性别问题:斑马鱼父母暴露于缺氧环境后microRNA的配子特异性贡献。

W. Heinrichs-Caldas, H. Ikert, V. M. Almeida-Val, P. M. Craig
{"title":"性别问题:斑马鱼父母暴露于缺氧环境后microRNA的配子特异性贡献。","authors":"W. Heinrichs-Caldas, H. Ikert, V. M. Almeida-Val, P. M. Craig","doi":"10.2139/ssrn.4341895","DOIUrl":null,"url":null,"abstract":"Oxygen availability varies among aquatic environments, and oxygen concentration has been demonstrated to drive behavioral, metabolic, and genetic adaptations in numerous aquatic species. MicroRNAs (miRNAs) are epigenetic modulators that act at the interface of the environment and the transcriptome and are known to drive plastic responses following environmental stressors. An area of miRNA that has remained underexplored is the sex specific action of miRNAs following hypoxia exposure and its effects as gene expression regulator in fishes. This study aimed to identify differences in mRNA and miRNA expression in the F1 generation of zebrafish (Danio rerio) at 1 hpf after either F0 parental male or female were exposed to 2 weeks of continuous (45 %) hypoxia. In general, F1 embryos at 1 hpf demonstrated differences in mRNA and miRNAs expression related to the stressor and to the specific sex of the F0 that was exposed to hypoxia. Bioinformatic pathway analysis of predicted miRNA:mRNA relationships indicated responses in known hypoxia signaling and mitochondrial bioenergetic pathways. This research demonstrates the importance of examining the specific male and female contributions to phenotypic variation in subsequent generations and provides evidence that there is both maternal and paternal contribution of miRNA through eggs and sperm.","PeriodicalId":93949,"journal":{"name":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","volume":"20 1","pages":"101090"},"PeriodicalIF":0.0000,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Sex matters: Gamete-specific contribution of microRNA following parental exposure to hypoxia in zebrafish.\",\"authors\":\"W. Heinrichs-Caldas, H. Ikert, V. M. Almeida-Val, P. M. Craig\",\"doi\":\"10.2139/ssrn.4341895\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Oxygen availability varies among aquatic environments, and oxygen concentration has been demonstrated to drive behavioral, metabolic, and genetic adaptations in numerous aquatic species. MicroRNAs (miRNAs) are epigenetic modulators that act at the interface of the environment and the transcriptome and are known to drive plastic responses following environmental stressors. An area of miRNA that has remained underexplored is the sex specific action of miRNAs following hypoxia exposure and its effects as gene expression regulator in fishes. This study aimed to identify differences in mRNA and miRNA expression in the F1 generation of zebrafish (Danio rerio) at 1 hpf after either F0 parental male or female were exposed to 2 weeks of continuous (45 %) hypoxia. In general, F1 embryos at 1 hpf demonstrated differences in mRNA and miRNAs expression related to the stressor and to the specific sex of the F0 that was exposed to hypoxia. Bioinformatic pathway analysis of predicted miRNA:mRNA relationships indicated responses in known hypoxia signaling and mitochondrial bioenergetic pathways. This research demonstrates the importance of examining the specific male and female contributions to phenotypic variation in subsequent generations and provides evidence that there is both maternal and paternal contribution of miRNA through eggs and sperm.\",\"PeriodicalId\":93949,\"journal\":{\"name\":\"Comparative biochemistry and physiology. Part D, Genomics & proteomics\",\"volume\":\"20 1\",\"pages\":\"101090\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative biochemistry and physiology. Part D, Genomics & proteomics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2139/ssrn.4341895\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative biochemistry and physiology. Part D, Genomics & proteomics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2139/ssrn.4341895","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

氧的可用性在不同的水生环境中是不同的,氧浓度已经被证明可以驱动许多水生物种的行为、代谢和遗传适应。MicroRNAs (miRNAs)是一种表观遗传调节剂,在环境和转录组的界面上起作用,并在环境应激源下驱动可塑性反应。miRNA的一个尚未被充分探索的领域是miRNA在缺氧暴露后的性别特异性作用及其作为基因表达调节剂在鱼类中的作用。本研究旨在鉴定1 hpf时F1代斑马鱼(Danio rerio)在F0亲代雄性或雌性连续暴露于2周(45%)缺氧后mRNA和miRNA表达的差异。总的来说,1hpf的F1胚胎表现出与应激源相关的mRNA和mirna的表达差异以及暴露于缺氧的F0的特定性别。预测miRNA的生物信息学途径分析:mRNA关系显示了已知缺氧信号和线粒体生物能量途径的反应。这项研究证明了在后代中检查雄性和雌性对表型变异的特定贡献的重要性,并提供了证据,证明母亲和父亲通过卵子和精子都有miRNA的贡献。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Sex matters: Gamete-specific contribution of microRNA following parental exposure to hypoxia in zebrafish.
Oxygen availability varies among aquatic environments, and oxygen concentration has been demonstrated to drive behavioral, metabolic, and genetic adaptations in numerous aquatic species. MicroRNAs (miRNAs) are epigenetic modulators that act at the interface of the environment and the transcriptome and are known to drive plastic responses following environmental stressors. An area of miRNA that has remained underexplored is the sex specific action of miRNAs following hypoxia exposure and its effects as gene expression regulator in fishes. This study aimed to identify differences in mRNA and miRNA expression in the F1 generation of zebrafish (Danio rerio) at 1 hpf after either F0 parental male or female were exposed to 2 weeks of continuous (45 %) hypoxia. In general, F1 embryos at 1 hpf demonstrated differences in mRNA and miRNAs expression related to the stressor and to the specific sex of the F0 that was exposed to hypoxia. Bioinformatic pathway analysis of predicted miRNA:mRNA relationships indicated responses in known hypoxia signaling and mitochondrial bioenergetic pathways. This research demonstrates the importance of examining the specific male and female contributions to phenotypic variation in subsequent generations and provides evidence that there is both maternal and paternal contribution of miRNA through eggs and sperm.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信