to provide a basis for optimizing clinical strategies in procedures for anterior composite resin restoration.</p>","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"43 6","pages":"797-807"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12680119/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145662846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Correlation analysis of cell-free DNA in gingival crevicular fluid with periodontal clinical indicators and cyclic guanosine phosphate-adenosine phosphate synthase-stimulator of interferon genes signaling pathway].","authors":"Lan Chen, Xuanzhi Zhu, Jieyu Zhou, Jiyao Li, Lei Zhao","doi":"10.7518/hxkq.2025.2024391","DOIUrl":"10.7518/hxkq.2025.2024391","url":null,"abstract":"<p><strong>Objectives: </strong>This study aims to explore the potential relationships of cell-free DNA (cfDNA) in gingival crevicular fluid (GCF) with periodontal clinical indicators and the expression of DNA receptor pathway cyclic guanosine phosphate-adenosine phosphate synthase (cGAS)-stimulator of interferon genes (STING) in gingival tissues and human gingival fibroblasts (HGFs).</p><p><strong>Methods: </strong>GCF and gingival tissue samples were collected from periodontally healthy individuals and patients diagnosed with periodontitis. Periodontal clinical indicators were recorded, including plaque index (PLT), bleeding index (BI), probing depth (PD), and clinical attachment level (CAL). The concentration of cfDNA in GCF was quantified, and the correlation between GCF and periodontal clinical indicators was analyzed. Immunofluorescence and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) were used to assess the distribution of cGAS, STING, and p-STING in gingival tissues. Additionally, the mRNA expression levels of the key components of the cGAS-STING signaling pathway, namely, cGAS, STING, inhibitory of kappa-B kinase (IKK), nuclear factor kappa-B p65 (NF-κB p65), interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α), were measured. Furthermore, cfDNA extracted from GCF was employed to stimulate HGFs in the healthy control and periodontitis groups, and the mRNA expression levels of the key molecules of cGAS-STING signaling pathway were detected through Western blot and RT-qPCR.</p><p><strong>Results: </strong>The concentration of cfDNA in GCF was found to be significantly elevated in the periodontitis group compared with the control group. Moreover, cfDNA concentration demonstrated a strong positive correlation with the periodontal clinical indicators. Immunofluorescence analysis revealed considerably increased percentage of fluorescence co-localization of cGAS, STING, and p-STING with the gingival fibroblast FSP-1 marker in the gingival tissues of the periodontitis group. The mRNA expression levels of cGAS, STING, IKK, NF-κB p65, IL-1β, IL-6,and TNF-α were significantly higher in the periodontitis group. <i>In vitro</i> stimulation of HGFs with GCF-derived cfDNA resulted in increased protein expression of cGAS and p-STING and considerably upregulated the mRNA expression levels of cGAS, STING, IKK, NF-κB p65, IL-1β, IL-6, and TNF-α in the healthy and periodontitis groups compared with the blank group. Correlation analysis showed that the concentration of cfDNA at the sampling site was positively correlated with the mRNA expression levels of cGAS, STING, NF-κB p65, and IL-6 in gingival tissues.</p><p><strong>Conclusions: </strong>cfDNA concentrations in the GCF of patients with periodontitis are considerably elevated, and are associated with the activation of the cGAS-STING signaling pathway in HGFs. These findings suggest that cfDNA contributes to the progression of periodontitis.</p>","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"43 6","pages":"808-818"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12680112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145662885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Mediation analysis of plasma phosphorylated tau217 in the association between periodontitis and mild cognitive impairment].","authors":"Yanbiao Zhang, Meirong Wei, Xuejuan Zhao, Xiaolei Qi, Shanshan Zuo, Shumei Mao, Jun Wang, Gang Ding","doi":"10.7518/hxkq.2025.2025078","DOIUrl":"10.7518/hxkq.2025.2025078","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;This study aimed to investigate the potential mediating role of plasma phosphorylated tau217 (p-tau217) in the association between periodontitis and mild cognitive impairment (MCI).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;In this case-control study, patients diagnosed with MCI in the Neurology Department of the First Affiliated Hospital of Shandong Second Medical University from November 2023 to May 2024 were selected as the case group (MCI group). Cognitively normal (CN) volunteers, matched for age and education level and recruited from the physical examination center during the same period, served as the control group (CN group). The general demographic data of the study participants were collected. The Beijing versions of the Montreal Cognitive Assessment (MoCA), clinical dementia rating (CDR), and activities of daily living scale (ADL) were used to assess neuropsychological functions. Clinical periodontal examinations were conducted, the periodontal inflamed surface area (PISA) was calculated, and the periodontitis stage was determined in accordance with the 2018 classification. Fasting elbow venous blood samples were collected in the morning, and blood biochemical indicators were measured. Plasma p-tau217 levels were detected using enzyme-linked immunosorbent assay (ELISA). Statistical analyses were performed using &lt;i&gt;t&lt;/i&gt;-test, Mann-Whitney &lt;i&gt;U&lt;/i&gt; test, chi-square test, partial correlation analysis, multivariate Logistic regression analysis, multiple linear regression analysis, restricted cubic spline (RCS) regression analysis, and mediation effect analysis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Among the 192 participants, 96 belong to the MCI group and 96 to the CN group. The prevalence of periodontitis was 63.5% in the MCI group and 43.8% in the CN group, with a statistically significant difference (χ²=7.561, &lt;i&gt;P&lt;/i&gt;=0.006). The plasma p-tau217 levels in the MCI group were significantly higher than those in the CN group [7.00 (4.27-9.65) ng/mL versus 2.02 (0.80-3.81) ng/mL, &lt;i&gt;Z&lt;/i&gt;=-8.108, &lt;i&gt;P&lt;/i&gt;&lt;0.001]. Partial correlation analysis revealed that plasma p-tau217 levels were positively correlated with all the clinical periodontal indices (all &lt;i&gt;P&lt;/i&gt;&lt;0.001). After adjustments for baseline covariates, multivariate Logistic regression indicated that periodontitis was an independent risk factor for MCI. Patients with periodontitis had a 1.977-fold higher MCI risk than those without periodontitis (OR=1.977, 95%CI: 1.088-3.594, &lt;i&gt;P&lt;/i&gt;=0.025). Moreover, the MCI risk for stage Ⅰ/Ⅱ periodontitis and stage Ⅲ/Ⅳ periodontitis was 1.878 times (OR=1.878, 95%CI: 1.029-3.425, &lt;i&gt;P&lt;/i&gt;=0.040) and 2.625 times (OR=2.625, 95%CI: 1.073-6.246, &lt;i&gt;P&lt;/i&gt;=0.035) higher than that for patients without periodontitis, respectively. Trend test showed that the MCI risk increased with periodontitis severity (&lt;i&gt;P&lt;/i&gt;&lt;sub&gt;trend&lt;/sub&gt;=0.016). After adjustments for baseline covariates, multiple linear regression analysis showed that periodontiti","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"43 6","pages":"845-859"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12680121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145663069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Preparation of polycaprolactone-polyethylene glycol-concentrated growth factor composite scaffolds and the effects on the biological properties of human periodontal ligament stem cells].","authors":"Li Gao, Mingyue Zhao, Shun Yang, Runan Wang, Jiajia Cheng, Guangsheng Chen","doi":"10.7518/hxkq.2025.2025044","DOIUrl":"10.7518/hxkq.2025.2025044","url":null,"abstract":"<p><strong>Objectives: </strong>This study investigated the effects of a polycaprolactone (PCL)-polyethylene glycol (PEG) scaffold incorporated with concentrated growth factor (CGF) on the adhesion, proliferation, and osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs).</p><p><strong>Methods: </strong>The PCL-PEG-CGF composite scaffold was fabricated using an immersion and freeze-drying technique. Its microstructure, mechanical properties, and biocompatibility were systematically characterized. The hPDLSCs were isolated through enzymatic digestion, and the hPDLSCs were identified through flow cytometry. Third-passage hPDLSCs were seeded onto the composite scaffolds, and their adhesion, proliferation and osteogenic differentiation were assessed using CCK-8 assays, 4',6-diamidino-2-phenylindole (DAPI) staining, alkaline phosphatase (ALP) staining, alizarin red staining, and Western blot analysis of osteogenesis-related proteins [Runt-related transcription factor 2 (Runx2), ALP, and morphogenetic protein 2 (BMP2)].</p><p><strong>Results: </strong>Scanning electron microscopy revealed that the PCL-PEG-CGF composite scaffold exhibited a honeycomb-like structure with heterogeneous pore sizes. The composite scaffold exhibited excellent hydrophilicity, as evidenced by a contact angle (θ) approaching 0° within 6 s. Its elastic modulus was measured at (4.590 0±0.149 3) MPa, with comparable hydrophilicity, fracture tensile strength, and fracture elongation to PCL-PEG scaffold. The hPDLSCs exhibited significantly improved adhesion to the PCL-PEG-CGF composite scaffold compared with the PCL-PEG scaffold (<i>P</i><0.01). Additionally, cell proliferation was markedly improved in all the experimental groups on days 3, 5, and 7 (<i>P</i><0.01), and statistically significant differences were found between the PCL-PEG-CGF group and other groups (<i>P</i><0.01). The PCL-PEG-CGF group showed significantly elevated ALP activity (<i>P</i><0.05), increased mineralization nodule formation, and upregulated expression of osteogenic-related proteins (Runx2, BMP2 and ALP; <i>P</i><0.05).</p><p><strong>Conclusions: </strong>The PCL-PEG-CGF composite scaffold exhibited excellent mechanical properties and biocompatibility, enhancing the adhesion and proliferation of hPDLSCs and promoting their osteogenic differentiation by upregulating osteogenic-related proteins.</p>","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"43 6","pages":"819-828"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12680126/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145663079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Applications and perspectives of artificial intelligence in periodontology].","authors":"Chunxu Lü, Shaohua Ge","doi":"10.7518/hxkq.2025.2025103","DOIUrl":"10.7518/hxkq.2025.2025103","url":null,"abstract":"<p><p>Artificial intelligence (AI) is rapidly advancing in periodontology, bringing new opportunities to clinical diagnosis, risk assessment, personalized treatment planning, and remote patient care. Leveraging core technologies such as deep learning, machine learning, and natural language processing, AI significantly enhances the sensitivity of early periodontal disease detection and provides precise quantification of alveolar bone loss and soft tissue damage. AI facilitates multimodal data integration by synthesizing medical history, lifestyle factors, and imaging data, thereby offering enhanced accurate risk prediction and personalized therapeutic recommendations. By integrating remote monitoring with tailored health counseling, AI helps patients maintain adherence to self-care protocols, significantly improving their oral health-related quality of life and treatment satisfaction. Moreover, AI demonstrates considerable potential in periodontal research and education, particularly in large-scale data mining, virtual clinical case simulations, and natural language processing-assisted literature management. Nevertheless, challenges remain concerning model generalizability, data quality, ethical concerns, and interpretability. The advancement of multi-center big-data platforms is expected to foster a profound integration of AI and periodontology, propelling precision medicine and digital healthcare, enabling holistic management from prevention to long-term care, and enhancing diagnostic efficiency and patient health outcomes.</p>","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"43 5","pages":"620-627"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12573713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145396106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Metabolic reprogramming by glutathione S-transferase enhances environmental adaptation of <i>Streptococcus mutans</i>].","authors":"Haoyue Zheng, Xian Peng, Jing Zou","doi":"10.7518/hxkq.2025.2025084","DOIUrl":"10.7518/hxkq.2025.2025084","url":null,"abstract":"<p><strong>Objectives: </strong>This study aims to investigate the impact of glutathione S-transferase (GST) on the environmental adaptability of <i>Streptococcus mutans</i> (<i>S. mutans</i>).</p><p><strong>Methods: </strong>A GST knockout strain ΔgsT was constructed. Transcriptomic sequencing was performed to analyze the gene expression differences between the wild-type <i>S. mutans</i> UA159 and its GST knockout strain ΔgsT. Comprehensive functional assessments, including acid tolerance assays, hydrogen peroxide challenge assays, nutrient limitation growth assays, and fluorescence in situ hybridization, were conducted to evaluate the acid tolerance, antioxidant stress resistance, growth kinetics, and interspecies competitive ability of ΔgsT within plaque biofilms.</p><p><strong>Results: </strong>Compared with the wild-type <i>S. mutans</i>, 198 genes in ΔgsT were significantly differentially expressed and enriched in pathways related to metabolism, stress response, and energy homeostasis. The survival rate of ΔgsT in acid tolerance assays was markedly reduced (<i>P</i><0.01). After 15 min of hydrogen peroxide challenge, the survival rate of ΔgsT decreased to 38.12% (wild type, 71.75%). Under nutrient-limiting conditions, ΔgsT exhibited a significantly lower final OD₆₀₀ value than the wild-type strain (<i>P</i><0.05). In the biofilm competition assays, the proportion of <i>S. mutans</i> ΔgsT in the mixed biofilm (8.50%) was significantly lower than that of the wild type (16.89%) (<i>P</i><0.05).</p><p><strong>Conclusions: </strong>GST enhances the acid resistance, oxidative stress tolerance, and nutrient adaptation of <i>S. mutans</i> by regulating metabolism-related and stress response-related genes.</p>","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"43 5","pages":"728-735"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12573710/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145396087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Layered external repair with microsurgery of maxillary central incisor with external cervical resorption].","authors":"Xiao Ji, Lan Zhang, Dingming Huang","doi":"10.7518/hxkq.2025.2025033","DOIUrl":"10.7518/hxkq.2025.2025033","url":null,"abstract":"<p><p>Tooth resorption is the degradation of dental hard tissue due to the continuous action of odontoclasts. External cervical resorption refers to the cervical resorption of a tooth due to destruction and/or defects of the periodontal ligament or subepithelial cementum. In this paper, we report a case of maxillary central incisor with external cervical resorption after dental trauma and orthodontic treatment. The resorption site and morphology of the affected tooth were thoroughly analyzed by cone beam computed tomography prior to the operation. Dental operating microscope and layered external repair with composite resin and bioceramics ensured a perfect restoration of the resorption, and the vital pulp was preserved. The 6-year follow-up indicated a favorable treatment effect.</p>","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"43 5","pages":"736-741"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12573705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145396025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Removal of a fishbone foreign body from the root of the tongue using a translingual ventral approach: case report].","authors":"Chengyan Li, Shuangyuan Zhao, Yi Li, Bo Han, Bowen Zhang","doi":"10.7518/hxkq.2025.2024414","DOIUrl":"10.7518/hxkq.2025.2024414","url":null,"abstract":"<p><p>Foreign bodies in the tongue are rare in clinical practice. Accurate localization and appropriate surgical path selection are essential to reduce surgical risk and postoperative complications. This paper reports a case in which the fishbone foreign body at the base of tongue was removed using a translingual ventral approach aided with imaging localization.</p>","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"43 5","pages":"742-747"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12573708/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145396104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Expression and prognostic value of mothers against decapentaplegic homolog 7 in head and neck squamous cell carcinoma].","authors":"Haihui Zhao, Xiaojuan Zhong, Yi Huang, Wei Fei","doi":"10.7518/hxkq.2025.2024341","DOIUrl":"10.7518/hxkq.2025.2024341","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to explore the biological functions and clinical value of mothers against decapentaplegic homolog (SMAD) 7 in head and neck squamous cell carcinoma (HNSCC) through bioinformatics analysis and basic experiments.</p><p><strong>Methods: </strong>The expression of SMAD7 in HNSCC in public databases was studied. Western blot was used to detect the expression of SMAD7 in HNSCC cell lines and normal epithelial cells. The SMAD7 highly expressed HNSCC cell line HSC-4 was silenced, and CCK-8, Transwell assays, and cell scratch experiments were conducted to study the effect of SMAD7 on the biological functions of HSC-4 cells. HNSCC expression profile data were obtained from UCSC xena, and genes related to SMAD7 were selected for gene ontology and Kyoto encyclopedia of genes and genomes gene enrichment analysis, construction of a co-expression gene interaction network, and screening of related cell signaling pathways. Western blot was used to detect the expression changes of proteins in the related cell signaling pathways in HNSCC cells with silenced SMAD7. cBioPortal was utilized to analyze the mutation rate of the SMAD7 gene, and the MethSurv database was used to analyze the methylation level of the SMAD7 gene and its correlation with prognosis. The receiver operating characteristic curve was used to assess the diagnostic value of SMAD7 for HNSCC. TIMER2.0 was used to analyze the correlation between SMAD7 expression and immune cell infiltration.</p><p><strong>Results: </strong>SMAD7 was highly expressed in HNSCC tumor tissues and some cell lines. Silencing the expression of SMAD7 can significantly inhibit the proliferation, migration, and invasion of cancer cells. Silencing SMAD7 can induce the downregulation of vascular cell adhesion molecule 1 (VCAM-1). The bioinformatics analysis showed that the mutation rate of the SMAD7 gene and the methylation level were significantly correlated with the prognosis of patients with HNSCC. The expression of SMAD7 was related to the level of immune cell infiltration in HNSCC.</p><p><strong>Conclusions: </strong>SMAD7 promotes the proliferation, migration, and invasion of HNSCC cells by regulating the expression of VCAM-1. It may be a potential tumor biomarker and therapeutic target for HNSCC.</p>","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"43 5","pages":"660-670"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12573700/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145396066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Defect modification of calcium silicate and its application in oral bacteriostasis and tooth remineralization].","authors":"Yuanyuan Hu, Shuyan Zhang, Jianhua Zhang, Hongrong Luo, Yunfeng Li, Jing Zhang, Xianchun Chen","doi":"10.7518/hxkq.2025.2025004","DOIUrl":"10.7518/hxkq.2025.2025004","url":null,"abstract":"<p><strong>Objectives: </strong>Calcium silicate (CSO) is modified to give it photothermal antibacterial properties. Its application potential in tooth mineralization and oral antibacterial is evaluated.</p><p><strong>Methods: </strong>Based on defect-engineering modification strategy, a series of CSO-T samples (CSO-300, CSO-400, CSO-500, CSO-600) was obtained by introducing oxygen vacancy into CSO through thermal reduction using sodium borohydride. The samples were tested using scanning electron microscopy (SEM), X-ray diffraction, X-ray photoelectron spectroscopy, ultraviolet near-infrared absorption spectroscopy, and infrared thermography. The powder samples with the best photothermal performance and the most suitable material concentration (CSO-500, 500 μg/mL) were selected for subsequent experiments. High resolution transmission electron microscopy was used to analyze the microstructure and morphology of the sample, and MTT assay and Calcein AM/PI live/dead cell staining were used to evaluate the toxicity and compatibility of the sample to human oral keratinocytes. <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> were selected for photothermal antibacterial experiments to evaluate their <i>in vitro</i> antibacterial performance. SEM, energy dispersive spectrometer, and micro Vickers hardness tester were used to evaluate the ability of materials to induce <i>in vitro</i> remineralization of detached teeth.</p><p><strong>Results: </strong>Oxygen vacancies changed the crystal type and lattice spacing of CaSiO₃, broadened the light-absorption range, and gave it a good photothermal conversion ability in response to near infrared. <i>In</i><i>vitro</i> experiments showed that the modified CaSiO₃ could promote the formation of hydroxyapatite on the tooth surface, thereby promoting the remineralization of teeth and improving the teeth hardness. Moreover, it had photothermal antibacterial properties and no cytotoxicity.</p><p><strong>Conclusions: </strong>Defect-modified black calcium silicate has multiple functions, such as promoting tooth remineralization and photothermal bacteriostatic. When combined with the infrared luminescent toothbrush, it can simply and effectively treat tooth enamel erosion and oral bacteriostatic diseases caused by the excessive consumption of carbonated beverages and other daily bad living habits. This combination is expected to achieve the synergic treatment effect of tooth remineralization and oral bacteriostatic through daily cleaning is expected.</p>","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"43 5","pages":"648-659"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12573702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145396083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}