Comparative biochemistry and physiology. Part D, Genomics & proteomics最新文献_第2页

Effects of gonadotropin-releasing hormone analog (GnRHa) immunization on the gonadal transcriptome and proteome of tilapia (Oreochromis niloticus). 促性腺激素释放激素类似物(GnRHa)免疫对罗非鱼性腺转录组和蛋白质组的影响。

Comparative biochemistry and physiology. Part D, Genomics & proteomics Pub Date : 2020-03-31 DOI: 10.21203/rs.3.rs-18227/v1

Rui Wang, Luting Wen, Huawei Ma, Min Lv, Zhong Chen, Xuesong Du, Yong Lin, Huizan Yang

{"title":"Effects of gonadotropin-releasing hormone analog (GnRHa) immunization on the gonadal transcriptome and proteome of tilapia (Oreochromis niloticus).","authors":"Rui Wang, Luting Wen, Huawei Ma, Min Lv, Zhong Chen, Xuesong Du, Yong Lin, Huizan Yang","doi":"10.21203/rs.3.rs-18227/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-18227/v1","url":null,"abstract":"Gonadotropin releasing hormone (GnRH) plays an important role in the regulation of vertebrate reproduction. Studies have shown that immunization against GnRHa can induce sexually sterile tilapia. To explore the mechanism behind this, in this study, RNA-seq and data-independent acquisition (DIA) techniques were used to study the transcriptome and proteome of the gonad of tilapia immunized with GnRHa. 644 differentially expressed genes (80 upregulated and 564 downregulated) and 1150 differentially expressed proteins (351 upregulated and 799 downregulated) were identified. There were 209 genes with consistent differential expression patterns in the transcriptomic and proteomic analyses, of which 9 were upregulated and 200 downregulated, indicating that the gonad gene expression was inhibited by GnRHa immunization. The downregulated genes were particularly involved in the functions of single-organism process, binding, cellular process, metabolic process and catalytic activity, and associated with the pathways including ECM-receptor interaction, focal adhesion, cardiac muscle contraction and oxidative phosphorylation. The expression of six differentially expressed genes involved in the GnRH signaling pathway was all downregulated. In addition, several important functional genes related to gonadal development after GnRHa immunization were screened. This study confirmed the expression of corresponding genes was affected by GnRHa on the gonad development in tilapia at the molecular level, and laid a foundation for elucidating the mechanism of GnRHa immunization.","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"52 1","pages":"100780"},"PeriodicalIF":0.0,"publicationDate":"2020-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88945122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Early life stress causes persistent impacts on the microbiome of Atlantic salmon 早期的生活压力会对大西洋鲑鱼的微生物群产生持续的影响

Comparative biochemistry and physiology. Part D, Genomics & proteomics Pub Date : 2020-01-17 DOI: 10.1101/2020.01.16.908939

T. U. Webster, S. Consuegra, C. G. de Leaniz

{"title":"Early life stress causes persistent impacts on the microbiome of Atlantic salmon","authors":"T. U. Webster, S. Consuegra, C. G. de Leaniz","doi":"10.1101/2020.01.16.908939","DOIUrl":"https://doi.org/10.1101/2020.01.16.908939","url":null,"abstract":"Intensively farmed fish are commonly stressed, often leading to immune impairment and increased susceptibility to disease. Microbial communities associated with the gut and skin are vital to host immune function, but little is known about how stress affects the fish microbiome, especially during the sensitive early life stages. We compared the effects of two aquaculture-relevant stressors on the gut and skin microbiome of Atlantic salmon fry: an acute cold stress during late embryogenesis, and a chronic environmental stress during the larval stage. Acute cold stress had a lasting effect on the structure of both the gut and the skin microbiome, likely due to disruption of the egg shell microbial communities which seed the initial colonisation of the teleost microbiome upon hatching. In contrast, chronic post hatch stress altered the structure of the gut microbiome, but not that of the skin. Both types of stressors promoted similar Gammaproteobacteria ASVs, particularly within the genera Acinetobacter and Aeromonas which include several important fish pathogens and, in the gut, reduced the abundance of Lactobacillales. This suggests that there may be common signatures of stress in the salmon microbiome, which may represent useful stress biomarkers in aquaculture.","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"248 1","pages":"None - None"},"PeriodicalIF":0.0,"publicationDate":"2020-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88348122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Phylogenomic analysis of transferrin family from animals and plants. 动植物转铁蛋白家族的系统基因组分析。

Comparative biochemistry and physiology. Part D, Genomics & proteomics Pub Date : 2016-03-01 DOI: 10.1016/j.cbd.2015.11.002

Lina Bai, M. Qiao, R. Zheng, Changyan Deng, S. Mei, Wanping Chen

引用次数: 18

Identification and comparative analysis of the oriental river prawn (Macrobrachium nipponense) microRNA expression profile during hypoxia using a deep sequencing approach. 利用深度测序方法鉴定日本沼虾(Macrobrachium nipponense)缺氧状态下的microRNA表达谱并进行比较分析。

Comparative biochemistry and physiology. Part D, Genomics & proteomics Pub Date : 2016-03-01 DOI: 10.1016/j.cbd.2016.01.003

Shengming Sun, H. Fu, X. Ge, Jian Zhu, Z. Gu, Fujun Xuan

引用次数: 19