Comparative biochemistry and physiology. Part D, Genomics & proteomics最新文献

{"title":"Transcriptomic and RNAi analyses reveal chloride channel 3-associated osmoregulation in Litopenaeus vannamei under low-salinity stress.","authors":"Ti Wang, Qijian Zhu, Xuenan Li, Yun Li","doi":"10.1016/j.cbd.2026.101987","DOIUrl":"10.1016/j.cbd.2026.101987","url":null,"abstract":"<p><p>Chloride channels and transporters are important for cellular volume regulation and salinity adaptation in euryhaline crustaceans, yet the intestinal transcriptional relationship between plasma-membrane and intracellular chloride pathways remains unclear in Litopenaeus vannamei. In this study, RNA interference of anoctamin 1 (ANO1) was combined with intestinal transcriptome sequencing under the production-relevant low-salinity condition of salinity 3. ANO1 silencing produced a focused transcriptional response, with 16 differentially expressed genes (DEGs) identified (11 upregulated and 5 downregulated). Functional enrichment indicated that these genes were associated with transporter activity, cytoskeletal organization, extracellular matrix-receptor interaction, membrane lipid metabolism, and vesicular processes. Notably, a transcript encoding chloride channel protein 3 (CLC-3) was significantly upregulated following ANO1 knockdown, suggesting a potential transcriptional relationship between ANO1 and CLC-3 in chloride homeostasis. Based on this finding, CLC-3 was selected for full-length cDNA cloning, sequence characterization, salinity-gradient expression analysis, and RNAi-based functional assessment. The cloned CLC-3 cDNA was 2883 bp in length and encoded an 850 amino acid protein containing a conserved voltage-gated chloride channel (Voltage-CLC) domain and two cystathionine β-synthase domains. Phylogenetic analysis placed LvCLC-3 within the intracellular CLC-c clade, and tissue distribution analysis showed the highest CLC-3 expression in the intestine. Intestinal CLC-3 expression responded nonlinearly to salinity variation, peaking at salinity 20. Under salinity 3, CLC-3 knockdown reduced ANO1, Na⁺/K⁺-ATPase alpha subunit, and Na⁺-K⁺-2Cl^- cotransporter transcript levels, whereas glutamate-gated chloride channel expression increased. Mild hepatopancreatic structural alterations were also observed after CLC-3 knockdown. These findings suggest that CLC-3 is a salinity-responsive intracellular chloride-transporter candidate associated with intestinal ion-transport-related transcriptional responses after ANO1 suppression in L. vannamei, although the underlying physiological mechanism requires further validation.</p>","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"60 ","pages":"101987"},"PeriodicalIF":2.4,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Characteristics of the long non-coding RNA-mRNA regulatory network in American eel (Anguilla rostrata) during Vibrio harveyi infection\" [Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics 59 (2026) 101871].","authors":"Guanghua Sun, Liqun Wu, Yijun Tang, Sijia Hong, Songlin Guo","doi":"10.1016/j.cbd.2026.101884","DOIUrl":"10.1016/j.cbd.2026.101884","url":null,"abstract":"","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":" ","pages":"101884"},"PeriodicalIF":2.4,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148058931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The immune enhancement strategies of Strongylocentrotus intermedius in response to different immunomodulators.","authors":"Yaqiong Liu, Yulu Jiang, Nan Gao, Sifan Chen, Wenhao Yang, Jinhai Zhang, Yijing Han, Xiaotong Wang","doi":"10.1016/j.cbd.2026.102057","DOIUrl":"https://doi.org/10.1016/j.cbd.2026.102057","url":null,"abstract":"<p><p>The sea urchin Strongylocentrotus intermedius is an economically important aquaculture species in northern China, where bacterial diseases significantly restrict healthy industry development. To counter these pathogens, specific pathogen-associated molecular patterns (PAMPs) are increasingly utilized as immunostimulants to prime the innate immune system. To elucidate the molecular mechanisms underlying early immune enhancement, a de novo transcriptome analysis was performed on sea urchins treated with lipopolysaccharides (LPS), peptidoglycan (PGN), and β-glucan. A total of 1007, 6216, and 2866 differentially expressed genes (DEGs) were identified in LPS, PGN, and β-glucan treatment group, respectively. Functional analyses demonstrated that LPS triggers an early MAPK signaling burst, PGN elicits a massive multi-pronged inflammatory signaling cascade, and β-glucan primarily activates cellular clearance mechanisms via lysosomal degradation pathways. These distinct upstream programs were further corroborated by rapid, stimulus-specific physiological responses, with extracellular SOD and CAT activities being selectively modulated under LPS and PGN stimulation. Notably, co-expression heatmap analyses revealed that these distinct pathway activations are tightly governed by specific, highly synchronized transcription factor (TF) modules. Among these, the transcription factor Runx1 was identified as a shared differentially expressed regulator whose expression was significantly correlated with the activation of numerous immune genes across all three PAMP treatment groups, suggesting that it functions as a universal core regulatory hub bridging these diverse immune stimuli. Despite these divergent upstream regulatory programs, analysis of 241 shared DEGs revealed that the immune response converges on a universal effector baseline centered on the phagosome, lysosome, NOD-like receptor (NLR) signaling, and primitive IL-17-driven pathways. Furthermore, the expression of key core immune genes, including RUNX1, IFITM, and MRC1, was successfully validated by qRT-PCR. These findings provide comprehensive insights into the early regulatory switches and conserved immune priming mechanisms in echinoderms, offering valuable molecular targets for the rational development of broad-spectrum immunostimulants in sea urchin aquaculture.</p>","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"61 ","pages":"102057"},"PeriodicalIF":2.4,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149225310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and evolutionary diversification of TRP channels in Yesso scallop (Patinopecten yessoensis).","authors":"Hongbo Lu, Xin Kang, Yuhui Liu, Chuanyan Yang, Qiuyu Zhang, Dongli Jiang, Lingling Wang, Linsheng Song","doi":"10.1016/j.cbd.2026.102056","DOIUrl":"https://doi.org/10.1016/j.cbd.2026.102056","url":null,"abstract":"<p><p>Transient receptor potential (TRP) channels are a conserved family of non-selective cation channels that play key roles in sensing diverse environmental stimuli. However, comprehensive genome-wide characterization and regulatory analyses of TRP gene families in aquatic mollusks particularly after high temperature stress remain limited. In this study, a comprehensive genome-wide analysis was conducted on TRP channels in Yesso scallop (Patinopecten yessoensis) to elucidate their structural feature, evolutionary relationships, and transcriptional response to high temperature stress. A total of 33 PyTRP genes were identified and classified into four subfamilies (PyTRPM, PyTRPV, PyTRPA and PyTRPC). Among them, PyTRPM proteins exhibited lineage-specific motif compositions with the unique presence of Motif 6. Their mRNAs exhibited distinct expression profiles across developmental stages and adult tissues. Under high temperature stress, PyTRPM3-4 and PyTRPM8 exhibited contrasting transcriptional responses. PyTRPM3-4 exhibited a stress-inducible expression pattern, characterized by rapid upregulation during high temperature stress and early recovery, followed by a gradual return to baseline levels. In contrast, PyTRPM8 maintained high basal expression but was rapidly repressed after high temperature stress, accompanied by temperature-dependent alternative splicing that was reversible during recovery. Different PyTRP subfamilies exhibited lineage-specific duplication, distinct duplication patterns and divergent expression profiles. Together, these findings reveal distinct high-temperature-associated expression patterns of PyTRP genes and a skipped-exon event in PyTRPM8 in P. yessoensis, providing a basis for future functional studies.</p>","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"61 ","pages":"102056"},"PeriodicalIF":2.4,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149225343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host defense peptide hepcidin 1 and iron overload differentially modulate energy, lipid, and hepatic-biliary metabolic pathways in gilthead seabream (Sparus aurata) blood.","authors":"Laura García-Navarro, Claudia Marín-Parra, Jhon A Serna Duque, María Ángeles Esteban","doi":"10.1016/j.cbd.2026.102050","DOIUrl":"https://doi.org/10.1016/j.cbd.2026.102050","url":null,"abstract":"<p><p>Hepcidin 1 (a host defense peptide with antimicrobial and iron-regulating properties) and iron overload are relevant in fish physiology; however, the in vivo effects of their increase in healthy fish are poorly characterised. This study evaluated the effects of intraperitoneal injections of hepcidin 1, iron (iron-dextran), and sequential treatment (iron and hepcidin 1) on the blood metabolome of gilthead seabream (Sparus aurata) after 10 days. Multivariate analyses revealed weak metabolomic changes in all three conditions. In all treatments, 15 chemical superclasses were conserved, with the predominance of lipids, indicating quantitative reorganisation. Energy metabolism and immune response were activated, involving lipids, fatty acids, acyl-CoA/acylcarnitines, and one‑carbon metabolism (folate/serine/methionine) linked to NAD(H)/NADP(H) and redox balance, although several associations lost robustness (non-significant FDR), consistent with the return to the homeostasis. Hepcidin 1 was associated with increased abundance of adenosine, Lys-Glu-Leu tripeptide, dodecanoylcarnitine, and phosphoethanolamines/phosphocholines (LysoPE/PE/LysoPC), and decreased levels of exogenous compounds (e.g. erythromycin C, harzianopyridone), suggesting modulation of the gut-microbiota-barrier axis or hepatic-biliary clearance. Iron overload-induced reprogramming is compatible with reactive oxygen species management, lipid remodelling, and mitochondrial fatty acid mobilisation, without markers of peroxidation, liver damage, or inflammation; herbicides (bensulfuron/sulfometuron methyl) were reduced. In the iron-hepcidin 1 group, PCA separation was not significant (p = 0.247), suggesting an overlap or no unidirectional shift in the metabolome. In conclusion, hepcidin 1 and iron modulate energy and lipid metabolism in gilthead seabream blood, providing biomarkers for interpreting immunometabolic adaptations, although targeted validation of annotations and higher temporal resolution are required for this purpose.</p>","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"61 ","pages":"102050"},"PeriodicalIF":2.4,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149229659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Dietary soybean or seaweed (Kappaphycus sp.) modulates taste-related gene (tas1r1 and tas1r2.2) expression in nile tilapia (Oreochromis niloticus)\" [Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, Volume 59 (2026), 101854].","authors":"Leonildo Dos Anjo Viagem, Jean Nepomuscene Hakizimana, Brunno da Silva Cerozi, Cyrus Rumisha, Gerald Misinzo","doi":"10.1016/j.cbd.2026.102012","DOIUrl":"https://doi.org/10.1016/j.cbd.2026.102012","url":null,"abstract":"","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":" ","pages":"102012"},"PeriodicalIF":2.4,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148893218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolutionary analysis of ghrelin in Actinopterygii.","authors":"Zhuoxin Lai, Hamad Khan, Lujun Chen, Jiahao Luo, Ming Li, Yusong Guo, Zhongduo Wang","doi":"10.1016/j.cbd.2025.101599","DOIUrl":"10.1016/j.cbd.2025.101599","url":null,"abstract":"<p><p>Ghrelin functions to stimulate appetite, promote the release of growth hormone, and regulate energy balance. Currently, research on the ghrelin is primarily focused on a single species, and there have been no systematic studies on the evolution of the ghrelin in fish. Therefore, this thesis conducts a comprehensive analysis of the ghrelin gene in 151 species of ray-finned bony fishes to reveal the universality and specificity of the ghrelin gene in the evolutionary history of fish, supplementing and perfecting the information on the ghrelin gene in Actinopterygii. The gene identification results show that the number of ghrelin genes varies among different fish species, 41 fish have lost the ghrelin gene, 98 fish having one ghrelin gene, and 12 fish having two ghrelin genes. Among the 110 fish species with the ghrelin gene, a total of 182 ghrelin gene sequences were identified, with transcript variant numbers ranging from 1 to 6, encoding 1 to 3 types of isoform proteins, and their mature peptides show a certain degree of similarity across different species. Phylogenetic analysis revealed that teleost ghrelin proteins segregate into three major evolutionary clades, with Salmoniformes orthologs comprising a distinct monophyletic cluster. The Cladistic and Chondrostei are clustered separately and then grouped with the more ancient Cypriniformes and Siluriformes species from the Neopterygii into a large group, while the other fish species from the Neopterygii form another large group. The Synteny analysis results indicate that the upstream gene of the ghrelin is CCDC174, and the downstream gene is TATDN2. The selection pressure analysis results show that there are no positive selection sites in the ghrelin gene, indicating that the ghrelin has been under strong functional constraint during the evolutionary process of fish. This study systematically investigates the evolutionary history of fish ghrelin, providing a theoretical basis for understanding the function and status of ghrelin in the feeding regulation system of fish, and deepening the recognition of its structural and functional evolution in the process of energy metabolism evolution.</p>","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"56 ","pages":"101599"},"PeriodicalIF":2.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144818622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms of kidney-mediated salinity adaptation in Takifugu obscurus, T. rubripes and their hybrid offspring.","authors":"Xinyan Liang, Dongkui Gao, Chenqi Wang, Yushun Tian, Qi Liu, Hongwei Yan, Rui Gao, Liu Wang, Meiyuan Li, Ruonan Zhang, Jinfeng Chen, Chen Jiang, Xiuli Wang","doi":"10.1016/j.cbd.2025.101659","DOIUrl":"10.1016/j.cbd.2025.101659","url":null,"abstract":"<p><p>Salinity changes significantly impact fish physiology, requiring efficient osmoregulation for survival. The kidney is vital for maintaining ion and water balance, crucial for internal stability in varying salinity. This study used transcriptomic analysis to examine molecular responses in the kidneys of Takifugu obscurus, T. rubripes, and their hybrids (Tor1 and Tor2) in freshwater (0 ppt) and seawater (32 ppt). Following the transfer from seawater to freshwater, all four fish groups showed consistent expression trends of three genes in their kidneys-one downregulated gene (ca7) and two upregulated genes (MAP1B and MUC4)-indicating their pivotal roles in renal osmoregulation. Additionally, RNA sequencing unveiled distinct transcriptional profiles specific to each species T. obscurus displayed a limited number of DEGs (14 upregulated and 4 downregulated) in freshwater, suggesting streamlined regulatory mechanisms consistent with its broad salinity tolerance. In contrast, T. rubripes exhibited more extensive transcriptional adjustments (449 upregulated and 139 downregulated), involving ion transport genes and metabolic pathways. Moreover, we observed significant changes in the expression of immune-related genes, indicating that variations in ambient salinity affect the immune responses of the four fish species. Genetic correlation analysis indicated that Tor1 clustered with T. rubripes, while Tor2 grouped with T. obscurus, implying that hybrid offspring inherit adaptive strategies from both parental lineages. These findings shed light on the molecular mechanisms governing kidney function in euryhaline fish and provide a theoretical foundation for breeding aquaculture species with enhanced stress tolerance.</p>","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"56 ","pages":"101659"},"PeriodicalIF":2.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145350424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis provides insights into gonad development and sex reversal of cultured largefin longbarbel catfish (Hemibagrus macropterus).","authors":"Huamei Yue, Ling Huang, Rui Ruan, Le Yu, Yong Xie, Chuangju Li, Huan Ye","doi":"10.1016/j.cbd.2025.101653","DOIUrl":"10.1016/j.cbd.2025.101653","url":null,"abstract":"<p><p>The largefin longbarbel catfish (Hemibagrus macropterus) is an important commercially cultured fish in southwestern China, whose regulatory mechanism of gonad development remains unknown. In this study, the first gonadal transcriptome sequencing of immature male, female, and intersexual individuals were performed. A total of 28,543 genes was annotated, of which 12,028, 6283 and 8019 differentially expressed genes (DEGs) were detected by pairwise comparisons of ovary versus (vs.) testis, ovary vs. intersex, and testis vs. intersex. Besides, 26 male-biased, 24 female-biased, and 7 intersex-biased DEGs were screened. Representative pathways related to gonadal development and sex reversal were further enriched. Interestingly, apart from the reproduction-related genes and pathways, apoptosis-related DEGs (bcl2, myc, caspase3 and tp53) and pathways such as JAK-STAT signaling pathway and P53 signaling pathway, were suggested to be involved in the sexual reversal process. The intersexual gonad might be developed by the sex change from ovary to testis, with transcriptions of female-biased genes reduced and male-biased genes increased. Relative real time PCR results of 14 DEGs verified the reliability of transcriptome data. These results will benefit our understanding of gonad development regulations, and further be useful for the achievement of improved artificial propagation of largefin longbarbel catfish.</p>","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"56 ","pages":"101653"},"PeriodicalIF":2.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145305039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gonadal transcriptome analysis of Cirrhina molitorella reveals sex-associated genes.","authors":"Weijian Chen, Guojun Cai, Weiqian Liang, Shengyue Lin, Weibin Li, Sixun Li, Binhua Deng, Mengmeng Wu, Chong Han, Qiang Li","doi":"10.1016/j.cbd.2025.101652","DOIUrl":"10.1016/j.cbd.2025.101652","url":null,"abstract":"<p><p>Cirrhina molitorella is an important economic fish species in southern China, and it holds a significant position among the highest-yielding fish species in this region. However, studies on the identification of sex-related genes and gonadal development in C. molitorella remain scarce, which has severely hindered the development of its aquaculture industry. In the present study, transcriptome sequencing data from the female and male gonads of C. molitorella were analyzed and compared for the first time, leading to the successful assembly of 64,954 unigenes. Through comparative transcriptomic analysis, a total of 20,310 differentially expressed genes were identified, among which 16,003 were significantly upregulated in testis and 4307 were highly expressed in ovaries. Furthermore, real-time quantitative PCR was also employed to validate the differential expression profiles of some genes, and the results were in agreement with the transcriptome data. These findings of this study can provide fundamental data for in-depth investigations into the functions of genes and signaling pathways associated with gender determination and gonadal formation in C. molitorella.</p>","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"56 ","pages":"101652"},"PeriodicalIF":2.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145254065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}