Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology最新文献

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[Analysis of complicating factors in endodontic microsurgery]. 牙髓显微手术并发症因素分析
Xuelian Tan, Li Peng, Qin Su, Xin Xu, Jinbo Yang, Dongzhe Song, Chenglin Wang, Lan Zhang, Ling Ye, Dingming Huang
{"title":"[Analysis of complicating factors in endodontic microsurgery].","authors":"Xuelian Tan, Li Peng, Qin Su, Xin Xu, Jinbo Yang, Dongzhe Song, Chenglin Wang, Lan Zhang, Ling Ye, Dingming Huang","doi":"10.7518/hxkq.2026119","DOIUrl":"10.7518/hxkq.2026119","url":null,"abstract":"<p><p>Endodontic microsurgery (EMS) can handle challenging endodontic cases effectively. However, the complexity and postoperative outcome of EMS are closely related to multiple factors such as the patient's general condition, the affected tooth, and the surrounding anatomical structures. This article draws on international and domestic medical standards, including the American Society of Anesthesiologists, the American Association of Endodontists, the World Health Organization, and the expert consensus on the assessment of root canal treatment difficulty in China. Based on the basic principles, procedures, and anatomical data of EMS, combined with long-term clinical experience, clinical epidemio-logy, and evidence-based medicine, this article analyzes the difficult and complex factors of EMS cases. This work aims to provide references for treatment decisions, doctor-patient communication, application and promotion, clinical tea-ching, and hierarchical referral in EMS.</p>","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"44 3","pages":"301-310"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13268869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148260913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Considerations for soft tissue management in endodontic microsurgery]. 【根管显微手术中软组织处理的注意事项】。
Jing Huang, Shensheng Gu
{"title":"[Considerations for soft tissue management in endodontic microsurgery].","authors":"Jing Huang, Shensheng Gu","doi":"10.7518/hxkq.2026080","DOIUrl":"10.7518/hxkq.2026080","url":null,"abstract":"<p><p>With the development of microsurgical endodontic techniques, their clinical application is gradually expanding. As a critical component, soft tissue management significantly affects surgical outcomes, especially because many cases involve the aesthetic zone of anterior teeth. Balancing aesthetic and functional restorations aligns with mo-dern dental principles. In this article, the key points in soft tissue management during microsurgical endodontics were systematically outlined, covering flap design principles, preoperative assessment, operative techniques, and the management of a particularly challenging issue, that is, chronic periapical lesions combined with soft tissue fenestration. This work aims to enhance clinicians' understanding of soft tissue handling in microsurgical endodontics and improve clinical treatment standards.</p>","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"44 3","pages":"319-328"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13268855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148260885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Analysis and management of the influence of periapical lesion size on endodontic microsurgery]. 根尖周病变大小对根管显微手术影响的分析与处理
Xiangya Huang, Kai Zeng, Ruiqi Yang, Xi Wei
{"title":"[Analysis and management of the influence of periapical lesion size on endodontic microsurgery].","authors":"Xiangya Huang, Kai Zeng, Ruiqi Yang, Xi Wei","doi":"10.7518/hxkq.2026091","DOIUrl":"10.7518/hxkq.2026091","url":null,"abstract":"<p><p>Endodontic microsurgery is a critical treatment option that is often considered when conventional root canal therapy fails or when complex periapical diseases cannot be resolved through non-surgical treatment. The clinical outcomes of endodontic microsurgery are influenced by multiple factors. Among these, the size of the periapical lesion is considered a key variable that can significantly impact surgical design, operative difficulty, postoperative infection control, and tissue healing. This article systematically discusses the mechanisms by which the size of periapical lesions affects the efficacy of endodontic microsurgery, preoperative assessment methods, intraoperative management strategies, and prognostic correlations. On the basis of existing evidence from evidence-based medicine, clinical management re-commendations are proposed to provide a theoretical foundation and operational guidance for clinical practice.</p>","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"44 3","pages":"329-336"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13268859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148260887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Expert consensus on the "Four-Level and Dual-Axis" comprehensive diagnostic and therapeutic system for prosthetic rehabilitation of oral diseases]. 【口腔疾病修复康复“四级双轴”综合诊疗体系专家共识】。
{"title":"[Expert consensus on the \"Four-Level and Dual-Axis\" comprehensive diagnostic and therapeutic system for prosthetic rehabilitation of oral diseases].","authors":"","doi":"10.7518/hxkq.2026066","DOIUrl":"10.7518/hxkq.2026066","url":null,"abstract":"<p><p>Traditionally, prosthodontics has adhered to a clinical diagnosis and treatment model centered on \"tooth defects, partial edentulism, and complete edentulism\", which show insufficient alignment with the modern \"biopsychosocial\" medical model. The demand of high-quality medical services continues to increase with the nation's growing prosperity and the continuous extension of average life expectancy. Facing these heightened medical demands, particularly the challenges of oral frailty and multimorbidity in an aging society, the traditional diagnostic and therapeutic system for prosthodontics increasingly reveals limitations, including insufficient information coverage, obscuring the underlying etio-logy, the neglect of individual psychological needs, and a lack of holistic consideration for multimorbidity. This consensus proposes the \"Four-Level and Dual-Axis\" comprehensive diagnostic and therapeutic system for prosthetic rehabilitation of oral diseases to address these bottlenecks in disciplinary development. This system classifies the diagnostic scope from micro to macro into four functional levels of \"tooth-pulp\", \"dentition-occlusion\", \"occlusion-temporomandibular joint\", and \"maxillofacial-systemic\" emphasizing the pathological cascade reactions between levels and a bidirectional diagnostic logic. Simultaneously, it introduces a subjective-objective dual-axis assessment model comprising \"individual psychology and prosthetic needs (axis A)\" and \"etiology and patho-anatomical diagnosis (axis B),\" achieving an integration of medical rationality and humanistic care. By reconstructing diagnostic logic and implementing standardized sequential treatment pathways, this consensus aims to facilitate the continuous transition of prosthodontics toward the \"biopsychosocial\" medical model, shifting from a \"prosthesis-centered\" approach to a \"patient-centered and disease-focused\" model of precision personalized prosthetic rehabilitation.</p>","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"44 3","pages":"368-377"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13268854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148260875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Retrospective analysis of localized juvenile spongiotic gingival hyperplasia]. [局限性青少年海绵状牙龈增生回顾性分析]。
Yanju Lin, Rongfang Cao, Rui Yao, Jianwei Shang
{"title":"[Retrospective analysis of localized juvenile spongiotic gingival hyperplasia].","authors":"Yanju Lin, Rongfang Cao, Rui Yao, Jianwei Shang","doi":"10.7518/hxkq.2025.2025176","DOIUrl":"10.7518/hxkq.2025.2025176","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to review and summarize the clinical presentation and pathological features of localized juvenile spongiotic gingival hyperplasia (LJSGH) to enhance pediatric dentists' understanding of this condition.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on the clinical and pathological data of patients with LJSGH diagnosed in the Department of Oral Pathology, Tianjin Stomatological Hospital from January 2019 to December 2024.</p><p><strong>Results: </strong>Twelve cases were retrieved, the male-to-female ratio was 1.4∶1, and nine of the cases were children and adolescents (<18 years old). The typical clinical presentation was characterized by erythematous, hyperplastic gingiva exhibiting a papillary or granular appearance. The lesion was predominantly solitary and primarily located on the labial gingiva of the maxillary anterior region. The typical histopathological features were as follows: the superficial epithelium of the lesional area consisted of nonkeratinized stratified squamous epithelium with intercellular edema and spongiosis; irregular elongation of rete ridges; neutrophil infiltration in the superficial epithelial layer; marked vascular dilation and congestion in the subepithelial papillary layer; and a distinct boundary between the lesional epithelium and adjacent normal mucosal epithelium. CK19 exhibited strong positive expression throughout the lesion epithelium. By contrast, only the basal layer showed a strong positive expression in normal gingival tissues.</p><p><strong>Conclusions: </strong>LJSGH exhibits distinctive clinical features, and its diagnosis can be confirmed through histopathological examination. The fin-dings of this study contribute to enhancing pediatric dentists' understanding and recognition of LJSGH.</p>","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"44 2","pages":"259-265"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13047890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147597126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Clinical symptoms and cone beam computed tomography imaging analysis of patients with temporomandibular joint osteoarthritis with chewing side preference before and after treatment of stabilization splint]. [稳定夹板治疗伴有咀嚼侧偏的颞下颌关节骨性关节炎患者的临床症状及锥形束计算机断层成像分析]。
Xiaoxing Li, Jiazhu Wang, Laiqing Xu, Xinyu Xu, Hongbo Li, Min Hu, Hongchen Liu, Hua Jiang
{"title":"[Clinical symptoms and cone beam computed tomography imaging analysis of patients with temporomandibular joint osteoarthritis with chewing side preference before and after treatment of stabilization splint].","authors":"Xiaoxing Li, Jiazhu Wang, Laiqing Xu, Xinyu Xu, Hongbo Li, Min Hu, Hongchen Liu, Hua Jiang","doi":"10.7518/hxkq.2026.2025360","DOIUrl":"10.7518/hxkq.2026.2025360","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;This study aimed to explore the clinical efficacy of stabilization splint (SS) and the characteristics of cone beam computed tomography (CBCT) imaging changes by analyzing the CBCT images and clinical symptoms of patients with temporomandibular joint osteoarthritis (TMJOA) complicated with chewing side preference before and after treatment with SS. Me⁃thods A retrospective analysis was conducted on 74 TMJOA patients (aged 18-40 years) who visited the Department of Stomatology, First Medical Center, Chinese PLA General Hospital from June 2021 to January 2025. Among them, 31 patients had no chewing side preference (mean age: 29.81±2.99 years), and 43 patients had chewing side preference (mean age: 30.88±5.65 years). The CBCT imaging features and clinical symptoms of the two groups at the initial visit and follow-up (6 months later) were observed and analyzed. SPSS 27.0 software was used for data analysis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Comparison of patients without chewing side preference before and after SS treatment: the incidence of pain decreased from 100% to 3.23%, limited mouth opening from 96.77% to 3.23%, and joint noise from 80.65% to 38.71%, with statistically significant differences (&lt;i&gt;P&lt;/i&gt;&lt;0.05). CBCT showed that the ipsilateral posterior joint space and superior joint space increased significantly (&lt;i&gt;P&lt;/i&gt;&lt;0.05), whereas no significant differences were observed in the temporomandibular joint (TMJ) indices between the two sides (&lt;i&gt;P&lt;/i&gt;&gt;0.05). Comparison of patients with chewing side preference before and after SS treatment: the incidence of pain decreased from 100% to 4.65%, limited mouth opening from 88.37% to 2.33%, and joint noise from 79.07% to 39.53%, with statistically significant differences (&lt;i&gt;P&lt;/i&gt;&lt;0.05). CBCT showed that the medial-lateral diameter of the condyle, anterior-posterior diameter of the condyle, intra-articular space, posterior joint space, superior joint space, slope of the articular eminence, and condylar height on the habitual chewing side increased significantly (&lt;i&gt;P&lt;/i&gt;&lt;0.05). Moreover, the medial-lateral diameter of the condyle, anterior-posterior diameter of the condyle, intra-articular space, posterior joint space, and superior joint space on the non-habitual chewing side increased significantly (&lt;i&gt;P&lt;/i&gt;&lt;0.05). The posterior joint space, intra-articular space, and condylar height on the habitual chewing side were significantly larger than those on the non-habitual chewing side (&lt;i&gt;P&lt;/i&gt;&lt;0.05). Comparison between patients with and without chewing side preference after SS treatment: no significant differences were found in pain, limited mouth opening, and joint noise between the two groups (&lt;i&gt;P&lt;/i&gt;&gt;0.05).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;SS is effective in treating patients suffering from TMJOA with or without chewing side preference because it can effectively relieve joint pain, significantly improve mouth opening, and reduce the incidence of joint noise to a cer","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"44 2","pages":"266-276"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13047861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147597181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Clinical efficacy and prognostic factors of pulpotomy combined with fragment reattachment in treating complicated crown-root fractures of maxillary young permanent anterior teeth]. [截髓联合碎片再附着治疗上颌年轻恒前牙复杂冠根骨折的临床疗效及影响因素分析]。
Xin Yang, Yuanyuan Wang, Chanchan Chen, Guicong Ding, Xinmin Liao, Zhiqing Liao
{"title":"[Clinical efficacy and prognostic factors of pulpotomy combined with fragment reattachment in treating complicated crown-root fractures of maxillary young permanent anterior teeth].","authors":"Xin Yang, Yuanyuan Wang, Chanchan Chen, Guicong Ding, Xinmin Liao, Zhiqing Liao","doi":"10.7518/hxkq.2025.2025130","DOIUrl":"10.7518/hxkq.2025.2025130","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to evaluate the clinical efficacy of pulpotomy combined with fragment reattachment in treating complicated crown-root fractures of maxillary young permanent anterior teeth.</p><p><strong>Methods: </strong>A retrospective analysis of 37 cases of complicated crown-root fractures treated at Shenzhen Children's Hospital from January 2019 to December 2023 was conducted. Outcomes such as fragment retention rate, pulp survival rate, root development, and periodontal health were assessed, and the influencing factors were analyzed.</p><p><strong>Results: </strong>The fragment retention rates were 80.9% at 1 year and 69.5% at 2 years. The remaining crown height (cervical half group vs. incisal half group) emerged as a critical predictor of retention (<i>P</i><0.05, hazard ratio=7.45, 95% confidence interval: 1.28-43.48). The pulp survival rate reached 91.6% at 1 year, with no significant influencing factors identified. In cases where the fractured crown retained for over one year, teeth at Nolla stage 8 showed significantly greater root elongation and apical foramen narrowing compared to those at Nolla stage 9 (<i>P</i><0.05), normal periodontal scores were observed in 59.3%.</p><p><strong>Conclusions: </strong>Pulpotomy combined with fragment reattachment effectively preserves pulp vitality and restores dental function and morphology in young permanent anterior teeth. The remaining crown height serves as a key prognostic indicator for fragment retention. This approach also promotes root development and demonstrates favorable clinical outcomes.</p>","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"44 2","pages":"188-196"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13047871/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147597011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Comparative analysis of microbial communities in different oral ecological niches of preschool children]. 学龄前儿童口腔不同生态位微生物群落比较分析
Jiajia Liu, Yuhui Sun, Juefei Wu, Shuai Tang, Gang Ding
{"title":"[Comparative analysis of microbial communities in different oral ecological niches of preschool children].","authors":"Jiajia Liu, Yuhui Sun, Juefei Wu, Shuai Tang, Gang Ding","doi":"10.7518/hxkq.2025.2025285","DOIUrl":"10.7518/hxkq.2025.2025285","url":null,"abstract":"<p><strong>Objectives: </strong>This study aims to investigate the structural differences, dominant bacterial genera, and potential functions of microbial communities in different oral ecological niches (dorsal tongue, tooth surface, and buccal mucosa) of preschool children to clarify the influence of local microenvironments on microbial colonization and provide a theoretical basis for the microbiota-targeted regulation of pediatric oral diseases.</p><p><strong>Methods: </strong>A total of 105 plaque samples were collected from the dorsal tongue, tooth surface, and buccal mucosa of 35 healthy preschool children (aged 4-6 years). High-throughput sequencing of the 16S rRNA gene was performed to analyze the microbial community structure and alpha/beta diversity. Principal coordinate analysis, UPGMA clustering, and LEfSe analysis were used to identify niche-specific dominant genera. PICRUSt2 was applied to predict the potential metabolic functional profiles across niches.</p><p><strong>Results: </strong>Spatial hete-rogeneity in microbial composition and structure was observed across the three oral niches. Species richness was significantly higher on the tooth surface and buccal mucosa than on the dorsal tongue. The dominant phyla included Firmicutes, Actinobacteria, Proteobacteria, Bacteroidetes, Fusobacteria, and Patescibacteria. At the genus level, Actinomyces and Corynebacterium were enriched on the tooth surface, Veillonella was enriched on the dorsal tongue, and Streptococcus was predominant on the buccal mucosa. Beta diversity and clustering analyses confirmed distinct microbial community structures among the niches. LEfSe analysis identified several niche-specific genera. PICRUSt2 functional prediction revealed significant differences in amino acid metabolism, carbohydrate metabolism, cell motility, translation, signal transduction, immune system, infectious disea-ses, and membrane transport (<i>P</i><0.05).</p><p><strong>Conclusions: </strong>The dorsal tongue, tooth surface, and buccal mucosa in preschool children harbor distinct microbial communities with different taxonomic composition, diversity, and functional potential. The findings suggest that local microenvironmental factors shape oral microbiota and may contribute to the early onset of oral diseases. This study provides foundational data and theoretical insights for early microbiome-based risk assessment and personalized oral health interventions in children.</p>","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"44 2","pages":"197-205"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13047870/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147597130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Dihydroartemisinin inhibits the progression of oral squamous cell carcinoma]. [双氢青蒿素抑制口腔鳞状细胞癌的进展]。
Tianyu Shan, Jiajia Liu, Tangjun Liu, Dandan Sun, Xinwei Wang, Yunxia Liu
{"title":"[Dihydroartemisinin inhibits the progression of oral squamous cell carcinoma].","authors":"Tianyu Shan, Jiajia Liu, Tangjun Liu, Dandan Sun, Xinwei Wang, Yunxia Liu","doi":"10.7518/hxkq.2026.2025163","DOIUrl":"10.7518/hxkq.2026.2025163","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to explore the impact of dihydroartemisinin (DHA) on cell proliferation, migration, and invasion in oral squamous cell carcinoma (OSCC). Our findings offer a theoretical foundation for advancing the research and development of novel therapeutic agents for OSCC.</p><p><strong>Methods: </strong>Bioinformatics analysis was conducted using the cancer genome database to classify OSCC patients based on the presence or absence of extracapsular spread and metastasis in tumor tissues, and to compare the differences in survival rates among different OSCC cell lines. The cell proliferation of different OSCC cell lines treated with different DHA concentrations was detected by CCK-8 cytoto-xicity assay, and the optimal drug concentration was screened. The CCK-8 cytotoxicity assay was used to detect the cell proliferation of different OSCC cell lines (CAL27, HN30, and SCC9) after treatment with different concentrations of DHA, and to screen out the optimal drug concentration. The effects of DHA on the proliferation of OSCC cells were detected by CCK-8 cell proliferation assay and cell cloning assay. The effect of DHA on the migration and invasion ability of tumor cells was detected by scratch test and Transwell test. A nude mouse subcutaneous tumor model was constructed to study the effects of DHA on OSCC tumor tissues and major organ.</p><p><strong>Results: </strong>Bioinforma-tics analysis showed that the survival rate of patients with extracapsular spread and metastasis in tumor tissues was lower than that of patients without such phenomena (<i>P</i><0.05). According to CCK-8 cytotoxicity results, the maximum safe concentrations for different OSCC cell lines CAL27, HN30, and SCC9 were 20, 10, and 5 μmol/L, respectively. Proliferation and cloning experiments of CCK-8 cells showed that DHA could inhibit the proliferation of three squamous cell lines (<i>P</i><0.05). Scratch test and Transwell test showed that the migration and invasion levels of CAL27, HN30, and SCC9 cells were significantly decreased under DHA treatment (<i>P</i><0.05). <i>In vivo</i> experiments showed that the growth rate of tumor tissue significantly decreased after DHA treatment (<i>P</i><0.05).</p><p><strong>Conclusions: </strong>DHA exhibits significant inhibitory effects on the proliferation, migration, and invasion of various OSCC cells and growth of tumor tissues.</p>","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"44 2","pages":"224-231"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13047856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147597176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Surgical robot-assisted submandibular gland excision through retroauricular hairline approach: three case reports]. 机器人辅助下经耳后发际入路颌下腺切除3例报告。
Shaofei Ma, Jialu He, Chang Cao, Xiaoyi Wang, Chunjie Li, Guiquan Zhu
{"title":"[Surgical robot-assisted submandibular gland excision through retroauricular hairline approach: three case reports].","authors":"Shaofei Ma, Jialu He, Chang Cao, Xiaoyi Wang, Chunjie Li, Guiquan Zhu","doi":"10.7518/hxkq.2026.2025190","DOIUrl":"10.7518/hxkq.2026.2025190","url":null,"abstract":"<p><p>Conventional submandibular gland resection is typically performed via a lateral cervical open approach, which inevitably leaves a visible scar. As a leading advancement in minimally invasive techniques, robotic-assisted surgery shows significant potential for clinical application. To date, only a limited number of robot-assisted submandibular gland resections have been reported globally, and most were conducted using the Da Vinci Surgical System. No cases involving a domestically developed Chinese robotic system have been documented in either domestic or international lite-rature. Using nearly 100 cases of endoscopic submandibular gland resection through retroauricular hairline approach as basis, we successfully performed three cases of submandibular gland resection with the assistance of a domestically developed multi-arm universal surgical robot. All procedures were completed without intraoperative conversion. Postoperative recovery was uneventful, with no complications including facial nerve palsy, tongue numbness, or impaired tongue mobility. The incisions were well concealed, achieving satisfactory aesthetic outcomes. This preliminary study demonstrates the feasibility of utilizing a Chinese-developed robotic system for submandibular gland resection.</p>","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"44 2","pages":"286-292"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13047885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147597145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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