Comparative biochemistry and physiology. Part D, Genomics & proteomics最新文献

{"title":"Comparative identification, characterization and high-temperature stress analysis of the fatty acid-binding protein (fabp) genes in largemouth bass (Micropterus salmoides) genome.","authors":"Bowen Lv, Yanpeng Liu, Shuai Li, Debin Zhong, Haolin Mo, Lixin Wang, Jiajia Yu","doi":"10.1016/j.cbd.2025.101646","DOIUrl":"https://doi.org/10.1016/j.cbd.2025.101646","url":null,"abstract":"<p><p>Fatty acid binding proteins (Fabps) could participate in multiple biological processes in organisms, such as immune defense, growth, development, metabolism, and stress tolerance. Although the roles of Fabps are well studied, little is known about the stress-induced changes in the Fabp family in largemouth bass. According to genomic analysis, 10 Fabps were detected in the largemouth bass. Phylogenetic and evolutionary analyses suggested that all Fabps could be divided into four clades. According to phylogenetic tree and conserved motif analysis, Fabps were relatively conserved. Real-time quantitative PCR results indicated that 10 fabps showed distinctive expression features in seven tissues, and the transcripts level of 8 fabps in fish liver exhibited marked changes after high-temperature stress. Additionally, a protein-protein interaction analysis revealed that Fabps might participate in responding to high-temperature stress by affecting lipid contents. This study may be the first systematic investigation about Fabps in largemouth bass. It will further deepen our insights into evolutionary processes and provide a critical framework for uncovering the underlying mechanism of Fabps in largemouth bass exposed to high temperature.</p>","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"56 ","pages":"101646"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145245952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolutionary analysis of ghrelin in Actinopterygii.","authors":"Zhuoxin Lai, Hamad Khan, Lujun Chen, Jiahao Luo, Ming Li, Yusong Guo, Zhongduo Wang","doi":"10.1016/j.cbd.2025.101599","DOIUrl":"https://doi.org/10.1016/j.cbd.2025.101599","url":null,"abstract":"<p><p>Ghrelin functions to stimulate appetite, promote the release of growth hormone, and regulate energy balance. Currently, research on the ghrelin is primarily focused on a single species, and there have been no systematic studies on the evolution of the ghrelin in fish. Therefore, this thesis conducts a comprehensive analysis of the ghrelin gene in 151 species of ray-finned bony fishes to reveal the universality and specificity of the ghrelin gene in the evolutionary history of fish, supplementing and perfecting the information on the ghrelin gene in Actinopterygii. The gene identification results show that the number of ghrelin genes varies among different fish species, 41 fish have lost the ghrelin gene, 98 fish having one ghrelin gene, and 12 fish having two ghrelin genes. Among the 110 fish species with the ghrelin gene, a total of 182 ghrelin gene sequences were identified, with transcript variant numbers ranging from 1 to 6, encoding 1 to 3 types of isoform proteins, and their mature peptides show a certain degree of similarity across different species. Phylogenetic analysis revealed that teleost ghrelin proteins segregate into three major evolutionary clades, with Salmoniformes orthologs comprising a distinct monophyletic cluster. The Cladistic and Chondrostei are clustered separately and then grouped with the more ancient Cypriniformes and Siluriformes species from the Neopterygii into a large group, while the other fish species from the Neopterygii form another large group. The Synteny analysis results indicate that the upstream gene of the ghrelin is CCDC174, and the downstream gene is TATDN2. The selection pressure analysis results show that there are no positive selection sites in the ghrelin gene, indicating that the ghrelin has been under strong functional constraint during the evolutionary process of fish. This study systematically investigates the evolutionary history of fish ghrelin, providing a theoretical basis for understanding the function and status of ghrelin in the feeding regulation system of fish, and deepening the recognition of its structural and functional evolution in the process of energy metabolism evolution.</p>","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"56 ","pages":"101599"},"PeriodicalIF":2.4,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144818622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of low temperature on intestinal microbiota composition of silver pomfret (Pampus argenteus).","authors":"Man Zhang, Wenhao Nie, Mengke Tang, Yubei Qiu, Youyi Zhang, Yaya Li, Shangliang Xu, Xiaojun Yan, Shaosen Yang, Jiabao Hu, Yajun Wang","doi":"10.1016/j.cbd.2025.101598","DOIUrl":"https://doi.org/10.1016/j.cbd.2025.101598","url":null,"abstract":"<p><p>Fish are poikilothermic organisms with species-specific optimal temperature ranges for survival. As an economically important marine fish in China, silver pomfret (Pampus argenteus) has overcome artificial breeding bottlenecks. To meet aquaculture requirements in northern regions, we simulated gradual winter cooling from 18 °C to 6 °C using recirculating aquaculture systems with precision temperature control. Through histological sectioning, 16S rRNA gene sequencing, and ecological community assembly modeling, we found that feeding activity in silver pomfret is positively correlated with water temperature, exhibiting a significant decrease with declining thermal conditions. Concurrently, intestinal structural alterations were observed, including diminished collagen staining, enlarged intercellular spaces, and reduced fold height. The microbial diversity initially decreased and subsequently increased during the cooling process. The microbial community was influenced by variable selection and dispersal limitation. At the onset of the cooling period, Vibrio emerged as the dominant genus; however, after prolonged exposure to low-temperature stress, Pseudoalteromonas, Pseudomonas, and Shewanella became predominant genera. Under low temperature conditions, metabolism-related pathways are activated. This study provides new insights into mechanisms underlying low-temperature adaptation and supports silver pomfret breeding at reduced temperatures.</p>","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"56 ","pages":"101598"},"PeriodicalIF":2.4,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144812798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis of Chinese sucker (Myxocyprinus asiaticus) head kidney and discovery of key immune-related genes to cold stress after swimming fatigue.","authors":"Jing Xu, Chuan-Jie Qin, Jiang Xie, Jun Wang, Yang He, Junjun Tan, Xiaotao Shi","doi":"10.2139/ssrn.4379347","DOIUrl":"https://doi.org/10.2139/ssrn.4379347","url":null,"abstract":"For Chinese sucker (Myxocyprinus asiaticus), passing through a dam with fast flow and cold water are always unavoidable, and this process can cause stress, disease or even death. In this study, comparative transcriptome analysis was conducted to investigate the potential immune mechanism in head kidney of M. asiaticus with swimming fatigue stress and cold stress after fatigue. In general, a total of 181,781 unigenes were generated, and 38,545 differentially expressed genes (DEGs) were identified. In these DEGs, 22,593, 7286 and 8666 DEGs were identified among groups of fatigue vs. cold, control vs. cold, and control vs. fatigue, respectively. Enrichment analysis revealed these DEGs were involved in coagulation cascades and complement, natural killer cell mediated cytotoxicity, antigen processing and presentation, Toll-like receptor signaling pathways, and chemokine signaling pathway. Notably, immune genes including heat shock protein 4a (HSP4a), HSP70 and HSP90α genes were significantly up-regulated in fishes with cold stress after fatigue. Differently, more immune genes in control vs. cold compared with that in control vs. fatigue were significantly down-regulated expression, such as claudin-15-like, Toll-like receptor 13, antimicrobial peptide (hepcidin), immunoglobulin, CXCR4 chemokine receptor, T-cell receptor, complement factor B/C2-A3, and interleukin 8. In this study, the number of DEGs in the head kidney was less than that our previous study in the spleen, which we speculated was more sensitive to changes in water temperature than the head kidney. In summary, lots of immune-related genes in the head kidney were down-regulated under cold stress after fatigue, suggesting that M. asiaticus might have experienced severe immunosuppression in the process of passing through the dam.","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"68 1","pages":"101104"},"PeriodicalIF":0.0,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85573899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex matters: Gamete-specific contribution of microRNA following parental exposure to hypoxia in zebrafish.","authors":"W. Heinrichs-Caldas, H. Ikert, V. M. Almeida-Val, P. M. Craig","doi":"10.2139/ssrn.4341895","DOIUrl":"https://doi.org/10.2139/ssrn.4341895","url":null,"abstract":"Oxygen availability varies among aquatic environments, and oxygen concentration has been demonstrated to drive behavioral, metabolic, and genetic adaptations in numerous aquatic species. MicroRNAs (miRNAs) are epigenetic modulators that act at the interface of the environment and the transcriptome and are known to drive plastic responses following environmental stressors. An area of miRNA that has remained underexplored is the sex specific action of miRNAs following hypoxia exposure and its effects as gene expression regulator in fishes. This study aimed to identify differences in mRNA and miRNA expression in the F1 generation of zebrafish (Danio rerio) at 1 hpf after either F0 parental male or female were exposed to 2 weeks of continuous (45 %) hypoxia. In general, F1 embryos at 1 hpf demonstrated differences in mRNA and miRNAs expression related to the stressor and to the specific sex of the F0 that was exposed to hypoxia. Bioinformatic pathway analysis of predicted miRNA:mRNA relationships indicated responses in known hypoxia signaling and mitochondrial bioenergetic pathways. This research demonstrates the importance of examining the specific male and female contributions to phenotypic variation in subsequent generations and provides evidence that there is both maternal and paternal contribution of miRNA through eggs and sperm.","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"20 1","pages":"101090"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87069930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Hermetia illucens larvae meal on the Pacific white shrimp (Litopenaeus vannamei) revealed by innate immunity and 16S rRNA gene sequencing analysis.","authors":"Yongkang Chen, Zhenxiao Zhuang, Jieping Liu, Ziqiao Wang, Yucai Guo, Anqi Chen, Baoyang Chen, Wei Zhao, J. Niu","doi":"10.2139/ssrn.4372700","DOIUrl":"https://doi.org/10.2139/ssrn.4372700","url":null,"abstract":"The larvae of the black soldier fly, Hermetia illucens, are now attracting attention and becoming promising sources for aquafeed ingredient due to the nutritious substance. However, the introduction of a novel ingredient into the recipe may have unpredictable effects on the innate immune function and gut bacteria composition of crustaceans. Therefore, the present study aimed to evaluate how dietary black soldier fly larvae meal (BSFLM) affected the antioxidant ability, innate immunity and gut microbiome of shrimp (Litopenaeus vannamei) fed with a practical diet, including the gene expression of Toll and immunodeficiency (IMD) pathways. Six experimental diets were formulated by replacing gradient levels of fish meal (0 %, 10 %, 20 %, 30 %, 40 % and 50 %) based on a commercial shrimp diet. Four replicates of shrimp were fed different diets three times daily for 60 days. Growth performance linearly decreased with increasing BSFLM inclusion. Results of antioxidative enzyme activities and gene expression suggested that low dietary BSFLM levels activated the antioxidant capacity of shrimp, while dietary BSFLM levels up to 100 g/kg may induce oxidative stress and inhibit glutathione peroxidase activity. Although traf6, toll1, dorsal and relish were significantly upregulated in different BSFLM groups, the expression of tak1 was significantly downregulated in groups containing BSFLM, implying the immune susceptibility may be weakened. Gut flora analysis indicated dietary BSFLM altered both beneficial and opportunistic pathogenic bacterial abundance, with low levels of dietary BSFLM increased the abundance of bacteria that may contribute to carbohydrate utilization, while high levels of dietary BSFLM may cause intestinal disease and low intestinal immune response. To conclude, 60-80 g/kg of dietary BSFLM showed no adverse effects on the growth, antioxidant capacity and gut flora of shrimp, which was the adequate level in shrimp diet. While 100 g/kg dietary BSFLM may induce oxidative stress and potentially weaken the innate immunity of shrimp.","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"1 1","pages":"101080"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84394781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HPLC-MS-based untargeted metabolomic analysis of differential plasma metabolites and their associated metabolic pathways in reproductively anosmic black porgy, Acanthopagrus schlegelii.","authors":"Xing Shen, Xian Li, Chaofeng Jia, Jun Li, Shuyin Chen, Bo Gao, Wen-Zhi Liang, Libin Zhang","doi":"10.2139/ssrn.4330797","DOIUrl":"https://doi.org/10.2139/ssrn.4330797","url":null,"abstract":"Olfaction, a universal form of chemical communication, is a powerful channel for animals to obtain social and environmental cues. The mechanisms by which fish olfaction affects reproduction, breeding and disease control are not yet clear. To evaluate metabolites profiles, plasma from anosmic and control black porgy during reproduction was analyzed by non-targeted metabolomics using ultra high-performance liquid chromatography-mass spectrometry and multivariate statistical analysis techniques, including principal component analysis and orthogonal partial least squares discriminant analysis. The metabolite profiles of anosmia and control groups were found to be significantly separated. Ten different differential metabolites, mainly including amino acids, such as isoleucine and methionine, and lipids, such as phosphatidylserine, were screened based on the combined analysis of variable importance in the projection and p values. In addition, six key differential metabolic pathways were analyzed using the Kyoto Encyclopedia of Genes and Genomes and enriched for four metabolic pathways including the citrate acid (TCA) cycle, tyrosine metabolism, arginine and proline metabolism, and arginine synthesis. The TCA cycle enhances fertility through the reduction of pyruvate kinase, and intermediate derivatives (acetyl CoA, malonyl CoA) act as signaling factors that regulate immune cell function. The tyrosine cycle can indirectly participate and promote reproduction in black porgy through melanin-concentrating hormone. Arginine and proline metabolism can promote reproduction by promoting growth hormone and enhance immunity in anosmic black porgy by stimulating T lymphocytes. Our metabolomic study revealed that anosmia in black porgy played an active role in immunity and reproduction and provided theoretical support for breeding and disease control.","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"29 1","pages":"101071"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82442165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fatter or stronger: Resource allocation strategy and the underlying metabolic mechanisms in amphibian tadpoles.","authors":"Wei Zhu, Liming Chang, Guocheng Shu, Bin Wang, Jianping Jiang","doi":"10.21203/rs.3.rs-77495/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-77495/v1","url":null,"abstract":"The allocation of resources between storage and somatic growth is an essential physiological phenomenon in animals. Allocation mechanisms have broad theoretical and applied implications. The real-time resource allocation patterns in animals remain to be elucidated, and there is limited understanding of the metabolic mechanisms. We investigated the resource allocation strategy of Rana omeimontis tadpoles. Their ontogenetic fat accumulation began when body weight increased to 30-50 mg, at which time storage had a high priority in resource allocation. Beyond this weight range, somatic growth accelerated but storage investment was maintained, resulting in a positive correlation between body fat index and body weight at the population level. This pattern could be explained by assuming a positive relationship between storage abundance and growth investment, and this was supported by the prioritized increment of body fat to body weight when tadpoles were provided with increased food. At the metabolic level, hepatic fat accumulation was accompanied by upregulated utilization of fat storage, and the tadpoles presented lipid-based energy metabolism. Activating the mobilization of hepatic fat storage promoted somatic growth. In short, the liver is like a reservoir with valves that regulate energy flow for downstream developmental processes. These results provide novel mechanistic insights into resource allocation.","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"1 1","pages":"100825"},"PeriodicalIF":0.0,"publicationDate":"2020-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89867398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and preliminary characterization of chemosensory-related proteins in the gall fly, Procecidochares utilis by transcriptomic analysis.","authors":"Lifang Li, Xi Gao, Huamin Gui, Mingxian Lan, Jiaying Zhu, Yong-hui Xie, Youguo Zhan, Zhi-jiang Wang, Li Zhengyue, M. Ye, Guo-xing Wu","doi":"10.21203/rs.3.rs-18943/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-18943/v1","url":null,"abstract":"Chemoreception is critical for insect behaviors such as foraging, host searching and oviposition. The process of chemoreception is mediated by a series of proteins, including odorant-binding proteins (OBPs), gustatory receptors (GRs), odorant receptors (ORs), ionotropic receptors (IRs), chemosensory proteins (CSPs) and sensory neuron membrane proteins (SNMPs). The tephritid stem gall fly, Procecidochares utilis Stone, is a type of egg parasitic insect, which is an effective biological control agent for the invasive weed Ageratina adenophora in many countries. However, the study of molecular components related to the olfactory system of P. utilis has not been investigated. Here, we conducted the developmental transcriptome (egg, first-third instar larva, pupa, female and male adult) of P. utilis using next-generation sequencing technology and identified a total of 133 chemosensory genes, including 40 OBPs, 29 GRs, 24 ORs, 28 IRs, 6 CSPs, and 6 SNMPs. The sequences of these candidate chemosensory genes were confirmed by BLAST, and phylogenetic analysis was performed. Quantitative real-time PCR (qRT-PCR) confirmed that the expression levels of the candidate OBPs varied at the different developmental stages of P. utilis with most OBPs expressed mainly in the pupae, female and male adults but scarcely in eggs and larvae, which was consistent with the differentially expressed genes (DEGs) analysis using the fragments per kilobase per million fragments (FPKM) value. Our results provide a significant contribution towards the knowledge of the set of chemosensory proteins and help advance the use of P. utilis as biological control agents.","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"29 1","pages":"100724"},"PeriodicalIF":0.0,"publicationDate":"2020-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87664216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of gonadotropin-releasing hormone analog (GnRHa) immunization on the gonadal transcriptome and proteome of tilapia (Oreochromis niloticus).","authors":"Rui Wang, Luting Wen, Huawei Ma, Min Lv, Zhong Chen, Xuesong Du, Yong Lin, Huizan Yang","doi":"10.21203/rs.3.rs-18227/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-18227/v1","url":null,"abstract":"Gonadotropin releasing hormone (GnRH) plays an important role in the regulation of vertebrate reproduction. Studies have shown that immunization against GnRHa can induce sexually sterile tilapia. To explore the mechanism behind this, in this study, RNA-seq and data-independent acquisition (DIA) techniques were used to study the transcriptome and proteome of the gonad of tilapia immunized with GnRHa. 644 differentially expressed genes (80 upregulated and 564 downregulated) and 1150 differentially expressed proteins (351 upregulated and 799 downregulated) were identified. There were 209 genes with consistent differential expression patterns in the transcriptomic and proteomic analyses, of which 9 were upregulated and 200 downregulated, indicating that the gonad gene expression was inhibited by GnRHa immunization. The downregulated genes were particularly involved in the functions of single-organism process, binding, cellular process, metabolic process and catalytic activity, and associated with the pathways including ECM-receptor interaction, focal adhesion, cardiac muscle contraction and oxidative phosphorylation. The expression of six differentially expressed genes involved in the GnRH signaling pathway was all downregulated. In addition, several important functional genes related to gonadal development after GnRHa immunization were screened. This study confirmed the expression of corresponding genes was affected by GnRHa on the gonad development in tilapia at the molecular level, and laid a foundation for elucidating the mechanism of GnRHa immunization.","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"52 1","pages":"100780"},"PeriodicalIF":0.0,"publicationDate":"2020-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88945122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}