Canadian journal of diabetes最新文献

{"title":"A Qualitative Exploration of Diabetes Professionals' Perspectives on Social Determinants of Health Among Black and South Asian With Type 2 Diabetes in Ontario.","authors":"Maura Eswaradas, Rachael Tontodonati, Christine L Sheppard","doi":"10.1016/j.jcjd.2026.06.005","DOIUrl":"10.1016/j.jcjd.2026.06.005","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate how diabetes-related professionals perceive the impact of social determinants of health on diabetes outcomes among Black and South Asian populations in Ontario and to identify perceived opportunities and gaps in current responses.</p><p><strong>Methods: </strong>A qualitative study was conducted using stakeholder consultation data from 5 focus groups (n=21) with both frontline (individuals directly engaged in delivering diabetes care services) and population-level professionals (individuals working in public health settings or in systems-level roles that influence diabetes care delivery) across Ontario. Reflexive thematic analysis was used to construct key themes.</p><p><strong>Results: </strong>Participants identified food insecurity, health care access, inadequate housing, and social support as major factors affecting diabetes outcomes for Black and South Asian populations. Although individual-level interventions provided short-term support, they were constrained by funding limitations and failed to address upstream structural barriers. Frontline professionals emphasized the need for population-level action to relieve the burden on community organizations in filling systemic gaps.</p><p><strong>Conclusions: </strong>Findings underscore the need for systems-level policies targeting upstream social determinants to support equitable diabetes prevention and management in Ontario among Black and South Asian populations.</p>","PeriodicalId":93918,"journal":{"name":"Canadian journal of diabetes","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148383088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultural Adaptation, Translation, and Psychometric Validation of the Chinese Diabetes Education Questionnaire (DATE-Q-Chinese).","authors":"Jennifer Xiao, Gabriela Lima de Melo Ghisi, Anne Cao, Po-Lin Cheung-Leung, Louise Poon, LaiNam Yip, Alvita Chan, Tamara Belfer, Paul Oh, Crystal Aultman, Yanting Huang, Grace Lee, Calvin Ke","doi":"10.1016/j.jcjd.2026.07.003","DOIUrl":"10.1016/j.jcjd.2026.07.003","url":null,"abstract":"<p><strong>Objective: </strong>To culturally adapt and translate the DiAbeTes Education Questionnaire (DATE-Q) into Chinese (DATE-Q-Chinese) and to psychometrically validate the DATE-Q-Chinese for Chinese-speaking adults with type 2 diabetes (T2D).</p><p><strong>Methods: </strong>We culturally adapted and translated the DATE-Q using a structured framework. We measured content and face validity among 7 care providers and 7 Chinese people with T2D. We conducted a multi-site, cross-sectional survey at outpatient clinics in Toronto, Canada (2024 to 2025) for psychometric validation of the DATE-Q-Chinese among 103 Cantonese- and/or Mandarin-speaking adults aged ≥18 years with T2D, excluding those who were pregnant or had conditions that precluded questionnaire completion. We conducted exploratory factor analysis, calculated Cronbach's alpha (internal consistency), measured the correlation between DATE-Q-Chinese and Health Literacy index scores (criterion validity), and conducted analyses of variance to compare DATE-Q-Chinese scores across educational levels (construct validity).</p><p><strong>Results: </strong>Mean content validity and face validity scores were 4.7 (standard deviation 0.6) and 4.7 (0.5) of 5. Validation study participants were 35.9% female (mean age: 63.5 [12.6] years). Five factors matching the original DATE-Q domains (self-management, long-term complications, being active, healthy eating, psychosocial well-being) explained 64.2% of the total variance. Cronbach's alpha was 0.84 (range: 0.70 to 0.85 across domains). The total DATE-Q-Chinese score (mean: 13.4 [3.8] of 20) was positively correlated with health literacy (r=0.269, p=0.006) and increased with education level (p=0.01). The \"healthy eating\" items had the highest mean score (3.5 [0.9]) and least \"I don't know responses\" (range: 6.8% to 10.7% across items), whereas \"self-management\" items had the lowest mean score (1.8 [1.2]) and most \"I don't know\" responses (range: 9.7% to 41.7%).</p><p><strong>Conclusions: </strong>The DATE-Q-Chinese is reliable and valid for assessing T2D-related knowledge among Chinese adults with T2D who attended routine diabetes care.</p>","PeriodicalId":93918,"journal":{"name":"Canadian journal of diabetes","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148681444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glucagon-like Peptide-1 Receptor Agonist Therapy in Cystic Fibrosis-Related Diabetes: Insights From a Pilot Implementation Program.","authors":"Mirna Gerges, Carly Nichols, Kenzie MacDuff, Barna De, Meredith Chiasson","doi":"10.1016/j.jcjd.2026.06.001","DOIUrl":"10.1016/j.jcjd.2026.06.001","url":null,"abstract":"<p><p>Management of cystic fibrosis (CF)-related diabetes (CFRD) is evolving as the CF population experiences increasing rates of overweight and obesity, particularly with the widespread use of CF transmembrane conductance regulator (CFTR) modulators. This article describes practical insights from implementing a pilot program that evaluated injectable semaglutide, a glucagon-like peptide-1 receptor agonist (GLP-1 RA), in adults with CFRD. Our program followed 11 participants over 12 months, revealing important considerations for patient selection, monitoring protocols, and managing adverse effects in this unique population. Although most participants tolerated semaglutide well with clinically meaningful improvements observed in glycemic management, careful screening and close follow-up proved essential. These findings offer practical guidance for diabetes educators and clinicians considering GLP-1 RA therapy as an adjunct to insulin in carefully selected patients with CFRD.</p>","PeriodicalId":93918,"journal":{"name":"Canadian journal of diabetes","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148304298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterizing Subjective Sleep Quality and Sleep Apnea Risk in Adults With Type 1 Diabetes.","authors":"Miranda Zuniga-Kennedy, Devon Hansen, Jane D Bulger, Elizabeth Grinspoon, Laneé Jung, Yogish C Kudva, Luciana Mascarenhas Fonseca, Richard Pratley, Michael R Rickels, Shafaq R Rizvi, Martin Sliwinski, Ruth S Weinstock, Laura T Germine, Naomi Chaytor","doi":"10.1016/j.jcjd.2026.06.003","DOIUrl":"10.1016/j.jcjd.2026.06.003","url":null,"abstract":"<p><strong>Objective: </strong>Individuals with type 1 diabetes (T1D) experience poor subjective sleep quality and are at an increased risk for obstructive sleep apnea (OSA), yet the contribution of diabetes-related characteristics remains unclear.</p><p><strong>Methods: </strong>This study evaluated associations between diabetes characteristics, affect, and sleep in 210 adults with T1D (M age=45.6 years, SD=15.6; 52.9% female) from the Glycemic Variability and Fluctuations in Cognitive Status in Adults with Type 1 Diabetes study. Participants completed measures of sleep quality (Pittsburgh Sleep Quality Index), OSA risk (STOP-Bang), and affect (Generalized Anxiety Disorder-7, Perceived Stress Scale, Patient Health Questionnaire-8). Blinded continuous glucose monitoring data were collected for up to 20 days (M=18.8, SD=2.5). Bivariate correlations and hierarchical regressions evaluated relationships among subjective sleep quality and OSA risk with affect and diabetes variables.</p><p><strong>Results: </strong>Overall, 47% and 37% of participants scored above clinical cutoffs on the Pittsburgh Sleep Quality Index and STOP-Bang. Those with microvascular complications reported poorer sleep quality (β=0.13, t(188)=2.23, p=0.03). Microvascular complications (β=0.15, t(200)=3.10, p=0.002) and cardiovascular disease (β=.014, t(200)=2.89, p=0.004) were associated with a higher OSA risk. Continuous glucose monitoring metrics were not associated with the outcomes.</p><p><strong>Conclusions: </strong>In this sample of adults with T1D, nearly half reported poor sleep quality, and more than one-third were at elevated risk for OSA, with microvascular complications associated with poorer sleep quality and both microvascular and cardiovascular complications associated with a higher OSA risk. Aggregate glycemic metrics were not associated with sleep outcomes. Future research should evaluate the relationships between daily variation in sleep and glucose to inform targeted clinical interventions.</p>","PeriodicalId":93918,"journal":{"name":"Canadian journal of diabetes","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148383160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Defining a Clinically Meaningful Cut Point for the Hypoglycemia Fear Survey-Parents of Young Children.","authors":"Susana R Patton, Nicole Kahhan, Holly K O'Donnell, Jacob Redel, Kimberly A Driscoll","doi":"10.1016/j.jcjd.2026.07.002","DOIUrl":"10.1016/j.jcjd.2026.07.002","url":null,"abstract":"<p><strong>Objective: </strong>Parents of young children with type 1 diabetes (T1D) frequently experience fear of hypoglycemia (FH), which contributes to higher child glucose levels. The Hypoglycemia Fear Survey-Parents of Young Children (HFS-PYC) is a validated questionnaire used to assess parental FH, yet it lacks a clinically interpretable cut point. We sought to identify a preliminary HFS-PYC cut point that associates with higher parental anxiety, greater diabetes distress, and child glucose metrics.</p><p><strong>Methods: </strong>Parents (n=180) completed the HFS-PYC, PROMIS-Anxiety Short Form (PROMIS-A), and Problem Areas in Diabetes-Parent Revised (PAID-PR). We derived candidate cut points using 1) modal score distribution (mode), 2) mean plus 1 standard deviation (SD), and 3) an elevated-item rule (EI). We compared groups above vs below each cut point on PROMIS-A, PAID-PR, and child glycemic metrics. We calculated indices of diagnostic accuracy using PROMIS-A T-scores ≥60 as a reference.</p><p><strong>Results: </strong>Candidate cut points were ≥61 (mode) and ≥77 (SD); EI required ≥5 of 9 items rated \"often/almost always.\" The prevalence of elevated FH ranged from 52% (mode) to 18% (SD). All cut points distinguished between parents with high vs low PROMIS-A and PAID-PR scores (p<0.001). Only the mode cut point differentiated among parents for some child glycemic metrics. The mode cut point also had the most favourable diagnostic accuracy (sensitivity=0.77, specificity=0.57) among the candidate cut points.</p><p><strong>Conclusions: </strong>An HFS-PYC total score ≥61 identifies parents with greater anxiety and diabetes distress and children with higher glycemic metrics and demonstrates the best diagnostic performance among candidate cut points. Pending further validation, this preliminary cut point may offer a practical starting point for clinical screening and research.</p>","PeriodicalId":93918,"journal":{"name":"Canadian journal of diabetes","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148551021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N-Glycosylation of Cav3.2 Channel in Myelinated A-Fibre Dorsal Root Ganglion Neurons Contributes to Mechanical Allodynia in Diabetic Rats.","authors":"Chenlong Liao, Shuo Li, Wenchuan Zhang","doi":"10.1016/j.jcjd.2026.07.001","DOIUrl":"10.1016/j.jcjd.2026.07.001","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the role of Cav3.2 channel N-glycosylation in myelinated A-fibre dorsal root ganglion (DRG) neurons in the development of diabetic mechanical allodynia (MA).</p><p><strong>Methods: </strong>Type 1 diabetes was induced in rats via a single intraperitoneal injection of streptozotocin. Pain behaviour, including MA and thermal hyperalgesia (TH), was assessed weekly. Diabetic rats with TH were further divided into 2 subgroups according to the presence of MA by the third week. The impact of N-glycosylation on Cav3.2 expression and pain behaviours was investigated through sequential intraplantar administration of neuraminidase (NEU) and TTA-P2, a selective blocker of T-type calcium channels. The Cav3.2 N-glycosylation levels were compared between diabetic rats with and without MA by analyzing the N-terminal fragment after enzymatic deglycosylation.</p><p><strong>Results: </strong>Cav3.2 expression in myelinated A-fibre DRG neurons was significantly higher in diabetic rats with MA than in those without (p<0.001). NEU-induced deglycosylation reduced Cav3.2 expression in all groups, although diabetic rats with MA still had higher expression than those without (p<0.05). Resiniferatoxin eliminated TH but not MA, whereas NEU alleviated both, similar to TTA-P2. Diabetic rats with MA exhibited increased expression of Cav3.2 N-terminal fragments compared with those without (p<0.001).</p><p><strong>Conclusion: </strong>N-glycosylation of the Cav3.2 channel in DRG neurons of myelinated A-fibres contributes to diabetic MA.</p>","PeriodicalId":93918,"journal":{"name":"Canadian journal of diabetes","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148474568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Sensor-Derived Daily Glycemic Variability in Treatment-Requiring Diabetic Ocular Disease: A Retrospective Case-Control Study.","authors":"Kelly Ann Hutchinson, Olivia Calancie, Patrick Wang, Sara Awad, Robert J Campbell, Manpartap Bal","doi":"10.1016/j.jcjd.2026.06.008","DOIUrl":"10.1016/j.jcjd.2026.06.008","url":null,"abstract":"<p><strong>Objective: </strong>Daily glycemic variability (GV) has been proposed as a contributor to diabetic ocular complications beyond chronic glycemic management. This study evaluated whether sensor-derived GV (coefficient of variation, %CV) is associated with treatment-requiring diabetic ocular disease.</p><p><strong>Methods: </strong>Retrospective case-control study of adults with diabetes followed by Endocrinology departments at 2 tertiary hospitals in Kingston, Ontario. Inclusion criteria were as follows: diabetes duration ≥5 years, age ≥18 years, continuous glucose monitoring (CGM) wear ≥70% in a continuous 90-day period (Jan 2023-May 2024), and a documented eye examination (Jan 2023-Dec 2024). Cases were identified as having received diabetic ocular treatment (intravitreal injection [IVI], retinal laser, and/or pars plana vitrectomy) using OHIP codes (2015-2024) and confirmed by blinded ophthalmology chart review; controls had not received such treatment. Device-reported 14- and 90-day CGM metrics were extracted. In cases treated between 2022 and 2024, IVI burden was compared by 90-day %CV (≤36 vs >36) and correlated with CGM metrics.</p><p><strong>Results: </strong>Among 485 patients, 72 (14.8%) were cases. Cases were older and had a longer diabetes duration than controls (both p<0.0001). There were no differences between cases and controls in 14- or 90-day GV (%CV), mean glucose, or time in range. In 57 recently treated cases (2022-2024), IVI burden was higher in the low %CV group than in the high %CV group (9.9 vs 6.3; p=0.046). %CV had a weak negative, nonsignificant correlation with IVI count, whereas time in range correlated positively with IVI count (r=0.351; p=0.012).</p><p><strong>Conclusions: </strong>Short-term CGM-derived GV was not associated with treatment-requiring diabetic ocular disease. Within recently treated patients, GV-treatment relationships likely reflect confounding by care intensity and disease stage.</p>","PeriodicalId":93918,"journal":{"name":"Canadian journal of diabetes","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148440044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remission Possible: Determining the Impact of Targeted Social Media Campaigns to Promote Type 2 Diabetes Remission Awareness.","authors":"Alexis Marcotte-Chénard, Denham Hachey, Barbara Oliveira, Damien Gillis, Amrit Gill, Sean Mckelvey, Anne Mullens, Barb Reece, J J Bélanger, Chris Xi, Amy Morrisey, Jonathan P Little","doi":"10.1016/j.jcjd.2026.06.006","DOIUrl":"10.1016/j.jcjd.2026.06.006","url":null,"abstract":"<p><strong>Objective: </strong>The possibility of achieving type 2 diabetes (T2D) remission is a relatively new concept and remains unknown to most people affected by T2D. To evaluate the impact (including reach, engagement, conversation rate, cost-per-click, and website engagement) of paid, targeted social media advertising featuring inspiring stories of persons with lived experience (PWLE) and still-frame advertisements as knowledge mobilization strategies to increase awareness of T2D remission.</p><p><strong>Methods: </strong>In collaboration with PWLE, we developed the \"Remission Possible\" social media campaign, consisting of still-frame images and storytelling video advertisements, to highlight T2D remission and direct interested users to a website (Diabetesremission.ca) that explains the concept and provides evidence-informed resources.</p><p><strong>Results: </strong>During the paid social media campaigns, we reached 235,561 unique individuals (70% female, most being >45 years old), resulting in 6,250 people accessing our website, with an average conversation rate of 2.7% and a cost-per-click of $0.36. Of these, 1,135 individuals took meaningful action (e.g. downloading material). Compared with organic posts, paid advertisements increased visitors to our website (organic posts: 10±9 users per day; paid posts: 180±72 users per day; p=0.0001; D=2.08), with no difference in the time spent on the website (7.0±6.6 min) for visitors from paid vs organic posts (p=0.576). Still-frame images designed to evoke stronger emotions decreased the cost-per-click compared with neutral advertisements ($0.29 vs $0.41). Storytelling videos were similarly effective, especially when using a short vertical format (Reels).</p><p><strong>Conclusion: </strong>Targeted social media advertising seems to be an effective way to promote T2D remission awareness among potentially interested individuals, while also demonstrating a favorable cost per click.</p>","PeriodicalId":93918,"journal":{"name":"Canadian journal of diabetes","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148383328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal Serum Polyol Levels Associated With Gestational Diabetes Mellitus and Large-for-Gestational-Age Infants: A Population-Based Nested Case-Control Study.","authors":"Tao Tan, Meng Wang, Yicheng Qi, Yu Liu, Li Qin, Jing Ma","doi":"10.1016/j.jcjd.2026.06.004","DOIUrl":"10.1016/j.jcjd.2026.06.004","url":null,"abstract":"<p><strong>Objective: </strong>Polyols, natural nutritive sweeteners, are often found in the diet during pregnancy. This study investigates the association between maternal serum polyol levels and the risk of gestational diabetes mellitus (GDM) and large-for-gestational-age (LGA) infants.</p><p><strong>Methods: </strong>This nested case-control study matched 218 women (with GDM vs. without GDM) by age and body mass index. After exclusions, the final analytic sample comprised 194 women for glucose metabolism outcomes and 177 women for birth weight and LGA outcomes. Serum polyols (sorbitol, erythritol, xylitol, and maltitol) were quantified using gas chromatography coupled with time-of-flight mass spectrometry. LGA was defined as a birth weight ≥90th percentile or ≥4000 g. Logistic regression was used to assess the associations between polyols and GDM and LGA, with adjustment for confounders.</p><p><strong>Results: </strong>Among the participants, 23.7% had LGA infants, and maternal serum polyol levels were significantly higher in this group. After adjusting for confounders, a 1-standard deviation increase in serum polyol levels was associated with increased odds of LGA (odds ratio=1.71, 95% confidence interval [CI]:1.22 to 2.40) and GDM (OR=1.52, 95% CI: 1.09 to 2.14). Erythritol and sorbitol were significantly associated with the odds of LGA, whereas xylitol and maltitol had no significant associations. Furthermore, a 1-standard deviation increase in serum polyol levels was linked to higher birth weight (beta [β]=95 g, 95% CI: 33 to 157 g) and elevated homeostasis model assessment for insulin resistance (β=0.32, 95% CI: 0.12 to 0.52).</p><p><strong>Conclusions: </strong>Elevated maternal serum polyol levels were positively associated with the risk of GDM, LGA infants, and higher birth weight.</p>","PeriodicalId":93918,"journal":{"name":"Canadian journal of diabetes","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148383318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced Glycation Endproducts Delay Diabetic Wound Healing by OSR2/SPRR1B Signalling in Keratinocyte's Proliferation and Differentiation.","authors":"Jiaoyun Dong, Xiaozan Cao, Yuzhi Jiang, Zengding Zhou, Ming Tian","doi":"10.1016/j.jcjd.2026.06.002","DOIUrl":"10.1016/j.jcjd.2026.06.002","url":null,"abstract":"<p><strong>Objective: </strong>The deposition of advanced glycation endproducts (AGEs) in skin tissue is a major risk factor leading to refractory diabetic wounds. However, the mechanism of AGEs in diabetic wounds remains unclear. This study aimed to investigate the mechanism and signalling pathway of AGEs in diabetic wound healing.</p><p><strong>Methods: </strong>This study integrated bioinformatics analysis of the Gene Expression Omnibus data set (GSE80178) to identify differentially expressed genes between diabetic foot ulcer and non-diabetic foot ulcer tissues, with small proline-rich protein 1B (SPRR1B) validated as a key target in clinical specimens. Mechanistic investigations were conducted using AGE-stimulated HaCaT cells and db/db diabetic mouse models. We combined cellular functional assays (CCK-8, flow cytometry, western blotting, and immunofluorescence), chromatin immunoprecipitation, and in vivo short hairpin RNA knockdown to explore how the OSR2-SPRR1B axis regulates cell fate and affects diabetic wound healing.</p><p><strong>Results: </strong>SPRR1B and OSR2 levels were upregulated in diabetic wound tissues and HaCat cells with AGE intervention. After knockdown of SPRR1B or OSR2 expression, keratinocyte proliferation was promoted and apoptosis was inhibited, the expression of CK5/CK14 and loricrin increased, and the expression of CK1/CK10 decreased significantly. SPRR1B expression was changed by OSR2 knockdown and overexpression. SPRR1B expression significantly increased with OSR2 inducing directly, as revealed by chromatin immunoprecipitation assays. SPRR1B, OSR2, and AGEs were highly expressed in db/db mice. Knockdown of SPRR1B accelerated diabetic wound healing, increased the Ki67-positive cell rate, decreased the apoptosis rate, increased CK5/CK14 expression in the basal layer and CK1/CK10 between the basal layer and horny layer, and increased loricrin in epidermal tissue in db/db mice.</p><p><strong>Conclusions: </strong>SPRR1B was a key factor in diabetic wounds with AGE deposition, and OSR2 was an upstream control gene of SPRR1B. Inhibition of SPRR1B expression enhanced diabetic wound healing.</p>","PeriodicalId":93918,"journal":{"name":"Canadian journal of diabetes","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148285016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}